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    Pentacyclic compounds and their use as absorption or fluorescent dyes
    12.
    发明授权
    Pentacyclic compounds and their use as absorption or fluorescent dyes 失效
    五环化合物及其作为吸收或荧光染料的用途

    公开(公告)号：US5750409A

    公开(公告)日：1998-05-12

    申请号：US454440

    申请日：1995-05-30

    申请人： Rupert Herrmann ,  Hans-Peter Josel ,  Karl-Heinz Drexhage ,  Jutta Arden-Jacob

    发明人： Rupert Herrmann ,  Hans-Peter Josel ,  Karl-Heinz Drexhage ,  Jutta Arden-Jacob

    IPC分类号： C09B11/24 ,  C09B11/28 ,  G01N33/58 ,  G01N33/557 ,  C07D221/18 ,  C07D491/00 ,  G01N33/536

    CPC分类号： G01N33/582 ,  C09B11/24 ,  C09B11/28 ,  Y10S436/80

    摘要： Pentacyclic derivatives having the general formulae (Ia), (Ib) and (Ic) ##STR1## denote: hydrogen, alkyl with 1 to 20 carbon atoms polyoxyhydrocarbyl, phenyl, phenylalkyl with 1 to 3 carbon atoms in the alkyl chain, wherein the alkyl residues or/and phenyl residues can be substituted by one or several hydroxy, halogen, sulfo, carboxy or alkoxycarbonyl groups in which alkoxy can have 1 to 4 carbon atoms; R.sup.7 denotes an alkyl group with 1 to 7 carbon atoms, substituted by at least one halogen, or denotes a carboxyalkyl group or a phenyl group which is substituted by a carboxy or alkoxycarbonyl group located at the o-position relative to the carbon atom bound to the pentacyclic ring system and by at least one halogen, wherein alkoxy can have 1 to 4 carbon atoms, or a carboxymethylene-oxy-alkyloxy group; the residues R.sup.14 and R.sup.15 as well as the positions on the rings in the formulae that are not marked by specific symbols denote any desired substituents which may optionally be linked together; the two bonds marked by a broken line mean that the two carbon atoms which are joined by the bond marked by a broken line can be linked together by a single or double bond; and X.sup.- is a counterion; as well as their derivatives activated at least at one of the residues R.sup.1, R.sup.7 or/and R.sup.13. The compounds can be used as absorption or fluorescent dyes, in particular as markers for hapten/antibody/protein conjugates, as laser dyes and as an absorption medium for solar collectors.

    摘要翻译： （Ⅰa）（Ⅰa）（Ⅰa）表示氢，碳原子数1〜20的烷基，聚氧烃基，苯基， 在烷基链中具有1至3个碳原子的苯基烷基，其中烷基残基或/和苯基残基可以被一个或多个羟基，卤素，磺基，羧基或烷氧基羰基取代，其中烷氧基可以具有1至4个碳原子; R7表示具有1至7个碳原子的烷基，被至少一个卤素取代，或表示羧基烷基或被位于相对于与碳原子结合的碳原子的o位置的羧基或烷氧基羰基取代的苯基 五环环系和至少一个卤素，其中烷氧基可以具有1至4个碳原子，或羧基亚甲氧基 - 烷氧基; 残基R14和R15以及未被特定符号标记的式中的环上的位置表示可任选地连接在一起的任何所需取代基; 由虚线标记的两个键意味着通过用虚线标记的键连接的两个碳原子可以通过单键或双键连接在一起; X-是抗衡离子; 以及至少在残基R1，R7或/和R13之一激活的它们的衍生物。 该化合物可用作吸收或荧光染料，特别是作为半抗原/抗体/蛋白质缀合物的标记物，作为激光染料和作为太阳能收集器的吸收介质。

    Use of a bis-maleic anhydride cross-linking agent for fixation of a cell or tissue sample
    14.
    发明授权
    Use of a bis-maleic anhydride cross-linking agent for fixation of a cell or tissue sample 失效
    双马来酸酐交联剂用于固定细胞或组织样品的用途

    公开(公告)号：US08460944B2

    公开(公告)日：2013-06-11

    申请号：US13400862

    申请日：2012-02-21

    申请人： Hans-Peter Josel ,  Michael Gerg ,  Rupert Herrmann ,  Rosa Isabel Saez Diaz

    发明人： Hans-Peter Josel ,  Michael Gerg ,  Rupert Herrmann ,  Rosa Isabel Saez Diaz

    IPC分类号： G01N33/557 ,  G01N33/543 ,  G01N33/556 ,  C12Q1/68 ,  C07D407/00 ,  B01L3/00

    CPC分类号： G01N1/30

    摘要： The present disclosure relates to novel bis-maleic anhydrides and to the surprising discovery that bis-maleic anhydride cross-linking agents can be used for preservation/fixation of a cell or tissue sample. Various bis-maleic anhydride cross-linking agent scan be used in methods requiring fixation of a cell or tissue sample. These reagents and methods are especially useful in procedures that require that the fixation agent be removed in order to facilitate analysis with other reagents. The inventive reagents and methods make it easier to reliably assay for various proteins, a nucleic acid and the like using analytical methods such as like immunohistochemistry, fluorescence in situ hybridization, RT-PCR, and the like.

