利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

48 条记录 (耗时 0.028 秒)

    Separation Matrix And Method Of Purification
    3.
    发明申请
    Separation Matrix And Method Of Purification 有权
    分离矩阵和纯化方法

    公开(公告)号：US20070196858A1

    公开(公告)日：2007-08-23

    申请号：US11570530

    申请日：2005-06-22

    申请人： Gunnar Glad ,  Bo-Lennart Johansson ,  Jean-Luc Maloisel ,  Nils Norrman

    发明人： Gunnar Glad ,  Bo-Lennart Johansson ,  Jean-Luc Maloisel ,  Nils Norrman

    IPC分类号： G01N33/53 ,  C08G63/91 ,  C07K16/18

    CPC分类号： B01J20/3212 ,  B01D15/426 ,  B01J20/286 ,  B01J20/289 ,  B01J20/3219 ,  B01J20/3251

    摘要： The present invention relates to a separation matrix comprised of a porous support to which ligands have been immobilised, wherein said ligands comprise at least one sulphonamide and the R group of the sulphonyl comprises an aromatic group. The nitrogen of the sulphonamide may be a secondary or tertiary amine. The invention also relates to a chromatography column that contains the described separation matrix, as well as to a method of isolating immunoglobulin-like compounds by adsorption to the separation matrix.

    摘要翻译： 本发明涉及由已经固定有配体的多孔载体组成的分离基质，其中所述配体包含至少一个磺酰胺，并且磺酰基的R基包括芳族基团。 磺酰胺的氮可以是仲胺或叔胺。 本发明还涉及含有所述分离基质的色谱柱，以及通过吸附到分离基质上分离免疫球蛋白样化合物的方法。

    Method for production of separation media
    5.
    发明授权
    Method for production of separation media 失效
    分离介质的生产方法

    公开(公告)号：US08372286B2

    公开(公告)日：2013-02-12

    申请号：US12863448

    申请日：2009-01-30

    申请人： Gunnar Glad ,  Bo-Lennart Johansson ,  Jean-Luc Maloisel ,  Nils Norrman ,  Ake Stenholm

    发明人： Gunnar Glad ,  Bo-Lennart Johansson ,  Jean-Luc Maloisel ,  Nils Norrman ,  Ake Stenholm

    IPC分类号： B01D15/08

    CPC分类号： B01J20/291 ,  B01J20/262 ,  B01J20/2809 ,  B01J20/285 ,  B01J20/286 ,  B01J20/287 ,  B01J20/288 ,  B01J20/3212 ,  B01J20/3268 ,  B01J20/3274 ,  B01J20/3293 ,  B01J39/26 ,  B01J41/20 ,  B01J2220/52

    摘要： The present invention relates to a method for production of separation media using a so called Spinning Disc technology wherein the porosities of the beads are optimized in such a way that a desired biomolecule may be separated from a complex sample. The method comprises the following steps: a) feeding a 4-8% polysaccharide solution, which has a viscosity within 350-450 mPas, at 65-75° C. to one or more spinning discs at 3001-3010 rpm to form polysaccharide beads; b) capturing said formed polysaccharide beads in a capturing bath; wherein the porosity of the polysaccharide beads is controlled by varying the temperature of the capturing between 15 and 27° C., preferably between 17.5 and 24.6° C. The method yields porosities that prevent molecules larger than 150 000 g/mol to diffuse into the beads. The invention also relates to separation media produced by the method and use thereof for purification of biomolecules, in particular monoclonal antibodies.

    摘要翻译： 本发明涉及使用所谓的旋转圆盘技术生产分离介质的方法，其中珠子的孔隙度以使得所需生物分子可以与复杂样品分离的方式进行优化。 该方法包括以下步骤：a）将具有350-450mPa·s粘度的4-8％多糖溶液在65-75℃下以3001-3010rpm向一个或多个纺丝盘送入，以形成多糖珠粒 ; b）在捕获浴中捕获所述形成的多糖珠粒; 其中多糖珠的孔隙率通过在15和27℃之间，优选在17.5和24.6℃之间改变捕获温度来控制。该方法产生阻止大于150000g / mol的分子扩散到 珠子 本发明还涉及通过其用于纯化生物分子，特别是单克隆抗体的方法和用途产生的分离培养基。

    Separation matrix and method of purification
    6.
    发明授权
    Separation matrix and method of purification 有权
    分离基质和纯化方法

    公开(公告)号：US08114280B2

    公开(公告)日：2012-02-14

    申请号：US11570530

    申请日：2005-06-22

    申请人： Jean-Luc Maloisel ,  Bo-Lennart Johansson ,  Gunnar Glad ,  Nils Norrman

    发明人： Jean-Luc Maloisel ,  Bo-Lennart Johansson ,  Gunnar Glad ,  Nils Norrman

    IPC分类号： B01D15/08

    CPC分类号： B01J20/3212 ,  B01D15/426 ,  B01J20/286 ,  B01J20/289 ,  B01J20/3219 ,  B01J20/3251

    摘要： The present invention relates to a separation matrix comprised of a porous support to which ligands have been immobilized, wherein said ligands comprise at least one sulphonamide and the R group of the sulphonyl comprises an aromatic group. The nitrogen of the sulphonamide may be a secondary or tertiary amine. The invention also relates to a chromatography column that contains the described separation matrix, as well as to a method of isolating immunoglobulin-like compounds by adsorption to the separation matrix.

