-
公开(公告)号：US10294519B2
公开(公告)日：2019-05-21
申请号：US14233256
申请日：2012-05-16
申请人： Toshiro Saito , Koshin Hamasaki , Satoshi Takahashi , Muneo Maeshima , Kyoko Imai , Kazumichi Imai , Ryuji Tao
发明人： Toshiro Saito , Koshin Hamasaki , Satoshi Takahashi , Muneo Maeshima , Kyoko Imai , Kazumichi Imai , Ryuji Tao
IPC分类号： C12Q1/6834 , C12N15/10
摘要： A convenient method for nucleic acid analysis is provided, which enables 1000 or more types of nucleic acid to be analyzed collectively with high comprehensiveness and with a dynamic range of at least four digits. In particular, provided is a very effective analytical method especially for untranslated RNAs and microRNAs, of which the types of target nucleic acids is 10000 or lower. Nucleic acids can be analyzed conveniently and rapidly with high comprehensiveness and quantitative performance at single-molecule sensitivity and resolution by following the steps of: preparing a group of target nucleic acid fragments one molecule at a time and hybridizing the nucleic acid molecules, which have known base sequences and have been labeled with the fluorescence substances, with the group of the target nucleic acid fragments to detect the fluorescence substances labeling the hybridized nucleic acid molecules.
-
公开(公告)号：US09109194B2
公开(公告)日：2015-08-18
申请号：US13505803
申请日：2010-11-05
申请人： Toshifumi Honda , Hiroko Fujita , Muneo Maeshima , Akira Maekawa , Yoshiko Ishida , Yuta Urano , Shinya Murakami
发明人： Toshifumi Honda , Hiroko Fujita , Muneo Maeshima , Akira Maekawa , Yoshiko Ishida , Yuta Urano , Shinya Murakami
摘要： When multiple kinds of bacterial colonies are present in a petri dish and, for example, a drug tolerance is to be measured, harvesting of mixed colonies of different types of bacteria makes it impossible to accurately determine the drug tolerance. Also, it is required to improve the throughput of a device for harvesting a bacterial colony. From images illuminated from multiple directions, isolating bacterial colonies are automatically extracted. Next, the image feature amounts are calculated from the multiple images that are illuminated from multiple directions and colonies are grouped depending on the feature amounts. Then, bacterial colonies to be harvested are determined based on the results of the grouping.
摘要翻译： 当培养皿中存在多种细菌菌落时，例如测定药物耐受性时，收集不同类型细菌的混合菌落使得不可能准确地确定药物耐受性。 此外，需要提高用于收获细菌菌落的装置的产量。 从多个方向照射的图像，分离细菌菌落被自动提取。 接下来，根据从多个方向照明的多个图像计算图像特征量，并且根据特征量对殖民地进行分组。 然后，基于分组的结果确定要收获的细菌菌落。
-
公开(公告)号：US20120141330A1
公开(公告)日：2012-06-07
申请号：US13382316
申请日：2010-07-05
IPC分类号： G01N21/00
CPC分类号： G01N21/51 , G01N15/0205 , G01N21/253 , G01N2015/0065 , G01N2035/00396 , G01N2035/0453
摘要： To be adapted to various types of latex reagents for detecting scattered light and thereby measuring agglutination reactions with high sensitivity while sufficiently ensuring integration time. To be adapted to various types of latex particles of different particle sizes, a plurality of light receivers are arranged in a plane perpendicular to the direction of cell movement by rotation of a cell disk. To ensure sufficient integration time, the angle between the optical axis of the irradiation light and each of a plurality of optical axes of scattered light viewed from above the cell is made equal to or less than 17.7° including a mounting error.
摘要翻译： 适用于各种类型的用于检测散射光的乳胶试剂，从而以高灵敏度测量凝集反应，同时充分确保积分时间。 为了适应于各种类型的不同粒度的胶乳颗粒，多个光接收器通过旋转细胞盘而布置在垂直于细胞移动方向的平面中。 为了确保足够的积分时间，使得照射光的光轴与从电池单元的上方观察的散射光的多个光轴中的每一个之间的角度等于或小于包括安装误差的17.7°。
-
公开(公告)号：US20050196778A1
公开(公告)日：2005-09-08
申请号：US11013403
申请日：2004-12-17
CPC分类号： C12Q1/6834 , G01N21/6452 , G01N21/6456 , G01N2201/062 , C12Q2565/619
摘要： An apparatus measures individual measurement sites on a DNA chip in a short period of time. The DNA chip is irradiated by light-emitting diodes (LEDs) so as to excite fluorescent dye at each measurement site, and fluorescence emitted from the individual measurement sites is detected all at once. Since substantially uniform measurement conditions can be obtained for each measurement site, measurement accuracy increases. The read mechanism requires less space and is less costly, thereby decreasing the failure rate and virtually eliminating the need for maintenance of the apparatus.
