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公开(公告)号：US20140227754A1
公开(公告)日：2014-08-14
申请号：US14172790
申请日：2014-02-04
Applicant: Danisco US Inc.
Inventor： Wolfgang Aehle , Richard R. Bott , Martijn Scheffers , Pieter van Solingen , Casper Vroemen
CPC classification number: C12N9/2428 , C12P7/06 , C12P19/02 , C12P19/14 , Y02E50/17
Abstract: The present disclosure relates to variants of a parent glucoamylase having altered properties (e.g., improved thermostability and/or specific activity). In particular, the present disclosure provides compositions comprising the variant glucoamylases, including starch hydrolyzing compositions and cleaning compositions. The disclosure also relates to DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.
Abstract translation: 本公开涉及具有改变的性质（例如改善的热稳定性和/或比活性）的母体葡糖淀粉酶的变体。 特别地，本公开提供了包含变体葡糖淀粉酶的组合物，包括淀粉水解组合物和清洁组合物。 本公开还涉及编码在宿主细胞中产生葡糖淀粉酶变体的变体和方法的DNA构建体。
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公开(公告)号：US20190002937A1
公开(公告)日：2019-01-03
申请号：US16064552
申请日：2016-12-20
Applicant: Danisco US Inc.
Inventor： Bart C. Koops , Paula Johanna Maria Teunissen , Marco Van Brussel-Zwijnen , Martijn Scheffers , Kees-Jan Guijt , Zhengzheng Zou , Zhongmei Tang , Zhen Qian , Jing Ge , Zhenghong Zhang
Abstract: Described are methods and compositions relating to granular starch-converting glucoamylases and α-amylases. The enzymes can be used to perform enzymatic starch hydrolysis of granular starch at or below the gelatinization temperature of insoluble granular starch.
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公开(公告)号：US09944913B2
公开(公告)日：2018-04-17
申请号：US15230036
申请日：2016-08-05
Applicant: Danisco US Inc.
Inventor： Wolfgang Aehle , Piet Van Solingen , Martijn Scheffers , Richard Bott
CPC classification number: C12N9/2428 , C12N15/09 , C12Y302/01003 , Y02E50/17
Abstract: The present invention relates to variants of a parent glucoamylase having altered properties (e.g., improved thermostability and/or specific activity). In particular, the present invention provides compositions comprising the variant glucoamylases, including starch hydrolyzing compositions, animal feed compositions and cleaning compositions. The invention also relates to DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.
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公开(公告)号：US20170114336A1
公开(公告)日：2017-04-27
申请号：US15230036
申请日：2016-08-05
Applicant: Danisco US Inc.
Inventor： Wolfgang Aehle , Piet Van Solingen , Martijn Scheffers , Richard Bott
IPC: C12N9/34
CPC classification number: C12N9/2428 , C12N15/09 , C12Y302/01003 , Y02E50/17
Abstract: The present invention relates to variants of a parent glucoamylase having altered properties (e.g., improved thermostability and/or specific activity). In particular, the present invention provides compositions comprising the variant glucoamylases, including starch hydrolyzing compositions, animal feed compositions and cleaning compositions. The invention also relates to DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.
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15.发明申请METHOD OF USING ALPHA-AMYLASE FROM ASPERGILLUS FUMIGATUS AND PULLULANASE FOR SACCHARIFICATION 审中-公开使用来自ASPERGILLUS FUMIGATUS和PULLULANASE的ALPHA-AMYLASE进行鉴定的方法公开(公告)号：US20160040202A1
公开(公告)日：2016-02-11
申请号：US14649170
申请日：2013-12-05
Applicant: DANISCO US INC.
Inventor： Ling Hua , Martijn Scheffers , Marco Van Brussel , Casper Vroemen , Bo Zhang , Zhenghong Zhang
CPC classification number: C12P19/02 , A21D8/042 , A23L2/382 , C11D3/38636 , C12C5/004 , C12C7/04 , C12C7/16 , C12C11/003 , C12C12/04 , C12G3/02 , C12N9/242 , C12P7/14 , C12P19/14 , C12Y302/01001 , C13K1/06 , D06L1/06
Abstract: A fungal alpha-amylase is provided from Aspergillus fumigatus (AfAmy1). AfAmy1 has an optimal pH of 3.5 and is operable at 30-75 degrees C., allowing the enzyme to be used in combination with a glucoamylase and a pullulanase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the alpha-amylase or glucoamylase. AfAmy1 also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DP1+DP2) compared to the products of saccharification catalyzed by an alpha-amylase from Aspergillus kawachii. This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.
