公开(公告)号：US08528744B2

公开(公告)日：2013-09-10

申请号：US12680531

申请日：2008-09-26

Applicant: Bernd Krause ,  Markus Hornung ,  Silvia Koch ,  Doris Deppisch

Inventor： Bernd Krause ,  Markus Hornung ,  Reinhold Deppisch ,  Silvia Koch

IPC: B01D71/00 ,  B01D69/00 ,  B01D69/08 ,  B01D67/00

CPC classification number: B01D69/02 ,  A61M1/16 ,  A61M1/3496 ,  B01D67/0088

Abstract: The invention relates to hydrophilic membranes which are supplemented or treated with a non-ionic surfactant and processes for preparing such membranes. The membranes are particularly suitable for plasma separation or for haemodialysis and haemodiafiltration, but can also advantageously be used in other applications. Accordingly, the invention is further directed to the use of such membranes for plasma separation, plasma filtration, micro filtration, plasma therapy, haemodialysis and haemodiafiltration or cell filtration applications, respectively. The treated hydrophilic membranes show excellent biocompatibility, such as reduced platelet drop and decreased TAT levels.

Abstract translation: 本发明涉及用非离子表面活性剂补充或处理的亲水膜及其制备方法。 该膜特别适用于血浆分离或用于血液透析和血液透析过滤，但也可有利地用于其它应用。 因此，本发明进一步涉及这种膜用于血浆分离，血浆过滤，微过滤，血浆治疗，血液透析和血液透析或细胞过滤应用。 经处理的亲水膜显示出优异的生物相容性，例如减少血小板降低和降低的TAT水平。

公开(公告)号：US08435751B2

公开(公告)日：2013-05-07

申请号：US13120397

申请日：2009-09-23

Applicant: Carina Zweigart ,  Bernd Krause ,  Markus Neubauer ,  Doris Deppisch

Inventor： Carina Zweigart ,  Bernd Krause ,  Markus Neubauer ,  Reinhold Deppisch

IPC: G01N33/53

CPC classification number: C12N5/0068 ,  A61L27/26 ,  A61L27/38 ,  B01D67/009 ,  B01D67/0095 ,  B01D69/02 ,  B01D69/06 ,  B01D69/08 ,  B01D71/44 ,  B01D71/54 ,  B01D71/56 ,  B01D71/68 ,  B01D2323/34 ,  B01D2325/36 ,  C12N2533/30 ,  C08L81/06

Abstract: A membrane which can be used for cultivating cells, in particular adherent cells. The membrane permits the adhesion and proliferation of the cells based on its specific composition comprising polyurethane. The resulting surface characteristics further permit the membrane to be used without any pre-treatment with surface modifying substances. A method for preparing a membrane which can be used for cultivating cells, in particular adherent cells. Methods of using the membrane for cultivating cells, in particular adherent cells.

Abstract translation: 可用于培养细胞，特别是贴壁细胞的膜。 该膜允许基于其包含聚氨酯的特定组成的细胞的粘附和增殖。 所得到的表面特性进一步允许使用膜，而不用表面改性物质进行任何预处理。 一种制备可用于培养细胞，特别是贴壁细胞的膜的方法。 使用膜用于培养细胞，特别是贴壁细胞的方法。

公开(公告)号：US20120208256A1

公开(公告)日：2012-08-16

申请号：US13503204

申请日：2010-10-20

Applicant: Ralf Flieg ,  Manuela Klotz ,  Markus Storr ,  Martin Rempfer ,  Wolfgang Freudemann ,  Cornelia Winz ,  Bernd Krause ,  Markus Hornung

Inventor： Ralf Flieg ,  Manuela Klotz ,  Markus Storr ,  Martin Rempfer ,  Wolfgang Freudemann ,  Cornelia Winz ,  Bernd Krause ,  Markus Hornung

IPC: C12N11/06 ,  C12N11/02

CPC classification number: C12N9/18 ,  C09D133/14 ,  C12N9/80 ,  C12N11/06 ,  C12N11/08

