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公开(公告)号：US08685248B2
公开(公告)日：2014-04-01
申请号：US12709556
申请日：2010-02-22
IPC分类号： B01D15/08
CPC分类号： B01J20/262 , B01J20/267 , B01J20/28019 , B01J20/28023 , B01J20/28033 , B01J20/28042 , B01J20/286 , B01J20/289 , B01J20/3092 , B01J20/3208 , B01J20/3212 , B01J20/3219 , B01J20/3246 , B01J20/3248 , B01J20/3251 , B01J20/3255 , B01J20/3272 , B01J20/3285 , B01J2220/54 , B01J2220/58 , C07K16/065 , Y10T428/249963
摘要： The present invention relates to a separation matrix comprised of a porous support to which ligands have been immobilized, wherein said ligands comprise at least one aliphatic sulphonamide. The nitrogen of the sulphonamide may be a secondary or tertiary amine. The invention also relates to a chromatography column that contains the described separation matrix, as well as to a method of isolating immunoglobulin-like compounds by adsorption to a separation matrix that comprises aliphatic sulphonamide ligands.
摘要翻译： 本发明涉及由已经固定有配体的多孔载体组成的分离基质，其中所述配体包含至少一种脂族磺酰胺。 磺酰胺的氮可以是仲胺或叔胺。 本发明还涉及含有所述分离基质的色谱柱，以及通过吸附到包含脂族磺酰胺配体的分离基质上分离免疫球蛋白样化合物的方法。
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公开(公告)号：US08372286B2
公开(公告)日：2013-02-12
申请号：US12863448
申请日：2009-01-30
IPC分类号： B01D15/08
CPC分类号： B01J20/291 , B01J20/262 , B01J20/2809 , B01J20/285 , B01J20/286 , B01J20/287 , B01J20/288 , B01J20/3212 , B01J20/3268 , B01J20/3274 , B01J20/3293 , B01J39/26 , B01J41/20 , B01J2220/52
摘要： The present invention relates to a method for production of separation media using a so called Spinning Disc technology wherein the porosities of the beads are optimized in such a way that a desired biomolecule may be separated from a complex sample. The method comprises the following steps: a) feeding a 4-8% polysaccharide solution, which has a viscosity within 350-450 mPas, at 65-75° C. to one or more spinning discs at 3001-3010 rpm to form polysaccharide beads; b) capturing said formed polysaccharide beads in a capturing bath; wherein the porosity of the polysaccharide beads is controlled by varying the temperature of the capturing between 15 and 27° C., preferably between 17.5 and 24.6° C. The method yields porosities that prevent molecules larger than 150 000 g/mol to diffuse into the beads. The invention also relates to separation media produced by the method and use thereof for purification of biomolecules, in particular monoclonal antibodies.
摘要翻译： 本发明涉及使用所谓的旋转圆盘技术生产分离介质的方法，其中珠子的孔隙度以使得所需生物分子可以与复杂样品分离的方式进行优化。 该方法包括以下步骤：a）将具有350-450mPa·s粘度的4-8％多糖溶液在65-75℃下以3001-3010rpm向一个或多个纺丝盘送入，以形成多糖珠粒 ; b）在捕获浴中捕获所述形成的多糖珠粒; 其中多糖珠的孔隙率通过在15和27℃之间，优选在17.5和24.6℃之间改变捕获温度来控制。该方法产生阻止大于150000g / mol的分子扩散到 珠子 本发明还涉及通过其用于纯化生物分子，特别是单克隆抗体的方法和用途产生的分离培养基。
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公开(公告)号：US08114280B2
公开(公告)日：2012-02-14
申请号：US11570530
申请日：2005-06-22
IPC分类号： B01D15/08
CPC分类号： B01J20/3212 , B01D15/426 , B01J20/286 , B01J20/289 , B01J20/3219 , B01J20/3251
摘要： The present invention relates to a separation matrix comprised of a porous support to which ligands have been immobilized, wherein said ligands comprise at least one sulphonamide and the R group of the sulphonyl comprises an aromatic group. The nitrogen of the sulphonamide may be a secondary or tertiary amine. The invention also relates to a chromatography column that contains the described separation matrix, as well as to a method of isolating immunoglobulin-like compounds by adsorption to the separation matrix.