Abstract translation: 从烟曲霉（AfAmy1）提供真菌α-淀粉酶。 AfAmy1具有3.5的最佳pH并且在30-75摄氏度下可操作，允许酶在糖化反应中与葡糖淀粉酶和支链淀粉酶组合使用。 这消除了作为分批方法进行糖化反应的必要性，其中必须重新调节pH和温度以最佳地使用α-淀粉酶或葡糖淀粉酶。 与由川曲霉α-淀粉酶催化的糖化产物相比，AfAmy1还催化淀粉底物对显着富含DP2和（DP1 + DP2）的寡糖组合物的糖化。 这有助于例如在同时糖化和发酵过程中由发酵生物体利用寡糖组合物。
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Patent Agency Ranking
16.发明申请METHOD OF USING ALPHA-AMYLASE FROM ASPERGILLUS FUMIGATUS AND ISOAMYLASE FOR SACCHARIFICATION 有权从ASPERGILLUS FUMIGATUS和ISOAMYLASE使用ALPHA-AMYLASE进行鉴定的方法公开(公告)号：US20160032338A1
公开(公告)日：2016-02-04
申请号：US14649173
申请日：2013-12-05
Applicant: DANISCO US INC.
Inventor： Ling Hua , Martijn Scheffers , Marco Van Brussel , Casper Vroemen , Bo Zhang , Zhengong Zhang
CPC classification number: C12P19/14 , A21D8/042 , A23L7/107 , A23V2002/00 , C11D3/386 , C12C5/004 , C12C11/003 , C12N9/242 , C12N9/246 , C12P7/06 , C12P19/02 , C12Y302/01001 , C12Y302/01068 , D06L1/06 , Y02E50/17
Abstract: A fungal alpha-amylase is provided from Aspergillus fumigatus (AfAmyl). AfAmyl has an optimal pH of 3.5 and is operable at 30-75 degrees C., allowing the enzyme to be used in combination with a glucoamylase and an isoamylase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the alpha-amylase or glucoamylase. AfAmyl also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DPI+DP2) compared to the products of saccharification catalyzed by an alpha-amylase from Aspergillus kawachii. This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.
Abstract translation: 真菌α-淀粉酶由烟曲霉（AfAmyl）提供。 AfAmyl具有3.5的最佳pH并且在30-75摄氏度下可操作，允许酶在糖化反应中与葡糖淀粉酶和异淀粉酶组合使用。 这消除了作为分批方法进行糖化反应的必要性，其中必须重新调节pH和温度以最佳地使用α-淀粉酶或葡糖淀粉酶。 与由川崎曲霉α-淀粉酶催化的糖化产物相比，AfAmyl还催化淀粉底物对显着富集DP2和（DPI + DP2）的寡糖组合物的糖化。 这有助于例如在同时糖化和发酵过程中由发酵生物体利用寡糖组合物。
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17.发明申请METHOD OF USING ALPHA-AMYLASE FROM ASPERGILLUS TERREUS AND ISOAMYLASE FOR SACCHARIFICATION 审中-公开从ASPERGILLUS TERREUS和ISOAMYLASE使用ALPHA-AMYLASE进行分类的方法公开(公告)号：US20150376668A1
公开(公告)日：2015-12-31
申请号：US14649805
申请日：2013-12-06
Applicant: DANISCO US INC.
Inventor： Jing Ge , Ling Hua , Martijn Scheffers , Marco Van Brussel , Casper Vroemen , Bo Zhang
CPC classification number: C12P19/14 , A21D8/042 , A23L2/382 , A23L7/107 , A23L29/35 , C11D3/38618 , C11D3/38681 , C12C5/004 , C12N9/242 , C12P7/14 , C12P19/02 , D06L1/14 , D06M16/003
Abstract: A fungal alpha-amylase is provided from Aspergillus terreus (AtAmy1). AtAmy1 has an optimal pH of 4.5 and is operable at 30 75 degrees C., allowing the enzyme to be used in combination with a glucoamylase and an isoamylase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the alpha-amylase or glucoamylase. AtAmy1 also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DP1+DP2) compared to the products of saccharification catalyzed by an alpha amylase from Aspergillus kawachii. This facilitates the utilization of the ligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.