Abstract: An immobilized enzyme comprises a solid support, an enzyme linked to the solid support and a spacer for coupling the enzyme to the solid support. A method for the production of immobilized enzymes comprises: providing a solid support having amino-functional groups coupled to the support surface; covalently coupling the amino-functional groups with a thermally labile radical initiator; and, contacting the support surface with a solution of polymerizable monomers. The polymerizable monomers comprise functional groups which do not take part in radical polymerization, under conditions where thermally initiated graft copolymerization of the monomers takes place, to form polymer chains on the surface of the support. If the polymer chains do not already comprise primary amino-functional groups, the method further comprises transforming the functional groups in the polymer chains into groups comprising primary amino-functional groups. The method further comprises coupling the enzyme to amino-functional groups of the polymer chains.

Abstract translation: 固定化酶包括固体支持物，与固体支持物连接的酶和用于将酶偶联到固体支持物的间隔物。 制备固定化酶的方法包括：提供具有与支持表面偶联的氨基官能团的固体支持物; 将氨基官能团与热不稳定的自由基引发剂共价偶联; 并且使支撑体表面与可聚合单体的溶液接触。 可聚合单体包括在发生单体的热引发接枝共聚的条件下不参与自由基聚合的官能团，以在载体的表面上形成聚合物链。 如果聚合物链不包括伯氨基官能团，则该方法还包括将聚合物链中的官能团转化为包含伯氨基官能团的基团。 该方法还包括将酶偶联到聚合物链的氨基官能团。

公开(公告)号：US20120077096A1

公开(公告)日：2012-03-29

申请号：US12893040

申请日：2010-09-29

Applicant: Bernd Krause

Inventor： Bernd Krause

IPC: H01M8/04 ,  H01M8/06

CPC classification number: H01M8/04231 ,  B01D2257/104 ,  B01D2258/0208 ,  H01M8/04156 ,  H01M8/04753 ,  H01M8/04955 ,  H01M8/0662 ,  H01M2008/1095

Abstract: A fuel cell system comprises an oxygen removing device having an inlet fluidly connected to at least one of the reactant gas source and an outlet of the cathode gas flow field, and an outlet fluidly connected to each of an anode control valve and a cathode control valve. Various fuel cell operation processes are also disclosed.

Abstract translation: 燃料电池系统包括除氧装置，其具有流体连接到反应气体源和阴极气体流场的出口中的至少一个的入口，以及流体连接到阳极控制阀和阴极控制阀中的每一个的出口 。 还公开了各种燃料电池操作过程。

公开(公告)号：US20120074064A1

公开(公告)日：2012-03-29

申请号：US13320699

申请日：2010-05-19

Applicant: Bernd Krause ,  Carina Zweigart ,  Heinrich Behr

Inventor： Bernd Krause ,  Carina Zweigart ,  Heinrich Behr

IPC: B01D71/68 ,  B29C47/08 ,  B01D71/42 ,  B01D61/00 ,  B01D69/08 ,  B01D71/56

CPC classification number: B01D69/088 ,  A61M1/16 ,  A61M1/34 ,  A61M1/3403 ,  A61M1/341 ,  A61M1/3413 ,  A61M1/3496 ,  A61M2205/15 ,  B01D71/56 ,  B01D71/68

Abstract: Permselective asymmetric membranes suitable e.g. for hemodialysis, hemodiafiltration and hemofiltration of blood, and having improved performance, including improved sieving characteristics, providing enhanced removal of middle molecular weight substances, e.g. inflammatory mediators having a molecular weight between 20 and 40 kDa. The improved sieving characteristics are due to a narrow pore size distribution of the membranes created in the production process. Processes for the preparation of these membranes, devices comprising these membranes, and the use of these membranes in hemodialysis, hemodiafiltration and hemofiltration of blood, as well as in bioprocessing, plasma fractionation and the preparation of protein solutions.

