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    Method and apparatus for amplifying nucleic acids
    11.
    发明申请
    Method and apparatus for amplifying nucleic acids 有权
    扩增核酸的方法和装置

    公开(公告)号：US20050202489A1

    公开(公告)日：2005-09-15

    申请号：US11071383

    申请日：2005-03-03

    申请人： Yoon-kyoung Cho ,  Joon-ho Kim ,  Kak Namkoong ,  Geun-bae Lim ,  Jun-hong Min

    发明人： Yoon-kyoung Cho ,  Joon-ho Kim ,  Kak Namkoong ,  Geun-bae Lim ,  Jun-hong Min

    IPC分类号： C12N15/09 ,  B01F5/06 ,  B01F13/00 ,  B01F15/02 ,  B01L3/00 ,  B01L7/00 ,  C12M1/00 ,  C12M1/38 ,  C12N15/00 ,  C12Q1/68 ,  G01N35/00 ,  G01N35/08 ,  C12P19/34

    CPC分类号： G01N35/08 ,  B01F5/0646 ,  B01F5/0647 ,  B01F5/0654 ,  B01F13/0071 ,  B01F2015/0221 ,  B01J2219/00783 ,  B01J2219/00824 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00837 ,  B01J2219/0086 ,  B01J2219/00873 ,  B01J2219/00891 ,  B01L3/5027 ,  B01L3/502715 ,  B01L2300/0809 ,  B01L2300/0861 ,  B01L2300/0883 ,  G01N2035/00366 ,  G01N2035/00514

    摘要： Provided are a method and apparatus for amplifying nucleic acids. The method includes introducing into a reaction vessel via different inlet channels a reactant aqueous solution containing reactants for nucleic acid amplification and a fluid that is phase-separated from the reactant aqueous solution and does not participate in amplification reaction, creating a plurality of reactant aqueous solution droplets surrounded by the fluid by contacting the reactant aqueous solution with the fluid in the reaction vessel, and amplifying the nucleic acids in the reactant aqueous solution droplets. The apparatus includes a substrate, a reaction vessel formed inside of the substrate, at least one first inlet channel formed inside the substrate, connected to an end of the reaction vessel, and allowing introduction of a reactant aqueous solution containing reactants for nucleic acid amplification into the reaction vessel, a second inlet channel formed inside the substrate, connected to the end of the reaction vessel, and allowing introduction of a fluid that is phase-separated from the reactant aqueous solution and does not participate in amplification reaction into the reaction vessel, and a heating unit installed on the substrate in such a way to thermally contact with the substrate and heating the substrate.

    摘要翻译： 提供了用于扩增核酸的方法和装置。 该方法包括通过不同的入口通道将含有用于核酸扩增的反应物的反应物水溶液和与反应物水溶液相分离并且不参与扩增反应的流体引入反应容器，形成多个反应物水溶液 通过使反应物水溶液与反应容器中的流体接触，并且扩增反应物水溶液液滴中的核酸，由流体包围的液滴。 该装置包括基板，形成在基板内部的反应容器，形成在基板内部的至少一个第一入口通道，与反应容器的端部连接，并且允许将含有用于核酸扩增的反应物的反应物水溶液引入到 反应容器，形成在基板内部的第二入口通道，连接到反应容器的端部，并且允许引入与反应物水溶液相分离并且不参与扩增反应的流体进入反应容器， 以及加热单元，其以与基板热接触并加热基板的方式安装在基板上。

    Sensing switch and detecting method using the same
    12.
    发明授权
    Sensing switch and detecting method using the same 有权
    感应开关及使用其的检测方法

    公开(公告)号：US07790440B2

    公开(公告)日：2010-09-07

    申请号：US12272183

    申请日：2008-11-17

    申请人： Kyu-tae Yoo ,  Joon-ho Kim ,  Jun-hong Min ,  Sung-ouk Jung ,  Ji-na Namgoong ,  Kui-hyun Kim ,  Jeo-young Shim ,  Kak Namkoong

    发明人： Kyu-tae Yoo ,  Joon-ho Kim ,  Jun-hong Min ,  Sung-ouk Jung ,  Ji-na Namgoong ,  Kui-hyun Kim ,  Jeo-young Shim ,  Kak Namkoong

