公开(公告)号：US20100107342A1

公开(公告)日：2010-05-06

申请号：US12567302

申请日：2009-09-25

申请人： MARTIN SCHULEIN ,  TORBEN HENRIKSEN ,  LENE NONBOE ANDERSEN ,  SOREN FLENSTED LASSEN ,  MARKUS SAKARI KAUPPINEN ,  LENE LANGE ,  RUBY IIUM NIELSEN ,  SHINOBU TAKAGI ,  MICHIKO IHARA

发明人： MARTIN SCHULEIN ,  TORBEN HENRIKSEN ,  LENE NONBOE ANDERSEN ,  SOREN FLENSTED LASSEN ,  MARKUS SAKARI KAUPPINEN ,  LENE LANGE ,  RUBY IIUM NIELSEN ,  SHINOBU TAKAGI ,  MICHIKO IHARA

IPC分类号： C11D7/42 ,  C12N9/42

CPC分类号： C12N9/2437 ,  A61K38/00 ,  C05F11/08 ,  C05F17/0036 ,  C07K2319/00 ,  C11D3/386 ,  C12P19/14 ,  C12Y302/01004 ,  D06M16/003 ,  D06P5/02 ,  D06P5/158 ,  D21C5/005 ,  D21H17/005 ,  D21H21/10 ,  Y02P20/145 ,  Y02W30/43

摘要： The present invention relates to enzyme preparations consisting essentially of an enzyme which has cellulytic activity and comprises a first amino acid sequence having the following sequence (SEQ ID NO: 79) Thr Arg Xaa Xaa Asp Cys Cys Xaa Xaa Xaa Cys Xaa 1   2   3   4   5   6   7   8   9   10  11  12 Trp Xaa 13  14 and a second amino acid sequence having the following sequence Trp Cys Cys Xaa Cys(SEQ ID NO: 80) 1   2   3   4   5 wherein, at position 3 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 4 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 8 of the first sequence, the amino acid is Arg, Lys or His; at positions 9, 10, 12 and 14, respectively, of the first sequence, and at position 4 of the second sequence, the amino acid is any of the 20 naturally occurring amino acid residues with the provisos that, in the first amino acid sequence, (i) when the amino residue at position 12 is Ser, then the amino acid residue at position 14 is not Ser, and (ii) when the amino residue at position 12 is Gly, then the amino acid residue at position 14 is not Ala, performs very well in industrial applications such as laundry compositions, for biopolishing of newly manufactured textiles, for providing an abraded look of cellulosic fabric or garment, and for treatment of paper pulp. Further, the invention relates to DNA constructs encoding such enzymes, a method for providing a gene encoding for such enzymes, a method of producing the enzymes, enzyme preparations containing such enzymes, and the use of these enzymes for a number of industrial applications.

公开(公告)号：US07597987B2

公开(公告)日：2009-10-06

申请号：US10525234

申请日：2003-08-19

申请人： Shinobu Takagi ,  Masaki Shinkawa ,  Mikio Ura ,  Shinichi Yagi ,  Yasushi Kaneta ,  Tatsuo Hisada

发明人： Shinobu Takagi ,  Masaki Shinkawa ,  Mikio Ura ,  Shinichi Yagi ,  Yasushi Kaneta ,  Tatsuo Hisada

IPC分类号： H01M8/02 ,  B32B15/01 ,  B32B15/18

CPC分类号： H01M8/0228 ,  H01M8/0206 ,  H01M8/021 ,  H01M8/0254 ,  H01M2008/1095 ,  Y02P70/56 ,  Y10T428/12875 ,  Y10T428/12889 ,  Y10T428/12896 ,  Y10T428/12944 ,  Y10T428/12951 ,  Y10T428/12993

摘要： An Au plated film 12 is formed on the surface of a plate-formed metal base 13 composed of a metal less noble than Au, and the product is cut along a planned cutting line 18 reflecting a contour of a desired component, to thereby form a separator 10. Thus-formed separator 10 has the Au plated film 12 formed on the main surface 10a thereof, and has a cutting plane 16 formed as an end face 16 stretched up to the main surface 10a. The metal base 13 exposes in a part of the cutting plane 16, in a width of the exposed region of 1 mm or less. This is successful in providing a metal component for fuel cell which is satisfactory in the corrosion resistance and allows easy fabrication at low costs, a method of manufacturing the same, and also in providing a fuel cell having thus-fabricated metal component for fuel cell.

