公开(公告)号：US20250019752A1

公开(公告)日：2025-01-16

申请号：US18706621

申请日：2022-11-02

Applicant: Life Technologies Corporation

Inventor： Daniel Philippe Blanchard ,  Maria Cinta Gomez Silvan

IPC: C12Q1/6848 ,  C12Q1/686

Abstract: This disclosure describes master mix compositions, nucleic acid amplification kits, methods of manufacturing master mix compositions, and methods of using master mix compositions that minimize or eliminate the negative effects of contaminant nucleic acids. Conventional master mix compositions often include contaminant nucleic acids.

公开(公告)号：US20250011863A1

公开(公告)日：2025-01-09

申请号：US18769897

申请日：2024-07-11

Applicant: Life Technologies Corporation

Inventor： Dumitru Brinza ,  Zheng Zhang ,  Fiona Hyland ,  Rajesh Gottimukkala

IPC: C12Q1/6874 ,  G16B5/00 ,  G16B5/20 ,  G16B30/00 ,  G16B30/10 ,  G16B30/20

Abstract: Systems and method for determining variants can receive mapped reads, align flow space information to a flow space representation of a corresponding portion of the reference. Reads spanning a position with a potential variant can be evaluated in a context specific manner. A list of probable variants can be provided.

公开(公告)号：US12188000B2

公开(公告)日：2025-01-07

申请号：US18317036

申请日：2023-05-12

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Nephi D. Jones ,  Christopher D. Brau

IPC: B01D46/00 ,  B01D5/00 ,  B01D19/00 ,  B01D46/44 ,  B01D46/58 ,  B01D53/26 ,  C12M1/00 ,  C12M1/34 ,  C12M1/36

Abstract: A method for filtering a gas includes delivering a gas into a compartment of a gas filter assembly; applying a partial vacuum to the gas filter assembly so that the partial vacuum assists in drawing the gas through a porous filter body of the gas filter assembly that is at least partially disposed within the compartment of the gas filter assembly; and regulating the application of the partial vacuum based on a pressure reading of the gas upstream or downstream of the gas filter assembly.

公开(公告)号：US20240426775A1

公开(公告)日：2024-12-26

申请号：US18732345

申请日：2024-06-03

Applicant: Life Technologies Corporation

Inventor： James BUSTILLO ,  Wolfgang HINZ ,  Kim JOHNSON ,  Jonathan ROTHBERG

IPC: G01N27/414 ,  C12Q1/6818 ,  C12Q1/6874 ,  G01N33/543 ,  H01L27/088 ,  H01L29/423 ,  H01L29/78

Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis.

公开(公告)号：USD1053799S1

公开(公告)日：2024-12-10

申请号：US29738833

申请日：2020-06-19

Applicant: LIFE TECHNOLOGIES CORPORATION ,  THERMO FISHER SCIENTIFIC (SHANGHAI) INSTRUMENTS CO. LTD.

Designer： Xin Mathers ,  Guomin (Jimmy) Zheng

公开(公告)号：US20240392226A1

公开(公告)日：2024-11-28

申请号：US18248428

申请日：2021-10-12

Applicant: LIFE TECHNOLOGIES AS ,  LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES HOLDINGS PTE LIMITED

Inventor： Morten LUHR ,  Michael GORDON ,  Ming Tiong SIA ,  Kok Shyong CHONG ,  Woon Liang Terence SOH ,  Seng Leong LIM ,  Ida Caroline SCHROEDER ,  Katrine ALFSNES ,  Terry JianHui LIM ,  Marie BOSNES ,  Axl Alois NEURAUTER ,  Ketil Winther PEDERSEN ,  Autumn Day WILBUR ,  Sean ZIMMERMAN

IPC: C12M1/12 ,  C12M1/00 ,  G01N35/00

Abstract: A bead processing assembly for use in attaching magnetic beads to biological cells and other biological materials and/or separating magnetic beads from biological cells and other biological materials includes a base assembly having a housing, a support panel disposed on the housing and having a front face, a first pinch valve at least partially outwardly projecting from the front face of the support panel, and a first pump at least partially outwardly projecting from the front face of the support panel. A rocker assembly is supported on the base assembly and includes a mount assembly supported on the base assembly, a platform assembly pivotably secured to the mount assembly, and a rocker drive for repeatedly rocking the platform assembly relative to the mount assembly.

