公开(公告)号：US06916605B1

公开(公告)日：2005-07-12

申请号：US09196522

申请日：1998-11-20

申请人： John McCafferty ,  Anthony Richard Pope ,  Kevin Stuart Johnson ,  Hendricus Renerus Jacobus Mattheus Hoogenboom ,  Andrew David Griffiths ,  Ronald Henry Jackson ,  Kaspar Philipp Holliger ,  James David Marks ,  Timothy Piers Clackson ,  David John Chiswell ,  Gregory Paul Winter ,  Timothy Peter Bonnert

发明人： John McCafferty ,  Anthony Richard Pope ,  Kevin Stuart Johnson ,  Hendricus Renerus Jacobus Mattheus Hoogenboom ,  Andrew David Griffiths ,  Ronald Henry Jackson ,  Kaspar Philipp Holliger ,  James David Marks ,  Timothy Piers Clackson ,  David John Chiswell ,  Gregory Paul Winter ,  Timothy Peter Bonnert

IPC分类号： C07K16/00 ,  C07K16/18 ,  C07K16/34 ,  C07K16/40 ,  C07K16/44 ,  C07K16/46 ,  C07K19/00 ,  C12N15/10 ,  C12N15/62 ,  C12N15/73 ,  C40B40/02 ,  G01N33/53 ,  G01N33/68 ,  C12Q1/70 ,  C07H21/02 ,  C12N15/00

CPC分类号： C40B40/02 ,  B01J2219/00718 ,  C07K16/00 ,  C07K16/18 ,  C07K16/34 ,  C07K16/40 ,  C07K16/44 ,  C07K16/462 ,  C07K16/468 ,  C07K19/00 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/55 ,  C07K2317/622 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/41 ,  C07K2319/735 ,  C12N15/1037 ,  C12N15/62 ,  C12N15/73 ,  G01N33/53 ,  G01N33/6857

摘要： A member of a specific binding pair (sbp) is identified by expressing DNA encoding a genetically diverse population of such sbp members in recombinant host cells in which the sbp members are displayed in functional form at the surface of a secreted recombinant genetic display package (rgdp) containing DNA encoding the sbp member or a polypeptide component thereof, by virtue of the sbp member or a polypeptide component thereof being expressed as a fusion with a capsid component of the rgdp. The displayed sbps may be selected by affinity with a complementary sbp member, and the DNA recovered from selected rgdps for expression of the selected sbp members. Antibody sbp members may be thus obtained, with the different chains thereof expressed, one fused to the capsid component and the other in free form for association with the fusion partner polypeptide. A phagemid may be used as an expression vector, with said capsid fusion helping to package the phagemid DNA. Using this method libraries of DNA encoding respective chains of such multimeric sbp members may be combined, thereby obtaining a much greater genetic diversity in the sbp members than could easily be obtained by conventional methods.

摘要翻译： 特异性结合对（sbp）的成员通过在重组宿主细胞中表达编码这种sbp成员的遗传多样性群体的DNA来鉴定，其中sbp成员以分泌重组遗传显示包（rgdp）的表面以功能形式显示 ），其含有编码sbp成员或其多肽组分的DNA，其中sbp成员或其多肽组分被表达为与rgdp的衣壳组分的融合物。 显示的sbps可以通过与补充sbp成员的亲和力来选择，并且从所选的rgdps中恢复的DNA用于表达所选择的sbp成员。 因此可以获得抗体sbp成员，其中不同的链表达，一个与衣壳组分融合，另一个与游离形式融合，用于与融合伴侣多肽结合。 噬菌粒可以用作表达载体，所述衣壳融合有助于包装噬菌粒DNA。 使用这种方法，可以组合编码这种多聚体sbp成员的各个链的DNA文库，从而获得在sbp成员中比通过常规方法容易地获得的更大的遗传多样性。

公开(公告)号：US5885793A

公开(公告)日：1999-03-23

申请号：US244597

申请日：1994-10-26

申请人： Andrew David Griffiths ,  Hendricus Renerus Jacobus Mattheus Hoogenboom ,  James David Marks ,  John McCafferty ,  Gregory Paul Winter ,  Geoffrey Walter Grigg

发明人： Andrew David Griffiths ,  Hendricus Renerus Jacobus Mattheus Hoogenboom ,  James David Marks ,  John McCafferty ,  Gregory Paul Winter ,  Geoffrey Walter Grigg