摘要翻译： 在由金属低于金属的金属构成的板状金属基体13的表面上形成Au镀膜12，并且沿着反映期望成分的轮廓的规划切割线18切割产品，从而形成 这样形成的隔离物10具有形成在其主表面10a上的Au镀膜12，并且具有形成为延伸到主表面10a的端面16的切割平面16。 金属基底13在切割平面16的一部分中暴露在1mm或更小的暴露区域的宽度上。 这是成功地提供了用于燃料电池的金属部件，其耐腐蚀性良好并且容易以低成本制造，其制造方法以及提供具有如此制造的用于燃料电池的金属组分的燃料电池。

公开(公告)号：US20060154842A1

公开(公告)日：2006-07-13

申请号：US10473533

申请日：2002-04-18

申请人： Akiko Sugio ,  Shinobu Takagi

发明人： Akiko Sugio ,  Shinobu Takagi

IPC分类号： C11D3/386 ,  A21D8/02 ,  C07H21/04 ,  C12P21/06 ,  C12N9/02

CPC分类号： C12P7/6427 ,  A21D8/042 ,  A23L33/195 ,  C11D3/386 ,  C12N9/0069 ,  C12Y113/11012

摘要： The inventors have found a novel fungal lipoxygenase from Magnaporthe salvinii and determined its sequence. They have sequenced the gene and cloned it into E. coli and deposited the clone. Oligonucleotide probes based on the sequence information are useful for screening a eukaryotic library to obtain a lipoxygenase. The lipoxygenase is useful in baking and in a detergent.

摘要翻译： 本发明人发现了一种来自Magnaporthe salvinii的新型真菌脂氧合酶，并确定了其序列。 他们对基因进行了测序，并将其克隆到大肠杆菌中并保藏了该克隆。 基于序列信息的寡核苷酸探针可用于筛选真核文库以获得脂氧合酶。 脂氧合酶可用于烘烤和洗涤剂中。

公开(公告)号：US5929017A

公开(公告)日：1999-07-27

申请号：US809440

申请日：1997-04-24

申请人： Erik Gormsen ,  Naoko Ikegami ,  Masanobu Abo ,  Shinobu Takagi ,  Noriko Tsutsumi ,  Torben Halkier

发明人： Erik Gormsen ,  Naoko Ikegami ,  Masanobu Abo ,  Shinobu Takagi ,  Noriko Tsutsumi ,  Torben Halkier

IPC分类号： C11D3/386 ,  C12N9/20 ,  C12R1/65

CPC分类号： C11D3/38627 ,  C12N9/20 ,  Y10S435/912

摘要： A lipolytic enzyme with high activity at alkaline pH in the absence of Ca.sup.++ can be obtained from strains of filamentous fungi belonging to the genus Absidia. The lipolytic enzymes are effective for improving the effect of detergents towards fatty soiling.

摘要翻译： PCT No.PCT / DK95 / 00424 Sec。 371日期1997年04月24日 102（e）日期1997年4月24日PCT 1995年10月26日PCT PCT。 公开号WO96 / 13578 日期1996年5月9日在不含Ca ++的碱性pH下具有高活性的脂解酶可以从属于the属的丝状真菌菌株获得。 脂肪分解酶对于改善洗涤剂对脂肪污染的影响是有效的。

公开(公告)号：US09222095B2

公开(公告)日：2015-12-29

申请号：US13704856

申请日：2011-06-23

申请人： Hiroaki Udagawa ,  Rikako Taira ,  Shinobu Takagi

发明人： Hiroaki Udagawa ,  Rikako Taira ,  Shinobu Takagi

IPC分类号： C12N15/80 ,  C07K14/195

CPC分类号： C12N15/80 ,  C07K14/195

摘要： The present invention relates to isolated polynucleotides having leader sequence function. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods using the polynucleotides for production of polypeptides.

