公开(公告)号：US20120295339A1

公开(公告)日：2012-11-22

申请号：US13473495

申请日：2012-05-16

申请人： Jiong Wu ,  Mahesh Junnarkar ,  Giacomo Vacca

发明人： Jiong Wu ,  Mahesh Junnarkar ,  Giacomo Vacca

IPC分类号： C12M1/42 ,  C12M1/36

CPC分类号： G01N15/1459 ,  G01N15/1434 ,  G01N21/05 ,  G01N21/53 ,  G01N21/6428 ,  G01N21/645 ,  G01N2021/0346 ,  G01N2021/6482 ,  G01N2021/6493

摘要： Systems and methods for the detection, analysis, and collection of rare cellular events, wherein rare cellular events are defined by events comprising less than 5% of a total number of cells in a sample. The systems and methods generally include: (1) a flow cell dimensioned so as to permit a flow of a sample through the flow cell at a flow rate greater than 300,000 cells per second; (2) a laser positioned to emit a laser beam directed to the flow cell; (3) one or more deflector components disposed between the laser and the flow cell, wherein the deflector component is configured to affect a position of the laser beam relative to the sample flow; (4) one or more fluorescence emission detectors; and (5) one or more processor configured to detect rare cellular events based on fluorescence emission from cell-binding surface markers introduced into the sample prior to the sample being flowed through the flow cell.

摘要翻译： 用于检测，分析和收集罕见细胞事件的系统和方法，其中罕见的细胞事件由包含小于样品中总细胞数量的5％的事件定义。 系统和方法通常包括：（1）流动池，其尺寸设计成允许样品以大于每秒300,000个细胞的流速流过流动池; （2）定位成发射指向流动池的激光束的激光器; （3）设置在所述激光器和所述流动池之间的一个或多个偏转器部件，其中所述偏转器部件被配置为影响所述激光束相对于所述样品流的位置; （4）一个或多个荧光发射检测器; 以及（5）一个或多个处理器，被配置为基于在样品流过所述流动池之前引入到样品中的来自细胞结合表面标记的荧光发射来检测罕见的细胞事件。

公开(公告)号：US08253938B2

公开(公告)日：2012-08-28

申请号：US13116594

申请日：2011-05-26

申请人： Giacomo Vacca ,  Norman R. Goldblatt ,  Michael W. Yee

发明人： Giacomo Vacca ,  Norman R. Goldblatt ,  Michael W. Yee

IPC分类号： G01N21/00

CPC分类号： G01N15/0205 ,  G01N15/0227 ,  G01N15/1459 ,  G01N15/1463 ,  G01N2015/025 ,  G01N2015/1452 ,  G01N2015/1486 ,  G01N2015/1493

摘要： A method for increasing the throughput, or the precision, or both the precision and the throughput, of a flow cytometer, or of a hematology analyzer employing a flow cytometer, by utilizing the technique of laser rastering. Laser rastering involves sweeping a laser beam across a flowing sample stream in a hematology analyzer. An apparatus suitable for carrying out the method of this invention comprises an optical module comprising a source of light, a scanning device, a lens or system of lenses, a flow cell, detectors, and filters; and an electronic module comprising preamplifiers, analog signal conditioning elements, analog-to-digital converters, field-programmable gate arrays, digital signal processing elements, and data storage elements.

摘要翻译： 通过利用激光扫描技术提高流式细胞仪或使用流式细胞仪的血液分析仪的生产量，精度或精度以及吞吐量的方法。 激光扫描涉及通过血液分析仪中的流动样品流扫描激光束。 适用于实施本发明方法的装置包括一个包括光源的光学模块，一个扫描装置，一个透镜或透镜系统，一个流通池，检测器和过滤器; 以及包括前置放大器，模拟信号调节元件，模拟 - 数字转换器，现场可编程门阵列，数字信号处理元件和数据存储元件的电子模块。

公开(公告)号：US20110228271A1

公开(公告)日：2011-09-22

申请号：US13116594

申请日：2011-05-26

申请人： Giacomo Vacca ,  Norman R. Goldblatt ,  Michael W. Yee

发明人： Giacomo Vacca ,  Norman R. Goldblatt ,  Michael W. Yee

IPC分类号： G01N21/00

CPC分类号： G01N15/0205 ,  G01N15/0227 ,  G01N15/1459 ,  G01N15/1463 ,  G01N2015/025 ,  G01N2015/1452 ,  G01N2015/1486 ,  G01N2015/1493

摘要： A method for increasing the throughput, or the precision, or both the precision and the throughput, of a flow cytometer, or of a hematology analyzer employing a flow cytometer, by utilizing the technique of laser rastering. Laser rastering involves sweeping a laser beam across a flowing sample stream in a hematology analyzer. An apparatus suitable for carrying out the method of this invention comprises an optical module comprising a source of light, a scanning device, a lens or system of lenses, a flow cell, detectors, and filters; and an electronic module comprising preamplifiers, analog signal conditioning elements, analog-to-digital converters, field-programmable gate arrays, digital signal processing elements, and data storage elements.

