公开(公告)号：US20240335480A1

公开(公告)日：2024-10-10

申请号：US18579100

申请日：2022-07-14

Applicant: Astellas Pharma Inc. ,  OSAKA UNIVERSITY ,  National Center for Child Health and Development

Inventor： Kenichiro SHIMATANI ,  Hiromu SATO ,  Masao SASAI ,  Yoshiki SAWA ,  Atsuhiro SAITO ,  Shigeru MIYAGAWA

IPC: A61K35/545 ,  A61K35/44 ,  C12N5/071

CPC classification number: A61K35/545 ,  A61K35/44 ,  C12N5/069 ,  C12N2506/02 ,  C12N2513/00

Abstract: An object of the present invention is to provide a pericyte-like cell having high angiogenic potential with a higher cell proliferation ability than a primary pericyte available in the past and high VEGF expression, and a method for producing the same. Provided are a method for producing a VEGF-highly expressing pericyte-like cell, the method including selecting a CD56(−) pericyte-like cell from a population including a pericyte-like cell obtained by inducing differentiation of a pluripotent stem cell; and a VEGF-highly expressing pericyte-like cell produced by the production method.

公开(公告)号：US20240157164A1

公开(公告)日：2024-05-16

申请号：US18284467

申请日：2022-03-25

Applicant: OSAKA UNIVERSITY

Inventor： Masakazu YAGI ,  Yoshiki SAWA ,  Hirotada MASUDA ,  Kotaro YOSHIDA ,  Fumito MINAKAMI ,  Eri KANEDA

IPC: A61N2/00 ,  A61N2/06

CPC classification number: A61N2/006 ,  A61N2/06

Abstract: In order to provide a technical means that offers excellent convenience, usability, and safety for stellate ganglion block that can effectively suppress sympathetic overexcitation, this stellate ganglion magnetic stimulation device comprises: a head unit having a magnet for generating magnetism; and a neck unit that is removably worn around a wearer's neck and that presses the head unit against epidermis corresponding to the stellate ganglion so that stimulation by the magnetism acts on the stellate ganglion.

公开(公告)号：US20240122533A1

公开(公告)日：2024-04-18

申请号：US18546448

申请日：2021-11-15

Applicant: OSAKA UNIVERSITY ,  HEARTLAB, INC.

Inventor： Shigeru MIYAGAWA ,  Yoshiki SAWA ,  Hidetsugu ASANOI ,  Sunao IKEGAWA

IPC: A61B5/00 ,  A61B5/08

CPC classification number: A61B5/4842 ,  A61B5/0816 ,  A61B5/6892 ,  A61B2562/0247

Abstract: The present invention relates to an evaluation device 4 for evaluating the severity of pneumonia in a target patient, comprising: an acquisition unit 42 for acquiring a respiratory waveform of the target patient; a calculation unit 43 for calculating a value of an index indicating instability of a respiratory cycle or a respiratory frequency from the respiratory waveform; and an evaluation unit 44 for evaluating the severity based on the calculated value.

公开(公告)号：US20230414192A1

公开(公告)日：2023-12-28

申请号：US18463894

申请日：2023-09-08

Applicant: OSAKA UNIVERSITY

Inventor： Hirotada MASUDA ,  Yoshiki SAWA ,  Toru KURATANI

IPC: A61B7/04 ,  A61B5/00 ,  A61B5/02

CPC classification number: A61B7/04 ,  A61B5/7275 ,  A61B5/02028 ,  A61B5/02007 ,  A61B5/726

Abstract: A heart valve abnormality detection device includes a heart sound data acquisition portion 21 configured to acquire heart sound data corresponding to heart sounds, a first processing portion configured to set, based on the heart sound data, a section between a first heart sound and a second heart sound of the heart sounds as a target range and acquire a peak frequency that is a peak value of a frequency in a frequency component of the heart sounds within the target range, and a second processing portion configured to output a detection signal indicating that there is a high probability of severe aortic stenosis in a case where the peak frequency is a peak reference value or greater.

