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    Negatively charged minor groove binders
    15.
    发明申请
    Negatively charged minor groove binders 有权
    带负电荷的小槽粘合剂

    公开(公告)号：US20050187383A1

    公开(公告)日：2005-08-25

    申请号：US10507267

    申请日：2003-03-11

    申请人： Eugeny Lukhtanov ,  Sergey Lokhov ,  Nicolaas Vermeulen

    发明人： Eugeny Lukhtanov ,  Sergey Lokhov ,  Nicolaas Vermeulen

    IPC分类号： C07H21/00 ,  C07H21/04 ,  C12Q1/68

    CPC分类号： C07H21/04 ,  C07H21/00

    摘要： The present invention provides a negatively charged minor groove binding compound, oligonucleotide conjugates comprising the same, and methods for using the same. The negatively charged minor groove binding compounds of the present invention comprises an acidic moiety that is capable of being ionized under physiological conditions. In particular, the negatively charged minor groove binding compound of the present invention comprises a binding moiety that binds preferentially into a minor groove of a double, triple or higher stranded DNA, RNA, PNA or hybrids thereof. The binding moiety comprises at least one aryl moiety and an acidic moiety which is covalently attached to a phenyl portion of the aryl moiety or to a heteroatom of a heteroaryl portion of the aryl moiety.

    摘要翻译： 本发明提供带负电荷的小沟结合化合物，包含其的寡核苷酸缀合物及其使用方法。 本发明的带负电荷的小沟结合化合物包含能够在生理条件下电离的酸性部分。 特别地，本发明的带负电荷的小沟结合化合物包含优先结合双重，三重或更高链DNA，RNA，PNA或其杂交体的小沟的结合部分。 结合部分包含至少一个芳基部分和酸性部分，其共价连接到芳基部分的苯基部分或芳基部分的杂芳基部分的杂原子。

    Hybridization and mismatch discrimination using oligonucleotides conjugated to minor groove binders
    17.
    发明申请
    Hybridization and mismatch discrimination using oligonucleotides conjugated to minor groove binders 审中-公开
    使用与小沟结合物缀合的寡核苷酸的杂交和错配鉴别

    公开(公告)号：US20050064463A1

    公开(公告)日：2005-03-24

    申请号：US10870578

    申请日：2004-06-16

    申请人： Joel Hedgpeth ,  Irina Afonina ,  Igor Kutyavin ,  Eugeny Lukhtanov ,  Evgeniy Belousov ,  Rich Meyer

    发明人： Joel Hedgpeth ,  Irina Afonina ,  Igor Kutyavin ,  Eugeny Lukhtanov ,  Evgeniy Belousov ,  Rich Meyer

    IPC分类号： C12N15/09 ,  C07H19/23 ,  C07H21/00 ,  C07H21/02 ,  C07H21/04 ,  C12Q1/68 ,  C12Q1/6813 ,  G01N21/78 ,  G01N33/483 ,  G01N33/53 ,  G01N33/566 ,  C12P19/34

    CPC分类号： C07H21/00 ,  C07H19/23 ,  C12Q1/6813 ,  C12Q2527/107 ,  C12Q2525/197

    摘要： Conjugates between a minor groove binding molecule, such as the trimer of 1,2-dihydro-(3H)-pyrrolo[3,2-e]indole-7-carboxylate (CDPI3), and an oligonucleotide form unusually stable hybrids with complementary target sequences, in which the tethered CDPI3 group resides in the minor groove of the duplex. These conjugates can be used as probes and primers. Due to their unusually high binding affinity, conjugates as short as 8-mers can be used as amplification primers with high specificity and efficiency. MGB conjugation also increases the discriminatory power of short oligonucleotides, providing enhanced detection of nucleotide sequence mismatches by short oligonucleotides. The MGB-conjugated probes and primers described herein facilitate various analytic and diagnostic procedures, such as amplification reactions, PCR, detection of single-nucleotide polymorphisms, gene hunting, differential display, fluorescence energy transfer, hydrolyzable probe assays and others; by allowing the use of shorter oligonucleotides, which have higher specificity and better discriminatory power.

    摘要翻译： 小沟结合分子（例如1,2-二氢 - （3H） - 吡咯并[3,2-e]吲哚-7-羧酸酯（CDPI3）的三聚体和寡核苷酸之间的缀合物形成非常稳定的具有互补靶的杂交体 序列，其中系留的CDPI3基团位于双链体的小沟槽中。 这些缀合物可以用作探针和引物。 由于其非常高的结合亲和力，短至8聚体的缀合物可以用作高特异性和高效的扩增引物。 MGB缀合还增加了短寡核苷酸的鉴别能力，提供了通过短寡核苷酸增强核苷酸序列错配检测。 本文所述的MGB缀合的探针和引物有助于各种分析和诊断程序，如扩增反应，PCR，单核苷酸多态性检测，基因敲除，差异显示，荧光能量转移，水解性探针测定等。 通过允许使用具有更高特异性和更好的辨别力的较短的寡核苷酸。