-
公开(公告)号：US20110045574A1
公开(公告)日：2011-02-24
申请号：US12988707
申请日：2009-04-21
申请人： Jan Bergstrom , Gunnar Glad , Bo-Lennart Johansson , Jean-Luc Maloisel , Nils Norrman , Tobias E. Soderman
发明人： Jan Bergstrom , Gunnar Glad , Bo-Lennart Johansson , Jean-Luc Maloisel , Nils Norrman , Tobias E. Soderman
IPC分类号： C12N7/02 , H01F1/00 , C12N1/20 , C12N15/63 , B01J20/00 , B01J20/26 , C07K16/00 , C07K1/14 , C07H21/00 , C11B3/00
CPC分类号： B01D15/327 , B01J20/28009 , B01J20/285 , B01J20/287 , B01J20/3212 , B01J20/3293 , B01J39/26 , C07K1/20 , C07K16/00 , C12N7/00 , C12N2760/16151 , G01N1/405
摘要： The present invention is within the field of chromatography. More precisely, it relates to a novel chromatography medium, namely a hydrophobic medium provided with different lids excluding molecules over a certain size due to the porosity of the hydrophobic medium and/or the porosity of the lid. The invention also relates to use of the separation medium for purification of large molecules, which do not enter the separation medium, as well as small molecules, which enter the separation medium and are eluted from there.
摘要翻译： 本发明在色谱领域内。 更准确地说，它涉及一种新颖的色谱介质，即由于疏水介质的孔隙率和/或盖的孔隙度，提供了不同盖子的疏水介质，其中排除了一定尺寸的分子。 本发明还涉及用于纯化不分离介质的大分子的分离介质以及进入分离介质并从其中洗脱的小分子的用途。
-
公开(公告)号：US20110155668A1
公开(公告)日：2011-06-30
申请号：US13002878
申请日：2009-06-04
CPC分类号： B01D15/3828 , B01D15/166 , B01D15/36 , B01J20/28092 , B01J20/285 , B01J20/286 , B01J20/3242 , B01J20/3293 , C07K1/16 , C07K1/22 , C07K16/00
摘要： The present invention relates to a separation medium, comprising an inner core of a porous material provided with charged ligands, and an outer lid comprising a porous material provided with charged ligands, wherein the charge of the ligands in the inner core is opposite that of the charge of the ligands in the lid. The present invention also relates to a method for biomolecule separation comprising applying a sample to the above separation medium, wherein large molecules are prevented from entering the medium by charge repulsion from the medium and small molecules are captured in the inner core.
摘要翻译： 本发明涉及一种分离介质，其包括具有带电配位体的多孔材料的内核和包含带有带电配位体的多孔材料的外盖，其中内芯中配体的电荷与 盖中配体的电荷。 本发明还涉及一种用于生物分子分离的方法，包括将样品施加到上述分离介质中，其中通过从介质的电荷排斥来防止大分子进入介质，并且小分子被捕获在内核中。
-
公开(公告)号：US20070196858A1
公开(公告)日：2007-08-23
申请号：US11570530
申请日：2005-06-22
CPC分类号： B01J20/3212 , B01D15/426 , B01J20/286 , B01J20/289 , B01J20/3219 , B01J20/3251
摘要： The present invention relates to a separation matrix comprised of a porous support to which ligands have been immobilised, wherein said ligands comprise at least one sulphonamide and the R group of the sulphonyl comprises an aromatic group. The nitrogen of the sulphonamide may be a secondary or tertiary amine. The invention also relates to a chromatography column that contains the described separation matrix, as well as to a method of isolating immunoglobulin-like compounds by adsorption to the separation matrix.