公开(公告)号：US20180153155A1

公开(公告)日：2018-06-07

申请号：US15872157

申请日：2018-01-16

Applicant: TERUMO KABUSHIKI KAISHA ,  OSAKA UNIVERSITY

Inventor： Fumiya OHASHI ,  Shigeru MIYAGAWA ,  Yoshiki SAWA ,  Shigeo MASUDA ,  Satsuki FUKUSHIMA ,  Atsuhiro SAITO

IPC: A01N1/02 ,  G01N33/50 ,  C12N5/077 ,  A61K35/545

CPC classification number: A01N1/0215 ,  A01N1/0221 ,  A61K35/28 ,  A61K35/34 ,  A61K35/545 ,  C12N5/0653 ,  C12N5/0657 ,  C12N5/10 ,  G01N33/5073

Abstract: A method is disclosed for cryopreservation of cardiocytes derived from pluripotent stem cells or mesenchymal stem cells derived from adipose tissue or bone marrow, the method maintaining the function of the cardiocytes derived from differentiated pluripotent stem cells or mesenchymal stem cells derived from adipose tissue or bone marrow, and yet reducing the possibility for tumorigenesis of undifferentiated pluripotent stem cells or mesenchymal stem cells derived from adipose tissue or bone marrow. A method is also disclosed for cryopreservation of cardiocytes derived from pluripotent stem cells or mesenchymal stem cells (derived from adipose tissue or bone marrow, the method including dissociating cells from a cell population which has been induced to differentiate into cardiocytes from pluripotent stem cells or mesenchymal stem cells derived from adipose tissue or bone marrow.

公开(公告)号：US20240182858A1

公开(公告)日：2024-06-06

申请号：US18282450

申请日：2022-03-16

Applicant: Astellas Pharma Inc. ,  Osaka University

Inventor： Kenichiro SHIMATANI ,  Hiromu SATO ,  Masao SASAI ,  Yoshiki SAWA ,  Atsuhiro SAITO ,  Shigeru MIYAGAWA

IPC: C12N5/077 ,  A61K35/44 ,  A61P7/00 ,  C07K14/50 ,  C12N5/10

CPC classification number: C12N5/0661 ,  A61K35/44 ,  A61P7/00 ,  C07K14/503 ,  C12N5/10

Abstract: An object of the present invention is to provide a cell therapy that is expected to be useful as an angiogenic therapy for a peripheral vascular disease such as critical lower limb ischemia. Specifically, the object is to provide a pericyte having high angiogenic potential and a method for producing the same. A pericyte introduced a bFGF gene and a method for producing the same are provided.

公开(公告)号：US20220372437A1

公开(公告)日：2022-11-24

申请号：US17765637

申请日：2020-09-09

Applicant: OSAKA UNIVERSITY

Inventor： Takaaki SAMURA ,  Shigeru MIYAGAWA ,  Yoshiki SAWA ,  Junya YOKOYAMA

IPC: C12N5/00 ,  C07K14/75

Abstract: A method for producing a fibrin sheet containing at least one selected from the group consisting of cells and drugs in a fibrin gel, the method comprising: a step 1 of applying a fibrinogen solution containing at least one selected from the group consisting of cells and drugs and fibrinogen dropwise onto a surface of a substrate made of a gelatin hydrogel; a step 2 of adding thrombin to the fibrinogen solution on the surface of the substrate; a step 3 of placing a support film on and in contact with a top surface of the fibrinogen solution to which the thrombin has been added; a step 4 of forming a fibrin sheet containing the at least one selected from the group consisting of cells and drugs in a fibrin gel between the substrate and the support film by a reaction between the fibrinogen and the thrombin; and a step 5 of melting the substrate at a temperature not lower than a melting temperature of the gelatin hydrogel to separate, from the substrate, the fibrin sheet supported by the support film.