摘要翻译： 一种装置在短时间内测量DNA芯片上的各个测量位置。 DNA芯片被发光二极管（LED）照射，以便在每个测量部位激发荧光染料，并且一次检测到从各测量点发射的荧光。 由于每个测量点可以获得大致均匀的测量条件，所以测量精度提高。 读取机构需要更少的空间并且成本更低，从而降低故障率并且几乎消除了维护设备的需要。
-
公开(公告)号：US06572752B1
公开(公告)日：2003-06-03
申请号：US09671818
申请日：2000-09-27
IPC分类号： G01N27453
CPC分类号： G01N27/44713 , G01N27/447 , G01N27/44704 , G01N27/44743 , G01N27/44782
摘要： The troublesomeness during the setting of a plurality of capillaries is eliminated by composing pairs of electrodes, which are electrically connected to the common electrode, and capillaries. By bringing electrodes installed in the vicinity of each capillary disposed at the pitch of wells on the side of sample plate (within the area of the wells) into electrical contact with a common electrode, the capillaries and electrodes are made integral in construction. When a voltage is applied to the electrophoretic instrument via a common electrode portion, the voltage is applied to the electrodes for each capillary. This enables an inexpensive microtiter plate, etc. to be used and a multiple of capillaries to be simultaneously inserted, attached and detached.
摘要翻译： 通过构成电连接到公共电极的电极对和毛细管来消除在多个毛细管的设置期间的麻烦。通过将设置在每个毛细管附近的电极安装在位于 样品板（在孔的区域内）与公共电极电接触，毛细管和电极在结构上是一体的。 当通过公共电极部分向电泳仪施加电压时，对每个毛细管的电极施加电压。 这使得可以使用廉价的微量滴定板等，同时插入，附着和分离多个毛细管。
-
16.发明授权Sample processing device, sample treatment method, and reaction container used in these device and method 有权这些装置和方法中使用的样品处理装置，样品处理方法和反应容器公开(公告)号：US08906304B2
公开(公告)日：2014-12-09
申请号：US14122325
申请日：2012-05-21
申请人： Muneo Maeshima
发明人： Muneo Maeshima
CPC分类号： C12N15/1013 , G01N35/0099 , G01N35/02 , G01N35/026 , G01N35/10 , G01N35/1067 , G01N35/109 , G01N2035/103
摘要： Reaction containers (110) each comprising a plurality of treatment parts (wells) (501-506) are placed side by side in a reaction container set so as to be movable independently of each other in the direction of arrangement of the treatment parts (wells). A plurality of stems (401) correspond to the respective reaction containers (110) and are disposed above the reaction containers to be vertically movable and disposed in the direction crossing the direction of movement of the reaction containers. Control is performed so that when the reaction containers (110) and a stem mechanism (111) are operated together and one of the treatment parts (501-506) of each of the reaction containers (110) comes immediately below the stem mechanism (111) in accordance with a treatment procedure, a stem (401) corresponding thereto, a magnetic chip (402) attached thereto, or the cover (405) thereof can go into and out of the treatment part.
摘要翻译： 每个包括多个处理部分（孔）（501-506）的反应容器（110）并排放置在反应容器组中，以便可以在治疗部分（孔（well））的排列方向上彼此独立地移动 ）。 多个杆（401）对应于各个反应容器（110），并且设置在反应容器上方，以便可以沿与反应容器的移动方向交叉的方向垂直移动并设置。 进行控制，使得当反应容器（110）和阀杆机构（111）一起操作并且每个反应容器（110）的处理部件（501-506）中的一个立即在阀机构（111）的正下方 ），与其对应的杆（401），附接到其上的磁芯（402）或其盖（405）可以进出处理部。
-
公开(公告)号：US20140200162A1
公开(公告)日：2014-07-17
申请号：US14233256
申请日：2012-05-16
申请人： Toshiro Saito , Koshin Hamasaki , Satoshi Takahashi , Muneo Maeshima , Kyoko Imai , Kazumichi Imai , Ryuji Tao
发明人： Toshiro Saito , Koshin Hamasaki , Satoshi Takahashi , Muneo Maeshima , Kyoko Imai , Kazumichi Imai , Ryuji Tao
CPC分类号： C12Q1/6834 , C12N15/1065 , C12Q2525/207 , C12Q2537/125 , C12Q2537/143 , C12Q2563/143 , C12Q2563/149
摘要： A convenient method for nucleic acid analysis is provided, which enables 1000 or more types of nucleic acid to be analyzed collectively with high comprehensiveness and with a dynamic range of at least four digits. In particular, provided is a very effective analytical method especially for untranslated RNAs and microRNAs, of which the types of target nucleic acids is 10000 or lower. Nucleic acids can be analyzed conveniently and rapidly with high comprehensiveness and quantitative performance at single-molecule sensitivity and resolution by following the steps of: preparing a group of target nucleic acid fragments one molecule at a time and hybridizing the nucleic acid molecules, which have known base sequences and have been labeled with the fluorescence substances, with the group of the target nucleic acid fragments to detect the fluorescence substances labeling the hybridized nucleic acid molecules.