IPC分类号： G01N33/531 ,  C07K14/00 ,  C07K16/00 ,  C07K16/18 ,  C07K16/22 ,  C07K16/24 ,  C07K16/26 ,  C07K16/28 ,  C07K16/30 ,  C07K16/34 ,  C07K16/40 ,  C07K16/42 ,  C07K16/46 ,  C07K19/00 ,  C12N7/01 ,  C12N15/09 ,  C12N15/10 ,  C12N15/13 ,  C12N15/62 ,  C12P21/08 ,  C12R1/19 ,  C40B40/02 ,  G01N33/564 ,  G01N33/577 ,  G01N33/68 ,  C07H21/04 ,  C12P21/06

CPC分类号： C40B40/02 ,  C07K16/005 ,  C07K16/18 ,  C07K16/22 ,  C07K16/241 ,  C07K16/26 ,  C07K16/28 ,  C07K16/2812 ,  C07K16/2863 ,  C07K16/2866 ,  C07K16/3007 ,  C07K16/3092 ,  C07K16/34 ,  C07K16/40 ,  C07K16/4208 ,  C07K16/4241 ,  C07K16/44 ,  C07K16/462 ,  C07K16/468 ,  C12N15/1037 ,  C12N15/62 ,  B01J2219/00718 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/55 ,  C07K2317/622 ,  C07K2317/92 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/41 ,  C07K2319/735 ,  Y10S530/867

摘要： Methods are disclosed for the production of anti-self antibodies and antibody fragments, being antibodies or fragments of a particular species of mammal which bind self-antigens of that species. Methods comprise providing a library of replicable genetic display packages (rgdps), such as filamentous phage, each rgdp displaying at its surface a member of a specific binding pair which is an antibody or antibody fragment, and each rgdp containing nucleic acid sequence derived from a species of mammal. The nucleic acid sequence in each rgdp encodes a polypeptide chain which is a component part of the sbp member displayed at the surface of that rgdp. Anti-self antibody fragments are selected by binding with a self antigen from the said species of mammal. The displayed antibody fragments may be scFv, Fd, Fab or any other fragment which has the capability of binding antigen. Nucleic acid libraries used may be derived from a rearranged V-gene sequences of unimmunised mammal. Synthetic or artificial libraries are described and shown to be useful.

摘要翻译： PCT No.PCT / GB92 / 02240 Sec。 371日期：1994年10月26日 102（e）日期1994年10月26日PCT提交1992年12月2日PCT公布。 出版物WO93 / 11236 日期1993年6月10日公开了用于产生抗自身抗体和抗体片段的方法，所述抗自身抗体和抗体片段是结合该物种的自身抗原的特定哺乳动物种类的抗体或片段。 方法包括提供可复制的遗传显示包（rgdps）文库，例如丝状噬菌体，每个rgdp在其表面上显示作为抗体或抗体片段的特异性结合对的成员，以及每个含rgdp的核酸序列衍生自 哺乳动物的种类 每个rgdp中的核酸序列编码多肽链，其是在该rgdp的表面显示的sbp成员的组成部分。 通过与来自所述哺乳动物种的自身抗原结合来选择抗自身抗体片段。 显示的抗体片段可以是scFv，Fd，Fab或具有结合抗原能力的任何其它片段。 所使用的核酸文库可以来源于未免疫的哺乳动物的重新排列的V基因序列。 描述和显示合成或人造文库是有用的。

公开(公告)号：US09475877B2

公开(公告)日：2016-10-25

申请号：US12558032

申请日：2009-09-11

申请人： Els Anna Alice Beirnaert ,  Sigrid Cornelis ,  Hendricus Renerus Jacobus Mattheus Hoogenboom

发明人： Els Anna Alice Beirnaert ,  Sigrid Cornelis ,  Hendricus Renerus Jacobus Mattheus Hoogenboom

IPC分类号： C07K16/18 ,  A61K39/395 ,  C07K16/28 ,  C07K16/24 ,  A61K39/00

CPC分类号： C07K16/2875 ,  A61K39/3955 ,  A61K47/60 ,  A61K47/6849 ,  A61K2039/505 ,  C07K14/765 ,  C07K16/24 ,  C07K2317/22 ,  C07K2317/24 ,  C07K2317/31 ,  C07K2317/35 ,  C07K2317/565 ,  C07K2317/567 ,  C07K2317/569 ,  C07K2317/76 ,  C07K2317/94 ,  C07K2319/31