    摘要翻译： 本公开涉及新颖的双马来酸酐，以及令人惊奇的发现，即双马来酸酐交联剂可用于保存/固定细胞或组织样品。 各种双马来酸酐交联剂扫描用于需要固定细胞或组织样品的方法。 这些试剂和方法在需要除去固定剂以便于与其它试剂分析的方法中特别有用。 本发明的试剂和方法使得可以使用诸如免疫组织化学，荧光原位杂交，RT-PCR等的分析方法更容易地可靠地测定各种蛋白质，核酸等。

    MEASUREMENT OF VITAMIN D
    15.
    发明申请
    MEASUREMENT OF VITAMIN D 有权
    维生素D的测定

    公开(公告)号：US20100285603A1

    公开(公告)日：2010-11-11

    申请号：US12326972

    申请日：2008-12-03

    申请人： Uwe Kobold ,  Thomas Duelffer ,  Michael Grol ,  Rupert Herrmann ,  Herbert Von der Eltz ,  Leopold Von Proff

    发明人： Uwe Kobold ,  Thomas Duelffer ,  Michael Grol ,  Rupert Herrmann ,  Herbert Von der Eltz ,  Leopold Von Proff

    IPC分类号： G01N33/00

    CPC分类号： G01N33/82 ,  Y10T436/20 ,  Y10T436/203332

    摘要： The present invention relates to a method of measuring a vitamin D metabolite in a sample, the method comprising the steps of (a) treating said sample with a vitamin D metabolite releasing reagent under conditions appropriate to release a vitamin D metabolite from vitamin D-binding protein and not to cause protein precipitation, (b) subjecting the treated sample obtained in step (a) to a chromatographic separation, and (c) measuring a vitamin D metabolite during or after said chromatographic separation. The present invention also relates to methods for determining the vitamin D status of a subject, for use in the diagnosis of disease, and to agents and kits for use in performing the methods of the invention.

    摘要翻译： 本发明涉及一种测量样品中维生素D代谢物的方法，所述方法包括以下步骤：（a）在适于从维生素D结合物释放维生素D代谢物的条件下用维生素D代谢物释放试剂处理所述样品 蛋白质，不会引起蛋白质沉淀，（b）使步骤（a）中得到的处理样品进行色谱分离，和（c）在所述色谱分离期间或之后测量维生素D代谢物。 本发明还涉及确定用于疾病诊断的受试者的维生素D状态的方法以及用于实施本发明的方法的试剂和试剂盒。

    READY-TO-USE WHOLE BLOOD COLLECTION VESSEL
    16.
    发明申请
    READY-TO-USE WHOLE BLOOD COLLECTION VESSEL 有权
    准备使用完整的血液收集血管

    公开(公告)号：US20090246866A1

    公开(公告)日：2009-10-01

    申请号：US12327854

    申请日：2008-12-04

    申请人： Werner Naser ,  Thomas Duelffer ,  Rupert Herrmann ,  Uwe Kobold ,  Herbert von der Eltz

    发明人： Werner Naser ,  Thomas Duelffer ,  Rupert Herrmann ,  Uwe Kobold ,  Herbert von der Eltz

    IPC分类号： C12M3/08

    CPC分类号： G01N33/5002

    摘要： The present invention relates to a sampling tube for collecting and processing a whole blood sample. The sampling tube contains a reagent for differential hemolysis of whole blood, wherein said reagent for differential hemolysis comprises a chemical for differential hemolysis and an anti-coagulant, and wherein said sampling tube is a ready-to-use and single-use sampling tube. It also relates to the use of said sampling tube in the processing of a whole blood sample for liquid chromatography and also to the use of a blood sample processed in such sampling tube in a liquid chromatography-based analysis.