摘要翻译： 本发明涉及由已经固定有配体的多孔载体组成的分离基质，其中所述配体包含至少一个磺酰胺，并且磺酰基的R基包括芳族基团。 磺酰胺的氮可以是仲胺或叔胺。 本发明还涉及含有所述分离基质的色谱柱，以及通过吸附到分离基质上分离免疫球蛋白样化合物的方法。
-
公开(公告)号：US20080299671A1
公开(公告)日：2008-12-04
申请号：US12095752
申请日：2006-11-30
CPC分类号： C07K16/065 , B01D15/327 , B01J20/262 , B01J20/265 , B01J20/285 , B01J20/286 , B01J20/289
摘要： The present invention relates to a method of isolating target compounds from a liquid, which method including the steps of providing a mobile phase, which contains at least one target compound and wherein the conductivity corresponds to ≧0.6 M; contacting the mobile phase with a separation matrix including one or more sulphonamide groups to adsorb one or more target compounds; contacting an eluent with the matrix to release target compound(s), wherein the conductivity of the eluent is reduced as compared to the mobile phase conductivity and the pH is substantially equivalent to the mobile phase pH; and, optionally, recovering at least one target compound or a purified liquid.
摘要翻译： 本发明涉及一种从液体中分离目标化合物的方法，该方法包括以下步骤：提供含有至少一种目标化合物的流动相，其中电导率对应于> = 0.6M; 使流动相与包含一个或多个磺酰胺基团的分离基质接触以吸附一种或多种目标化合物; 使洗脱液与基质接触以释放目标化合物，其中与流动相电导率相比，洗脱液的电导率降低，并且pH基本上等于流动相pH; 和任选地回收至少一种目标化合物或纯化液体。
-
公开(公告)号：US08685248B2
公开(公告)日：2014-04-01
申请号：US12709556
申请日：2010-02-22
IPC分类号： B01D15/08
CPC分类号： B01J20/262 , B01J20/267 , B01J20/28019 , B01J20/28023 , B01J20/28033 , B01J20/28042 , B01J20/286 , B01J20/289 , B01J20/3092 , B01J20/3208 , B01J20/3212 , B01J20/3219 , B01J20/3246 , B01J20/3248 , B01J20/3251 , B01J20/3255 , B01J20/3272 , B01J20/3285 , B01J2220/54 , B01J2220/58 , C07K16/065 , Y10T428/249963
摘要： The present invention relates to a separation matrix comprised of a porous support to which ligands have been immobilized, wherein said ligands comprise at least one aliphatic sulphonamide. The nitrogen of the sulphonamide may be a secondary or tertiary amine. The invention also relates to a chromatography column that contains the described separation matrix, as well as to a method of isolating immunoglobulin-like compounds by adsorption to a separation matrix that comprises aliphatic sulphonamide ligands.
摘要翻译： 本发明涉及由已经固定有配体的多孔载体组成的分离基质，其中所述配体包含至少一种脂族磺酰胺。 磺酰胺的氮可以是仲胺或叔胺。 本发明还涉及含有所述分离基质的色谱柱，以及通过吸附到包含脂族磺酰胺配体的分离基质上分离免疫球蛋白样化合物的方法。
-
公开(公告)号：US08372286B2
公开(公告)日：2013-02-12
申请号：US12863448
申请日：2009-01-30
IPC分类号： B01D15/08
CPC分类号： B01J20/291 , B01J20/262 , B01J20/2809 , B01J20/285 , B01J20/286 , B01J20/287 , B01J20/288 , B01J20/3212 , B01J20/3268 , B01J20/3274 , B01J20/3293 , B01J39/26 , B01J41/20 , B01J2220/52
摘要： The present invention relates to a method for production of separation media using a so called Spinning Disc technology wherein the porosities of the beads are optimized in such a way that a desired biomolecule may be separated from a complex sample. The method comprises the following steps: a) feeding a 4-8% polysaccharide solution, which has a viscosity within 350-450 mPas, at 65-75° C. to one or more spinning discs at 3001-3010 rpm to form polysaccharide beads; b) capturing said formed polysaccharide beads in a capturing bath; wherein the porosity of the polysaccharide beads is controlled by varying the temperature of the capturing between 15 and 27° C., preferably between 17.5 and 24.6° C. The method yields porosities that prevent molecules larger than 150 000 g/mol to diffuse into the beads. The invention also relates to separation media produced by the method and use thereof for purification of biomolecules, in particular monoclonal antibodies.
