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公开(公告)号：US07425337B2
公开(公告)日：2008-09-16
申请号：US11376901
申请日：2006-03-16
CPC分类号： C12N7/00 , C12N15/86 , C12N2770/36143 , C12N2770/36152 , C12N2770/36162 , C12N2840/203 , C12N2840/206
摘要： The present invention provides compositions useful in and methods for producing populations of infectious, replication-defective alphavirus replicon particles that contain no replication competent alphavirus particles, as determined by passage on cells in culture. The compositions include helper and replicon nucleic acid molecules that can further reduce the predicted frequency for formation of replication-competent virus and can optimize manufacturing strategies and costs.
摘要翻译： 本发明提供了可用于生产感染性，复制缺陷型甲病毒复制子颗粒群体的组合物，所述复制子颗粒不含复制受感染的甲病毒颗粒，如通过培养细胞传代所确定的。 组合物包括辅助和复制子核酸分子，其可以进一步减少用于形成具有复制能力的病毒的预测频率，并且可以优化制造策略和成本。
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公开(公告)号：US07045335B2
公开(公告)日：2006-05-16
申请号：US10237302
申请日：2002-09-06
CPC分类号： C12N7/00 , C12N15/86 , C12N2770/36143 , C12N2770/36152 , C12N2770/36162 , C12N2840/203 , C12N2840/206
摘要： The present invention provides compositions useful in and methods for producing populations of infectious, replication-defective alphavirus replicon particles that contain no replication-competent alphavirus particles, as determined by passage on cells in culture. The compositions include helper and replicon nucleic acid molecules that can further reduce the predicted frequency for formation of replication-competent virus and can optimize manufacturing strategies and costs.
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13.发明授权公开(公告)号：US06451592B1
公开(公告)日：2002-09-17
申请号：US08944465
申请日：1997-10-06
申请人： Thomas W. Dubensky, Jr. , John M. Polo , Barbara A. Belli , Sondra Schlesinger , Sergey A. Dryga , Ilya Frolov
发明人： Thomas W. Dubensky, Jr. , John M. Polo , Barbara A. Belli , Sondra Schlesinger , Sergey A. Dryga , Ilya Frolov
IPC分类号： C12N1500
CPC分类号： C07K14/005 , A61K39/00 , A61K48/00 , C12N7/00 , C12N15/79 , C12N15/86 , C12N2770/36121 , C12N2770/36122 , C12N2770/36143 , C12N2770/36162 , C12N2830/005 , C12N2830/006 , C12N2830/42 , C12N2830/48 , C12N2840/20 , C12N2840/203 , C12N2840/44
摘要： Isolated nucleic acid molecules are disclosed, comprising an alphavirus nonstructural protein gene which, when operably incorporated into a recombinant alphavirus particle, eukaryotic layered vector initiation system, or RNA vector replicon, has a reduced level of vector-specific RNA synthesis, as compared to wild-type, and the same or greater level of proteins encoded by RNA transcribed from the viral junction region promoter, as compared to a wild-type recombinant alphavirus particle. Also disclosed are RNA vector replicons, alphavirus vector constructs, and eukaryotic layered vector initiation systems which contain the above-identified nucleic acid molecules.
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公开(公告)号：US20110262933A1
公开(公告)日：2011-10-27
申请号：US13091527
申请日：2011-04-21
IPC分类号： G01N33/569 , C12N1/20 , C07K1/107 , C07K16/12
CPC分类号： G01N33/54326 , A61B5/055 , B01L3/502761 , B01L2200/0647 , B01L2400/043 , C07K1/22 , C07K16/1267 , C12N13/00 , C12N15/1013 , C12Q1/6806 , C12Q1/689 , C12Q2565/633 , G01N24/08 , G01N33/54333 , G01N33/56911 , G01N2333/195 , G01N2446/20 , G01R33/465 , Y10T428/2982 , C12Q2565/629 , C12Q2563/143
摘要： The invention generally relates to compositions and methods for isolating mid-log phase bacteria. In certain embodiments, the invention provides a magnetic particle conjugated to an antibody that includes an epitope specific for mid-log phase bacteria.