摘要翻译： 通过利用激光扫描技术提高流式细胞仪或使用流式细胞仪的血液分析仪的生产量，精度或精度以及吞吐量的方法。 激光扫描涉及通过血液分析仪中的流动样品流扫描激光束。 适用于实施本发明方法的装置包括一个包括光源的光学模块，一个扫描装置，一个透镜或透镜系统，一个流通池，检测器和过滤器; 以及包括前置放大器，模拟信号调节元件，模拟 - 数字转换器，现场可编程门阵列，数字信号处理元件和数据存储元件的电子模块。

公开(公告)号：US20100273168A1

公开(公告)日：2010-10-28

申请号：US12767611

申请日：2010-04-26

申请人： Martin Krockenberger ,  Jiong Wu ,  Bodo Roemer ,  Giacomo Vacca

发明人： Martin Krockenberger ,  Jiong Wu ,  Bodo Roemer ,  Giacomo Vacca

IPC分类号： C12Q1/68 ,  C12M1/34 ,  C12Q1/06

CPC分类号： G01N15/1404 ,  G01N15/00 ,  G01N15/1429 ,  G01N15/1459 ,  G01N21/53 ,  G01N33/49 ,  G01N33/4915 ,  G01N33/492 ,  G01N2015/0073 ,  G01N2015/0076 ,  G01N2015/008 ,  G01N2015/0084 ,  G01N2015/1006 ,  G01N2015/1486 ,  G01N2800/22 ,  Y10T436/101666

摘要： A method for identifying, analyzing, and quantifying the cellular components of whole blood by means of an automated hematology analyzer and the detection of the light scattered, absorbed, and fluorescently emitted by each cell. More particularly, the aforementioned method involves identifying, analyzing, and quantifying the cellular components of whole blood by means of a light source having a wavelength ranging from about 400 nm to about 450 nm and multiple in-flow optical measurements and staining without the need for lysing red blood cells.

摘要翻译： 一种通过自动血液分析仪识别，分析和定量全血细胞成分的方法，以及每个细胞散射，吸收和荧光发射的光的检测。 更具体地，上述方法包括通过具有波长范围为约400nm至约450nm的光源和多次流入光学测量和染色来鉴定，分析和定量全血的细胞组分，并且不需要 裂解红细胞。

公开(公告)号：US20050168826A1

公开(公告)日：2005-08-04

申请号：US10910756

申请日：2004-08-03

申请人： Serguei Koulikov ,  Giacomo Vacca ,  Alexander Kachanov ,  Bruce Richman ,  Boris Kharlamov ,  Guido Knippels ,  Christopher Rella ,  Hoa Pham

发明人： Serguei Koulikov ,  Giacomo Vacca ,  Alexander Kachanov ,  Bruce Richman ,  Boris Kharlamov ,  Guido Knippels ,  Christopher Rella ,  Hoa Pham

IPC分类号： G02B6/32 ,  G02B6/42 ,  H01S5/022 ,  H01S5/065 ,  H01S5/14 ,  G02B5/32 ,  G02B27/30

CPC分类号： G02B6/4206 ,  G02B6/32 ,  G02B6/4208 ,  G02B6/4226 ,  H01S5/005 ,  H01S5/0064 ,  H01S5/02248 ,  H01S5/02284 ,  H01S5/141 ,  H01S5/4006

摘要： A method and apparatus for adjusting the path of an optical beam and in particular, a method and apparatus for improving the coupling efficiency (power input) of free-space radiation into an optical waveguide using, as part of an optical train, a weak lens positioned along the path of the optical beam (the Z axis) and adapted to adjust the path of the beam. The weak lens is translatable along the Z axis and also along at least one axis perpendicular to the Z axis (the X or Y axes). In a preferred embodiment, the weak lens possesses all three positional degrees of freedom (i.e., it is adjustable along all of the X, Y, and Z axes). In certain preferred embodiments, the weak lens is also capable of one or even two orientational degrees of freedom (i.e., pitch and/or yaw).