    IPC分类号： C12M3/00

    CPC分类号： G01N33/54373

    摘要： Provided are a sensing switch and a sensing method using the same. The sensing switch includes: a substrate; a supporter on the substrate; a sensing plate that is connected to a side of the supporter and is in parallel with the substrate by a predetermined distance; a receptor binding region on an upper surface of an end portion of the sensing plate; an electric or magnetic field generation device that induces deflection of the sensing plate when a receptor bound to the receptor binding region is selectively bound to an electrically or magnetically active ligand; and a pair of switching electrodes that are separated by a predetermined distance and is connected when the sensing plate contacts the substrate due to the deflection of the sensing plate. A target material need not be labelled, a signal processing of a fluorescent or electrical detection signal using an analysis apparatus is not required, and a signal can be directly decoded by confirming whether a current flows through the switch.

    摘要翻译： 提供了一种感测开关和使用该感测开关的感测方法。 感测开关包括：基板; 底物上的支撑物; 感测板，其与所述支撑体的一侧连接并且与所述基板平行预定距离; 在感测板的端部的上表面上的受体结合区域; 当与受体结合区结合的受体选择性地结合到电或磁性活性配体时，引起感测板偏转的电场或磁场产生装置; 以及一对分离预定距离的开关电极，并且当感测板由于感测板的偏转而与基板接触时被连接。 目标材料不需要标注，不需要使用分析装置的荧光或电检测信号的信号处理，并且可以通过确认电流是否流过开关直接解码信号。

    Method of isolating and purifying nucleic acids using immobilized hydrogel or PEG-hydrogel copolymer
    14.
    发明申请
    Method of isolating and purifying nucleic acids using immobilized hydrogel or PEG-hydrogel copolymer 失效
    使用固定化水凝胶或PEG-水凝胶共聚物分离和纯化核酸的方法

    公开(公告)号：US20060134675A1

    公开(公告)日：2006-06-22

    申请号：US11297783

    申请日：2005-12-07

    申请人： Chang-eun Yoo ,  Joon-ho Kim ,  Kyu-youn Hwang ,  Hun-joo Lee ,  Hee-kyun Lim ,  Jun-hong Min

    发明人： Chang-eun Yoo ,  Joon-ho Kim ,  Kyu-youn Hwang ,  Hun-joo Lee ,  Hee-kyun Lim ,  Jun-hong Min

    IPC分类号： C12Q1/68 ,  C07H21/04

    CPC分类号： C07H21/04

    摘要： Provided is a method of isolating and purifying nucleic acids using an immobilized hydrogel or polyethylene glycol (PEG)-hydrogel copolymer. The method includes: immobilizing a functional group-containing hydrogel or PEG-hydrogel copolymer on a substrate; adding a mixed sample solution containing a salt and nucleic acids to the hydrogel- or PEG-hydrogel copolymer-immobilized substrate to bind the nucleic acids to the hydrogel or the PEG-hydrogel copolymer; washing the nucleic acid-bound hydrogel or PEG-hydrogel copolymer; and eluting the nucleic acids from the hydrogel or the PEG-hydrogel copolymer using an elution solvent. Therefore, binding and elution of nucleic acids can be performed even with no addition of a separate chemical substance, and an effect on a subsequent process such as PCR can be minimized. Furthermore, the amount and intensity for binding nucleic acids can be adjusted according to PEG concentration, and the presence of a hydrogel compound on a substrate enables patterning.

    摘要翻译： 提供了使用固定的水凝胶或聚乙二醇（PEG） - 水凝胶共聚物来分离和纯化核酸的方法。 该方法包括：将含官能团的水凝胶或PEG-水凝胶共聚物固定在基材上; 将含有盐和核酸的混合样品溶液加入到水凝胶或PEG-水凝胶共聚物固定的底物中，以将核酸与水凝胶或PEG-水凝胶共聚物结合; 洗涤核酸结合的水凝胶或PEG-水凝胶共聚物; 并使用洗脱溶剂从水凝胶或PEG-水凝胶共聚物中洗脱核酸。 因此，即使没有添加单独的化学物质也可以进行核酸的结合和洗脱，并且可以最小化对后续过程（例如PCR）的影响。 此外，可以根据PEG浓度调节结合核酸的量和强度，并且在基材上存在水凝胶化合物能够进行图案化。

