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公开(公告)号：US08372416B2
公开(公告)日：2013-02-12
申请号：US12679158
申请日：2008-10-01
IPC分类号： A01N25/08
CPC分类号： C23C14/18 , A01N59/16 , B01J23/52 , B01J35/0013 , B01J37/0221 , C23C14/223 , A01N25/08 , A01N25/34 , A01N2300/00
摘要： A process for limiting the growth of microorganisms comprises (a) providing an antimicrobial agent comprising fine-nanoscale gold on a support medium comprising nanoparticulate titania, the fine-nanoscale gold having been deposited on the support medium by physical vapor deposition; and (b) contacting at least one microorganism with the antimicrobial agent.
摘要翻译： 限制微生物生长的方法包括（a）在包含纳米颗粒二氧化钛的载体介质上提供包含微细纳米级金的抗微生物剂，所述微细纳米金已通过物理气相沉积沉积在载体介质上; 和（b）使至少一种微生物与抗微生物剂接触。
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公开(公告)号：US20120003626A1
公开(公告)日：2012-01-05
申请号：US13142288
申请日：2009-12-30
IPC分类号： C12Q1/04 , C12N1/20 , C12N1/14 , C12N1/10 , C12M1/26 , C12M1/34 , C12Q1/70 , C12N7/02 , C12N1/16 , B82Y15/00
摘要： The present disclosure describes methods for concentrating microorganisms with concentration agents in a sampling device and the sampling device described herein. More specifically, methods for concentrating microorganisms from large volume samples with concentration agents in a sampling device can provide for rapid, low cost, simple (involving no complex equipment or procedures), and/or effective processes under a variety of conditions.
摘要翻译： 本公开描述了将浓缩剂浓缩在采样装置中的微生物和本文所述的采样装置的方法。 更具体地说，用于将来自大容量样品的微生物浓缩在采样装置中的浓缩剂的方法可以在各种条件下提供快速，低成本，简单（不涉及复杂的设备或程序）和/或有效过程。
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公开(公告)号：US20100209961A1
公开(公告)日：2010-08-19
申请号：US12678579
申请日：2008-10-02
CPC分类号： C12N11/14 , B01J20/02 , B01J20/06 , B01J20/14 , B01J20/3204 , B01J20/3234 , B01J20/3236 , C12N1/02 , C12Q1/04 , C12Q1/24
摘要： A process for capturing or concentrating microorganisms for detection or assay comprises (a) providing a concentration agent that comprises diatomaceous earth bearing, on at least a portion of its surface, a surface treatment comprising a surface modifier comprising titanium dioxide, fine-nanoscale gold or platinum, or a combination thereof; (b) providing a sample comprising at least one microorganism strain; and (c) contacting the concentration agent with the sample such that at least a portion of the at least one microorganism strain is bound to or captured by the concentration agent.
摘要翻译： 用于捕获或浓缩用于检测或测定的微生物的方法包括（a）在其表面的至少一部分上提供包含硅藻土的浓缩剂，所述表面处理包括表面改性剂，其包含二氧化钛，细纳米级金或 铂或其组合; （b）提供包含至少一种微生物菌株的样品; 和（c）使浓缩剂与样品接触，使得至少一部分微生物菌株与浓缩剂结合或捕获。
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14.发明申请公开(公告)号：US20100062421A1
公开(公告)日：2010-03-11
申请号：US12597326
申请日：2008-04-25
CPC分类号： C12N15/1006 , G01N33/569
摘要： Compositions, methods, devices, and kits, which include an immobilized-metal support material comprising a substrate having a plurality of —C(O)O− or —P(O)(—OH)2-x(—O−)x groups bound to the substrate and a plurality of metal ions, My+, bound to the —C(O)O− or —P(O)(—OH)2-x(—O−)x groups; wherein M is selected from the group consisting of zirconium, gallium, iron, aluminum, scandium, titanium, vanadium, yttrium, and a lanthanide; y is an integer from 3 to 6; and x is 1 or 2, and to which microorganisms and polynucleotides bind, and which can be used for separating and optionally assaying microorganisms and/or a polynucleotide from a sample material are disclosed.