公开(公告)号：US20050168825A1

公开(公告)日：2005-08-04

申请号：US10770141

申请日：2004-02-02

申请人： Serguei Koulikov ,  Giacomo Vacca ,  Alexander Kachanov ,  Bruce Richman ,  Boris Kharlamov ,  Guido Knippels ,  Christopher Rella ,  Hoa Pham

发明人： Serguei Koulikov ,  Giacomo Vacca ,  Alexander Kachanov ,  Bruce Richman ,  Boris Kharlamov ,  Guido Knippels ,  Christopher Rella ,  Hoa Pham

IPC分类号： G02B6/32 ,  G02B6/42 ,  G02B27/30

CPC分类号： G02B6/4206 ,  G02B6/32

摘要： A method and apparatus for adjusting the path of an optical beam and in particular, a method and apparatus for improving the coupling efficiency (power input) of free-space radiation into an optical waveguide using, as part of an optical train, a weak lens positioned along the path of the optical beam (the Z axis) and adapted to adjust the path of the beam. The weak lens is translatable along the Z axis and also along at least one axis perpendicular to the Z axis (the X or Y axes). In a preferred embodiment, the weak lens possesses all three positional degrees of freedom (i.e., it is adjustable along all of the X, Y, and Z axes). In certain preferred embodiments, the weak lens is also capable of one or even two orientational degrees of freedom (i.e., pitch and/or yaw).

摘要翻译： 一种用于调整光束路径的方法和装置，特别地，一种用于提高自由空间辐射到光波导中的耦合效率（功率输入）的方法和装置，其使用作为光学系列的一部分的弱透镜 沿着光束的路径（Z轴）定位并且适于调整光束的路径。 弱透镜可沿Z轴平移，也可沿垂直于Z轴（X或Y轴）的至少一个轴平移。 在优选实施例中，弱透镜具有全部三个位置自由度（即，沿着X，Y和Z轴全部可调节）。 在某些优选实施例中，弱透镜还能够具有一个或甚至两个取向自由度（即俯仰和/或偏转）。

公开(公告)号：US09097704B2

公开(公告)日：2015-08-04

申请号：US13093502

申请日：2011-04-25

申请人： Jiong Wu ,  Giacomo Vacca

发明人： Jiong Wu ,  Giacomo Vacca

IPC分类号： G01N15/14 ,  G01N15/10 ,  G01N33/50 ,  G01N33/58 ,  G01N15/00

CPC分类号： G01N15/1434 ,  G01N15/147 ,  G01N21/6428 ,  G01N33/5047 ,  G01N33/5094 ,  G01N33/582 ,  G01N35/1002 ,  G01N2015/0065 ,  G01N2015/0069 ,  G01N2015/1006 ,  G01N2015/1486 ,  G01N2015/149 ,  G01N2021/6439

摘要： A method whereby one or more fluorescent dyes are used to bind and stain nucleic acids in certain blood cells, such as, for example, white blood cells, nucleated red blood cells, and reticulocytes, and to induce fluorescent emissions upon excitation of photons from a given source of light, such as, for example, a laser, at an appropriate wavelength. More particularly, this invention provides a method whereby a fluorescent trigger is used in a data collection step for collecting events that emit strong fluorescence, in order to separate white blood cells and nucleated red blood cells from red blood cells and platelets without the need for using a lysing agent.

摘要翻译： 一种或多种荧光染料用于结合和染色某些血细胞（例如白细胞，有核红细胞和网织红细胞）中的核酸的方法，以及在从 给定光源，例如激光，以适当的波长。 更具体地，本发明提供了一种方法，其中在用于收集发射强荧光的事件的数据收集步骤中使用荧光触发器，以将白细胞和有核红细胞与红细胞和血小板分离而不需要使用 裂解剂。

公开(公告)号：US08715572B2

公开(公告)日：2014-05-06

申请号：US13473495

申请日：2012-05-16

申请人： Jiong Wu ,  Mahesh Junnarkar ,  Giacomo Vacca

发明人： Jiong Wu ,  Mahesh Junnarkar ,  Giacomo Vacca

IPC分类号： G01N21/64 ,  G01N15/06 ,  G06K9/00 ,  G01N15/02 ,  G01N21/49 ,  G01N21/85 ,  G01J3/30 ,  G01N21/00

CPC分类号： G01N15/1459 ,  G01N15/1434 ,  G01N21/05 ,  G01N21/53 ,  G01N21/6428 ,  G01N21/645 ,  G01N2021/0346 ,  G01N2021/6482 ,  G01N2021/6493

摘要： Systems and methods for the detection, analysis, and collection of rare cellular events, wherein rare cellular events are defined by events comprising less than 5% of a total number of cells in a sample. The systems and methods generally include: (1) a flow cell dimensioned so as to permit a flow of a sample through the flow cell at a flow rate greater than 300,000 cells per second; (2) a laser positioned to emit a laser beam directed to the flow cell; (3) one or more deflector components disposed between the laser and the flow cell, wherein the deflector component is configured to affect a position of the laser beam relative to the sample flow; (4) one or more fluorescence emission detectors; and (5) one or more processor configured to detect rare cellular events based on fluorescence emission from cell-binding surface markers introduced into the sample prior to the sample being flowed through the flow cell.