    摘要翻译： 本发明涉及由已经固定有配体的多孔载体组成的分离基质，其中所述配体包含至少一个磺酰胺，并且磺酰基的R基包括芳族基团。 磺酰胺的氮可以是仲胺或叔胺。 本发明还涉及含有所述分离基质的色谱柱，以及通过吸附到分离基质上分离免疫球蛋白样化合物的方法。

    METHOD FOR PRODUCTION OF SEPARATION MEDIA
    7.
    发明申请
    METHOD FOR PRODUCTION OF SEPARATION MEDIA 失效
    分离介质的生产方法

    公开(公告)号：US20100298548A1

    公开(公告)日：2010-11-25

    申请号：US12863448

    申请日：2009-01-30

    申请人： Gunnar Glad ,  Bo-Lennart Johansson ,  Jean-Luc Maloisel ,  Nils Norrman ,  Ake Stenholm

    发明人： Gunnar Glad ,  Bo-Lennart Johansson ,  Jean-Luc Maloisel ,  Nils Norrman ,  Ake Stenholm

    IPC分类号： C07K16/00 ,  B29B9/10 ,  B01D15/04 ,  B29B9/12

    CPC分类号： B01J20/291 ,  B01J20/262 ,  B01J20/2809 ,  B01J20/285 ,  B01J20/286 ,  B01J20/287 ,  B01J20/288 ,  B01J20/3212 ,  B01J20/3268 ,  B01J20/3274 ,  B01J20/3293 ,  B01J39/26 ,  B01J41/20 ,  B01J2220/52

    摘要： The present invention relates to a method for production of separation media using a so called Spinning Disc technology wherein the porosities of the beads are optimized in such a way that a desired biomolecule may be separated from a complex sample. The method comprises the following steps: a) feeding a 4-8% polysaccharide solution, which has a viscosity within 350-450 mPas, at 65-75° C. to one or more spinning discs at 3001-3010 rpm to form polysaccharide beads; b) capturing said formed polysaccharide beads in a capturing bath; wherein the porosity of the polysaccharide beads is controlled by varying the temperature of the capturing between 15 and 27° C., preferably between 17.5 and 24.6° C. The method yields porosities that prevent molecules larger than 150 000 g/mol to diffuse into the beads. The invention also relates to separation media produced by the method and use thereof for purification of biomolecules, in particular monoclonal antibodies.

    摘要翻译： 本发明涉及使用所谓的旋转圆盘技术生产分离介质的方法，其中珠子的孔隙度以使得所需生物分子可以与复杂样品分离的方式进行优化。 该方法包括以下步骤：a）将具有350-450mPa·s粘度的4-8％多糖溶液在65-75℃下以3001-3010rpm向一个或多个纺丝盘送入，以形成多糖珠 ; b）在捕获浴中捕获所述形成的多糖珠粒; 其中多糖珠的孔隙率通过在15和27℃之间，优选在17.5和24.6℃之间改变捕获温度来控制。该方法产生阻止大于150000g / mol的分子扩散到 珠子 本发明还涉及通过其用于纯化生物分子，特别是单克隆抗体的方法和用途产生的分离培养基。

    Hydrophobic Interaction Chromatography
    8.
    发明申请
    Hydrophobic Interaction Chromatography 审中-公开
    疏水相互作用色谱

    公开(公告)号：US20080299671A1

    公开(公告)日：2008-12-04

    申请号：US12095752

    申请日：2006-11-30

    申请人： Gunnar Glad ,  Bo-Lennart Johansson ,  Jean-Luc Maloisel ,  Nils Norrman

    发明人： Gunnar Glad ,  Bo-Lennart Johansson ,  Jean-Luc Maloisel ,  Nils Norrman

    IPC分类号： G01N30/02 ,  B01D15/16 ,  B01D15/32 ,  C07K16/06 ,  C07K1/00

    CPC分类号： C07K16/065 ,  B01D15/327 ,  B01J20/262 ,  B01J20/265 ,  B01J20/285 ,  B01J20/286 ,  B01J20/289

    摘要： The present invention relates to a method of isolating target compounds from a liquid, which method including the steps of providing a mobile phase, which contains at least one target compound and wherein the conductivity corresponds to ≧0.6 M; contacting the mobile phase with a separation matrix including one or more sulphonamide groups to adsorb one or more target compounds; contacting an eluent with the matrix to release target compound(s), wherein the conductivity of the eluent is reduced as compared to the mobile phase conductivity and the pH is substantially equivalent to the mobile phase pH; and, optionally, recovering at least one target compound or a purified liquid.

    摘要翻译： 本发明涉及一种从液体中分离目标化合物的方法，该方法包括以下步骤：提供含有至少一种目标化合物的流动相，其中电导率对应于> = 0.6M; 使流动相与包含一个或多个磺酰胺基团的分离基质接触以吸附一种或多种目标化合物; 使洗脱液与基质接触以释放目标化合物，其中与流动相电导率相比，洗脱液的电导率降低，并且pH基本上等于流动相pH; 和任选地回收至少一种目标化合物或纯化液体。