公开(公告)号：US5428696A

公开(公告)日：1995-06-27

申请号：US92162

申请日：1993-07-15

申请人： Jerry E. Cahill ,  David H. Tracy

发明人： Jerry E. Cahill ,  David H. Tracy

IPC分类号： G01N21/25 ,  G02B6/32 ,  G02B6/36 ,  G02B6/42 ,  G02B6/26

CPC分类号： G02B6/3604 ,  G01N21/253 ,  G01N2201/0407 ,  G01N2201/084 ,  G02B6/32 ,  G02B6/4214

摘要： In a spectrophotometer, each of a plurality of source optical fibers is selectively receptive of source radiation and carries the radiation to a corresponding selected liquid sample cell. A corresponding return optical fiber returns transmitted radiation from the sample to a polychromator. For selecting a sample, a switching member holds exposed ends of the optical fibers on a circle coaxial with an axle for rotating to selected positions. Respective optical trains in the instrument direct radiation into and out of the selected pair of fibers. The diameter of a source aperture, the spacing of the aperture from the radiation source, and the source area define a source etendue. The optical fibers have a fiber etendue substantially the same as the source etendue.

摘要翻译： 在分光光度计中，多个源光纤中的每一个选择性地接受源辐射，并将辐射传送到相应的选定液体样品池。 相应的返回光纤将来自样品的透射辐射返回到多色分光器。 为了选择样品，切换构件将光纤的露出端保持在与轴同轴的圆上，以旋转到选定位置。 仪器中的各种光学列车直接辐射进出所选择的一对光纤。 源孔径的直径，孔径与辐射源的距离以及源区域定义了源极化程度。 光纤具有基本上等于光源渗透率的纤维光密度。

公开(公告)号：US5915269A

公开(公告)日：1999-06-22

申请号：US59927

申请日：1998-04-14

申请人： Jerry E. Cahill ,  David H. Tracy

发明人： Jerry E. Cahill ,  David H. Tracy

IPC分类号： G01N30/02 ,  G01N30/60 ,  G01N30/86 ,  G01N30/88 ,  B01D15/08 ,  G06F15/20

CPC分类号： G01N30/8693 ,  G01N30/88 ,  G01N2030/025 ,  G01N2030/8804 ,  G01N30/6095 ,  G01N30/8658

摘要： In a gas chromatograph system having a column with a stationary phase and a carrier gas moving through the column to contact the stationary phase, the system being useful for detecting analytes in a sample, a method and apparatus for predicting the retention times for the analytes under various conditions, the method utilizing the steps of:a) detecting the analytes under a number of sets of given conditions;b) calculating values for various parameters characteristic of the system based on a mathematical model that includes a correction to compensate for the permeability of said column to said carrier gas;c) entering into the model the values for the characteristic parameters and at least one further set of conditions; andd) using the model to predict retention times for conditions other than those of step a;the apparatus composed of a data handling system including a model for predicting retention times at a variety of conditions of operation of the system, the model having a first set of inputs including retention times determined during a number of operating condition sets; and a second input representative of the permeability of the column to the carrier gas, the data handling system possessing a prediction mechanism for putting into the model a set of conditions other than those of a) above and for putting in the set of parameters, and for predicting retention times for those conditions other than those of a).

摘要翻译： 在具有固定相的柱和载气通过柱接触固定相的气相色谱系统中，该系统可用于检测样品中的分析物，用于预测分析物的保留时间的方法和装置 各种条件，所述方法采用以下步骤：a）在多种给定条件下检测分析物; b）基于包括补偿所述柱对所述载气的渗透性的校正的数学模型计算系统的各种参数特性的值; c）向模型中输入特征参数的值和至少一组另外的条件; 以及d）使用该模型预测除了步骤a之外的条件的保留时间; 该装置由包括用于在系统的各种操作条件下预测保留时间的模型的数据处理系统组成，该模型具有包括在多个操作条件集合中确定的保留时间的第一组输入; 以及表示所述列对所述载气的磁导率的第二输入，所述数据处理系统具有预测机构，用于将与上述a）不同的一组条件放入所述模型中，并用于投入所述参数集合;以及 用于预测除a）之外的条件的保留时间。

公开(公告)号：US5928907A

公开(公告)日：1999-07-27

申请号：US752973

申请日：1996-12-02

申请人： Timothy M. Woudenberg ,  Kevin S. Bodner ,  Charles R. Connell ,  Alan M. Ganz ,  Lincoln J. McBride ,  Paul G. Saviano ,  John Shigeura ,  David H. Tracy ,  Eugene F. Young ,  Linda G. Lee