摘要翻译： 用于检测，分析和收集罕见细胞事件的系统和方法，其中罕见的细胞事件由包含小于样品中总细胞数量的5％的事件定义。 系统和方法通常包括：（1）流动池，其尺寸设计成允许样品以大于每秒300,000个细胞的流速流过流动池; （2）定位成发射指向流动池的激光束的激光器; （3）设置在所述激光器和所述流动池之间的一个或多个偏转器部件，其中所述偏转器部件被配置为影响所述激光束相对于所述样品流的位置; （4）一个或多个荧光发射检测器; 以及（5）一个或多个处理器，被配置为基于在样品流过所述流动池之前引入到样品中的来自细胞结合表面标记物的荧光发射来检测罕见的细胞事件。

公开(公告)号：US20120282598A1

公开(公告)日：2012-11-08

申请号：US13456729

申请日：2012-04-26

申请人： Jiong Wu ,  Giacomo Vacca

发明人： Jiong Wu ,  Giacomo Vacca

IPC分类号： C12M1/34 ,  G01N21/64

CPC分类号： G01N15/1434 ,  G01N15/1459 ,  G01N21/6428 ,  G01N33/49 ,  G01N2015/008 ,  G01N2015/1006 ,  G01N2015/1477 ,  G01N2015/1488 ,  G01N2021/6439

摘要： Systems and methods for analyzing blood samples, and more specifically for performing a white blood cell (WBC) differential analysis. The systems and methods screen WBCs by means of fluorescence staining and a fluorescence triggering strategy. As such, interference from unlysed red blood cells (RBCs) and fragments of lysed RBCs is substantially eliminated. The systems and methods also enable development of relatively milder WBC reagent(s), suitable for assays of samples containing fragile WBCs. In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye, which corresponds in emission spectrum to an excitation source of a hematology instrument; (b) using a fluorescence trigger to screen the blood sample for WB Cs; and (c) using measurements of (1) axial light loss, (2) intermediate angle scatter, (3) 90° polarized side scatter, (4) 90° depolarized side scatter, and (5) fluorescence emission to perform a differentiation analysis.

摘要翻译： 用于分析血液样品的系统和方法，更具体来说用于进行白细胞（WBC）差异分析。 系统和方法通过荧光染色和荧光触发策略筛选WBC。 因此，基本上消除了来自未分解的红细胞（RBC）和裂解的RBC的片段的干扰。 这些系统和方法还可以开发出相对温和的WBC试剂，适用于含有脆弱WBC的样品的测定。 在一个实施方案中，系统和方法包括：（a）用发射光谱对应于血液学仪器的激发源的专用细胞膜可渗透荧光染料染色血液样品; （b）使用荧光触发器筛选WB Cs的血样; 和（c）使用（1）轴向光损失，（2）中角散射，（3）90°偏振侧散射，（4）90°去极化侧散射的测量，和（5）荧光发射来进行微分分析 。

公开(公告)号：US20110275064A1

公开(公告)日：2011-11-10

申请号：US13093502

申请日：2011-04-25

申请人： Jiong Wu ,  Giacomo Vacca

发明人： Jiong Wu ,  Giacomo Vacca

IPC分类号： C12Q1/68

CPC分类号： G01N15/1434 ,  G01N15/147 ,  G01N21/6428 ,  G01N33/5047 ,  G01N33/5094 ,  G01N33/582 ,  G01N35/1002 ,  G01N2015/0065 ,  G01N2015/0069 ,  G01N2015/1006 ,  G01N2015/1486 ,  G01N2015/149 ,  G01N2021/6439

摘要： A method whereby one or more fluorescent dyes are used to bind and stain nucleic acids in certain blood cells, such as, for example, white blood cells, nucleated red blood cells, and reticulocytes, and to induce fluorescent emissions upon excitation of photons from a given source of light, such as, for example, a laser, at an appropriate wavelength. More particularly, this invention provides a method whereby a fluorescent trigger is used in a data collection step for collecting events that emit strong fluorescence, in order to separate white blood cells and nucleated red blood cells from red blood cells and platelets without the need for using a lysing agent.

摘要翻译： 一种或多种荧光染料用于结合和染色某些血细胞（例如白细胞，有核红细胞和网织红细胞）中的核酸的方法，以及在从 给定光源，例如激光，以适当的波长。 更具体地，本发明提供了一种方法，其中在用于收集发射强荧光的事件的数据收集步骤中使用荧光触发器，以将白细胞和有核红细胞与红细胞和血小板分离而不需要使用 裂解剂。