摘要： The present invention relates to amino acid sequences that are directed against RANK-L, as well as to compounds or constructs, and in particular proteins and polypeptides, that comprise or essentially consist of one or more such amino acid sequences. The invention also relates to nucleic acids encoding such amino acid sequences and polypeptides; to methods for preparing such amino acid sequences and polypeptides; to host cells expressing or capable of expressing such amino acid sequences or polypeptides; to compositions, and in particular to pharmaceutical compositions, that comprise such amino acid sequences, polypeptides, nucleic acids and/or host cells; and to uses of such amino acid sequences or polypeptides, nucleic acids, host cells and/or compositions, in particular for prophylactic, therapeutic or diagnostic purposes.

摘要翻译： 本发明涉及针对RANK-L的氨基酸序列，以及包含或基本上由一个或多个这样的氨基酸序列组成的化合物或构建体，特别是蛋白质和多肽。 本发明还涉及编码这种氨基酸序列和多肽的核酸; 涉及制备这种氨基酸序列和多肽的方法; 宿主表达或能够表达这种氨基酸序列或多肽的细胞; 包括这样的氨基酸序列，多肽，核酸和/或宿主细胞的组合物，特别是药物组合物; 以及这些氨基酸序列或多肽，核酸，宿主细胞和/或组合物的用途，特别是用于预防，治疗或诊断目的。

公开(公告)号：US09040258B2

公开(公告)日：2015-05-26

申请号：US13170362

申请日：2011-06-28

申请人： Simon E. Hufton ,  Hendricus Renerus Jacobus Mattheus Hoogenboom

发明人： Simon E. Hufton ,  Hendricus Renerus Jacobus Mattheus Hoogenboom

IPC分类号： C12P21/00 ,  C40B30/04 ,  C40B40/02 ,  G01N33/554 ,  C12N15/10 ,  C12N15/85

CPC分类号： C12N15/1037 ,  C12N15/85 ,  C12N2795/00043 ,  C12N2800/108 ,  C12N2830/002 ,  C12N2840/20 ,  C40B40/02 ,  G01N33/554

摘要： A eukaryotic expression vector capable of displaying a multi-chain polypeptide on the surface of a host cell is provided, such that the biological activity of the multi-chain polypeptide is exhibited at the surface of the host cell. Such a vector allows for the display of complex biologically active polypeptides, e.g., biologically active multi-chain polypeptides such as immunoglobulin Fab fragments. The present invention describes and enables the successful display of a multi-chain polypeptide on the surface of a eukaryotic host cell. Preferred vectors are described for expressing the chains of a multi-chain polypeptide in a host cell separately and independently (e.g., under separate vector control elements, and/or on separate expression vectors, thus forming a matched vector set). The use of such matched vector sets provides flexibility and versatility in the generation of eukaryotic display libraries, for example the ability to generate and to display multi-chain polypeptides by combining and recombining vectors that express variegations of the individual chains of a multi-chain polypeptide. Entire repertoires of novel chain combinations can be devised using such vector sets.

公开(公告)号：US07919257B2

公开(公告)日：2011-04-05

申请号：US11292414

申请日：2005-11-30

申请人： Hendricus Renerus Jacobus Mattheus Hoogenboom ,  Ton Logtenberg

发明人： Hendricus Renerus Jacobus Mattheus Hoogenboom ,  Ton Logtenberg

IPC分类号： G01N33/53 ,  C12P21/04 ,  C07K16/00 ,  A61K39/00 ,  C12N5/07 ,  C12P21/08 ,  A01N25/00

CPC分类号： G01N33/56966 ,  C07K16/005 ,  C07K16/10 ,  C07K16/22 ,  C07K16/248 ,  C07K16/2863 ,  C07K16/32 ,  C07K2317/14 ,  C07K2317/24 ,  C07K2317/31 ,  C07K2317/34 ,  C07K2317/52 ,  C07K2317/55 ,  C07K2317/56 ,  C07K2317/622 ,  C07K2317/76 ,  C07K2317/94 ,  C07K2319/00

摘要： The present invention provides combinations of specific binding proteins, such as immunoglobulins, that are designed to be true combinations, essentially all components of the combination being functional and compatible with each other. The invention further provides a method for producing a composition comprising at least two different proteinaceous molecules comprising paired variable regions, the at least two proteinaceous molecules having different binding specificities, comprising paired variable regions, at least two proteinaceous molecules having different binding specificities, comprising contacting at least three different variable regions under conditions allowing for pairing of variable regions and harvesting essentially all proteinaceous molecules having binding specificities resulting from the pairing.