摘要翻译： 提供了一种方便的核酸分析方法，使得能够以高全面性和至少四位数的动态范围共同分析1000种或更多种类型的核酸。 特别地，提供了非常有效的分析方法，特别是对于非翻译的RNA和微小RNA，其靶核酸的类型为10000或更低。 可以通过以下步骤以单分子灵敏度和分辨率的高全面性和定量性能方便快速地分析核酸：一次一个分子制备一组靶核酸片段，并将已知的核酸分子杂交 碱基序列，并用荧光物质标记，用靶核酸片段组来检测标记杂交核酸分子的荧光物质。
-
18.发明申请公开(公告)号：US20130230908A1
公开(公告)日：2013-09-05
申请号：US13883830
申请日：2011-10-27
IPC分类号： B01L7/00
CPC分类号： B01L7/52 , B01L3/50851 , B01L3/50855 , B01L7/5255 , B01L2200/025 , B01L2300/0803 , B01L2300/0822 , G01N21/6456 , G01N21/6486 , G01N35/028 , G01N2035/00366 , G01N2035/0429 , G01N2035/0441 , G01N2035/0465
摘要： Provided is a technology such that, in nucleic acid analysis, a high degree of freedom in loading or unloading a reaction plate can be obtained and a sample can be efficiently analyzed. A reaction plate assembly includes a reaction plate with one or more reaction wells, a visible light transmissive cover mounted on the reaction plate and covering the reaction wells, and a visible light transmissive weight member covering the cover. The reaction wells are disposed in an arc shape along the circumference of a circle with a predetermined radius r1.
摘要翻译： 提供了一种技术，使得在核酸分析中，可以获得装载或卸载反应板的高自由度，并且可以有效地分析样品。 反应板组件包括具有一个或多个反应孔的反应板，安装在反应板上并覆盖反应孔的可见光透射盖和覆盖盖的可见光透射重量构件。 反应孔沿着具有预定半径r1的圆的圆周设置为弧形。
-
19.发明申请Sample Processing Device, Sample Processing Method, and Reaction Container Used in These Device and Method 有权这些装置和方法中使用的样品处理装置，样品处理方法和反应容器公开(公告)号：US20120309104A1
公开(公告)日：2012-12-06
申请号：US13516157
申请日：2010-12-08
申请人： Chihiro Uematsu , Muneo Maeshima
发明人： Chihiro Uematsu , Muneo Maeshima
CPC分类号： G01N35/10 , B01L3/0275 , B01L2200/0647 , B01L2200/143 , B01L2300/0829 , B01L2400/043 , G01N35/0098 , G01N2035/1048 , Y10T436/25
摘要： This invention concerns a sample processing device capable of efficiently recovering biological molecules, such as nucleic acids or proteins. The sample processing device is capable of placing a reaction container having a plurality of reaction sites, and it comprises a nozzle mechanism with a nozzle capable of attaching and removing a dispenser tip for dispensing a solution into the reaction sites of the reaction container and a magnetic tip for generating a magnetic field that allows magnetic beads to migrate to a space among the plurality of reaction sites in the reaction container, and a drive control unit controlling the nozzle mechanism.
摘要翻译： 本发明涉及能够有效地回收生物分子例如核酸或蛋白质的样品处理装置。 样品处理装置能够放置具有多个反应位置的反应容器，并且其包括具有喷嘴的喷嘴机构，喷嘴机构能够附接和移除用于将溶液分配到反应容器的反应位置的分配器末端和磁性 用于产生允许磁珠迁移到反应容器中的多个反应部位之间的空间的磁场的尖端，以及控制喷嘴机构的驱动控制单元。
-
20.发明授权公开(公告)号：US07540949B2
公开(公告)日：2009-06-02
申请号：US11188644
申请日：2005-07-26
IPC分类号： G01N27/453
CPC分类号： G01N27/44782 , G01N27/44704
摘要： At a wall constituting a space for a thermostatic oven in an electrophoresis apparatus, a capillary array attachment portion is formed which permits attachment of a plurality of capillary arrays having different length. Thereby, a selected capillary array constituted by collecting a plurality of capillaries can be easily attached to the electrophoresis apparatus depending on measurement purpose.
-
-
-
-
-
-
-
-
-
搜索结果
31 条记录 (耗时 0.028 秒)