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公开(公告)号:US20180179575A1
公开(公告)日:2018-06-28
申请号:US15848640
申请日:2017-12-20
发明人: Wayne N. George , Alexandre Richez , M. Shane Bowen , Andrew A. Brown , Dajun M. Yuan , Audrey Rose Zak , Sean M. Ramirez , Raymond Campos
IPC分类号: C12Q1/68 , C09D183/06 , C09D133/26
摘要: An example of an array includes a support, a cross-linked epoxy polyhedral oligomeric silsesquioxane (POSS) resin film on a surface of the support, and a patterned hydrophobic polymer layer on the cross-linked epoxy POSS resin film. The patterned hydrophobic polymer layer defines exposed discrete areas of the cross-linked epoxy POSS resin film, and a polymer coating is attached to the exposed discrete areas. Another example of an array includes a support, a modified epoxy POSS resin film on a surface of the support, and a patterned hydrophobic polymer layer on the modified epoxy POSS resin film. The modified epoxy POSS resin film includes a polymer growth initiation site, and the patterned hydrophobic polymer layer defines exposed discrete areas of the modified epoxy POSS resin film. A polymer brush is attached to the polymer growth initiation site in the exposed discrete areas.
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公开(公告)号:US20180155469A1
公开(公告)日:2018-06-07
申请号:US15809656
申请日:2017-11-10
发明人: Andrew A. Brown , Wayne N. George , Alexandre Richez , Anne-Cecile Dingwall , Xavier von Hatten
IPC分类号: C08F20/60 , C08F293/00 , C08F8/32 , C08F220/56 , C12Q1/6874 , C40B50/18
CPC分类号: C08F20/60 , B01J2219/00637 , B01J2219/00722 , C08F8/32 , C08F220/56 , C08F293/005 , C08F2438/03 , C12Q1/6874 , C40B50/18 , C08F220/60 , C08F2220/603 , C08F222/385 , C08F220/22 , C08F2220/346
摘要: Some embodiments described herein relate to new polymer coatings for surface functionalization and new processes for grafting pre-grafted DNA-copolymers to surface(s) of substrates for use in DNA sequencing and other diagnostic applications.
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公开(公告)号:US09982250B2
公开(公告)日:2018-05-29
申请号:US14831062
申请日:2015-08-20
发明人: Xavier von Hatten , Wayne N. George , Alexandre Richez , Anne-Cecile Dingwall , Andrew A. Brown
IPC分类号: C12Q1/68 , C12N11/10 , G01N33/543 , G01N33/551
CPC分类号: C12N11/10 , C12Q1/6834 , C12Q1/6869 , G01N33/543 , G01N33/54353 , G01N33/551 , C12Q2563/131 , C12Q2565/543
摘要: Some embodiments described herein relate to a substrate comprising a silane functionalized surface for reversibly immobilizing a biological molecule of interest, such as oligonucleotides, polynucleotides, or protein. Methods for immobilizing the biological molecule and the use in DNA sequencing and other diagnostic applications are also disclosed.
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公开(公告)号:US20160053252A1
公开(公告)日:2016-02-25
申请号:US14831062
申请日:2015-08-20
发明人: Xavier von Hatten , Wayne N. George , Alexandre Richez , Anne-Cecile Dingwall , Andrew A. Brown
IPC分类号: C12N11/10
CPC分类号: C12N11/10 , C12Q1/6834 , C12Q1/6869 , G01N33/543 , G01N33/54353 , G01N33/551 , C12Q2563/131 , C12Q2565/543
摘要: Some embodiments described herein relate to a substrate comprising a silane functionalized surface for reversibly immobilizing a biological molecule of interest, such as oligonucleotides, polynucleotides, or protein. Methods for immobilizing the biological molecule and the use in DNA sequencing and other diagnostic applications are also disclosed.
摘要翻译: 本文所述的一些实施方案涉及包含硅烷官能化表面的底物,用于可逆地固定目标生物分子,例如寡核苷酸,多核苷酸或蛋白质。 还公开了用于固定生物分子的方法以及在DNA测序和其他诊断应用中的用途。
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公开(公告)号:US20230100285A1
公开(公告)日:2023-03-30
申请号:US17934406
申请日:2022-09-22
IPC分类号: C08L83/06
摘要: An example of an ultraviolet light curable resin composition includes a predetermined mass ratio of a first epoxy substituted polyhedral oligomeric silsesquioxane monomer and a second substituted polyhedral oligomeric silsesquioxane monomer, wherein the first and second epoxy substituted polyhedral oligomeric silsesquioxane monomers are different, and wherein the predetermined mass ratio ranges from about 3:7 to about 7:3; bis-(4-methylphenyl)iodonium hexafluorophosphate as a first initiator; a second initiator selected from the group consisting of a free radical initiator and a cationic initiator other than bis-(4-methylphenyl)iodonium hexafluorophosphate; a surface additive; and a solvent.
