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公开(公告)号：US08454977B2
公开(公告)日：2013-06-04
申请号：US13179058
申请日：2011-07-08
IPC分类号： A61K39/108 , A61K39/00 , A61K39/02
CPC分类号： A61K38/164 , A61L12/14 , A61L27/227 , A61L27/54 , A61L29/048 , A61L29/16
摘要： The present invention relates to a composition, device and method for preventing or inhibiting biofilm formation on biotic or abiotic surfaces. The composition comprises a peptide based on the C-terminal receptor binding domain of Pseudomonas aeruginosa type IV pilin, which binds to an abiotic surface (e.g., steel, plastic) with high affinity and prevents binding of a variety of P. aeruginosa strains to the surface. The inventive composition represents a non-toxic inhibitor for biofilm formation, particularly on an abiotic surface, which is responsible for a large number of problematic diseases and massive economic losses. The inventive method is useful as a safe and environmentally friendly means of modifying a surface of a variety of biomedical, nanotechnological, and biotechnological devices or articles.
摘要翻译： 本发明涉及在生物或非生物表面上预防或抑制生物膜形成的组合物，装置和方法。 组合物包含基于铜绿假单胞菌IV型皮林的C-末端受体结合结构域的肽，其以高亲和力结合非生物表面（例如，钢，塑料），并防止多种铜绿假单胞菌菌株与 表面。 本发明的组合物代表用于生物膜形成的无毒性抑制剂，特别是在非生物表面上，其负责大量有问题的疾病和巨大的经济损失。 本发明的方法可用作改变各种生物医学，纳米技术和生物技术装置或制品的表面的安全和环境友好的手段。
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公开(公告)号：US20110130344A1
公开(公告)日：2011-06-02
申请号：US13024187
申请日：2011-02-09
CPC分类号： C07K7/06 , A61K38/07 , A61K38/177
摘要： The invention provides tight junction protein modulators, compositions comprising the same, and uses thereof. In particular, the invention provides tight junction protein modulators that modulate the second extracellular loop of tight junction proteins, such as occludin or claudin.
摘要翻译： 本发明提供了紧密连接蛋白调节剂，包含其的组合物及其应用。 特别地，本发明提供了紧密连接蛋白调节剂，其调节紧密连接蛋白（例如闭合蛋白或紧密连接蛋白）的第二细胞外环。
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公开(公告)号：US06461490B1
公开(公告)日：2002-10-08
申请号：US09626775
申请日：2000-07-27
IPC分类号： G02N2726
CPC分类号： G01N33/5438 , B01J2219/00653 , B01J2219/00659 , B01J2219/00704 , B01J2219/00725 , C12Q1/003 , C12Q1/6825 , C40B40/10 , G01N33/54373 , Y10S436/908 , C12Q2565/607
摘要： A biosensor apparatus for detecting a binding event between a ligand and receptor. The apparatus includes a biosensor surface and surface-bound two-subunit heterodimer complexes composed of first and second, preferably oppositely charged peptides that together form an &agr;-helical coiled-coil heterodimer. The-first peptide is attached to the biosensor surface, and the second peptide carries the ligand, accessible for binding by a ligand-binding agent. Binding of anti-ligand binding agent to the surface-bound ligand is detected by a suitable detector. A ligand-specific biosensor surface can be readily prepared from a universal template containing the first charged peptide, by addition of a selected ligand attached to the second peptide.