发明人： Timothy M. Woudenberg ,  Kevin S. Bodner ,  Charles R. Connell ,  Alan M. Ganz ,  Lincoln J. McBride ,  Paul G. Saviano ,  John Shigeura ,  David H. Tracy ,  Eugene F. Young ,  Linda G. Lee

IPC分类号： C12N15/09 ,  C12P19/34 ,  C12Q1/68 ,  G01N21/03 ,  G01N21/64 ,  G01N21/75 ,  G01N21/78 ,  G01N33/50 ,  G01N33/58

CPC分类号： G01N21/6428 ,  C12Q1/6818 ,  C12Q1/6851 ,  C12Q1/686 ,  G01N21/0303 ,  G01N2021/6484

摘要： A system is provided for carrying out real time fluorescence-based measurements of nucleic acid amplification products. In a preferred embodiment of the invention, an excitation beam is focused into a reaction mixture through a surface, the reaction mixture containing (i) a first fluorescent indicator capable of generating a first fluorescent signal whose intensity is proportional to the amount of an amplification product in the volume of the reaction mixture illuminated by the excitation beam and (ii) a second fluorescent indicator homogeneously distributed throughout the reaction mixture capable of generating a second fluorescent signal proportional to the volume of reaction mixture illuminated by the excitation beam. Preferably, the excitation beam is focused into the reaction mixture by a lens through a portion of a wall of a closed reaction chamber containing the reaction mixture. The same lens is used to collect the first and second fluorescent signals generated by the first and second fluorescent indicators, respectively, in response to the excitation beam. The ratio of the fluorescent intensities of the first and second fluorescent signals provides a stable quantitative indicator of the amount of amplification product synthesized in the course of the amplification reaction.

摘要翻译： 提供了一种用于进行核酸扩增产物的实时基于荧光的测量的系统。 在本发明的优选实施方案中，激发光束通过表面聚焦到反应混合物中，反应混合物含有（i）第一荧光指示剂，其能够产生第一荧光信号，其强度与扩增产物的量成比例 在由激发束照射的反应混合物的体积中和（ii）均匀分布在整个反应混合物中的第二荧光指示器，其能够产生与由激发束照射的反应混合物的体积成比例的第二荧光信号。 优选地，激发光束通过透镜通过包含反应混合物的封闭反应室的壁的一部分聚焦到反应混合物中。 相同的透镜被用于响应于激发光束分别收集由第一和第二荧光指示器产生的第一和第二荧光信号。 第一和第二荧光信号的荧光强度比提供了在扩增反应过程中合成的扩增产物的量的稳定的定量指标。

公开(公告)号：US5229838A

公开(公告)日：1993-07-20

申请号：US823631

申请日：1992-01-21

申请人： Alan M. Ganz ,  David H. Tracy

发明人： Alan M. Ganz ,  David H. Tracy

IPC分类号： G01J1/00 ,  G01J1/08 ,  G01J3/02 ,  G01M11/00 ,  G01N21/27

CPC分类号： G01J1/08 ,  G01N21/274

摘要： To calibrate a photodetector, a rotating disk with a slot is disposed in a light beam with decreasing speed from a defined maximum rotational speed to a defined minimum speed, while magnitudes and times of signals are read out and stored. Vernier pairs of signals occur in adjacent readout intervals, and non-vernier signals exclude the verniers. Readout times for verniers are used to estimate a preliminary function of rotations versus time. From the function are estimated an occurrence time for each pair and period of disk rotation at the time. Vernier fraction is the ratio of one signal in the pair to the sum of the pair. A time offset is the product of vernier fraction, slot fraction of the disk and the estimated period. Occurrence times corrected with the time offset are utilized to fit a corrected function of disk rotations versus time. Points of time for the non-vernier signals are determined from the corrected function, each point corresponding to disk rotations to a corresponding non-vernier signal. These points of time are employed with corresponding signals for linearly calibrating the photodetector.