摘要翻译： 本发明一般涉及用于分离对数中期细菌的组合物和方法。 在某些实施方案中，本发明提供了与抗体缀合的磁性颗粒，其包括对数中期细菌特异性的表位。
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公开(公告)号：US20110262927A1
公开(公告)日：2011-10-27
申请号：US13091548
申请日：2011-04-21
CPC分类号： G01N33/54326 , A61B5/055 , B01L3/502761 , B01L2200/0647 , B01L2400/043 , C07K1/22 , C07K16/1267 , C12N13/00 , C12N15/1013 , C12Q1/6806 , C12Q1/689 , C12Q2565/633 , G01N24/08 , G01N33/54333 , G01N33/56911 , G01N2333/195 , G01N2446/20 , G01R33/465 , Y10T428/2982 , C12Q2565/629 , C12Q2563/143
摘要： The invention generally relates to conducting an assay on a sample that isolates a pathogen from the sample and allows for analysis of the pathogen with minimal (i.e., at most 24 hrs of culturing) or no culturing of the pathogen. In certain embodiments, the invention provides methods for identifying a pathogen from a sample that involve obtaining a sample including a pathogen, conducting an assay that isolates the pathogen from the sample, culturing the isolated pathogen for at most about 24 hrs, and analyzing the pathogen.
摘要翻译： 本发明一般涉及对从样品中分离病原体的样品进行测定，并允许以最小（即培养最多24小时）或不培养病原体来分析病原体。 在某些实施方案中，本发明提供了从样品中鉴定病原体的方法，所述病原体涉及获得包含病原体的样品，进行将病原体与样品分离的培养物，培养分离的病原体至多约24小时，并分析病原体 。
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公开(公告)号：US20110262893A1
公开(公告)日：2011-10-27
申请号：US12855147
申请日：2010-08-12
IPC分类号： G01N33/569 , C12Q1/68 , C12N13/00 , C07K16/00 , G01N33/566 , C07K1/14 , C07H1/06 , C12Q1/70 , G01N33/567
CPC分类号： G01N33/54326 , A61B5/055 , B01L3/502761 , B01L2200/0647 , B01L2400/043 , C07K1/22 , C07K16/1267 , C12N13/00 , C12N15/1013 , C12Q1/6806 , C12Q1/689 , C12Q2565/633 , G01N24/08 , G01N33/54333 , G01N33/56911 , G01N2333/195 , G01N2446/20 , G01R33/465 , Y10T428/2982 , C12Q2565/629 , C12Q2563/143
摘要： The invention generally relates to using magnetic particles and alternating magnet fields to separate a target analyte from a sample. In certain embodiments, methods of the invention involve contacting a sample with magnetic particles including first moieties specific for a target analyte, thereby forming target/particle complexes in the sample, flowing the sample through a channel including second moieties attached to at least one surface of the channel, applying alternating magnetic fields to the flowing sample to result in target/particle complexes being brought into proximity of the surface to bind the second moieties and unbound particles remaining free in the sample, binding the target/particle complexes to the second moieties, and washing away unbound particles and unbound analytes of the sample.
摘要翻译： 本发明一般涉及使用磁性颗粒和交替磁场将目标分析物与样品分离。 在某些实施方案中，本发明的方法包括将样品与包括对于目标分析物特异性的第一部分的磁性颗粒接触，从而在样品中形成靶/颗粒复合物，使样品流过包含连接至至少一个表面的第二部分的通道 通道，向流动的样品施加交变磁场以导致靶/颗粒复合物接近表面以结合样品中残留的第二部分和未结合的颗粒，将靶/颗粒复合物结合到第二部分， 并清洗样品中未结合的颗粒和未结合的分析物。
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公开(公告)号：US08841104B2
公开(公告)日：2014-09-23
申请号：US13091510
申请日：2011-04-21
申请人： Sergey A. Dryga , Victor C. Esch , Lisa-Jo Ann Clarizia , Eddie W. Adams , Thearith H. Ung , Ravil A. Sitdikov
发明人： Sergey A. Dryga , Victor C. Esch , Lisa-Jo Ann Clarizia , Eddie W. Adams , Thearith H. Ung , Ravil A. Sitdikov
IPC分类号： C12Q1/24 , G01N33/563 , G01N33/543 , C12Q1/68 , C07K16/12 , C12N13/00 , C07K1/22
CPC分类号： G01N33/5434 , B01L3/502738 , B01L2300/0636 , C07K1/22 , C07K16/1267 , C12N13/00 , C12Q1/68 , C12Q1/6806 , G01N21/64 , G01N33/53 , G01N33/54333 , G01N2446/90 , Y10T436/25 , Y10T436/25375 , C12Q2565/629 , C12Q2563/143
摘要： The invention generally relates to methods of using compositions that include sets of magnetic particles, members of each set being conjugated to an antibody specific for a pathogen, and magnets to isolate a pathogen from a body fluid sample.