摘要翻译： 一种用于检测配体和受体之间的结合事件的生物传感器装置。 该装置包括由第一和第二，优选相反电荷的肽组成的生物传感器表面和表面结合的双亚基异二聚体复合物，其一起形成α-螺旋卷曲螺旋二聚体。 第一个肽连接到生物传感器表面，第二个肽携带配体，可以通过配体结合剂结合。 通过合适的检测器检测抗配体结合剂与表面结合配体的结合。 配体特异性生物传感器表面可以容易地由含有第一带电荷肽的通用模板制备，通过加入附着于第二肽的选定配体。
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公开(公告)号：US6130037A
公开(公告)日：2000-10-10
申请号：US845505
申请日：1997-04-24
IPC分类号： C12Q1/00 , C40B40/10 , G01N33/543 , G01N27/00 , C12Q1/68 , G01N33/557 , A61L3/00
CPC分类号： C12Q1/003 , G01N33/54373 , G01N33/5438 , B01J2219/00653 , B01J2219/00659 , B01J2219/00704 , B01J2219/00725 , C40B40/10 , Y10S436/807
摘要： A biosensor apparatus for detecting a binding event between a ligand and receptor. The apparatus includes an electrode substrate coated with a high-dielectric hydrocarbon-chain monolayer, and having ligands attached to the exposed monolayer surface. Binding of a receptor to the monolayer-bound ligand, and the resultant perturbation of the monolayer structure, causes ion-mediated electron flow across the monolayer. In one embodiment, the monolayers have a coil-coil heterodimer embedded therein, one subunit of which is attached to the substrate, and the second of which carries the ligand at the monolayer surface.
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公开(公告)号：US5738996A
公开(公告)日：1998-04-14
申请号：US260199
申请日：1994-06-15
申请人： Robert S. Hodges , Randall T. Irvin , Arne Holm , Wah Y. Wong , Hasmukh B. Sheth , Devon L. Husband
发明人： Robert S. Hodges , Randall T. Irvin , Arne Holm , Wah Y. Wong , Hasmukh B. Sheth , Devon L. Husband
CPC分类号： C40B80/00 , B01J19/0046 , C07K1/047 , G01N33/53 , B01J2219/00315 , B01J2219/0059 , B01J2219/00592 , B01J2219/00596 , B01J2219/00605 , B01J2219/00612 , B01J2219/00621 , B01J2219/00626 , B01J2219/00659 , B01J2219/00725 , C40B40/10 , C40B60/14
摘要： A combinatorial library composition and method for using the library to construct oligomers effective to bind to a selected ligand is disclosed. The library composition includes at least two sets of ar oligomer libraries, each library set having selected oligomer subunit positions filled by known subunits, and other subunit positions containing permutations of subunits. In the selection method, oligomers from each library set are identified, and a new permutation library formed of subunits corresponding to the highest binding affinity oligomers in each library is screened for binding affinity to the selected ligand.
摘要翻译： 公开了组合文库组合物和使用文库构建有效结合选定配体的低聚物的方法。 文库组合物包括至少两组ar寡聚体文库，每个文库集合具有由已知亚基填充的选定的寡聚体亚单位，以及含有亚基排列的其它亚基位置。 在选择方法中，鉴定了来自每个文库的寡聚体，并筛选了与每个文库中最高结合亲和性寡聚体相应的亚基形成的新的置换文库，以对所选择的配体进行结合亲和力。
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16.发明授权
公开(公告)号：US5445818A
公开(公告)日：1995-08-29
申请号：US927797
申请日：1992-08-10
申请人： Robert S. Hodges , William Paranchych , Kok K. Lee , Sastry A. Parimi , Randall T. Irvin , Peter C. Doig
发明人： Robert S. Hodges , William Paranchych , Kok K. Lee , Sastry A. Parimi , Randall T. Irvin , Peter C. Doig
IPC分类号： A61K39/00 , A61K31/00 , A61K38/00 , A61K39/104 , A61K39/395 , A61P31/00 , A61P31/04 , C07K7/08 , C07K14/195 , C07K14/21 , C07K14/41 , C07K14/705 , C07K16/00 , C07K16/46 , C12N15/09 , C12P21/02 , G01N33/569 , A61K39/02 , A61K38/10 , A61K39/38 , C07K1/00
CPC分类号： C07K16/461 , C07K14/21 , A61K38/00 , A61K39/00 , C07K2319/00
摘要： Treatment and diagnosis of P. aeruginosa infection or colonization is achieved in accordance with this invention by the discovery of a polypeptide which is smaller than the naturally occurring P. aeruginosa pillin protein. The pure polypeptide comprises at least one amino acid residue sequence containing about twelve amino acid residues and up to about twenty amino acid residues that define a sequence capable of immunologically mimicking an antigenic determinant cite of P. aeruginosa pilin. This amino acid residue sequence can repeat as a unit one or more times in the same polypeptide molecule. More than one of such repeating units and more than one repeating unit of the same type can be present in a single polypeptide molecule. The polypeptides act an antigens or immunogens and antibodies may be raised to the immunogens and a vaccine prepared suitable for the prevention of P. aeruginosa infection.
