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    Probe composition and method
    14.
    发明申请
    Probe composition and method 失效
    探头组成和方法

    公开(公告)号：US20050112609A1

    公开(公告)日：2005-05-26

    申请号：US10825624

    申请日：2004-04-14

    Applicant: Paul Grossman ,  Steven Fung ,  Steven Menchen ,  Sam Woo ,  Emily Winn-Deen

    Inventor： Paul Grossman ,  Steven Fung ,  Steven Menchen ,  Sam Woo ,  Emily Winn-Deen

    IPC: C12Q1/68

    CPC classification number: C12Q1/6816 ,  C12Q1/6825 ,  C12Q1/6827 ,  C12Q1/686 ,  C12Q1/6862 ,  C12Q2600/156 ,  G01N27/447 ,  Y10T436/143333 ,  C12Q2525/197 ,  C12Q2525/203 ,  C12Q2537/143 ,  C12Q2565/131 ,  C12Q2565/125 ,  C12Q2565/107 ,  C12Q2521/501 ,  C12Q2563/155 ,  C12Q2563/119 ,  C12Q2549/119

    Abstract: Method and composition for detecting one or more selected polynucleotide regions in a target polynucleotide. In the method, a mixture of sequence-specific probes are reacted with the target polynucleotide under hybridization conditions, and the hybridized probes are treated to selectively modify those probes which are bound to the target polynucleotide in a base-specific manner. The resulting labeled probes include a polymer chain which imparts to each different-sequence probe, a distinctive ratio of charge/translational frictional drag, and a detectable label. The labeled probes are fractionated by electrophoresis in a non-sieving matrix, and the presence of one or more selected sequences in the target polynucleotide are detected according to the observed electrophoretic migration rates of the labeled probes in a non-sieving medium.

    Abstract translation: 用于检测靶多核苷酸中的一个或多个选择的多核苷酸区域的方法和组合物。 在该方法中，将序列特异性探针的混合物与靶多核苷酸在杂交条件下反应，并对杂交的探针进行处理以选择性地修饰以碱基特异性方式结合靶多核苷酸的那些探针。 所得到的标记探针包括赋予每个不同序列探针的聚合物链，电荷/平移摩擦阻力的独特比例和可检测标记。 标记的探针通过在非筛选基质中的电泳分级分离，并且根据在非筛选培养基中标记的探针的观察到的电泳迁移速率来检测靶多核苷酸中一个或多个选定序列的存在。

    Unnatural polymerase substrates that can sustain enzymatic synthesis of double stranded nucleic acids from a nucleic acid template and methods of use
    15.
    发明授权
    Unnatural polymerase substrates that can sustain enzymatic synthesis of double stranded nucleic acids from a nucleic acid template and methods of use 有权
    可以维持来自核酸模板的双链核酸酶的合成和使用方法的非天然聚合酶底物

    公开(公告)号：US08058414B2

    公开(公告)日：2011-11-15

    申请号：US12355487

    申请日：2009-01-16

    Applicant: Steven Menchen ,  Barnett Rosenblum ,  Paul Kenney ,  Shoeb Khan ,  Zhaochun Ma ,  Jer-Kang Chen ,  Joe Y. Lam ,  Boli Huang

    Inventor： Steven Menchen ,  Barnett Rosenblum ,  Paul Kenney ,  Shoeb Khan ,  Zhaochun Ma ,  Jer-Kang Chen ,  Joe Y. Lam ,  Boli Huang

    IPC: C07G3/00 ,  C07H21/02 ,  C07H21/04 ,  C07H19/04 ,  C12Q1/68 ,  C12P19/34

    CPC classification number: C07H21/04 ,  C07H19/073 ,  C07H19/173 ,  C12Q1/6874 ,  C12Q2523/107 ,  C12Q2525/186 ,  C12Q2525/197

    Abstract: Nucleotide analogs that can sustain the enzymatic synthesis of double-stranded nucleic acid from a nucleic template are described. The nucleotide analogs include: (i) a base selected from the group consisting of adenine, guanine, cytosine, thymine, uracil and their analogs; (ii) a label attached to the base or analog of the base via a cleavable linker; (iii) a deoxyribose; and (iv) one or more phosphate groups. The linker and/or the label inhibits template directed polymerase incorporation of a further nucleotide substrate onto an extended primer strand. In addition, cleavage of the linker leaves a residue attached to the base which is not present in the natural nucleotide and which does not inhibit extension of the primer strand. The nucleotide analogs can therefore be used as reversible terminators in sequencing by synthesis methods without blocking the 3′ hydroxyl group. Methods of sequencing DNA using the substrates are also described.

