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公开(公告)号：US20110059525A1
公开(公告)日：2011-03-10
申请号：US12818034
申请日：2010-06-17
CPC分类号： C12N15/63 , C07K2319/71 , C07K2319/715 , C07K2319/80 , C12N15/1055 , C12N15/85 , C12N2510/00 , C12N2830/002 , C12N2830/15
摘要： This invention relates to the field of biotechnology or genetic engineering. Specifically, this invention relates to the field of gene expression. More specifically, this invention relates to a novel inducible gene expression system and methods of modulating gene expression in a host cell for applications such as gene therapy, large scale production of proteins and antibodies, cell-based high throughput screening assays, functional genomics and regulation of traits in transgenic plants and animals.
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公开(公告)号：US07776587B2
公开(公告)日：2010-08-17
申请号：US11841495
申请日：2007-08-20
CPC分类号： C12N15/63 , C07K2319/71 , C07K2319/715 , C07K2319/80 , C12N15/1055 , C12N15/85 , C12N2510/00 , C12N2830/002 , C12N2830/15
摘要： This invention relates to the field of biotechnology or genetic engineering. Specifically, this invention relates to the field of gene expression. More specifically, this invention relates to a novel inducible gene expression system and methods of modulating gene expression in a host cell for applications such as gene therapy, large scale production of proteins and antibodies, cell-based high throughput screening assays, functional genomics and regulation of traits in transgenic plants and animals.
摘要翻译： 本发明涉及生物技术或遗传工程领域。 具体地，本发明涉及基因表达领域。 更具体地，本发明涉及一种新的诱导型基因表达系统和调节宿主细胞中基因表达的方法，用于诸如基因治疗，蛋白质和抗体的大规模生产，基于细胞的高通量筛选测定，功能基因组学和调节 的转基因植物和动物的性状。
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13.发明授权Hybrid polypeptide comparing a biotinylated avidin binding polypeptide fused to a polypeptide of interest 失效杂交多肽比较与感兴趣的多肽融合的生物素化的抗生物素蛋白结合多肽
公开(公告)号：US6072039A
公开(公告)日：2000-06-06
申请号：US687819
申请日：1991-04-19
IPC分类号： A61K38/00 , A61K39/00 , A61P35/00 , C07K1/22 , C07K7/04 , C07K14/00 , C07K14/005 , C07K14/195 , C07K14/675 , C07K19/00 , C12N9/10 , C12N15/09 , C12N15/62 , C12P21/02 , C12R1/19 , C12R1/91 , G01N33/53 , G01N33/574 , C12N15/00
CPC分类号： C12N15/62 , C07K14/675 , C12N9/1018 , C07K2319/00 , C07K2319/75
摘要： A hybrid polypeptide is disclosed, comprising a polypeptide for attachment of a prosthetic group to avidin fused to at least one polypeptide of interest, and a method of making the same. The hybrid polypeptide is produced by recombinant DNA techniques, using a DNA expression vector composed of a DNA fragment coding for the polypeptide for attachment to avidin fused to DNA coding for one or more polypeptides of interest. The hybrid polypeptide may also contain linking amino acid sequences for cleavage of the polypeptide of interest from the polypeptide for attachment using an appropriate proteolytic or chemical reagent. The hybrid polypeptide is expressed in appropriate host cells transformed with the DNA expression vector encoding the hybrid polypeptide, and may be recovered from crude cell extracts in high yield and high purity using avidin affinity chromatography. Following avidin affinity purification, the polypeptide for attachment and polypeptide of interest may be cleaved to yield polypeptide of interest in a highly pure and highly active state.
摘要翻译： 公开了一种杂合多肽，其包含用于将假基团连接至与至少一种目标多肽融合的抗生物素蛋白的多肽及其制备方法。 通过重组DNA技术，使用由编码多肽的DNA片段组成的DNA表达载体来产生杂交多肽，所述DNA片段与用于编码一种或多种感兴趣多肽的DNA融合的抗生物素蛋白连接。 杂合多肽还可以含有用于使用合适的蛋白水解或化学试剂从所述多肽切割感兴趣多肽的连接氨基酸序列用于连接。 杂交多肽在用编码杂合多肽的DNA表达载体转化的合适的宿主细胞中表达，并且可以使用亲和素亲和层析以高产率和高纯度从粗细胞提取物中回收。 在亲和素亲和纯化之后，可以切割用于附着的多肽和目标多肽，以在高纯度和高活性状态下产生感兴趣的多肽。
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14.发明授权公开(公告)号：US09063140B2
公开(公告)日：2015-06-23
申请号：US13174620
申请日：2011-06-30
IPC分类号： C07H21/04 , C12N15/62 , G01N33/566 , C07K14/435
CPC分类号： G01N33/566 , C07K14/43577 , G01N2333/723
摘要： The present invention relates to a novel isolated whitefly ecdysone receptor polypeptide. The invention also relates to an isolated nucleic acid encoding the whitefly ecdysone receptor polypeptide, to vectors comprising them and to their uses, in particular in methods for modulating gene expression in an ecdysone receptor-based gene expression modulation system and methods for identifying molecules that modulate whitefly ecdysone receptor activity.