摘要翻译： 本发明涉及具有前导序列功能的分离的多核苷酸。 本发明还涉及包含多核苷酸的核酸构建体，载体和宿主细胞以及使用多核苷酸产生多肽的方法。

公开(公告)号：US20150097643A1

公开(公告)日：2015-04-09

申请号：US14502056

申请日：2014-09-30

申请人： Shinobu TAKAGI

发明人： Shinobu TAKAGI

IPC分类号： H01F1/01 ,  H01F7/02 ,  H01F41/02 ,  C22C38/00 ,  B23K20/02

CPC分类号： H01F1/01 ,  B23K20/02 ,  B23K20/026 ,  B23K20/16 ,  B23K20/233 ,  B23K2101/06 ,  B23K2103/08 ,  B23K2103/50 ,  C22C38/002 ,  C22C38/005 ,  C22C2202/02 ,  H01F1/0576 ,  H01F1/0577 ,  H01F7/02 ,  H01F7/021 ,  H01F41/0293

摘要： Provided is a combined type RFeB-based magnet, including: a first unit magnet; a second unit magnet; and an interface material that bonds the first unit magnet and the second unit magnet, in which the first unit magnet and the second unit magnet are RFeB-based magnets containing a light rare earth element RL that is at least one element selected from the group consisting of Nd and Pr, Fe, and B, in which the interface material contains at least one compound selected from the group consisting of a carbide, a hydroxide, and an oxide of the light rare earth element RL, and in which an amount of a heavy rare earth element RH that is at least one element selected from the group consisting of Dy, Tb and Ho in the second unit magnet is more than that in the first unit magnet.

摘要翻译： 提供了一种组合型RFeB基磁体，包括：第一单元磁体; 第二单位磁体; 以及键合第一单位磁体和第二单位磁体的界面材料，其中第一单位磁体和第二单位磁体是包含轻稀土元素RL的RFeB基磁体，其是选自以下的至少一种元素： 的Nd和Pr，Fe和B，其中界面材料含有至少一种选自轻稀土元素RL的碳化物，氢氧化物和氧化物的化合物，其中一定量的 在第二单位磁体中，选自Dy，Tb和Ho中的至少一种元素的重稀土元素RH大于第一单位磁体中的重稀土元素RH。

公开(公告)号：US20140017749A1

公开(公告)日：2014-01-16

申请号：US13994310

申请日：2011-12-21

申请人： Randy Deinhammer ,  Suzanne Clark ,  Mauricio Quiros ,  John Matthews ,  Anne Glud Hjulmand ,  Chee Leong Soong ,  Tomoko Matsui ,  Shinobu Takagi

发明人： Randy Deinhammer ,  Suzanne Clark ,  Mauricio Quiros ,  John Matthews ,  Anne Glud Hjulmand ,  Chee Leong Soong ,  Tomoko Matsui ,  Shinobu Takagi

IPC分类号： C12P7/06

CPC分类号： C12P7/06 ,  C12P19/02 ,  C12P19/14 ,  C12P19/20 ,  C12Y302/01001 ,  C12Y302/01003 ,  C12Y304/24 ,  Y02E50/17

摘要： The present invention relates to processes for producing fermentation products from starch-containing material, wherein a thermostable alpha-amylase and optionally a thermostable protease are present and/or added during liquefaction. The invention also relates to a composition suitable for use in a process of the invention.