摘要翻译： 组合物，方法，装置和试剂盒，其包括固定金属载体材料，其包含具有多个-C（O）O-或-P（O）（-OH）2-x（-O-）x 结合到基底和与-C（O）O-或-P（O）（ - OH）2-x（-O-）x基团结合的多个金属离子My +的基团; 其中M选自锆，镓，铁，铝，钪，钛，钒，钇和镧系元素; y为3〜6的整数; 并且x为1或2，并且公开了微生物和多核苷酸结合，并且其可用于从样品材料分离和任选地测定微生物和/或多核苷酸。
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公开(公告)号：US09284593B2
公开(公告)日：2016-03-15
申请号：US13519270
申请日：2010-12-30
CPC分类号： C12Q1/04 , B01L3/502 , B01L3/5029 , B01L3/5082 , B01L2300/042 , B01L2300/046 , B01L2300/0672 , B01L2300/087 , B01L2400/0478 , B01L2400/0644 , B01L2400/0683 , G01N33/5005
摘要： The present invention provides methods to concentrate cells onto microparticles, to concentrate the microparticles, and to detect the cells. The present invention also includes unitary sample preparation and detection devices to be used in accordance with the methods.
摘要翻译： 本发明提供了将细胞浓缩到微粒上，浓缩微粒并检测细胞的方法。 本发明还包括根据该方法使用的单一样品制备和检测装置。
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公开(公告)号：US08741595B2
公开(公告)日：2014-06-03
申请号：US13142455
申请日：2009-12-29
IPC分类号： C12Q1/04
CPC分类号： C12Q1/04 , C12Q1/10 , C12Q1/24 , G01N33/56916 , G01N2520/00
摘要： A process comprises (a) providing (1) at least one sample suspected of comprising at least one coliform strain, (2) at least one culture device comprising at least one culture medium that is hydrated or hydratable, and (3) at least one particulate concentration agent that is substantially optically transparent when in contact with the culture medium in the culture device when the culture medium is hydrated; (b) placing the particulate concentration agent in contact with the sample such that at least a portion of the coliform strain is bound to the particulate concentration agent to form coliform-bound particulate concentration agent; (c) placing the coliform-bound particulate concentration agent in contact with the culture medium; (d) incubating the culture device comprising the coliform-bound particulate concentration agent in contact with the culture medium, the culture medium being hydrated; and (e) optically detecting the presence of the coliform strain without separating the coliform strain from the particulate concentration agent.
摘要翻译： 一种方法包括（a）提供（1）至少一种怀疑含有至少一种大肠杆菌菌株的样品，（2）至少一种包含至少一种水合或可水合的培养基的培养装置，和（3）至少一种 当培养基水合时，当与培养装置中的培养基接触时基本上是光学透明的颗粒浓缩剂; （b）使颗粒浓缩剂与样品接触，使得至少一部分大肠杆菌菌株与颗粒物浓缩剂结合形成大肠菌群结合的颗粒浓缩剂; （c）使大肠杆菌结合的颗粒浓缩剂与培养基接触; （d）将包含大肠杆菌结合的颗粒浓缩剂的培养装置与培养基接触培养，培养基水合; 和（e）光学检测大肠菌群的存在，而不从大颗粒浓缩剂中分离大肠杆菌菌株。
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公开(公告)号：US20130029324A1
公开(公告)日：2013-01-31
申请号：US13519270
申请日：2010-12-30
CPC分类号： C12Q1/04 , B01L3/502 , B01L3/5029 , B01L3/5082 , B01L2300/042 , B01L2300/046 , B01L2300/0672 , B01L2300/087 , B01L2400/0478 , B01L2400/0644 , B01L2400/0683 , G01N33/5005
摘要： The present invention provides methods to concentrate cells onto microparticles, to concentrate the microparticles, and to detect the cells. The present invention also includes unitary sample preparation and detection devices to be used in accordance with the methods.