    Abstract translation: 描述了可以维持来自核酸模板的双链核酸的酶合成的核苷酸类似物。 核苷酸类似物包括：（i）选自腺嘌呤，鸟嘌呤，胞嘧啶，胸腺嘧啶，尿嘧啶及其类似物的碱基; （ii）通过可切割连接体连接到碱基或类似物的标签; （iii）脱氧核糖; 和（iv）一个或多个磷酸酯基团。 接头和/或标记物将另外的核苷酸底物的模板定向聚合酶掺入到延伸的引物链上。 此外，接头的切割物留下连接到碱基上的残基，其不存在于天然核苷酸中，并且不抑制引物链的延伸。 因此，核苷酸类似物因此可以通过合成方法进行测序而不会阻断3'羟基作为可逆终止子。 还描述了使用底物测序DNA的方法。

    Polymers for separation of biomolecules by capillary electrophoresis
    18.
    发明申请
    Polymers for separation of biomolecules by capillary electrophoresis 有权

    公开(公告)号：US20060137982A1

    公开(公告)日：2006-06-29

    申请号：US11359713

    申请日：2006-02-21

    Applicant: Ramakrishna Madabhushi ,  Steven Menchen ,  J. Efcavitch ,  Paul Grossman

    Inventor： Ramakrishna Madabhushi ,  Steven Menchen ,  J. Efcavitch ,  Paul Grossman

    IPC: B01D57/02

    CPC classification number: G01N27/44752 ,  G01N27/44747

    Abstract: The invention provides uncharged water-soluble silica-adsorbing polymers for suppressing electroendoosmotic flow and to reduce analyte-wall interactions in capillary electrophoresis. In one aspect of the invention, one or more of such polymers are employed as components of a separation medium for the separation of biomolecules, such as polynucleotides, polysaccharides, proteins, and the like, by capillary electrophoresis. Generally, such polymers are characterized by (i) water solubility over the temperature range between about 20° C. to about 50° C., (ii) concentration in a separation medium in the range between about 0.001% to about 10% (weight/volume), (iii) molecular weight in the range of about 5×103 to about 1×106 daltons, and (iv) absence of charged groups in an aqueous medium having pH in the range of about 6 to about 9. In one embodiment, polymers of the invention are selected from the group consisting of polylactams, such as polyvinylpyrrolidone; N,N-disubstituted polyacrylamides; and N-substituted polyacrylamides. In accordance with the method of the invention, a sufficient amount of polymer adsorbs to the capillary surface to establish a zone of high viscosity that shields the analyte from the wall and impedes the movement of an electrical double layer under an electric field.

    Probe composition and method
    19.
    发明申请
    Probe composition and method 失效
    探头组成和方法

    公开(公告)号：US20050112608A1

    公开(公告)日：2005-05-26

    申请号：US10825074

    申请日：2004-04-14

    Applicant: Paul Grossman ,  Steven Fung ,  Steven Menchen ,  Sam Woo ,  Emily Winn-Deen

    Inventor： Paul Grossman ,  Steven Fung ,  Steven Menchen ,  Sam Woo ,  Emily Winn-Deen

    IPC: C12N15/09 ,  B01D57/02 ,  C12Q1/68 ,  G01N27/447 ,  G01N30/88 ,  G01N33/566

    CPC classification number: C12Q1/6869 ,  C12Q1/6816 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6862 ,  Y10T436/143333 ,  C12Q2565/125 ,  C12Q2525/203 ,  C12Q2525/197 ,  C12Q2563/167 ,  C12Q2535/101 ,  C12Q2565/629 ,  C12Q2563/155 ,  C12Q2563/107 ,  C12Q2531/137 ,  C12Q2563/119 ,  C12Q2549/119 ,  C12Q2525/161 ,  C12Q2521/501 ,  C12Q2563/131 ,  C12Q2525/101 ,  C12Q2531/113

    Abstract: Method and composition for detecting one or more selected polynucleotide regions in a target polynucleotide. In one embodiment of the invention, a plurality of different-sequence probe pairs are added to a target polynucleotide, where each probe pair includes two polynucleotide probe elements which are complementary in sequence to adjacent portions of a selected one of the target sequences in the target polynucleotide. In each probe pair, one of the probe elements contains a non-polynucleotide polymer chain which imparts a distinctive mobility to the associated probe pair, when the elements in the pair are ligated. The other element in the pair contains a detectable reporter label. After the probe pairs have been allowed to hybridize with the target polynucleotide, the hybridized polynucleotides are treated under conditions effective to ligate the end subunits of target-bound probe elements when their end subunits are base-paired with adjacent target bases. The ligated probe pairs are then released from the target polynucleotide and separated electrophoretically in a sieving matrix, or chromatographically.

    Abstract translation: 用于检测靶多核苷酸中的一个或多个选择的多核苷酸区域的方法和组合物。 在本发明的一个实施方案中，将多个不同序列探针对加入到靶多核苷酸中，其中每个探针对包含两个多核苷酸探针元件，该多核苷酸探针元件与目标中所选择的一个目标序列的相邻部分互补 多核苷酸。 在每个探针对中，探针元件中的一个包含非多核苷酸聚合物链，当对中的元件被连接时，赋予相关探针对的特异性移动性。 该对中的另一个元素包含可检测的报告物标记。 在探针对已被允许与靶多核苷酸杂交后，杂交的多核苷酸在有效连接靶结合探针元件的末端亚基的条件下处理，当它们的末端亚基与相邻的靶基碱基配对时。 然后将连接的探针对从目标多核苷酸中释放，并在筛分基质中电泳分离，或进行色谱分离。

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