Abstract translation: 选择性不对称膜适合于例如 用于血液透析，血液透析过滤和血液的血液滤过，并且具有改善的性能，包括改善的筛分特性，提供中等分子量物质的增强的去除。 具有20至40kDa分子量的炎性介质。 改进的筛分特性是由于在生产过程中产生的膜的孔径分布窄。 用于制备这些膜的方法，包括这些膜的装置以及这些膜在血液透析，血液透析滤过和血液血液滤过以及生物处理，血浆分离和蛋白质溶液的制备中的用途。

公开(公告)号：US20120074063A1

公开(公告)日：2012-03-29

申请号：US13320679

申请日：2010-05-19

Applicant: Bernd Krause ,  Carina Zweigart

Inventor： Bernd Krause ,  Carina Zweigart

IPC: B01D71/68 ,  B29C47/12 ,  B01D67/00 ,  B01D61/24 ,  B01D69/08

CPC classification number: B01D69/08 ,  B01D67/0088 ,  B01D67/0093 ,  B01D71/40 ,  B01D71/68 ,  B01D71/76

Abstract: Permselective asymmetric membranes suitable e.g. for hemodialysis, hemodiafiltration and hemofiltration of blood, and having improved sieving characteristics providing enhanced removal of middle molecular weight substances, e.g. inflammatory mediators having a molecular weight between 20 and 40 kDa. The membranes comprise polyethersulfone and polyvinylpyrrolidone and are coated with hyaluronic acid or copolymers of 2-methacryloyloxyethyl phosphoryl choline and other vinyl polymerizable monomers. Processes for the preparation of these membranes, devices comprising these membranes, and the use of these membranes in hemodialysis, hemodiafiltration and hemofiltration of blood, as well as in bioprocessing, plasma fractionation and the preparation of protein solutions.

Abstract translation: 选择性不对称膜适合于例如 用于血液透析，血液透析过滤和血液的血液滤过，并且具有改善的筛分特性，提供中等分子量物质的增强除去。 具有20至40kDa分子量的炎性介质。 膜包含聚醚砜和聚乙烯吡咯烷酮，并用透明质酸或2-甲基丙烯酰氧基乙基磷酰胆碱和其它乙烯基可聚合单体的共聚物涂覆。 用于制备这些膜的方法，包括这些膜的装置以及这些膜在血液透析，血液透析滤过和血液血液滤过以及生物处理，血浆分离和蛋白质溶液的制备中的用途。

公开(公告)号：US20120067815A1

公开(公告)日：2012-03-22

申请号：US13120298

申请日：2009-09-23

Applicant: David Luttropp ,  Bernd Krause ,  Markus Neubauer ,  Reinhold Deppisch ,  Doris Deppisch ,  Andrea Schnell ,  Helmut Geiger ,  Juergen Bereiter-Hahn ,  Patrick Baer

Inventor： David Luttropp ,  Bernd Krause ,  Markus Neubauer ,  Reinhold Deppisch ,  Doris Deppisch ,  Andrea Schnell ,  Helmut Geiger ,  Juergen Bereiter-Hahn ,  Patrick Baer

IPC: A61M1/16 ,  B01D63/04 ,  B01D61/28 ,  B01D61/24 ,  A61M1/14

CPC classification number: A61M1/3472 ,  A61M1/3489 ,  A61M1/3687 ,  A61M1/3689 ,  B01D69/08 ,  B01D69/144 ,  C12N5/0686 ,  C12N2500/14 ,  C12N2500/84 ,  C12N2501/11 ,  C12N2533/30

Abstract: A bioartificial kidney equivalent and a process for producing the bioartificial kidney equivalent. The hybrid bioartificial kidney comprises human proximal and distal renal tubule cells grown on particular synthetic membranes.

Abstract translation: 生物人造肾等效物和生物人造肾当量的生产方法。 混合生物人造肾包括在特定合成膜上生长的人近端和远端的肾小管细胞。

公开(公告)号：US20110178743A1

公开(公告)日：2011-07-21

申请号：US12691511

申请日：2010-01-21

Applicant: Bernd Krause

Inventor： Bernd Krause

IPC: G01R31/36 ,  G01R19/00 ,  G06F19/00

CPC classification number: H01M8/04552 ,  G01R31/362 ,  H01M8/04671

Abstract: A method for determining a low performing cell in a fuel cell stack. The method measures the voltage of each cell in the fuel cell stack and calculates an average cell voltage of all of the cell voltages from the fuel cell stack at a plurality of stack current densities. The method also identifies a minimum cell voltage from all of the cell voltages from the fuel cell stack at the plurality of stack current densities that the average cell voltages are calculated and determines a relative delta voltage relationship between the average cell voltage and the minimum cell voltage at each of the plurality of stack current densities. The relative delta voltage relationships are used to determine whether the minimum cell voltage indicates a persistent stack problem.