    Nucleic acid purification method using hydrogen bonding and electric field
    15.
    发明申请
    Nucleic acid purification method using hydrogen bonding and electric field 审中-公开
    使用氢键和电场的核酸纯化方法

    公开(公告)号：US20060118417A1

    公开(公告)日：2006-06-08

    申请号：US11281226

    申请日：2005-11-16

    申请人： Young-a Kim ,  Jun-hong Min ,  Kui-hyun Kim ,  Myo-yong Lee ,  Su-hyeon Kim ,  Young-nam Kwon ,  Jeong-gun Lee ,  Joon-ho Kim

    发明人： Young-a Kim ,  Jun-hong Min ,  Kui-hyun Kim ,  Myo-yong Lee ,  Su-hyeon Kim ,  Young-nam Kwon ,  Jeong-gun Lee ,  Joon-ho Kim

    IPC分类号： C07H21/04

    CPC分类号： C07H21/04

    摘要： Provided is a method of purifying nucleic acids using hydrogen bonding and an electric field, including: bringing a sample containing target nucleic acids into contact with an electrode coated with a material capable of forming hydrogen bonds with the target nucleic acids; applying a positive voltage to the electrode to move the target nucleic acids closer to the electrode so as to form hydrogen bonds with the material on the electrode; washing the electrode; and applying to the electrode a negative voltage to elute the bound target nucleic acids. According to the method, selectivity to nucleic acids and proteins increases due to hydrogen bonding, nucleic acid purification is possible within a short time through an electric field, and the bound nucleic acids can be efficiently eluted.

    摘要翻译： 提供了使用氢键和电场来净化核酸的方法，包括：使含有靶核酸的样品与涂覆有能够与靶核酸形成氢键的材料接触; 向电极施加正电压以使靶核酸更靠近电极以与电极上的材料形成氢键; 清洗电极; 并向电极施加负电压以洗脱结合的靶核酸。 根据该方法，由于氢键，核酸和蛋白质的选择性增加，核酸纯化可以在短时间内通过电场进行，并且结合的核酸可以被有效地洗脱。

    Method and apparatus for amplifying nucleic acids
    16.
    发明授权
    Method and apparatus for amplifying nucleic acids 有权
    扩增核酸的方法和装置

    公开(公告)号：US07943348B2

    公开(公告)日：2011-05-17

    申请号：US12504271

    申请日：2009-07-16

    申请人： Yoon-kyoung Cho ,  Joon-ho Kim ,  Kak Namkoong ,  Geun-bae Lim ,  Jun-hong Min

    发明人： Yoon-kyoung Cho ,  Joon-ho Kim ,  Kak Namkoong ,  Geun-bae Lim ,  Jun-hong Min

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： G01N35/08 ,  B01F5/0646 ,  B01F5/0647 ,  B01F5/0654 ,  B01F13/0071 ,  B01F2015/0221 ,  B01J2219/00783 ,  B01J2219/00824 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00837 ,  B01J2219/0086 ,  B01J2219/00873 ,  B01J2219/00891 ,  B01L3/5027 ,  B01L3/502715 ,  B01L2300/0809 ,  B01L2300/0861 ,  B01L2300/0883 ,  G01N2035/00366 ,  G01N2035/00514

    摘要： A method and apparatus for amplifying nucleic acids. The method includes introducing into a reaction vessel via different inlet channels a reactant aqueous solution containing reactants for nucleic acid amplification and a fluid that is phase-separated from the reactant aqueous solution and does not participate in amplification reaction, creating a plurality of reactant aqueous solution droplets surrounded by the fluid by contacting the reactant aqueous solution with the fluid in the reaction vessel, and amplifying the nucleic acids in the reactant aqueous solution droplets. The apparatus includes a substrate, a reaction vessel formed inside of the substrate, at least one first inlet channel formed inside the substrate, connected to an end of the reaction vessel, and allowing introduction of a reactant aqueous solution containing reactants for nucleic acid amplification into the reaction vessel, a second inlet channel formed inside the substrate, connected to the end of the reaction vessel, and allowing introduction of a fluid that is phase-separated from the reactant aqueous solution and does not participate in amplification reaction into the reaction vessel, and a heating unit installed on the substrate in such a way to thermally contact with the substrate and heating the substrate.