摘要翻译： 本发明提供了将细胞浓缩到微粒上，浓缩微粒并检测细胞的方法。 本发明还包括根据该方法使用的单一样品制备和检测装置。
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公开(公告)号：US20110269179A1
公开(公告)日：2011-11-03
申请号：US13142455
申请日：2009-12-29
IPC分类号： C12Q1/04
CPC分类号： C12Q1/04 , C12Q1/10 , C12Q1/24 , G01N33/56916 , G01N2520/00
摘要： A process comprises (a) providing (1) at least one sample suspected of comprising at least one coliform strain, (2) at least one culture device comprising at least one culture medium that is hydrated or hydratable, and (3) at least one particulate concentration agent that is substantially optically transparent when in contact with the culture medium in the culture device when the culture medium is hydrated; (b) placing the particulate concentration agent in contact with the sample such that at least a portion of the coliform strain is bound to the particulate concentration agent to form coliform-bound particulate concentration agent; (c) placing the coliform-bound particulate concentration agent in contact with the culture medium; (d) incubating the culture device comprising the coliform-bound particulate concentration agent in contact with the culture medium, the culture medium being hydrated; and (e) optically detecting the presence of the coliform strain without separating the coliform strain from the particulate concentration agent.
摘要翻译： 一种方法包括（a）提供（1）至少一种怀疑含有至少一种大肠杆菌菌株的样品，（2）至少一种包含至少一种水合或可水合的培养基的培养装置，和（3）至少一种 当培养基水合时，当与培养装置中的培养基接触时基本上是光学透明的颗粒浓缩剂; （b）使颗粒浓缩剂与样品接触，使得至少一部分大肠杆菌菌株与颗粒物浓缩剂结合形成大肠菌群结合的颗粒浓缩剂; （c）使大肠杆菌结合的颗粒浓缩剂与培养基接触; （d）将包含大肠杆菌结合的颗粒浓缩剂的培养装置与培养基接触培养，培养基水合; 和（e）光学检测大肠菌群的存在，而不从大颗粒浓缩剂中分离大肠杆菌菌株。
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公开(公告)号：US20110143334A1
公开(公告)日：2011-06-16
申请号：US12808317
申请日：2008-12-18
CPC分类号： C12Q1/10 , C12M25/06 , C12M41/36 , C12Q1/04 , C12Q1/06 , C12Q1/24 , G01N2333/245 , Y02A50/54
摘要： Methods and systems for detecting the presence of a target microorganism in a liquid sample are provided. Methods comprise the steps of passing the liquid sample through a surface filter, placing the surface filter into contact with a culture device, incubating the culture device for a period of time and detecting the presence of a target microorganism. Methods may be used with an automated detection system.
摘要翻译： 提供了用于检测液体样品中目标微生物的存在的方法和系统。 方法包括使液体样品通过表面过滤器，使表面过滤器与培养装置接触，培养培养装置一段时间并检测目标微生物的存在的步骤。 方法可以与自动检测系统一起使用。
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公开(公告)号：US20100190171A1
公开(公告)日：2010-07-29
申请号：US12678362
申请日：2008-10-02
CPC分类号： G01N33/54313 , C12N1/02 , C12N11/14 , C12Q1/04 , C12Q1/24 , G01N33/552 , G01N33/553
摘要： A process for capturing or concentrating microorganisms for detection or assay comprises (a) providing a concentration agent that comprises an amorphous metal silicate and that has a surface composition having a metal atom to silicon atom ratio of less than or equal to about 0.5, as determined by X-ray photoelectron spectroscopy (XPS); (b) providing a sample comprising at least one microorganism strain; and (c) contacting the concentration agent with the sample such that at least a portion of the at least one microorganism strain is bound to or captured by the concentration agent.
摘要翻译： 用于捕获或浓缩用于检测或测定的微生物的方法包括（a）提供包含无定形金属硅酸盐并且具有小于或等于约0.5的金属原子与硅原子比的表面组成的浓度试剂，如所确定的 通过X射线光电子能谱（XPS）; （b）提供包含至少一种微生物菌株的样品; 和（c）使浓缩剂与样品接触，使得至少一部分微生物菌株与浓缩剂结合或捕获。
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