中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

20 records (took 0.031 seconds)

    Cis/trans riboregulators
    13.
    发明申请
    Cis/trans riboregulators 有权
    顺式/反式核糖调节剂

    公开(公告)号：US20070136827A1

    公开(公告)日：2007-06-14

    申请号：US10535128

    申请日：2003-11-14

    Applicant: James Collins ,  Farren Isaacs ,  Charles Cantor ,  Daniel Dwyer

    Inventor： James Collins ,  Farren Isaacs ,  Charles Cantor ,  Daniel Dwyer

    IPC: A01K67/027 ,  C07H21/04 ,  C12N15/09 ,  C12N5/06

    CPC classification number: C12N15/67 ,  C12N15/11 ,  C12N2310/53 ,  C12Q1/6897

    Abstract: The present invention provides nucleic acid molecules, DNA constructs, plasmids, and methods for post-transcriptional regulation of gene expression using RNA molecules to both repress and activate translation of an open reading frame. Repression of gene expression is achieved through the presence of a regulatory nucleic acid element (the cis-repressive RNA or crRNA) within the 5′ untranslated region (5′ UTR) of an mRNA molecule. The nucleic acid element forms a hairpin (stem/loop) structure through complementary base pairing. The hairpin blocks access to the mRNA transcript by the ribosome, thereby preventing translation. In particular, in embodiments of the invention designed to operate in prokaryotic cells, the stem of the hairpin secondary structure sequesters the ribosome binding site (RBS). In embodiments of the invention designed to operate in eukaryotic cells, the stem of the hairpin is positioned upstream of the start codon, anywhere within the 5′ UTR of an mRNA. A small RNA (trans-activating RNA, or taRNA), expressed in trans, interacts with the crRNA and alters the hairpin structure. This alteration allows the ribosome to gain access to the region of the transcript upstream of the start codon, thereby activating transcription from its previously repressed state.

    Abstract translation: 本发明提供核酸分子，DNA构建体，质粒和用于转录后调节基因表达的方法，其使用RNA分子抑制和激活开放阅读框的翻译。 通过在mRNA分子的5'非翻译区（5'UTR）内存在调节性核酸元件（顺式抑制性RNA或crRNA）来实现基因表达的抑制。 核酸元件通过互补碱基配对形成发夹（茎/环）结构。 发夹阻止核糖体进入mRNA转录物，从而阻止翻译。 特别地，在设计用于在原核细胞中操作的本发明的实施方案中，发夹二级结构的茎螯合核糖体结合位点（RBS）。 在设计用于在真核细胞中操作的本发明的实施方案中，发夹的茎位于起始密码子的上游，位于mRNA的5'UTR内的任何位置。 以反式表达的小RNA（反式激活RNA或taRNA）与crRNA相互作用并改变发夹结构。 这种改变允许核糖体进入起始密码子上游的转录物区域，从而从其先前的抑制状态激活转录。

    Methods for prenatal diagnosis of chromosomal abnormalities
    14.
    发明申请
    Methods for prenatal diagnosis of chromosomal abnormalities 有权
    产前诊断染色体异常的方法

    公开(公告)号：US20070059707A1

    公开(公告)日：2007-03-15

    申请号：US10575119

    申请日：2004-10-08

    Applicant: Charles Cantor ,  Chunming Ding

    Inventor： Charles Cantor ,  Chunming Ding

    IPC: C12Q1/68 ,  C12P19/34

    CPC classification number: C12Q1/6883 ,  C12Q1/6806 ,  C12Q1/6827 ,  C12Q1/6876 ,  C12Q2600/154 ,  C12Q2600/156 ,  C12Q2600/166 ,  C12Q2521/331

    Abstract: Chromosomal abnormalities are responsible for a significant number of birth defects, including mental retardation. The present invention is related to methods for non-invasive and rapid, prenatal diagnosis of chromosomal abnormalities based on analysis of a maternal blood sample. The invention exploits the differences in DNA between the mother and fetus, for instance differences in their methylation states, as a means to enrich for fetal DNA in maternal plasma sample. The methods described herein can be used to detect chromosomal DNA deletions and duplications. In a preferred embodiment, the methods are used to diagnose chromosomal aneuploidy and related disorders, such as Down's and Turner's Syndrome.