Abstract translation: 一种用于确定燃料电池堆中的低效电池的方法。 该方法测量燃料电池堆中每个电池的电压，并以多个堆电流密度计算来自燃料电池堆的所有电池电压的平均电池电压。 该方法还根据计算平均电池电压的多个堆叠电流密度从燃料电池堆的所有电池电压中识别最小电池电压，并且确定平均电池电压和最小电池电压之间的相对增量电压关系 在多个堆叠电流密度中的每一个处。 使用相对的delta电压关系来确定最小电池电压是否表示持续堆栈问题。

公开(公告)号：US07955752B2

公开(公告)日：2011-06-07

申请号：US11142762

申请日：2005-06-01

Applicant: Bernd Krause ,  Joerg Roth

Inventor： Bernd Krause ,  Joerg Roth

IPC: H01M8/04

CPC classification number: H01M8/0265 ,  H01M8/0258 ,  H01M8/04082 ,  H01M8/241 ,  H01M8/2457 ,  H01M2008/1095

Abstract: A fuel cell that includes specially configured bipolar plates that separate the reactant gas flow field in an active area of the fuel cell into a primary flow channels and a secondary channels. In one embodiment, the primary flow channels are in use over the entire operating range of the fuel cell and the secondary flow channels are only in use at high cell current outputs. At low power demands, the primary channels operate at a voltage below 0.8 volts and provides a gas current density of more than 0.2 A/cm2. The secondary flow channels have no gas supply and operate at mass transport limited conditions. Because of this design, voltage cycling is significantly reduced or eliminated, thus increasing the life of the fuel cell.

Abstract translation: 一种燃料电池，其包括特殊配置的双极板，其将燃料电池的有效区域中的反应气体流场分离成主流道和次流道。 在一个实施例中，主流动通道在燃料电池的整个操作范围内使用，并且次级流动通道仅在高电池电流输出时使用。 在低功率需求下，主通道工作在低于0.8伏的电压，并提供大于0.2A / cm2的气体电流密度。 二次流动通道没有气体供应并且在大规模运输有限的条件下运行。 由于这种设计，电压循环显着降低或消除，从而增加燃料电池的寿命。

公开(公告)号：US20100092817A1

公开(公告)日：2010-04-15

申请号：US12637501

申请日：2009-12-14

Applicant: Sebastian Lienkamp ,  Bernd Krause

Inventor： Sebastian Lienkamp ,  Bernd Krause

IPC: H01M8/04

CPC classification number: H01M8/04992 ,  H01M8/04552 ,  H01M8/04559 ,  H01M8/04649 ,  H01M2008/1095 ,  H01M2250/20 ,  Y02T90/32

Abstract: A method for revising a reference polarization curve of a fuel cell stack that identifies the relationship between the voltage and the current of the stack over time. When the stack is operating at a low load where kinetic voltage losses of the stack dominate, a first adaptation value is revised as the difference between the actual stack voltage and the stack voltage of the reference polarization curve. When the stack is operating at higher loads where ohmic voltage losses of the stack dominate, a second adaptation value is revised as the difference between the actual stack voltage and the stack voltage of the reference polarization curve.

Abstract translation: 一种用于修改燃料电池堆的参考极化曲线的方法，其识别堆叠随时间的电压和电流之间的关系。 当堆栈以堆叠的动态电压损失为主的低负载运行时，第一适配值被修改为实际堆叠电压与参考极化曲线的堆叠电压之间的差值。 当堆栈工作在堆叠的欧姆电压损耗主导的较高负载时，第二适配值被修改为实际堆叠电压和参考极化曲线的堆叠电压之间的差。