公开(公告)号：US20170247658A1

公开(公告)日：2017-08-31

申请号：US15594011

申请日：2017-05-12

Applicant: TERUMO KABUSHIKI KAISHA ,  OSAKA UNIVERSITY

Inventor： Hiroko ISEOKA ,  Yoshiki SAWA ,  Shigeru MIYAGAWA ,  Satsuki FUKUSHIMA

IPC: C12N5/077 ,  A61K35/34

CPC classification number: C12N5/0657 ,  A61K35/12 ,  A61K35/34 ,  A61L27/00 ,  C12N2506/45

Abstract: A sheet-shaped cell culture is disclosed that includes myocardial cells derived from pluripotent stem cells and has excellent functional properties. A sheet-shaped cell culture obtained by culturing a cell population that includes myocardial cells derived from pluripotent stem cells, wherein the proportion of the number of the myocardial cells derived from pluripotent stem cells to the total number of cells in the cell population is 50% to 70%; a method of producing the sheet-shaped cell culture; a composition including the sheet-shaped cell culture; and a method of treating a disease using the sheet-shaped cell culture or the composition.

公开(公告)号：US20170035939A1

公开(公告)日：2017-02-09

申请号：US15291408

申请日：2016-10-12

Applicant: CELLSEED INC. ,  OSAKA UNIVERSITY

Inventor： Yukiko IMANISHI ,  Yoshiki SAWA ,  Iichiro SHIMOMURA ,  Norikazu MAEDA ,  Shigeru MIYAGAWA ,  Hideaki SAKAI

IPC: A61L27/38 ,  C12N5/077 ,  A61L27/36

CPC classification number: A61L27/3804 ,  A61K35/35 ,  A61L27/3683 ,  A61L27/3691 ,  A61L27/3695 ,  A61L27/3839 ,  A61L27/3891 ,  A61L2300/64 ,  A61L2430/20 ,  C12N5/0653 ,  C12N2500/38 ,  C12N2501/33 ,  C12N2501/39 ,  C12N2539/10

Abstract: The present invention aims to provide an artificial tissue that can efficiently reproduce myocardial tissue function and that can be used in an actual implantation and produced by culturing. The present invention relates to a graft material for treating myocardial disease, the graft material including a cell sheet containing adipocytes.

Abstract translation: 本发明的目的在于提供能够有效地再现心肌组织功能的人造组织，其可以用于实际植入中并通过培养产生。 本发明涉及用于治疗心肌疾病的移植材料，该移植材料包括含有脂肪细胞的细胞片。

公开(公告)号：US20160058908A1

公开(公告)日：2016-03-03

申请号：US14844259

申请日：2015-09-03

Applicant: TERUMO KABUSHIKI KAISHA ,  OSAKA UNIVERSITY

Inventor： Fumiya OOHASHI ,  Ryouhei TAKEUCHI ,  Tadashi SAMESHIMA ,  Shigeru MIYAGAWA ,  Yoshiki SAWA ,  Mitsuhiro SAITO

IPC: A61L27/34 ,  A61L27/58 ,  A61L27/38

CPC classification number: A61L27/58 ,  A61L27/225 ,  A61L27/38 ,  A61L27/52 ,  A61L2430/20

Abstract: A laminate of a sheet-shaped cell culture is disclosed, which has excellent operability and is suitable for implantation. A method is disclosed for producing a laminate of a fibrin gel and a sheet-shaped cell culture, including a step of dripping a liquid containing fibrinogen onto an upper surface of a sheet-shaped cell culture, a step of spraying a liquid containing thrombin onto the surface, and a step of forming a fibrin gel layer on the surface by a reaction between fibrinogen and thrombin; and a laminate of a fibrin gel and a sheet-shaped cell culture produced by the method are disclosed.

Abstract translation: 公开了一种片状细胞培养物的层压体，其具有优异的可操作性并且适于植入。 公开了用于制造纤维蛋白凝胶和片状细胞培养物的层压体的方法，包括将含有纤维蛋白原的液体滴落到片状细胞培养物的上表面的步骤，将含有凝血酶的液体喷雾到 表面和通过纤维蛋白原和凝血酶之间的反应在表面上形成纤维蛋白凝胶层的步骤; 并且公开了通过该方法制备的纤维蛋白凝胶和片状细胞培养物的层压体。