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公开(公告)号:US20050006239A1
公开(公告)日:2005-01-13
申请号:US10843526
申请日:2004-05-10
申请人: Joseph Amshey , Timothy Updyke , Thomas Jackson , Michael Thacker
发明人: Joseph Amshey , Timothy Updyke , Thomas Jackson , Michael Thacker
IPC分类号: B01D61/58 , C07K1/26 , G01L1/20 , G01N27/447
CPC分类号: G01N27/44795 , G01N27/44704
摘要: A device for fluid phase electrophoresis, particularly solution phase isoelectric focusing, components thereof, and methods for use are presented.
摘要翻译: 提出了一种用于流相相电泳的装置,特别是溶液相等电聚焦,其组分和使用方法。
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公开(公告)号:US20050103628A1
公开(公告)日:2005-05-19
申请号:US10948453
申请日:2004-09-22
申请人: Thomas Jackson , Adam Henry , Joseph Amshey , Roumen Bogoev
发明人: Thomas Jackson , Adam Henry , Joseph Amshey , Roumen Bogoev
IPC分类号: G01N27/447 , G01N27/453
CPC分类号: G01N27/44704 , G01N27/44782
摘要: Apparatus and methods for conducting electrophoretic separation concurrently in a plurality of gels with improved reproducibility among the gels.
摘要翻译: 在多个凝胶中同时进行电泳分离的装置和方法,其具有改进的凝胶重现性。
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公开(公告)号:US08845984B2
公开(公告)日:2014-09-30
申请号:US13615500
申请日:2012-09-13
申请人: Joseph Amshey , Daniel Bezdek , Espir Kahatt , Alexander Khorlin , Lance Parsons , Todd Peterson , Timothy Powers , Michael Thacker , Timothy Updyke , Kornelija Zgonc
发明人: Joseph Amshey , Daniel Bezdek , Espir Kahatt , Alexander Khorlin , Lance Parsons , Todd Peterson , Timothy Powers , Michael Thacker , Timothy Updyke , Kornelija Zgonc
CPC分类号: B01L3/502715 , B01L3/50273 , B01L7/00 , B01L9/527 , B01L2200/025 , B01L2200/027 , B01L2200/0689 , B01L2200/10 , B01L2200/16 , B01L2300/0636 , B01L2300/0681 , B01L2300/0867 , B01L2300/088 , B01L2300/123 , B01L2400/0481 , B01L2400/0487 , B01L2400/0605 , B01L2400/0655 , G01N2035/00306 , G01N2035/1037 , Y10T436/2575
摘要: The present invention provides systems, devices, apparatuses and methods for automated bioprocessing. Examples of protocols and bioprocessing procedures suitable for the present invention include but are not limited to: immunoprecipitation, chromatin immunoprecipitation, recombinant protein isolation, nucleic acid separation and isolation, protein labeling, separation and isolation, cell separation and isolation, food safety analysis and automatic bead based separation. In some embodiments, the invention provides automated systems, automated devices, automated cartridges and automated methods of western blot processing. Other embodiments include automated systems, automated devices, automated cartridges and automated methods for separation, preparation and purification of nucleic acids, such as DNA or RNA or fragments thereof, including plasmid DNA, genomic DNA, bacterial DNA, viral DNA and any other DNA, and for automated systems, automated devices, automated cartridges and automated methods for processing, separation and purification of proteins, peptides and the like.
摘要翻译: 本发明提供了用于自动化生物处理的系统,装置,装置和方法。 适用于本发明的方案和生物处理方法的实例包括但不限于:免疫沉淀,染色质免疫沉淀,重组蛋白分离,核酸分离和分离,蛋白标记,分离和分离,细胞分离和分离,食品安全分析和自动 珠基分离。 在一些实施方案中,本发明提供自动化系统,自动化装置,自动化盒和western印迹处理的自动化方法。 其他实施方案包括自动化系统,自动化装置,自动化盒和用于分离,制备和纯化核酸例如DNA或RNA或其片段的自动化方法,包括质粒DNA,基因组DNA,细菌DNA,病毒DNA和任何其它DNA, 以及用于自动化系统,自动化设备,自动化盒和用于处理,分离和纯化蛋白质,肽等的自动化盒和自动化方法。
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公开(公告)号:US20100120129A1
公开(公告)日:2010-05-13
申请号:US12549311
申请日:2009-08-27
申请人: Joseph Amshey , Daniel Bezdek , Espir Kahatt , Alexander Khorlin , Lance Parsons , Todd Peterson , Timothy Powers , Michael Thacker , Timothy Updyke , Kornelija Zgonc
发明人: Joseph Amshey , Daniel Bezdek , Espir Kahatt , Alexander Khorlin , Lance Parsons , Todd Peterson , Timothy Powers , Michael Thacker , Timothy Updyke , Kornelija Zgonc
CPC分类号: B01L3/502715 , B01L3/50273 , B01L7/00 , B01L9/527 , B01L2200/025 , B01L2200/027 , B01L2200/0689 , B01L2200/10 , B01L2200/16 , B01L2300/0636 , B01L2300/0681 , B01L2300/0867 , B01L2300/088 , B01L2300/123 , B01L2400/0481 , B01L2400/0487 , B01L2400/0605 , B01L2400/0655 , G01N2035/00306 , G01N2035/1037 , Y10T436/2575
摘要: The present invention provides systems, devices, apparatuses and methods for automated bioprocessing. Examples of protocols and bioprocessing procedures suitable for the present invention include but are not limited to: immunoprecipitation, chromatin immunoprecipitation, recombinant protein isolation, nucleic acid separation and isolation, protein labeling, separation and isolation, cell separation and isolation, food safety analysis and automatic bead based separation. In some embodiments, the invention provides automated systems, automated devices, automated cartridges and automated methods of western blot processing. Other embodiments include automated systems, automated devices, automated cartridges and automated methods for separation, preparation and purification of nucleic acids, such as DNA or RNA or fragments thereof, including plasmid DNA, genomic DNA, bacterial DNA, viral DNA and any other DNA, and for automated systems, automated devices, automated cartridges and automated methods for processing, separation and purification of proteins, peptides and the like.