摘要翻译： 本发明提供了被设计为真实组合的特异性结合蛋白（例如免疫球蛋白）的组合，该组合的基本上所有组分都是功能性的并且彼此相容。 本发明进一步提供一种制备包含至少两种不同蛋白质分子的组合物的方法，所述蛋白质分子包含成对的可变区，所述至少两种具有不同结合特异性的蛋白质分子包含成对的可变区，至少两种具有不同结合特异性的蛋白质分子，包括接触 至少三个不同的可变区，在允许可变区配对和收获基本上所有具有由配对产生的结合特异性的蛋白质分子的条件下。

公开(公告)号：US20100129368A9

公开(公告)日：2010-05-27

申请号：US11974186

申请日：2007-10-11

申请人： Ignace Joseph Isabella Lasters ,  Hendricus Renerus Jacobus Mattheus Hoogenboom

发明人： Ignace Joseph Isabella Lasters ,  Hendricus Renerus Jacobus Mattheus Hoogenboom

IPC分类号： A61K39/395 ,  C07K14/00 ,  C07K16/18 ,  C12N15/11 ,  A61P43/00 ,  C12N5/06 ,  C12P21/04 ,  A61K38/00

CPC分类号： C07K14/001 ,  A61K38/00 ,  C12N15/1037

摘要： The present invention relates to amino acid sequences that binding to serum proteins such as serum albumin; to compounds, proteins and polypeptides comprising or essentially consisting of such amino acid sequences; to nucleic acids that encode such amino acid sequences, proteins or polypeptides; to compositions, and in particular pharmaceutical compositions, that comprise such amino acid sequences, proteins and polypeptides; and to uses of such amino acid sequences, proteins and polypeptides, is essentially independent in the pH range 5 to 8.

摘要翻译： 本发明涉及与血清蛋白如血清白蛋白结合的氨基酸序列; 包括或基本上由这样的氨基酸序列组成的化合物，蛋白质和多肽; 涉及编码这种氨基酸序列，蛋白质或多肽的核酸; 包括这样的氨基酸序列，蛋白质和多肽的组合物，特别是药物组合物; 并且使用这些氨基酸序列，蛋白质和多肽，在5至8的pH范围内基本上是独立的。

公开(公告)号：US20100120681A1

公开(公告)日：2010-05-13

申请号：US12523256

申请日：2008-02-21

申请人： Pascal Gerard Merchiers ,  Peter Verheesen ,  Hendricus Renerus Jacobus Mattheus Hoogenboom

发明人： Pascal Gerard Merchiers ,  Peter Verheesen ,  Hendricus Renerus Jacobus Mattheus Hoogenboom

IPC分类号： A61K38/16 ,  C07K14/00 ,  C12P21/00 ,  C07H21/00 ,  A61P35/00

CPC分类号： C07K16/22 ,  C07K2317/22 ,  C07K2317/569 ,  C07K2317/73 ,  C07K2317/76 ,  C07K2317/92

摘要： The present invention relates to amino acid sequences that are directed against vascular endothelial growth factor (VEGF), as well as to compounds or constructs, and in particular proteins and polypeptides, that comprise or essentially consist of one or more such amino acid sequences. The amino acid sequences, compounds and constructs can be used for prophylactic, therapeutic or diagnostic purposes, such as for the treatment of conditions and diseases characterized by excessive and/or pathological angiogenesis or neovascularization.