摘要翻译： 本发明涉及由已经固定有配体的多孔载体组成的分离基质，其中所述配体包含至少一个磺酰胺，并且磺酰基的R基包括芳族基团。 磺酰胺的氮可以是仲胺或叔胺。 本发明还涉及含有所述分离基质的色谱柱，以及通过吸附到分离基质上分离免疫球蛋白样化合物的方法。
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公开(公告)号：US20100298548A1
公开(公告)日：2010-11-25
申请号：US12863448
申请日：2009-01-30
CPC分类号： B01J20/291 , B01J20/262 , B01J20/2809 , B01J20/285 , B01J20/286 , B01J20/287 , B01J20/288 , B01J20/3212 , B01J20/3268 , B01J20/3274 , B01J20/3293 , B01J39/26 , B01J41/20 , B01J2220/52
摘要： The present invention relates to a method for production of separation media using a so called Spinning Disc technology wherein the porosities of the beads are optimized in such a way that a desired biomolecule may be separated from a complex sample. The method comprises the following steps: a) feeding a 4-8% polysaccharide solution, which has a viscosity within 350-450 mPas, at 65-75° C. to one or more spinning discs at 3001-3010 rpm to form polysaccharide beads; b) capturing said formed polysaccharide beads in a capturing bath; wherein the porosity of the polysaccharide beads is controlled by varying the temperature of the capturing between 15 and 27° C., preferably between 17.5 and 24.6° C. The method yields porosities that prevent molecules larger than 150 000 g/mol to diffuse into the beads. The invention also relates to separation media produced by the method and use thereof for purification of biomolecules, in particular monoclonal antibodies.
摘要翻译： 本发明涉及使用所谓的旋转圆盘技术生产分离介质的方法，其中珠子的孔隙度以使得所需生物分子可以与复杂样品分离的方式进行优化。 该方法包括以下步骤：a）将具有350-450mPa·s粘度的4-8％多糖溶液在65-75℃下以3001-3010rpm向一个或多个纺丝盘送入，以形成多糖珠 ; b）在捕获浴中捕获所述形成的多糖珠粒; 其中多糖珠的孔隙率通过在15和27℃之间，优选在17.5和24.6℃之间改变捕获温度来控制。该方法产生阻止大于150000g / mol的分子扩散到 珠子 本发明还涉及通过其用于纯化生物分子，特别是单克隆抗体的方法和用途产生的分离培养基。
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15.发明申请METHOD OF PREPARING AN IMMOBILISED METAL ION CHROMATOGRAPHY ADSORBENT AND METHODS OF PURIFYING PROTEINS, PEPTIDES OR POLYNUCLEOTIDES 审中-公开制备固定化金属离子色谱吸附剂的方法和净化蛋白质，肽或多核苷酸的方法公开(公告)号：US20100016564A1
公开(公告)日：2010-01-21
申请号：US12297241
申请日：2007-05-28
申请人： Lars C. Andersson , Makonnen Belew , Tesfai Gebru , Gunnar Glad , Helena Lindgren , Nils Norrman
发明人： Lars C. Andersson , Makonnen Belew , Tesfai Gebru , Gunnar Glad , Helena Lindgren , Nils Norrman
CPC分类号： B01J20/3265 , B01D15/3828 , B01J20/289 , B01J45/00
摘要： The present invention relates to a method of preparing an immobilised metal ion affinity chromatography (IMAC) adsorbent, which comprises to provide chromatography ligands comprised of alkylene diamine triacetic acid, or a derivative thereof, and coupling thereof to a carrier via nitrogen. In an advantageous embodiment, the alkylene diamine triacetic acid is ethylene diaminetriacetic acid (ED3A).
摘要翻译： 本发明涉及一种制备固定金属离子亲和层析（IMAC）吸附剂的方法，其包括提供由亚烷基二胺三乙酸或其衍生物组成的色谱配体，以及通过氮与载体的偶联。 在有利的实施方案中，亚烷基二胺三乙酸是乙二胺三乙酸（ED3A）。
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公开(公告)号：US20080249289A1
公开(公告)日：2008-10-09
申请号：US12093229
申请日：2006-11-16
申请人： Andreas Axen , Gunnar Glad , Henrik Ihre , Anders Larsson , Nils Norrman
发明人： Andreas Axen , Gunnar Glad , Henrik Ihre , Anders Larsson , Nils Norrman
CPC分类号： B01J20/289 , B01J20/262 , B01J20/265 , B01J20/267 , B01J20/285 , B01J20/3242 , B01J20/3244 , B01J39/26
摘要： The present invention relates to method of manufacturing a sulphonate-functionalized (S-functionalized) cation exchanger, which method comprises reacting olefinic groups with bisulphite in the presence of at least one amine oxide, such as N-methyl morpholine oxide (NMO). The olefinic groups of the carrier may be allyl groups; which may be provided on extenders such as polyhydroxyfunctional polymers. In one embodiment, the carrier is made of agarose with improved flow pressure properties.