摘要翻译： 本发明涉及使用所谓的旋转圆盘技术生产分离介质的方法，其中珠子的孔隙度以使得所需生物分子可以与复杂样品分离的方式进行优化。 该方法包括以下步骤：a）将具有350-450mPa·s粘度的4-8％多糖溶液在65-75℃下以3001-3010rpm向一个或多个纺丝盘送入，以形成多糖珠粒 ; b）在捕获浴中捕获所述形成的多糖珠粒; 其中多糖珠的孔隙率通过在15和27℃之间，优选在17.5和24.6℃之间改变捕获温度来控制。该方法产生阻止大于150000g / mol的分子扩散到 珠子 本发明还涉及通过其用于纯化生物分子，特别是单克隆抗体的方法和用途产生的分离培养基。
-
公开(公告)号：US08114280B2
公开(公告)日：2012-02-14
申请号：US11570530
申请日：2005-06-22
IPC分类号： B01D15/08
CPC分类号： B01J20/3212 , B01D15/426 , B01J20/286 , B01J20/289 , B01J20/3219 , B01J20/3251
摘要： The present invention relates to a separation matrix comprised of a porous support to which ligands have been immobilized, wherein said ligands comprise at least one sulphonamide and the R group of the sulphonyl comprises an aromatic group. The nitrogen of the sulphonamide may be a secondary or tertiary amine. The invention also relates to a chromatography column that contains the described separation matrix, as well as to a method of isolating immunoglobulin-like compounds by adsorption to the separation matrix.
摘要翻译： 本发明涉及由已经固定有配体的多孔载体组成的分离基质，其中所述配体包含至少一个磺酰胺，并且磺酰基的R基包括芳族基团。 磺酰胺的氮可以是仲胺或叔胺。 本发明还涉及含有所述分离基质的色谱柱，以及通过吸附到分离基质上分离免疫球蛋白样化合物的方法。
-
公开(公告)号：US20100298548A1
公开(公告)日：2010-11-25
申请号：US12863448
申请日：2009-01-30
CPC分类号： B01J20/291 , B01J20/262 , B01J20/2809 , B01J20/285 , B01J20/286 , B01J20/287 , B01J20/288 , B01J20/3212 , B01J20/3268 , B01J20/3274 , B01J20/3293 , B01J39/26 , B01J41/20 , B01J2220/52
摘要： The present invention relates to a method for production of separation media using a so called Spinning Disc technology wherein the porosities of the beads are optimized in such a way that a desired biomolecule may be separated from a complex sample. The method comprises the following steps: a) feeding a 4-8% polysaccharide solution, which has a viscosity within 350-450 mPas, at 65-75° C. to one or more spinning discs at 3001-3010 rpm to form polysaccharide beads; b) capturing said formed polysaccharide beads in a capturing bath; wherein the porosity of the polysaccharide beads is controlled by varying the temperature of the capturing between 15 and 27° C., preferably between 17.5 and 24.6° C. The method yields porosities that prevent molecules larger than 150 000 g/mol to diffuse into the beads. The invention also relates to separation media produced by the method and use thereof for purification of biomolecules, in particular monoclonal antibodies.