公开(公告)号：US07656521B2

公开(公告)日：2010-02-02

申请号：US12119239

申请日：2008-05-12

申请人： Yongdong Wang ,  Bernhard H. Radziuk ,  David H. Tracy

发明人： Yongdong Wang ,  Bernhard H. Radziuk ,  David H. Tracy

IPC分类号： G01J3/00

CPC分类号： G01J3/28 ,  G01J3/42

摘要： In a spectroscopic process a sample for producing a test spectral line or spectrum of at least one component contained in the sample is stimulated and the transmitted and/or emitted electromagnetic rays are used to create the test spectral line or spectrum. In order to improve such a spectroscopic process to such an extent that variations of certain parameters, which alter the shape and/or occurrence of a spectral line, are compensated, a comparison spectral line or spectrum of a known comparison material is produced under substantially the same parameters as the sample. The comparison spectral line or spectrum is compared with an ideal comparison spectral line or spectrum in order to calculate a transfer function, andthe transfer function is applied to the test spectral line or spectrum in order to calculate a corrected test spectral line or spectrum.

摘要翻译： 在分光过程中，刺激用于产生测试谱线或样品中包含的至少一种组分的光谱的样品，并且使用透射和/或发射的电磁波来产生测试光谱线或光谱。 为了改进这种光谱过程，使得补偿了改变光谱线的形状和/或出现的某些参数的变化，已知比较材料的比较光谱线或光谱基本上在 与样品相同的参数。 将比较谱线或频谱与理想的比较谱线或频谱进行比较，以计算传递函数，并将传递函数应用于测试谱线或频谱，以便计算校正的测试谱线或频谱。

公开(公告)号：US07405821B2

公开(公告)日：2008-07-29

申请号：US11552361

申请日：2006-10-24

申请人： Yongdong Wang ,  Bernhard H. Radziuk ,  David H. Tracy

发明人： Yongdong Wang ,  Bernhard H. Radziuk ,  David H. Tracy

IPC分类号： G01J3/00

CPC分类号： G01J3/28 ,  G01J3/42

摘要： In a spectroscopic process a sample for producing a test spectral line or spectrum of at least one component contained in the sample is stimulated and the transmitted and/or emitted electromagnetic rays are used to create the test spectral line or spectrum. In order to improve such a spectroscopic process to such an extent that variations of certain parameters, which alter the shape and/or occurrence of a spectral line, are compensated, a comparison spectral line or spectrum of a known comparison material is produced under substantially the same parameters as the sample. The comparison spectral line or spectrum is compared with an ideal comparison spectral line or spectrum in order to calculate a transfer function, and the transfer function is applied to the test spectral line or spectrum in order to calculate a corrected test spectral line or spectrum.

摘要翻译： 在分光过程中，刺激用于产生测试谱线或样品中包含的至少一种组分的光谱的样品，并且使用透射和/或发射的电磁波来产生测试光谱线或光谱。 为了改进这种光谱过程，使得补偿了改变光谱线的形状和/或出现的某些参数的变化，已知比较材料的比较光谱线或光谱基本上在 与样品相同的参数。 将比较谱线或频谱与理想的比较谱线或频谱进行比较，以计算传递函数，并将传递函数应用于测试谱线或频谱，以便计算校正的测试谱线或频谱。

公开(公告)号：US07145650B2

公开(公告)日：2006-12-05

申请号：US10370910

申请日：2003-02-20

申请人： Yongdong Wang ,  Bernhard H. Radziuk ,  David H. Tracy

发明人： Yongdong Wang ,  Bernhard H. Radziuk ,  David H. Tracy

IPC分类号： G01J3/00 ,  G12B13/00

CPC分类号： G01J3/28 ,  G01J3/42

摘要： In a spectroscopic process a sample for producing a test spectral line or spectrum of at least one component contained in the sample is stimulated and the transmitted and/or emitted electromagnetic rays are used to create the test spectral line or spectrum. In order to improve such a spectroscopic process to such an extent that variations of certain parameters, which alter the shape and/or occurrence of a spectral line, are compensated, a comparison spectral line or spectrum of a known comparison material is produced under substantially the same parameters as the sample. The comparison spectral line or spectrum is compared with an ideal comparison spectral line or spectrum in order to calculate a transfer function, and the transfer function is applied to the test spectral line or spectrum in order to calculate a corrected test spectral line or spectrum.

公开(公告)号：US20080246956A1

公开(公告)日：2008-10-09

申请号：US12119239

申请日：2008-05-12

申请人： Yongdong Wang ,  Bernhard H. Radziuk ,  David H. Tracy

发明人： Yongdong Wang ,  Bernhard H. Radziuk ,  David H. Tracy

IPC分类号： G01N21/00

CPC分类号： G01J3/28 ,  G01J3/42

摘要： In a spectroscopic process a sample for producing a test spectral line or spectrum of at least one component contained in the sample is stimulated and the transmitted and/or emitted electromagnetic rays are used to create the test spectral line or spectrum. In order to improve such a spectroscopic process to such an extent that variations of certain parameters, which alter the shape and/or occurrence of a spectral line, are compensated, a comparison spectral line or spectrum of a known comparison material is produced under substantially the same parameters as the sample. The comparison spectral line or spectrum is compared with an ideal comparison spectral line or spectrum in order to calculate a transfer function, andthe transfer function is applied to the test spectral line or spectrum in order to calculate a corrected test spectral line or spectrum.