摘要翻译: 本发明提供了用于自动化生物处理的系统,装置,装置和方法。 适用于本发明的方案和生物处理方法的实例包括但不限于:免疫沉淀,染色质免疫沉淀,重组蛋白分离,核酸分离和分离,蛋白标记,分离和分离,细胞分离和分离,食品安全分析和自动 珠基分离。 在一些实施方案中,本发明提供自动化系统,自动化装置,自动化盒和western印迹处理的自动化方法。 其他实施方案包括自动化系统,自动化装置,自动化盒和用于分离,制备和纯化核酸例如DNA或RNA或其片段的自动化方法,包括质粒DNA,基因组DNA,细菌DNA,病毒DNA和任何其它DNA, 以及用于自动化系统,自动化设备,自动化盒和用于处理,分离和纯化蛋白质,肽等的自动化盒和自动化方法。
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公开(公告)号:US20050106605A1
公开(公告)日:2005-05-19
申请号:US10949471
申请日:2004-09-24
申请人: Joseph Amshey , Roumen Bogoev
发明人: Joseph Amshey , Roumen Bogoev
CPC分类号: G01N33/6842 , C40B40/10 , G01N2496/00
摘要: The invention provides populations of molecules that are prepared as, or treated to become, homogeneous for one or more molecular characteristics. In an aspect, the invention relates to molecular weight standards that may be used to determine the molecular weight or apparent molecular weight of uncharacterized molecules, such as proteins and nucleic acids, as well as in other applications. In one aspect, the molecular weight standards are pre-stained.
摘要翻译: 本发明提供了为一种或多种分子特征制备或被处理成均匀的分子群体。 在一方面,本发明涉及可用于测定未表征的分子(例如蛋白质和核酸)以及其它应用的分子量或表观分子量的分子量标准品。 在一个方面,分子量标准物被预染色。
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公开(公告)号:US20050034989A1
公开(公告)日:2005-02-17
申请号:US10861787
申请日:2004-06-04
申请人: Roumen Bogoev , Scott Whitney , Joseph Amshey
发明人: Roumen Bogoev , Scott Whitney , Joseph Amshey
IPC分类号: G01N33/48 , C07K1/34 , C12M1/00 , C12M1/33 , C12N15/09 , C12Q1/68 , G01N1/10 , G01N1/28 , G01L1/20
CPC分类号: C07K1/34 , Y10T436/25375 , Y10T436/255 , Y10T436/2575
摘要: An apparatus and method for mincing a gel includes a gel mincing tube and a mesh material. The mesh material extends across the end of the tube. To subdivide a gel using the mincing apparatus, a gel is placed upon the mesh material in the mincing tube, the mincing tube, mesh material and the gel are spun in a centrifuge, forcing the gel through the mesh material so that the gel is subdivided into generally uniform smaller fragments. The mesh material may be secured to a tube in the form of a nesting tube. The nesting tube nests within the opening of a recovery vessel. The mesh material may be placed in series with a conditionally porous membrane in the nesting tube. Centrifuging the nesting tube and the recovery vessel subdivides gel material into fragments by forcing the gel through the mesh material. The gel subsequently falls upon the membrane, and may be treated on the membrane to extract or otherwise treat analytes in the gel material.
摘要翻译: 用于切割凝胶的设备和方法包括凝胶切割管和网状材料。 网状材料延伸穿过管的端部。 为了使用切碎装置对凝胶进行细分,将凝胶放置在打孔管中的网状材料上,在离心机中将切细管,网状材料和凝胶纺丝,迫使凝胶通过网状材料,使凝胶细分 变成大致均匀的较小碎片。 网状材料可以以嵌套管的形式固定到管。 嵌套管嵌套在回收容器的开口内。 网状材料可以与嵌套管中的条状多孔膜串联放置。 将嵌套管和回收容器离心通过使凝胶通过网状材料将凝胶材料细分成碎片。 凝胶随后落在膜上,并且可以在膜上处理以提取或以其它方式处理凝胶材料中的分析物。
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公开(公告)号:US20050023141A1
公开(公告)日:2005-02-03
申请号:US10895427
申请日:2003-05-09
申请人: Joseph Amshey , Timothy Updyke , Thomas Jackson , Michael Thacker
发明人: Joseph Amshey , Timothy Updyke , Thomas Jackson , Michael Thacker
IPC分类号: B01D61/42 , C25B9/00 , G01N27/447 , G01R1/00
CPC分类号: B01D61/42 , G01N27/44795
摘要: A device for fluid phase electrophoresis, particularly solution phase isoelectric focusing, components thereof, and methods for use are presented.
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