摘要翻译： 本发明一般涉及使用组合物的方法，所述组合物包括一组磁性颗粒，每组的成员与特异于病原体的抗体缀合，以及磁体，用于将病原体与体液样品分离。
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公开(公告)号：US20110262989A1
公开(公告)日：2011-10-27
申请号：US12850203
申请日：2010-08-04
IPC分类号： C12N13/00
CPC分类号： G01N33/54326 , A61B5/055 , B01L3/502761 , B01L2200/0647 , B01L2400/043 , C07K1/22 , C07K16/1267 , C12N13/00 , C12N15/1013 , C12Q1/6806 , C12Q1/689 , C12Q2565/633 , G01N24/08 , G01N33/54333 , G01N33/56911 , G01N2333/195 , G01N2446/20 , G01R33/465 , Y10T428/2982 , C12Q2565/629 , C12Q2563/143
摘要： The invention generally relates to using magnetic particles and magnets to isolate a target analyte from a body fluid sample. In certain embodiments, methods of the invention involve introducing magnetic particles including a target-specific binding moiety to a body fluid sample in order to create a mixture, incubating the mixture to allow the particles to bind to a target, applying a magnetic field to capture target/magnetic particle complexes on a surface, and washing with a wash solution that reduces particle aggregation, thereby isolating target/magnetic particle complexes.
摘要翻译： 本发明一般涉及使用磁性颗粒和磁体将目标分析物与体液样品分离。 在某些实施方案中，本发明的方法包括将包含靶特异性结合部分的磁性颗粒引入体液样品以产生混合物，将混合物孵育以允许颗粒结合靶，施加磁场以捕获 表面上的靶/磁性颗粒复合物，并且用洗涤溶液洗涤以减少颗粒聚集，从而分离靶/磁性颗粒复合物。
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公开(公告)号：US20110262926A1
公开(公告)日：2011-10-27
申请号：US13091534
申请日：2011-04-21
IPC分类号： C12Q1/02 , G01N33/569 , G01N27/00 , C12Q1/04 , C12Q1/68
CPC分类号： G01N33/54326 , A61B5/055 , B01L3/502761 , B01L2200/0647 , B01L2400/043 , C07K1/22 , C07K16/1267 , C12N13/00 , C12N15/1013 , C12Q1/6806 , C12Q1/689 , C12Q2565/633 , G01N24/08 , G01N33/54333 , G01N33/56911 , G01N2333/195 , G01N2446/20 , G01R33/465 , Y10T428/2982 , C12Q2565/629 , C12Q2563/143
摘要： The invention generally relates to conducting an assay on a sample that isolates a bacterium from the sample, in which the assay isolates as low as about 1 CFU/ml of bacteria in the sample.
摘要翻译： 本发明一般涉及对从样品中分离出细菌的样品进行测定，其中测定分离出样品中低至约1CFU / ml的细菌。
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20.发明授权公开(公告)号：US06465634B1
公开(公告)日：2002-10-15
申请号：US09415900
申请日：1999-10-08
申请人： Thomas W. Dubensky, Jr. , John M. Polo , Barbara A. Belli , Sondra Schlesinger , Sergey A. Dryga , Ilya Frolov
发明人： Thomas W. Dubensky, Jr. , John M. Polo , Barbara A. Belli , Sondra Schlesinger , Sergey A. Dryga , Ilya Frolov
IPC分类号： C07H2104
CPC分类号： C07K14/005 , A61K39/00 , A61K48/00 , C12N7/00 , C12N15/86 , C12N2770/36121 , C12N2770/36122 , C12N2770/36143 , C12N2770/36162 , C12N2830/005 , C12N2830/006 , C12N2830/42 , C12N2830/48 , C12N2840/20 , C12N2840/203 , C12N2840/44
摘要： Isolated nucleic acid molecules are disclosed, comprising an alphavirus nonstructural protein gene which, when operably incorporated into a recombinant alphavirus particle, eukaryotic layered vector initiation system, or RNA vector replicon, has a reduced level of vector-specific RNA synthesis, as compared to wild-type, and the same or greater level of proteins encoded by RNA transcribed from the viral junction region promoter, as compared to a wild-type recombinant alphavirus particle. Also disclosed are RNA vector replicons, alphavirus vector constructs, and eukaryotic layered vector initiation systems which contain the above-identified nucleic acid molecules.
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