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公开(公告)号:US20220185927A1
公开(公告)日:2022-06-16
申请号:US17600072
申请日:2020-12-01
发明人: Gianluca Andrea Artioli , Andrew A. Brown , Wayne N. George , Colin Pilkington , Jem Pitcairn , Alexandre Richez , Xavier von Hatten
IPC分类号: C08F220/60 , C08F2/38
摘要: A hydrogel includes a dendritic core with 2 to 30 arms, and first and second acrylamide monomers incorporated into each arm. The first acrylamide monomer is: (I), wherein R1 and R2 are independently selected from an alkyl, an alkylamino, an alkylamido, an alkylthio, an aryl, a glycol, and optionally substituted variants thereof; and the second acrylamide monomer is: (II), wherein R3 and R4 are independently hydrogen or an alkyl; L is a linker including a linear chain of 2 to 20 atoms selected from carbon, oxygen, and nitrogen and optional substituents on the carbon and any nitrogen atoms; A is an N substituted amide: (III), where R5 is hydrogen or an alkyl; E is a linear chain of 1 to 4 atom(s) selected from carbon, oxygen and nitrogen, and optional substituents on the carbon and any nitrogen atoms; and Z is an optional nitrogen containing heterocycle.
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公开(公告)号:US20180327832A1
公开(公告)日:2018-11-15
申请号:US15969562
申请日:2018-05-02
发明人: Sean M. Ramirez , Brian D. Mather , Edwin Li , Sojeong Moon , Innsu Daniel Kim , Alexandre Richez , Ludovic Vincent , Xavier von Hatten , Hai Quang Tran , Maxwell Zimmerley , Julia Morrison , Gianluca Andrea Artioli , Krystal Sly , Hayden Black , Lewis J. Kraft , Hong Xie , Wei Wei , Ryan Sanford
IPC分类号: C12Q1/6874 , C12Q1/6806
CPC分类号: C12Q1/6874 , C12Q1/6806
摘要: An example of a method includes providing a substrate with an exposed surface comprising a first chemical group, wherein the providing optionally comprises modifying the exposed surface of the substrate to incorporate the first chemical group; reacting the first chemical group with a first reactive group of a functionalized polymer molecule to form a functionalized polymer coating layer covalently bound to the exposed surface of the substrate; grafting a primer to the functionalized polymer coating layer by reacting the primer with a second reactive group of the functionalized polymer coating layer; and forming a water-soluble protective coating on the primer and the functionalized polymer coating layer. Examples of flow cells incorporating examples of the water-soluble protective coating are also disclosed herein.
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公开(公告)号:US11512339B2
公开(公告)日:2022-11-29
申请号:US15848640
申请日:2017-12-20
发明人: Wayne N. George , Alexandre Richez , M. Shane Bowen , Andrew A. Brown , Dajun Yuan , Audrey Rose Zak , Sean M. Ramirez , Raymond Campos
IPC分类号: C09D183/06 , C12Q1/68 , C09D133/26 , C08G77/14
摘要: An example of an array includes a support, a cross-linked epoxy polyhedral oligomeric silsesquioxane (POSS) resin film on a surface of the support, and a patterned hydrophobic polymer layer on the cross-linked epoxy POSS resin film. The patterned hydrophobic polymer layer defines exposed discrete areas of the cross-linked epoxy POSS resin film, and a polymer coating is attached to the exposed discrete areas. Another example of an array includes a support, a modified epoxy POSS resin film on a surface of the support, and a patterned hydrophobic polymer layer on the modified epoxy POSS resin film. The modified epoxy POSS resin film includes a polymer growth initiation site, and the patterned hydrophobic polymer layer defines exposed discrete areas of the modified epoxy POSS resin film. A polymer brush is attached to the polymer growth initiation site in the exposed discrete areas.
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公开(公告)号:US11447582B2
公开(公告)日:2022-09-20
申请号:US16739679
申请日:2020-01-10
发明人: Andrew A. Brown , Wayne N. George , Alexandre Richez , Anne-Cecile Dingwall , Xavier von Hatten
IPC分类号: C08F8/32 , C12Q1/6874 , C08F220/56 , C08F20/60 , C08F293/00 , C08F285/00 , C40B50/18 , C08F220/28 , C08F220/34
摘要: Some embodiments described herein relate to new polymer coatings for surface functionalization and new processes for grafting pre-grafted DNA-copolymers to surface(s) of substrates for use in DNA sequencing and other diagnostic applications.
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公开(公告)号:US11213976B2
公开(公告)日:2022-01-04
申请号:US15847150
申请日:2017-12-19
发明人: Alexandre Richez , Andrew A. Brown , Julia Morrison , Wayne N. George , Timothy J. Merkel , Audrey Rose Zak
摘要: An imprinting apparatus includes a silicon master having a plurality of nanofeatures defined therein. An anti-stick layer coats the silicon master, the anti-stick layer including a molecule having a cyclosiloxane with at least one silane functional group. A method includes forming a master template by: depositing a formulation on a silicon master including a plurality of nanofeatures defined therein, the formulation including a solvent and a molecule having a cyclosiloxane with at least one silane functional group; and curing the formulation, thereby forming an anti-stick layer on the silicon master, the anti-stick layer including the molecule. The method further includes depositing a silicon-based working stamp material on the anti-stick layer of the master template; curing the silicon-based working stamp material to form a working stamp including a negative replica of the plurality of nanofeatures; and releasing the working stamp from the master template.
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