摘要翻译： 本发明涉及针对血管内皮生长因子（VEGF）以及包含或基本上由一个或多个这样的氨基酸序列组成的化合物或构建体，特别是蛋白质和多肽的氨基酸序列。 氨基酸序列，化合物和构建体可用于预防，治疗或诊断目的，例如用于治疗以过度和/或病理性血管生成或新生血管形成为特征的病症和疾病。

公开(公告)号：US20090252681A1

公开(公告)日：2009-10-08

申请号：US12083406

申请日：2006-10-11

申请人： Toon Laeremans ,  Hans De Haard ,  Hendricus Renerus Jacobus Mattheus Hoogenboom

发明人： Toon Laeremans ,  Hans De Haard ,  Hendricus Renerus Jacobus Mattheus Hoogenboom

IPC分类号： A61K39/395 ,  C07K16/28 ,  C07K2/00 ,  C12P21/00 ,  C07H21/04 ,  C12N1/21 ,  C12N5/10 ,  C12N1/15 ,  A61K49/00 ,  A61P35/00 ,  A61P29/00 ,  A61P17/00 ,  G01N33/53

CPC分类号： G01N33/74 ,  C07K16/00 ,  C07K16/18 ,  C07K16/2863 ,  C07K16/3053 ,  C07K2317/22 ,  C07K2317/24 ,  C07K2317/31 ,  C07K2317/55 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/569 ,  C07K2317/73 ,  C07K2317/76 ,  C07K2317/92 ,  C07K2319/30 ,  G01N2333/61 ,  G01N2333/62

摘要： The invention relates to polypeptides and Nanobodies against Epidermal Growth Factor Receptor (EGFR) and/or Insulin Growth Factor-I Receptor (IGF-IR). The invention also relates to nucleic acids encoding such Nanobodies and polypeptides; to methods for preparing such Nanobodies and polypeptides; to host cells expressing or capable of expressing such Nanobodies or polypeptides; to compositions, and in particular to pharmaceutical compositions, that comprise such Nanobodies, polypeptides, nucleic acids and/or host cells; and to uses of such Nanobodies, polypeptides, nucleic acids, host cells and/or compositions, in particular for prophylactic, therapeutic or diagnostic purposes.

摘要翻译： 本发明涉及针对表皮生长因子受体（EGFR）和/或胰岛素生长因子-I受体（IGF-1R）的多肽和纳米抗体。 本发明还涉及编码这种纳米抗体和多肽的核酸; 制备这种纳米抗体和多肽的方法; 宿主表达或能够表达这种纳米抗体或多肽的细胞; 包括这样的纳米抗体，多肽，核酸和/或宿主细胞的组合物，特别是药物组合物; 以及这些纳米抗体，多肽，核酸，宿主细胞和/或组合物的用途，特别是用于预防，治疗或诊断目的。

公开(公告)号：US07195866B2

公开(公告)日：2007-03-27

申请号：US10326495

申请日：2002-12-19

申请人： Andrew David Griffiths ,  Hendricus Renerus Jacobus Mattheus Hoogenboom ,  James David Marks ,  John McCafferty ,  Gregory Paul Winter ,  Geoffrey Walter Grigg

发明人： Andrew David Griffiths ,  Hendricus Renerus Jacobus Mattheus Hoogenboom ,  James David Marks ,  John McCafferty ,  Gregory Paul Winter ,  Geoffrey Walter Grigg

IPC分类号： C12N15/00 ,  C12P21/06 ,  C12P21/08 ,  C12Q1/70 ,  G01N33/53

CPC分类号： C40B40/02 ,  B01J2219/00718 ,  C07K16/005 ,  C07K16/18 ,  C07K16/22 ,  C07K16/241 ,  C07K16/26 ,  C07K16/28 ,  C07K16/2812 ,  C07K16/2863 ,  C07K16/2866 ,  C07K16/3007 ,  C07K16/3092 ,  C07K16/34 ,  C07K16/40 ,  C07K16/4208 ,  C07K16/4241 ,  C07K16/44 ,  C07K16/462 ,  C07K16/468 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/55 ,  C07K2317/622 ,  C07K2317/92 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/41 ,  C07K2319/735 ,  C12N15/1037 ,  C12N15/62 ,  Y10S530/867

摘要： Methods are disclosed for the production of anti-self antibodies and antibody fragments, being antibodies or fragments of a particular species of mammal which bind self antigens of that species. Methods comprise providing a library of replicable genetic display packages (rgdps), such as filamentous phage, each rgdp displaying at its surface member of a specific binding pair which is an antibody or antibody fragment, and each rgdp containing nucleic acid sequence derived from a species of mammal. The nucleic acid sequence in each rgdp encodes a polypeptide chain which is a component part of the sbp member displayed at the surface of that rgdp. Anti-self antibody fragments are selected by binding with a self antigen from the said species of mammal. The displayed antibody fragments may be scFv, Fd, Fab or any other fragment which has the capability of binding antigen. Nucleic acid libraries used may be derived from a rearranged V-gene sequences of unimmunised mammal. Synthetic or artificial libraries are described and shown to be useful.