    Abstract translation: 染色体异常负责大量的出生缺陷，包括精神发育迟滞。 本发明涉及基于母体血液样品分析的非侵入性和快速，产前诊断染色体异常的方法。 本发明利用母体和胎儿之间的DNA差异，例如甲基化状态的差异，作为富集母体血浆样品中胎儿DNA的手段。 本文描述的方法可用于检测染色体DNA缺失和重复。 在优选的实施方案中，所述方法用于诊断染色体非整倍体和相关疾病，例如唐氏和特纳综合征。

    Haplotype analysis
    16.
    发明申请
    Haplotype analysis 有权
    单倍型分析

    公开(公告)号：US20070122805A1

    公开(公告)日：2007-05-31

    申请号：US10542043

    申请日：2004-01-16

    Applicant: Charles Cantor ,  Chunming Ding

    Inventor： Charles Cantor ,  Chunming Ding

    IPC: C12Q1/68 ,  C12P19/34

    CPC classification number: C12Q1/6827 ,  C12Q1/6858 ,  C12Q2600/156 ,  C12Q2527/137 ,  C12Q2537/143 ,  C12Q2565/627

    Abstract: The present invention provides an efficient way for high throughput haplotype analysis. Several polymorphic nucleic add markers, such as SNPs, can be simultaneously and reliably determined through multiplex PCR of single nucleic acid molecules in several parallel single molecule dilutions and the consequent statistical analysis of the results from these parallel single molecule multiplex PCR reactions results in reliable determination of haplotypes present in the subject. The nucleic acid markers can be of any distance to each other on the chromosome. In addition, an approach wherein overlapping DNA markers are analyzed can be used to link smaller haplotypes into larger haplotypes. Consequently, the invention provides a powerful new tool for diagnostic haplotyping and identifying novel haplotypes.

    Abstract translation: 本发明提供了高通量单倍型分析的有效方法。 可以通过多个平行的单分子稀释物中的单个核酸分子的多重PCR同时且可靠地测定几种多态性核酸添加标记，例如SNP，并且随后对这些平行单分子多重PCR反应的结果的统计分析导致可靠的测定 的单倍体存在于受试者。 核酸标记在染色体上可以彼此有任何距离。 此外，分析重叠的DNA标记物的方法可用于将较小的单倍型连接到较大的单元型中。 因此，本发明为诊断单倍型和鉴定新型单体型提供了强大的新工具。

    Nucleic acid supported protein complementation
    18.
    发明申请
    Nucleic acid supported protein complementation 有权
    核酸支持的蛋白质互补

    公开(公告)号：US20060094014A1

    公开(公告)日：2006-05-04

    申请号：US10529122

    申请日：2003-10-09

    Applicant: Charles Cantor ,  Natalia Broude ,  Carlos Witte-Hoffmann

    Inventor： Charles Cantor ,  Natalia Broude ,  Carlos Witte-Hoffmann

    IPC: C12Q1/68 ,  C07H21/00 ,  A61K49/00

    CPC classification number: C12Q1/6818 ,  C12Q1/6813 ,  C12Q2563/131 ,  C12Q2561/107

    Abstract: The present invention is directed to novel methods for in vitro and in vivo detection of target nucleic acid molecules, including DNA and RNA targets, as well as nucleic acid analogues. The present invention is based on protein complementation, in which two individual polypeptides are inactive. When the two inactive polypeptide fragment are brought in close proximity during hybridization to a target nucleic acid, they re-associate into an active, detectable protein.

    Abstract translation: 本发明涉及用于靶核酸分子（包括DNA和RNA靶标）以及核酸类似物的体外和体内检测的新方法。 本发明基于蛋白质互补，其中两个单独的多肽是无活性的。 当两个无活性多肽片段在与靶核酸杂交期间紧密接近时，它们重新连接成活性的可检测蛋白质。

Patent Agency Ranking