摘要翻译： 根据本发明通过发现小于天然存在的铜绿假单胞菌丸蛋白的多肽实现铜绿假单胞菌感染或定植的治疗和诊断。 纯多肽包含至少一个含有约十二个氨基酸残基和至多约二十个氨基酸残基的氨基酸残基，其限定能够免疫模拟铜绿假单胞菌菌素的抗原决定簇的序列。 该氨基酸残基序列可以在同一多肽分子中作为单元重复一次或多次。 多于一个这样的重复单元和多于一个相同类型的重复单元可以存在于单个多肽分子中。 多肽起抗原或免疫原的作用，抗体可以产生于免疫原和适于预防铜绿假单胞菌感染的疫苗。
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公开(公告)号：US07976851B2
公开(公告)日：2011-07-12
申请号：US11996379
申请日：2006-07-21
IPC分类号： A61K39/108 , A61K39/00 , A61K39/02
CPC分类号： A61K38/164 , A61L12/14 , A61L27/227 , A61L27/54 , A61L29/048 , A61L29/16
摘要： The present invention relates to a composition and method for preventing or inhibiting biofilm formation on biotic or abiotic surfaces. The composition comprises a peptide based on the C-terminal receptor binding domain of Pseudomonas aeruginosa type IV pilin, which binds to an abiotic surface (e.g., steel, plastic) with high affinity and prevents binding of a variety of P. aeruginosa strains to the surface. The inventive composition represents a non-toxic inhibitor for biofilm formation, particularly on an abiotic surface, which is responsible for a large number of problematic diseases and massive economic losses. The inventive method is useful as a safe and environmentally friendly means of modifying a surface of a variety of biomedical, nanotechnological, and biotechnological devices or articles.
摘要翻译： 本发明涉及在生物或非生物表面上预防或抑制生物膜形成的组合物和方法。 组合物包含基于铜绿假单胞菌IV型皮林的C-末端受体结合结构域的肽，其以高亲和力结合非生物表面（例如，钢，塑料），并防止多种铜绿假单胞菌菌株与 表面。 本发明的组合物代表用于生物膜形成的无毒性抑制剂，特别是在非生物表面上，其负责大量有问题的疾病和巨大的经济损失。 本发明的方法可用作改变各种生物医学，纳米技术和生物技术装置或制品的表面的安全和环境友好的手段。
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公开(公告)号：US06541007B1
公开(公告)日：2003-04-01
申请号：US09345624
申请日：1999-06-30
IPC分类号： A61K39385
CPC分类号： A61K39/104 , A61K2039/505 , A61K2039/6037 , A61K2039/6081 , C07K14/21 , C07K2317/21 , Y10S530/806 , Y10S530/807 , Y10S530/825
摘要： A C-terminal pilin peptide vaccine for immunizing or treating a patient for infection by a Pseudomonas aeruginosa (PA) infection is disclosed. The peptide comprises the peptide identified as SEQ ID NOS. 3-6; and a carrier protein conjugated to the peptide. Also disclosed is a pilin peptide C-terminal PA pilin peptide having the amino acid sequence identified as SEQ ID NO:3, and analogs thereof having one of residues T, K, or A at position 130, D, T, or N at position 132, Q, A, or V at position 133, E, P, N, or A at position 135, Q, M, or K at position 136, and I, T, L, or R at position 138, excluding SEQ ID NOS: 1, 2, 9, 10, and 11, and the ability to cross-react with antibodies against the corresponding C-terminal peptides from PA strains PAK and PAO.