摘要翻译： 在分光过程中，刺激用于产生测试谱线或样品中包含的至少一种组分的光谱的样品，并且使用透射和/或发射的电磁波来产生测试光谱线或光谱。 为了改进这种光谱过程，使得补偿了改变光谱线的形状和/或出现的某些参数的变化，已知比较材料的比较光谱线或光谱基本上在 与样品相同的参数。 将比较谱线或频谱与理想的比较谱线或频谱进行比较，以计算传递函数，并将传递函数应用于测试谱线或频谱，以便计算校正的测试谱线或频谱。

公开(公告)号：US09228898B2

公开(公告)日：2016-01-05

申请号：US14008964

申请日：2012-03-27

申请人： Sepehr Kiani ,  Peter Lewis Stokes ,  David H. Tracy

发明人： Sepehr Kiani ,  Peter Lewis Stokes ,  David H. Tracy

IPC分类号： G01J3/28 ,  G01J3/42 ,  G01J3/02 ,  G01J3/06 ,  G01N21/64 ,  G01N15/14 ,  G01J3/44 ,  G01J3/08 ,  G01J3/10 ,  G01J3/14 ,  G01J3/18

CPC分类号： G01J3/42 ,  G01J3/0208 ,  G01J3/021 ,  G01J3/0216 ,  G01J3/0248 ,  G01J3/0291 ,  G01J3/06 ,  G01J3/08 ,  G01J3/10 ,  G01J3/14 ,  G01J3/18 ,  G01J3/2803 ,  G01J3/44 ,  G01J3/4406 ,  G01N15/1434 ,  G01N15/1459 ,  G01N15/1484 ,  G01N21/6456

摘要： The present invention generally pertains to a system, method and kit for the detection and measurement of spectroscopic properties of light from a sample, or the scalable detection and measurement of spectroscopic properties of light from each sample present among multiple samples, simultaneously, wherein the system comprises: an optical train comprising a dispersing element; and an image sensor. The light detected and measured may comprise light scattered from a sample, emitted as chemiluminescence by a chemical process within a sample, selectively absorbed by a sample, or emitted as fluorescence from a sample following excitation.

摘要翻译： 本发明一般涉及一种用于检测和测量来自样品的光的光谱特性的系统，方法和试剂盒，或同时进行多个样品中存在的每个样品的光的光谱特性的可伸缩检测和测量，其中系统 包括：包括分散元件的光学系列; 和图像传感器。 检测和测量的光可以包括从样品散射的光，通过样品内的化学过程作为化学发光发射，被样品选择性吸收，或者在激发后作为荧光从样品中发射。

公开(公告)号：US08373854B2

公开(公告)日：2013-02-12

申请号：US12813389

申请日：2010-06-10

申请人： J. Michael Phillips ,  Kevin S. Bodner ,  Aldrich N. K. Lau ,  Steven J. Boege ,  Mark F. Oldham ,  Donald R. Sandell ,  David H. Tracy

发明人： J. Michael Phillips ,  Kevin S. Bodner ,  Aldrich N. K. Lau ,  Steven J. Boege ,  Mark F. Oldham ,  Donald R. Sandell ,  David H. Tracy

IPC分类号： G01N1/10

CPC分类号： G01N21/278 ,  B82Y15/00 ,  G01J1/10 ,  G01N21/01 ,  G01N21/4785 ,  G01N21/6452 ,  G01N21/76 ,  G01N2201/06113 ,  G01N2201/0612 ,  G01N2201/062 ,  G01N2201/0691 ,  G01N2201/0697 ,  Y10S977/943 ,  Y10S977/949

摘要： The present teachings provide for systems, and components thereof, for detecting and/or analyzing light. These systems can include, among others, optical reference standards utilizing luminophores, such as nanocrystals, for calibrating, validating, and/or monitoring light-detection systems, before, during, and/or after sample analysis.

摘要翻译： 本教导提供了用于检测和/或分析光的系统及其组件。 这些系统尤其可以包括在样品分析之前，期间和/或之后利用发光体（例如纳米晶体）用于校准，验证和/或监测光检测系统的光学参考标准。