摘要翻译： 公开了用于产生抗自身抗体和抗体片段的方法，其是结合该物种的自身抗原的特定哺乳动物种类的抗体或片段。 方法包括提供可复制的遗传显示包（rgdps）文库，例如丝状噬菌体，每个rgdp在其作为抗体或抗体片段的特异性结合对的表面成员显示，并且每个含有rgdp的核酸序列衍生自物种 的哺乳动物。 每个rgdp中的核酸序列编码多肽链，其是在该rgdp的表面显示的sbp成员的组成部分。 通过与来自所述哺乳动物种的自身抗原结合来选择抗自身抗体片段。 显示的抗体片段可以是scFv，Fd，Fab或具有结合抗原能力的任何其它片段。 所使用的核酸文库可以来源于未免疫的哺乳动物的重新排列的V基因序列。 描述和显示合成或人造文库是有用的。

公开(公告)号：US06555313B1

公开(公告)日：2003-04-29

申请号：US09572392

申请日：2000-05-16

申请人： Andrew David Griffiths ,  Hendricus Renerus Jacobus Mattheus Hoogenboom ,  James David Marks ,  John McCafferty ,  Gregory Paul Winter ,  Geoffrey Walter Grigg

发明人： Andrew David Griffiths ,  Hendricus Renerus Jacobus Mattheus Hoogenboom ,  James David Marks ,  John McCafferty ,  Gregory Paul Winter ,  Geoffrey Walter Grigg

IPC分类号： C12Q170

CPC分类号： C40B40/02 ,  B01J2219/00718 ,  C07K16/005 ,  C07K16/18 ,  C07K16/22 ,  C07K16/241 ,  C07K16/26 ,  C07K16/28 ,  C07K16/2812 ,  C07K16/2863 ,  C07K16/2866 ,  C07K16/3007 ,  C07K16/3092 ,  C07K16/34 ,  C07K16/40 ,  C07K16/4208 ,  C07K16/4241 ,  C07K16/44 ,  C07K16/462 ,  C07K16/468 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/55 ,  C07K2317/622 ,  C07K2317/92 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/41 ,  C07K2319/735 ,  C12N15/1037 ,  C12N15/62 ,  Y10S530/867

摘要： Methods are disclosed for the production of human self-antibodies and antibody fragments, which bind human antigens. Methods comprise providing a library of replicable genetic display packages (rgdps), such as filamentous phage, each rgdp displaying at its surface a member of a specific binding pair which is an antibody or antibody fragment, and each rgdp containing nucleic acid sequence derived from a species of mammal. The nucleic acid sequence in each rgdp encodes a polypeptide chain which is a component part of the sbp member displayed at the surface of that rgdp. Human antibodies or antibody fragments are selected by binding with human antigens. The displayed antibody fragments may be scFv, Fd, Fab or any other fragment which has the capability of binding to and is a human antigen. Nucleic acid libraries used may be derived from V-gene sequences of unimmunised humans. Part or all of the nucleic acid may be derived from oligonucleotide synthesis.

摘要翻译： 公开了用于生产结合人抗原的人自身抗体和抗体片段的方法。 方法包括提供可复制的遗传显示包（rgdps）文库，例如丝状噬菌体，每个rgdp在其表面上显示作为抗体或抗体片段的特异性结合对的成员，以及每个含rgdp的核酸序列衍生自 哺乳动物的种类 每个rgdp中的核酸序列编码多肽链，其是在该rgdp的表面显示的sbp成员的组成部分。 通过与人抗原结合来选择人抗体或抗体片段。 显示的抗体片段可以是scFv，Fd，Fab或具有结合并且是人抗原能力的任何其它片段。 所用的核酸文库可以衍生自未免疫的人的V-基因序列。 部分或全部核酸可以衍生自寡核苷酸合成。