摘要翻译： 本发明涉及制备磺酸酯官能化（S-官能化）阳离子交换剂的方法，该方法包括在至少一种氧化胺如N-甲基吗啉氧化物（NMO）存在下使烯基与亚硫酸氢盐反应。 载体的烯基可以是烯丙基; 其可以在增量剂如多羟基官能聚合物上提供。 在一个实施方案中，载体由具有改进的流动压力性质的琼脂糖制成。
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公开(公告)号：US08858800B2
公开(公告)日：2014-10-14
申请号：US11996357
申请日：2006-08-30
申请人： Andreas Axen , Gunnar Glad , Henrik Ihre , Anders Larsson , Nils Norrman
发明人： Andreas Axen , Gunnar Glad , Henrik Ihre , Anders Larsson , Nils Norrman
IPC分类号： B01D15/36 , B01J39/26 , B01J20/289 , B01J39/18
CPC分类号： B01J39/26 , B01D15/362 , B01J20/289 , B01J39/19
摘要： The present invention relates to a method of manufacturing a chromatography matrix comprising providing a polysaccharide carrier comprising available hydroxyl groups; and reacting said hydroxyl groups with vinyl sulphonate to provide a sulphonate-functionalized (S-functionalized) cation exchanger. The hydroxyl groups of the carrier may be hydroxyls of the agarose polymer; or alternatively they may be provided on extenders such as polyhydroxyfunctional polymers. In one embodiment, the carrier is made of agarose with improved flow pressure properties.
摘要翻译： 本发明涉及一种制备色谱基质的方法，包括提供包含可用羟基的多糖载体; 并使所述羟基与乙烯基磺酸酯反应以提供磺酸盐官能化的（S-官能化的）阳离子交换剂。 载体的羟基可以是琼脂糖聚合物的羟基; 或者它们可以提供在增量剂上，例如多羟基官能聚合物。 在一个实施方案中，载体由具有改进的流动压力性质的琼脂糖制成。
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公开(公告)号：US20110155668A1
公开(公告)日：2011-06-30
申请号：US13002878
申请日：2009-06-04
CPC分类号： B01D15/3828 , B01D15/166 , B01D15/36 , B01J20/28092 , B01J20/285 , B01J20/286 , B01J20/3242 , B01J20/3293 , C07K1/16 , C07K1/22 , C07K16/00
摘要： The present invention relates to a separation medium, comprising an inner core of a porous material provided with charged ligands, and an outer lid comprising a porous material provided with charged ligands, wherein the charge of the ligands in the inner core is opposite that of the charge of the ligands in the lid. The present invention also relates to a method for biomolecule separation comprising applying a sample to the above separation medium, wherein large molecules are prevented from entering the medium by charge repulsion from the medium and small molecules are captured in the inner core.
摘要翻译： 本发明涉及一种分离介质，其包括具有带电配位体的多孔材料的内核和包含带有带电配位体的多孔材料的外盖，其中内芯中配体的电荷与 盖中配体的电荷。 本发明还涉及一种用于生物分子分离的方法，包括将样品施加到上述分离介质中，其中通过从介质的电荷排斥来防止大分子进入介质，并且小分子被捕获在内核中。
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公开(公告)号：US20070196858A1
公开(公告)日：2007-08-23
申请号：US11570530
申请日：2005-06-22
CPC分类号： B01J20/3212 , B01D15/426 , B01J20/286 , B01J20/289 , B01J20/3219 , B01J20/3251
摘要： The present invention relates to a separation matrix comprised of a porous support to which ligands have been immobilised, wherein said ligands comprise at least one sulphonamide and the R group of the sulphonyl comprises an aromatic group. The nitrogen of the sulphonamide may be a secondary or tertiary amine. The invention also relates to a chromatography column that contains the described separation matrix, as well as to a method of isolating immunoglobulin-like compounds by adsorption to the separation matrix.
摘要翻译： 本发明涉及由已经固定有配体的多孔载体组成的分离基质，其中所述配体包含至少一个磺酰胺，并且磺酰基的R基包括芳族基团。 磺酰胺的氮可以是仲胺或叔胺。 本发明还涉及含有所述分离基质的色谱柱，以及通过吸附到分离基质上分离免疫球蛋白样化合物的方法。
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公开(公告)号：USD709210S1
公开(公告)日：2014-07-15
申请号：US29402999
申请日：2011-09-30
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