公开(公告)号：US12146189B2

公开(公告)日：2024-11-19

申请号：US16433380

申请日：2019-06-06

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Earl Hubbell

IPC: C40B50/16 ,  C12Q1/6869 ,  C12Q1/6874 ,  C40B20/04 ,  C40B70/00 ,  G16B30/00 ,  G16C20/64 ,  G16C99/00

Abstract: A method for sequencing a polynucleotide sample having a barcode sequence includes: introducing a series of nucleotides to the polynucleotide sample according to a predetermined order of nucleotide flows; obtaining a series of signals resulting from the introducing of nucleotides to the polynucleotide sample; and resolving the series of signals over the barcode sequence to render a flowspace string, wherein the flowspace string is a codeword of an error-correcting code that is (i) designed based on and adapted for use with the predetermined order of nucleotide flows, and (ii) capable of distinguishing any codeword in the error-correcting code from the other codewords in the error-correcting code in the presence of zero, one, and two errors.

公开(公告)号：US20240376434A1

公开(公告)日：2024-11-14

申请号：US18690262

申请日：2022-09-16

Applicant: LIFE TECHNOLOGIES CORPORATION ,  NUNC A/S

Inventor： Debra JOHNSON ,  Sune SPORRING

IPC: C12N5/0775 ,  A61K35/28 ,  C12N9/22

Abstract: The instant technology generally relates to methods and compositions for expansion of mesenchymal stem cells in culture on a modified surface in the absence of a cell feeder layer and the absence of a cell adhesive coating on the surface. In some instances, the mesenchymal stem cells are expanded on the modified surface in a serum-free culture medium.

公开(公告)号：US20240376418A1

公开(公告)日：2024-11-14

申请号：US18780337

申请日：2024-07-22

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Nephi D. Jones ,  Christopher D. Brau

IPC: C12M1/06 ,  B01F27/1111 ,  B01F27/191 ,  B01F27/2111 ,  B01F35/41 ,  B01F35/513 ,  B01F101/44 ,  C12M1/00 ,  C12M1/02 ,  C12N1/00

Abstract: A fluid mixing system includes a collapsible container bounding a compartment and extending between a first end and an opposing second end. An elongated continuous drive line is at least partially disposed within the compartment of the container. A first portion of the drive line is laterally spaced apart from a second portion of the drive line, and the first portion of the drive line and the second portion of the drive line are rotatable within the compartment of the container.

公开(公告)号：US20240361348A1

公开(公告)日：2024-10-31

申请号：US18640345

申请日：2024-04-19

Applicant: Life Technologies Corporation

Inventor： Alexandre VLASSOV ,  Timothy BARTA ,  Justin INSLEE ,  Ethan GENTERT ,  Robert SMITH ,  Sara THIELE ,  Carolyn ADAMS ,  Kraig KRUGER ,  Robert Schneider ,  Wade LUKIANOW ,  Jay GRECO ,  Jason JONES ,  David CERRONE ,  Sumonto GHOSH ,  Brian VAJDA ,  Andrew BASTA ,  Allan EVANS ,  Christopher CROWLEY ,  Robert SETTERQUIST ,  Anne COX ,  Mark MAYERNICK

IPC: G01N35/10 ,  B01D61/58 ,  B01D69/02 ,  G01N1/40 ,  G01N21/59 ,  G01N35/00

CPC classification number: G01N35/1016 ,  B01D61/58 ,  B01D69/02 ,  G01N1/405 ,  G01N1/4077 ,  G01N21/59 ,  G01N35/00584 ,  G01N35/1081 ,  B01D2311/2642 ,  B01D2313/44 ,  B01D2313/50 ,  B01D2313/58 ,  B01D2319/025 ,  B01D2325/42 ,  G01N2001/4088 ,  G01N2035/1025 ,  G01N2035/1053

Abstract: Purifying target biomolecules, such as nucleic acids or proteins, from a biological source is a time intensive process and is typically performed by a skilled technician or scientist owing to the highly technical nature of the work. Systems, devices, and methods disclosed herein enable the automated bioprocessing and purification of target biomolecules from a biological source. For example, an instrument and disposable cartridge are provided for automatedly isolating and purifying nucleic acids (such as plasmid DNA from a bacterial culture) or for isolating protein from any biological sample. Such an exemplary instrument and cartridge can work in concert to timely release, mix, and move the target biomolecule and various reagents and buffers through a target biomolecule purification process, resulting in a purified target biomolecule with less manual oversight than traditional approaches.