摘要翻译： 本发明涉及使用所谓的旋转圆盘技术生产分离介质的方法，其中珠子的孔隙度以使得所需生物分子可以与复杂样品分离的方式进行优化。 该方法包括以下步骤：a）将具有350-450mPa·s粘度的4-8％多糖溶液在65-75℃下以3001-3010rpm向一个或多个纺丝盘送入，以形成多糖珠 ; b）在捕获浴中捕获所述形成的多糖珠粒; 其中多糖珠的孔隙率通过在15和27℃之间，优选在17.5和24.6℃之间改变捕获温度来控制。该方法产生阻止大于150000g / mol的分子扩散到 珠子 本发明还涉及通过其用于纯化生物分子，特别是单克隆抗体的方法和用途产生的分离培养基。
-
公开(公告)号：US20110118442A1
公开(公告)日：2011-05-19
申请号：US12984132
申请日：2011-01-04
申请人： CARINA ENGSTRAND , Annika Forss , Gunnar Glad , Bo-Lennart Johansson , Hans J. Johansson , Jean-Luc Maloisel
发明人： CARINA ENGSTRAND , Annika Forss , Gunnar Glad , Bo-Lennart Johansson , Hans J. Johansson , Jean-Luc Maloisel
IPC分类号： C07K1/16 , C07C211/00 , A61L2/00 , B01D15/08
CPC分类号： B01D15/3828 , B01D15/327 , B01D15/363 , B01D15/3804 , B01D15/3809 , B01D15/3847 , B01J20/286 , B01J20/3253 , B01J20/3285 , B01J41/20 , C07K1/165 , C07K1/36 , C07K16/00 , C07K16/065
摘要： The present invention relates to a chromatography ligand defined by the following formula R1—R2—N(R3)—R4—R5 wherein R1 is a substituted or non-substituted phenyl group; R2 is a hydrocarbon chain comprising 0-4 carbon atoms; R3 is a hydrocarbon chain comprising 1-3 carbon atoms; R4 is a hydrocarbon chain comprising 1-5 carbon atoms; and R5 is OH or H. The invention also comprises a separation matrix, comprising the described ligands coupled to a porous support, such as particles or a membrane. The ligand and matrix according to the invention is useful for purification of biomolecules or organic compounds, such as proteins, polypeptides, DNA etc. An advantageous use according to the invention is the purification of antibodies.
-
公开(公告)号：US07714112B2
公开(公告)日：2010-05-11
申请号：US11577203
申请日：2005-10-21
申请人： Carina Engstrand , Annika Forss , Gunnar Glad , Bo-Lennart Johansson , Hans J. Johansson , Jean-Luc Maloisel
发明人： Carina Engstrand , Annika Forss , Gunnar Glad , Bo-Lennart Johansson , Hans J. Johansson , Jean-Luc Maloisel
CPC分类号： B01D15/3828 , B01D15/327 , B01D15/363 , B01D15/3804 , B01D15/3809 , B01D15/3847 , B01J20/286 , B01J20/3253 , B01J20/3285 , B01J41/20 , C07K1/165 , C07K1/36 , C07K16/00 , C07K16/065
摘要： The present invention relates to a method of separating antibodies from other compound(s) in a liquid sample, wherein a mobile phase comprising said sample is contacted with a multi-modal separation matrix to adsorb undesired compounds while the antibodies remain free in the liquid, wherein the multi-modal separation matrix comprises first groups, which are capable of interacting with negatively charged sites of the target compounds, and second groups, which are capable of at least one interaction other than charge-charge interaction with said target compounds. The invention also relates to a chromatography column packed with the above-described multi-modal separation matrix and a filter having such multi-modal groups adsorbed to its surface.
摘要翻译： 本发明涉及从液体样品中的其它化合物分离抗体的方法，其中包含所述样品的流动相与多模式分离基质接触以吸附不期望的化合物，同时抗体在液体中保持游离， 其中所述多模式分离基质包含能够与所述目标化合物的带负电荷位点相互作用的第一组和能够与所述目标化合物的电荷 - 电荷相互作用以外的至少一个相互作用的第二组。 本发明还涉及一种填充有上述多模式分离基质的色谱柱，以及具有吸附在其表面上的多模式基团的过滤器。
-
-
-
-
-
-
-
-
-
搜索结果
33 条记录 (耗时 0.028 秒)