    摘要翻译： 用于扩增核酸的方法和装置。 该方法包括通过不同的入口通道将含有用于核酸扩增的反应物的反应物水溶液和与反应物水溶液相分离并且不参与扩增反应的流体引入反应容器，形成多个反应物水溶液 通过使反应物水溶液与反应容器中的流体接触，并且扩增反应物水溶液液滴中的核酸，由流体包围的液滴。 该装置包括基板，形成在基板内部的反应容器，形成在基板内部的至少一个第一入口通道，与反应容器的端部连接，并且允许将含有用于核酸扩增的反应物的反应物水溶液引入到 反应容器，形成在基板内部的第二入口通道，连接到反应容器的端部，并且允许引入与反应物水溶液相分离并且不参与扩增反应的流体进入反应容器， 以及加热单元，其以与基板热接触并加热基板的方式安装在基板上。

    METHOD AND APPARATUS FOR AMPLIFYING NUCLEIC ACIDS
    18.
    发明申请
    METHOD AND APPARATUS FOR AMPLIFYING NUCLEIC ACIDS 有权
    用于放大核酸的方法和装置

    公开(公告)号：US20100021972A1

    公开(公告)日：2010-01-28

    申请号：US12504271

    申请日：2009-07-16

    申请人： Yoon-kyoung Cho ,  Joon-ho Kim ,  Kak Namkoong ,  Geun-bae Lim ,  Jun-hong Min

    发明人： Yoon-kyoung Cho ,  Joon-ho Kim ,  Kak Namkoong ,  Geun-bae Lim ,  Jun-hong Min

    IPC分类号： C12P19/34

    CPC分类号： G01N35/08 ,  B01F5/0646 ,  B01F5/0647 ,  B01F5/0654 ,  B01F13/0071 ,  B01F2015/0221 ,  B01J2219/00783 ,  B01J2219/00824 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00837 ,  B01J2219/0086 ,  B01J2219/00873 ,  B01J2219/00891 ,  B01L3/5027 ,  B01L3/502715 ,  B01L2300/0809 ,  B01L2300/0861 ,  B01L2300/0883 ,  G01N2035/00366 ,  G01N2035/00514

    摘要： A method and apparatus for amplifying nucleic acids. The method includes introducing into a reaction vessel via different inlet channels a reactant aqueous solution containing reactants for nucleic acid amplification and a fluid that is phase-separated from the reactant aqueous solution and does not participate in amplification reaction, creating a plurality of reactant aqueous solution droplets surrounded by the fluid by contacting the reactant aqueous solution with the fluid in the reaction vessel, and amplifying the nucleic acids in the reactant aqueous solution droplets. The apparatus includes a substrate, a reaction vessel formed inside of the substrate, at least one first inlet channel formed inside the substrate, connected to an end of the reaction vessel, and allowing introduction of a reactant aqueous solution containing reactants for nucleic acid amplification into the reaction vessel, a second inlet channel formed inside the substrate, connected to the end of the reaction vessel, and allowing introduction of a fluid that is phase-separated from the reactant aqueous solution and does not participate in amplification reaction into the reaction vessel, and a heating unit installed on the substrate in such a way to thermally contact with the substrate and heating the substrate.

    摘要翻译： 用于扩增核酸的方法和装置。 该方法包括通过不同的入口通道将含有用于核酸扩增的反应物的反应物水溶液和与反应物水溶液相分离并且不参与扩增反应的流体引入反应容器，形成多个反应物水溶液 通过使反应物水溶液与反应容器中的流体接触，并且扩增反应物水溶液液滴中的核酸，由流体包围的液滴。 该装置包括基板，形成在基板内部的反应容器，形成在基板内部的至少一个第一入口通道，与反应容器的端部连接，并且允许将含有用于核酸扩增的反应物的反应物水溶液引入到 反应容器，形成在基板内部的第二入口通道，连接到反应容器的端部，并且允许引入与反应物水溶液相分离并且不参与扩增反应的流体进入反应容器， 以及加热单元，其以与基板热接触并加热基板的方式安装在基板上。