摘要翻译： 本发明涉及一种新型的孤立的粉虱蜕皮激素受体多肽。 本发明还涉及编码粉虱蜕皮激素受体多肽的分离的核酸，包含它们的载体及其用途，特别是用于调节基于蜕皮激素受体的基因表达调节系统中的基因表达的方法和用于鉴定调节的分子的方法 粉虱蜕皮激素受体活性。
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15.发明授权公开(公告)号：US08669051B2
公开(公告)日：2014-03-11
申请号：US11841529
申请日：2007-08-20
IPC分类号： C12Q1/68 , G01N33/567 , C12P21/06 , C12N5/00 , C12N5/07 , C12N5/02 , C12N1/20 , C12N1/38 , C12N15/00 , C07H21/04
CPC分类号： C12N15/635 , C07K14/70567 , C07K14/721 , C12N7/00 , C12N15/86 , C12N2710/10043 , C12P21/02
摘要： This invention relates to the field of biotechnology or genetic engineering. Specifically, this invention relates to the field of gene expression. More specifically, this invention relates to novel substitution mutant receptors and their use in a Group H nuclear receptor-based inducible gene expression system and methods of modulating the expression of a gene in a host cell for applications such as gene therapy, large scale production of proteins and antibodies, cell-based high throughput screening assays, functional genomics and regulation of traits in transgenic organisms.
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16.发明申请公开(公告)号：US20120028300A1
公开(公告)日：2012-02-02
申请号：US13174620
申请日：2011-06-30
CPC分类号： G01N33/566 , C07K14/43577 , G01N2333/723
摘要： The present invention relates to a novel isolated whitefly ecdysone receptor polypeptide. The invention also relates to an isolated nucleic acid encoding the whitefly ecdysone receptor polypeptide, to vectors comprising them and to their uses, in particular in methods for modulating gene expression in an ecdysone receptor-based gene expression modulation system and methods for identifying molecules that modulate whitefly ecdysone receptor activity.
摘要翻译： 本发明涉及一种新型的孤立的粉虱蜕皮激素受体多肽。 本发明还涉及编码粉虱蜕皮激素受体多肽的分离的核酸，包含它们的载体及其用途，特别是用于调节基于蜕皮激素受体的基因表达调节系统中的基因表达的方法和用于鉴定调节的分子的方法 粉虱蜕皮激素受体活性。
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公开(公告)号：US08030067B2
公开(公告)日：2011-10-04
申请号：US12888732
申请日：2010-09-23
CPC分类号： G01N33/566 , C07K14/43577 , G01N2333/723
摘要： The present invention relates to a novel isolated whitefly ecdysone receptor polypeptide. The invention also relates to an isolated nucleic acid encoding the whitefly ecdysone receptor polypeptide, to vectors comprising them and to their uses, in particular in methods for modulating gene expression in an ecdysone receptor-based gene expression modulation system and methods for identifying molecules that modulate whitefly ecdysone receptor activity.
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18.发明申请公开(公告)号：US20110097794A1
公开(公告)日：2011-04-28
申请号：US12888732
申请日：2010-09-23
CPC分类号： G01N33/566 , C07K14/43577 , G01N2333/723
摘要： The present invention relates to a novel isolated whitefly ecdysone receptor polypeptide. The invention also relates to an isolated nucleic acid encoding the whitefly ecdysone receptor polypeptide, to vectors comprising them and to their uses, in particular in methods for modulating gene expression in an ecdysone receptor-based gene expression modulation system and methods for identifying molecules that modulate whitefly ecdysone receptor activity.
摘要翻译： 本发明涉及一种新型的孤立的粉虱蜕皮激素受体多肽。 本发明还涉及编码粉虱蜕皮激素受体多肽的分离的核酸，包含它们的载体及其用途，特别是用于调节基于蜕皮激素受体的基因表达调节系统中的基因表达的方法和用于鉴定调节的分子的方法 粉虱蜕皮激素受体活性。
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公开(公告)号：US20110059530A1
公开(公告)日：2011-03-10
申请号：US12859940
申请日：2010-08-20
CPC分类号： C12N15/63 , C07K2319/71 , C07K2319/715 , C07K2319/80 , C12N15/1055 , C12N15/85 , C12N2510/00 , C12N2830/002 , C12N2830/15
摘要： This invention relates to the field of biotechnology or genetic engineering. Specifically, this invention relates to the field of gene expression. More specifically, this invention relates to a novel inducible gene expression system and methods of modulating gene expression in a host cell for applications such as gene therapy, large scale production of proteins and antibodies, cell-based high throughput screening assays, functional genomics and regulation of traits in transgenic plants and animals.
摘要翻译： 本发明涉及生物技术或遗传工程领域。 具体地，本发明涉及基因表达领域。 更具体地，本发明涉及一种新的诱导型基因表达系统和调节宿主细胞中基因表达的方法，用于诸如基因治疗，蛋白质和抗体的大规模生产，基于细胞的高通量筛选测定，功能基因组学和调节 的转基因植物和动物的性状。
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公开(公告)号：US07829676B2
公开(公告)日：2010-11-09
申请号：US12553560
申请日：2009-09-03
IPC分类号： C07K16/18
CPC分类号： G01N33/566 , C07K14/43577 , G01N2333/723
摘要： The present invention relates to a novel isolated whitefly ecdysone receptor polypeptide. The invention also relates to an isolated nucleic acid encoding the whitefly ecdysone receptor polypeptide, to vectors comprising them and to their uses, in particular in methods for modulating gene expression in an ecdysone receptor-based gene expression modulation system and methods for identifying molecules that modulate whitefly ecdysone receptor activity.
摘要翻译： 本发明涉及一种新型的孤立的粉虱蜕皮激素受体多肽。 本发明还涉及编码粉虱蜕皮激素受体多肽的分离的核酸，包含它们的载体及其用途，特别是用于调节基于蜕皮激素受体的基因表达调节系统中的基因表达的方法和用于鉴定调节的分子的方法 粉虱蜕皮激素受体活性。
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