摘要翻译： 本发明涉及用于从含淀粉材料生产发酵产物的方法，其中在液化期间存在和/或加入热稳定性α-淀粉酶和任选的热稳定蛋白酶。 本发明还涉及适用于本发明方法的组合物。

公开(公告)号：US08263376B2

公开(公告)日：2012-09-11

申请号：US13076803

申请日：2011-03-31

申请人： Akiko Sugio ,  Shinobu Takagi

发明人： Akiko Sugio ,  Shinobu Takagi

IPC分类号： C12N9/02 ,  C12P2/06 ,  C11D3/386

CPC分类号： C12P7/6427 ,  A21D8/042 ,  A23L33/195 ,  C11D3/386 ,  C12N9/0069 ,  C12Y113/11012

摘要： The present disclosure relates to an isolated lipoxygenase. The lipoxygenase of the present disclosure includes polypeptide with at least 80% sequence homology to the mature peptide shown in SEQ ID NO: 2. Lipoxygenase of the present disclosure is suitable for use in, among other things, baking and in detergent compositions.

摘要翻译： 本公开涉及分离的脂氧合酶。 本公开的脂氧合酶包括与SEQ ID NO：2所示的成熟肽具有至少80％的序列同源性的多肽。本公开的脂氧合酶适合用于烘烤和洗涤剂组合物中。

公开(公告)号：US07883883B2

公开(公告)日：2011-02-08

申请号：US10877849

申请日：2004-06-25

申请人： Hiroaki Udagawa ,  Rikako Taira ,  Shinobu Takagi ,  Carsten Hjort ,  Anders Vikso-Nielsen ,  Eric Allain ,  Shiro Fukuyama ,  Tomoko Matsui

发明人： Hiroaki Udagawa ,  Rikako Taira ,  Shinobu Takagi ,  Carsten Hjort ,  Anders Vikso-Nielsen ,  Eric Allain ,  Shiro Fukuyama ,  Tomoko Matsui

IPC分类号： C12N9/24 ,  C12N9/30 ,  C07H21/04

CPC分类号： C12P19/14 ,  C07K2319/00 ,  C12N9/2408 ,  C12N9/2411 ,  C12N9/2414 ,  C12N9/242 ,  C12N9/2425 ,  C12N9/2428 ,  C12P7/06 ,  C12P19/02 ,  C12P19/16 ,  C12P19/22 ,  C13K11/00 ,  Y02E50/17

摘要： The present invention relates to a hybrid enzyme comprising carbohydrate-binding module amino acid sequence and a fungal alpha-amylase amino acid sequence and to a variant of a fungal wild type enzyme comprising a carbohydrate-binding module and an alpha-amylase catalytic module. The invention also relates to the use of the hybrid enzyme or the variant in starch liquefaction.

摘要翻译： 本发明涉及包含碳水化合物结合模块氨基酸序列和真菌α-淀粉酶氨基酸序列以及包含碳水化合物结合模块和α-淀粉酶催化模块的真菌野生型酶的变体的杂合酶。 本发明还涉及混合酶或变体在淀粉液化中的用途。

公开(公告)号：US20070292581A1

公开(公告)日：2007-12-20

申请号：US11832472

申请日：2007-08-01

申请人： Akiko Sugio ,  Shinobu Takagi

发明人： Akiko Sugio ,  Shinobu Takagi

IPC分类号： A23L1/277 ,  A21D2/08 ,  C07H21/04 ,  C09B67/00 ,  C12N1/15 ,  C12N15/00 ,  C12N9/02 ,  C12P21/00 ,  C12P7/64

CPC分类号： C12P7/6427 ,  A21D8/042 ,  A23L33/195 ,  C11D3/386 ,  C12N9/0069 ,  C12Y113/11012

摘要： The inventors have found a novel fungal lipoxygenase from Manaporthe salvinii and determined its sequence. They have sequenced the gene and cloned it into E. coli and deposited the clone. Oligonucleotides probes based on the sequence information are useful for screening a eukaryotic library to obtain a lipoxygenase. The lipoxygenase is useful in baking and in a detergent.

摘要翻译： 本发明人已经发现了一种来自Manaporthe salvinii的新型真菌脂氧合酶并确定其序列。 他们对基因进行了测序，并将其克隆到大肠杆菌中并保藏了该克隆。 基于序列信息的寡核苷酸探针可用于筛选真核文库以获得脂氧合酶。 脂氧合酶可用于烘烤和洗涤剂中。