Abstract translation: 从Aspergillus terreus（AtAmy1）提供真菌α-淀粉酶。 AtAmy1具有4.5的最佳pH，并且在30℃下可操作，允许酶在糖化反应中与葡糖淀粉酶和异淀粉酶组合使用。 这消除了作为分批方法进行糖化反应的必要性，其中必须重新调节pH和温度以最佳地使用α-淀粉酶或葡糖淀粉酶。 与由来自曲霉（Aspergillus kawachii）的α淀粉酶催化的糖化产物相比，AtAmy1还催化淀粉底物对显着富集DP2和（DP1 + DP2）的寡糖组合物的糖化。 这有利于在同时糖化和发酵过程中由发酵生物体利用该链状糖组合物。
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公开(公告)号：US09029116B2
公开(公告)日：2015-05-12
申请号：US14015740
申请日：2013-08-30
Applicant: Danisco US Inc.
Inventor： Wolfgang Aehle , Richard R. Bott , Igor Nikoaev , Martijn Scheffers , Piet Van Solingen , Casper Vroemen
CPC classification number: C12N9/2428 , C12N15/09 , C12Y302/01003 , Y02E50/17
Abstract: The present invention relates to glucoamylase variants. In particular, the invention relates to variants in the starch binding domain (SBD) of a glucoamylase. The invention also relates to variants having altered properties (e.g., improved thermostability and/or increased specific activity) as compared to a corresponding parent glucoamylase. The present invention also provides enzyme compositions comprising the variant glucoamylases; DNA constructs comprising polynucleotides encoding the variants; and methods of producing the glucoamylase variants in host cells.
Abstract translation: 本发明涉及葡糖淀粉酶变体。 特别地，本发明涉及葡糖淀粉酶的淀粉结合结构域（SBD）中的变体。 本发明还涉及与相应的母体葡糖淀粉酶相比具有改变的性质（例如，改善的热稳定性和/或增加的比活性）的变体。 本发明还提供了包含变体葡糖淀粉酶的酶组合物; 包含编码变体的多核苷酸的DNA构建体; 以及在宿主细胞中产生葡糖淀粉酶变体的方法。
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19.发明授权公开(公告)号：US08765439B2
公开(公告)日：2014-07-01
申请号：US13656484
申请日：2012-10-19
Applicant: Danisco US Inc.
Inventor： Gang Duan , Kathy Qian , Martijn Scheffers , Jayarama K. Shetty , Pieter Van Solingen
CPC classification number: C12N9/242
Abstract: A maltogenic α-amylase from Trichoderma reesei (TrAA) and variants thereof in the presence of a glucoamylase are useful in the production of high-glucose syrups from liquefied starch, where the high-glucose syrups produced thereby contain at least about 97% glucose. In this process, TrAA advantageously suppresses the reversion of glucose to malto-oligosaccharides. Expression hosts and encoding nucleic acids useful for producing TrAA and its variants also are provided.
Abstract translation: 在葡糖淀粉酶存在下，来自里氏木霉（TrAA）的麦芽糖α-淀粉酶及其变体可用于从液化淀粉生产高葡萄糖浆，其中由此产生的高葡萄糖浆含有至少约97％的葡萄糖。 在此过程中，TrAA有利地抑制了葡萄糖向麦芽寡糖的转化。 还提供了用于产生TrAA及其变体的表达宿主和编码核酸。
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20.发明申请TRICHODERMA REESEI ALPHA-AMYLASE ENHANCES SACCHARIFICATION OF CORN STARCH 失效TRICHODERMA REESEI ALPHA-AMYLASE增强了玉米淀粉的分离公开(公告)号：US20130052302A1
公开(公告)日：2013-02-28
申请号：US13656484
申请日：2012-10-19
Applicant: Danisco US Inc.
Inventor： Gang Duan , Kathy Qian , Martijn Scheffers , Jayarama K. Shetty , Pieter Van Solingen
IPC: A23L1/105
CPC classification number: C12N9/242
Abstract: A maltogenic α-amylase from Trichoderma reesei (TrAA) and variants thereof in the presence of a glucoamylase are useful in the production of high-glucose syrups from liquefied starch, where the high-glucose syrups produced thereby contain at least about 97% glucose. In this process, TrAA advantageously suppresses the reversion of glucose to malto-oligosaccharides. Expression hosts and encoding nucleic acids useful for producing TrAA and its variants also are provided.
Abstract translation: 在葡糖淀粉酶存在下，来自里氏木霉（TrAA）的麦芽糖α-淀粉酶及其变体可用于从液化淀粉生产高葡萄糖浆，其中由此产生的高葡萄糖浆含有至少约97％的葡萄糖。 在此过程中，TrAA有利地抑制了葡萄糖向麦芽寡糖的转化。 还提供了用于产生TrAA及其变体的表达宿主和编码核酸。
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