    摘要翻译： 本发明涉及一种用于收集和处理全血样本的取样管。 采样管含有用于全血差异溶血的试剂，其中所述用于差异溶血的试剂包括用于差异溶血的化学药剂和抗凝血剂，并且其中所述采样管是即用型和一次性采样管。 它还涉及在液相色谱法的全血样品的处理中使用所述采样管以及在基于液相色谱的分析中使用在这种采样管中处理的血液样品。

    Process for the detection of substances with hydrolase activity
    18.
    发明授权
    Process for the detection of substances with hydrolase activity 失效
    检测具有水解酶活性的物质的方法

    公开(公告)号：US5202233A

    公开(公告)日：1993-04-13

    申请号：US816795

    申请日：1992-01-03

    申请人： Rupert Herrmann ,  Hans-Joachim Guder ,  Werner Guthlein ,  Manfred Kuhr ,  Johann Berger ,  Harvey Buck

    发明人： Rupert Herrmann ,  Hans-Joachim Guder ,  Werner Guthlein ,  Manfred Kuhr ,  Johann Berger ,  Harvey Buck

    IPC分类号： C12Q1/34 ,  G01N33/58

    CPC分类号： C12Q1/34 ,  G01N33/581 ,  C12Q2334/30 ,  G01N2333/924 ,  Y10S435/805

    摘要： A process for the detection of substances with hydrolase activity in a sample by mixing the sample with a hydrolase substrate and an oxidizing agent, and the evaluating the resultant color intensity, wherein, as hydrolase substrate, there is used at least one compound of the formula ##STR1## in which R.sup.1 is hydrogen or an alkoxy radical,R.sup.2 is hydrogen or halogen, an amino group or an alkoxy or aralkoxy radical,R.sup.3, R.sup.4, R.sup.5 and R.sup.6, which can be the same or different, are hydrogen, halogen, carboxyl, a carbamoyl group, sulpho, an amino groups alkyl, an alkoxy radical, aralkoxy, alkylcarbonyl or alkoxycarbonyl, andX is a glycosyl, phosphate or acyl residue.Optionally R.sup.2 and R.sup.3, R.sup.3 and R.sup.4, R.sup.4 and R.sup.5, and R.sup.5 and R.sup.6 can be joined to form a ring system. At least one of R.sup.1 and R.sup.2 must be hydrogen and at least one of R.sup.1, R.sup.2 and R.sup.3 must not be hydrogen. Compounds in which X is .beta.-galactosyl are new.

    摘要翻译： 一种用于通过将样品与水解酶底物和氧化剂混合来检测样品中水解酶活性的物质并评估所得颜色强度的方法，其中作为水解酶底物，使用至少一种式 其中R 1是氢或烷氧基，R 2是氢或卤素，氨基或烷氧基或芳烷氧基，可以相同或不同的R 3，R 4，R 5和R 6是氢，卤素， 羧基，氨基甲酰基，磺基，氨基烷基，烷氧基，芳烷氧基，烷基羰基或烷氧基羰基，X是糖基，磷酸酯或酰基残基。 任选地，R 2和R 3，R 3和R 4，R 4和R 5以及R 5和R 6可以连接形成环系。 R 1和R 2中的至少一个必须是氢，R 1，R 2和R 3中的至少一个不能是氢。 X是β-半乳糖基的化合物是新的。

    Preparation of samples for LC-MS/MS using magnetic particles
    20.
    发明授权
    Preparation of samples for LC-MS/MS using magnetic particles 有权
    使用磁性颗粒制备LC-MS / MS样品

    公开(公告)号：US07815803B2

    公开(公告)日：2010-10-19

    申请号：US12133545

    申请日：2008-06-05

    申请人： Uwe Kobold ,  Albert Geiger ,  Rupert Herrmann ,  Michael Vogeser

    发明人： Uwe Kobold ,  Albert Geiger ,  Rupert Herrmann ,  Michael Vogeser

    IPC分类号： B01D15/08 ,  B01D59/44 ,  H01J49/00

    CPC分类号： G01N33/6848 ,  B01D15/325 ,  B01J20/28009 ,  B01J20/287 ,  B01J20/3246 ,  B01J20/3276 ,  G01N33/54326 ,  G01N33/6842

    摘要： The present invention provides a novel procedure of preparing samples for analysis by way of mass spectrometry, preferably LC-MS/MS. Accordingly, functionalized magnetic particles with a hydrophobic surface were used for extracting low molecular weight compounds from complex liquid biological samples such as plasma, serum, whole blood or hemolyzed blood. The method of the invention includes (a) contacting the sample with an amount of functionalized magnetic particles with a hydrophobic surface, (b) incubating the sample and the particles, thereby adsorbing the compound to the hydrophobic surface, (c) separating the particles by applying a magnetic field and removing the liquid, (d) optionally washing the particles, (e) eluting the compound from the particles.

    摘要翻译： 本发明提供了通过质谱法，优选LC-MS / MS制备用于分析的样品的新方法。 因此，使用具有疏水性表面的官能化磁性颗粒从复杂的液体生物样品如血浆，血清，全血或溶血中提取低分子量化合物。 本发明的方法包括（a）使样品与一定量的具有疏水性表面的官能化磁性颗粒接触，（b）将样品和颗粒温育，从而将化合物吸附到疏水表面，（c）将颗粒分离 施加磁场并除去液体，（d）任选地洗涤颗粒，（e）从颗粒中洗脱化合物。