摘要翻译： 公开了用于免疫或治疗患者感染铜绿假单胞菌（PA）感染的C末端蛋白肽疫苗。 肽包含鉴定为SEQ ID NOS的肽。 3-6; 和与肽结合的载体蛋白。 还公开了具有鉴定为SEQ ID NO：3的氨基酸序列的pilin肽C-末端肽肽素，其类似物在位置130，D，T或N位置上具有残基T，K或A之一的位置 132位的Q，A或V位于第136位的第135位，第13位，第13位，第13位，第13位，第13位，第13位，第13位，第13位，第13位，第13位， NOS：1,2,9,10和11，以及与来自PA菌株PAK和PAO的相应C末端肽的抗体交叉反应的能力。
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公开(公告)号：US06478939B1
公开(公告)日：2002-11-12
申请号：US09578353
申请日：2000-05-24
IPC分类号： G01N2726
CPC分类号： G01N33/5438 , B01J2219/00653 , B01J2219/00659 , B01J2219/00704 , B01J2219/00725 , C12Q1/003 , C12Q1/6825 , C40B40/10 , G01N33/54373 , Y10S436/908 , C12Q2565/607
摘要： A biosensor apparatus for detecting a binding event between a ligand and receptor. The apparatus includes a biosensor surface and surface-bound two-subunit heterodimer complexes composed of first and second, preferably oppositely charged peptides that together form an &agr;-helical coiled-coil heterodimer. The first peptide is attached to the biosensor surface, and the second peptide carries the ligand, accessible for binding by a ligand-binding agent. Binding of anti-ligand binding agent to the surface-bound ligand is detected by a suitable detector. A ligand-specific biosensor surface can be readily prepared from a universal template containing the first charged peptide, by addition of a selected ligand attached to the second peptide.
摘要翻译： 一种用于检测配体和受体之间的结合事件的生物传感器装置。 该装置包括由第一和第二，优选相反电荷的肽组成的生物传感器表面和表面结合的双亚基异二聚体复合物，其一起形成α-螺旋卷曲螺旋二聚体。 第一个肽连接到生物传感器表面，第二个肽携带配体，可以通过配体结合剂结合。 通过合适的检测器检测抗配体结合剂与表面结合配体的结合。 配体特异性生物传感器表面可以容易地由含有第一带电荷肽的通用模板制备，通过加入附着于第二肽的选定配体。
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公开(公告)号：US6165335A
公开(公告)日：2000-12-26
申请号：US845660
申请日：1997-04-24
IPC分类号： C12Q1/00 , C12Q1/68 , C40B40/10 , G01N27/414 , G01N33/543 , G01N27/26 , G01N21/00
CPC分类号： G01N33/5438 , B01J2219/00653 , B01J2219/00659 , B01J2219/00704 , B01J2219/00725 , C12Q1/003 , C12Q1/6825 , C40B40/10 , G01N33/54373 , Y10S436/908 , C12Q2565/607
摘要： A biosensor apparatus for detecting a binding event between a ligand and receptor. The apparatus includes a biosensor surface and surface-bound two-subunit heterodimer complexes composed of first and second, preferably oppositely charged peptides that together form an .alpha.-helical coiled-coil heterodimer. The first peptide is attached to the biosensor surface, and the second peptide carries the ligand, accessible for binding by a ligand-binding agent. Binding of anti-ligand binding agent to the surface-bound ligand is detected by a suitable detector. A ligand-specific biosensor surface can be readily prepared from a universal template containing the first charged peptide, by addition of a selected ligand attached to the second peptide.
摘要翻译： 一种用于检测配体和受体之间的结合事件的生物传感器装置。 该装置包括生物传感器表面和表面结合的双亚基异二聚体复合物，其由第一和第二，优选相反电荷的肽组成，其一起形成α-螺旋线圈异二聚体。 第一个肽连接到生物传感器表面，第二个肽携带配体，可以通过配体结合剂结合。 通过合适的检测器检测抗配体结合剂与表面结合配体的结合。 配体特异性生物传感器表面可以容易地由含有第一带电荷肽的通用模板制备，通过加入附着于第二肽的选定配体。
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