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公开(公告)号：US11473094B2
公开(公告)日：2022-10-18
申请号：US17317255
申请日：2021-05-11
Applicant: Monsanto Technology LLC
Inventor： Whitney R. Adams, Jr. , Brian J. Martinell , Jyoti R. Rout , Edward J. Williams
IPC: C12N15/82
Abstract: The present invention provides methods for improving competency of plant cells for bacterial-mediated transformation comprising contacting the plant cells with an effective amount of polyethylene glycol (PEG) for a period of time prior to transformation. The ability to store and maintain competent plant cells for transformation and tissue culture allows more efficient planning and execution of large-scale experiments by providing flexibility of peak production hours, or during unplanned disruptions in the production process. These methods are useful in preserving the viability of plant cells in various storage conditions, thus improving their competency for transformation and tissue culture.
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公开(公告)号：US20220119826A1
公开(公告)日：2022-04-21
申请号：US17520352
申请日：2021-11-05
Applicant: Monsanto Technology LLC
Inventor： Beth Jo Calabotta , Kevin Lee Depperman , Erik D. Dersch , Jerald D. Heise , Angela Ranae Koestel , Cindy L. Ludwig , Brian J. Martinell , Edward Williams
Abstract: The present invention relates to excision of explant material comprising meristematic tissue from seeds, and storage of such material prior to subsequent use in plant tissue culture and genetic transformation. Methods for tissue preparation, storage, and transformation are disclosed, as is transformable meristem tissue produced by such methods, and apparati for tissue preparation.
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公开(公告)号：US20210219508A1
公开(公告)日：2021-07-22
申请号：US17124032
申请日：2020-12-16
Applicant: Monsanto Technology LLC
Inventor： Whitney R. Adams, JR. , Brandon Davis , Lubomyr Kucher , Brian J. Martinell , Jyoti R. Rout , Brenda A. Lowe
IPC: A01H4/00
Abstract: The present invention provides methods and devices for the rapid isolation of monocot plant embryos suitable for transformation or tissue culture. The invention includes mechanical devices for substantially isolating plant embryos for use as transformable explants. Media suitable for isolating plant embryos and methods for their preparation are also provided.
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公开(公告)号：US10717983B2
公开(公告)日：2020-07-21
申请号：US15826540
申请日：2017-11-29
Applicant: Monsanto Technology, LLC
Inventor： Erik Dersch , Richard J. Heinzen , Brian J. Martinell , Anatoly Rivlin , Yuechun Wan , Xudong Ye
Abstract: The present invention relates to excision of explant material comprising meristematic tissue from cotton seeds. Methods for tissue preparation, storage, transformation, and selection or identification of transformed plants are disclosed, as are transformable meristem tissues and plants produced by such methods, and apparati for tissue preparation.
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公开(公告)号：US20190382777A1
公开(公告)日：2019-12-19
申请号：US16393833
申请日：2019-04-24
Applicant: MONSANTO TECHNOLOGY LLC
Inventor： Brian J. Martinell , Michael W. Petersen , David A. Somers , Yuechun Wan , Edward Williams , Xudong Ye
Abstract: The present invention relates to methods and compositions for transforming soybean, corn, cotton, or canola explants using spectinomycin as a selective agent for transformation of the explants. The method may further comprise treatment of the explants with cytokinin during the transformation and regeneration process.
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Patent Agency Ranking
公开(公告)号：US20170342426A1
公开(公告)日：2017-11-30
申请号：US15670668
申请日：2017-08-07
Applicant: Monsanto Technology LLC
Inventor： Toni Armstrong , Kenneth Barton , Laura J. Crow , Larry A. Gilbertson , Shihshieh Huang , Yong Huang , Brian J. Martinell , Michael W. Petersen , Kenneth J. Piller , Qi Wang , Xudong Ye
IPC: C12N15/82
CPC classification number: C12N15/8205 , C12N15/8209 , C12N15/8263 , C12N15/8265 , C12N15/8289
Abstract: The compositions and methods are provided that enhance the selection of transgenic plants having two T-DNA molecules integrated into a plant genome at different physical and genetic loci. The compositions are DNA constructs that comprise novel arrangements of T-DNA molecules containing genes of interest, positive selectable marker genes, and conditional lethal genes. The methods disclosed herein comprises transforming a plant cell to comprise the DNA constructs of the present invention, regenerating the plant cell into a plant and identifying independant transgene loci, where the selectable marker genes or transgenic elements can be segregated in the progeny.
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公开(公告)号：US20140096278A1
公开(公告)日：2014-04-03
申请号：US14077961
申请日：2013-11-12
Applicant: Monsanto Technology, LLC
Inventor： Jennifer R. Hicks , Brenda A. Lowe , Brian J. Martinell , John Phillips , John P. Tamulonis , Edward J. Williams
CPC classification number: C12N15/8289 , A01H1/04 , A01H5/00 , C12N15/8201 , C12N15/8205 , C12N15/8245 , C12N15/8247 , C12N15/8274 , C12N15/8281 , C12N15/8282 , C12N15/8283 , C12N15/8286
Abstract: The invention is directed, in an embodiment, to a method for producing a transgenic plant comprising: providing a substantially homozygous plant line suitable for transformation; selecting a subline of the plant line having reduced heterogeneity; transforming plant materials from the subline with a transgenic construct that confers a desired trait to at least one transformed plant; recovering at least one transgenic event from the transformation step; and selecting a transgenic event exhibiting a desirable level of the desired trait using plants of the subline as control. The invention is also directed to a method for producing a plant having a desired trait. The invention also provides transgenic plants produced according to these methods.
Abstract translation: 本发明在一个实施方案中涉及生产转基因植物的方法，其包括：提供适于转化的基本上纯合的植物系; 选择具有减小的异质性的植物系的一个子系; 用具有将所需性状赋予至少一种转化植物的转基因构建体从该亚系转化植物材料; 从转化步骤回收至少一个转基因事件; 并且使用该子系的植物作为对照选择表现出期望性状的期望水平的转基因事件。 本发明还涉及一种用于生产具有所需性状的植物的方法。 本发明还提供根据这些方法生产的转基因植物。
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公开(公告)号：US20230159942A1
公开(公告)日：2023-05-25
申请号：US18071348
申请日：2022-11-29
Applicant: Monsanto Technology LLC
Inventor： Beth Jo Calabotta , Kevin Lee Deppermann , Erik D. Dersch , Jerald D. Heise , Angela Ranae Koestel , Cindy L. Ludwig , Brian J. Martinell , Edward Williams
CPC classification number: C12N15/8209 , C12N5/0025 , C12N15/8221 , C12N15/8265 , A01H4/003 , A01H4/008 , C12N5/04 , C12N15/8202 , C12N15/8271 , C12N15/8281 , G01N33/0098 , C12N15/8201 , C12N9/1029 , C12N15/8205 , C12N15/8274 , C12N15/8275 , C12N15/8277 , C12N15/8261 , A01H6/202
Abstract: The present invention relates to excision of explant material comprising meristematic tissue from seeds, and storage of such material prior to subsequent use in plant tissue culture and genetic transformation. Methods for tissue preparation, storage, and transformation are disclosed, as is transformable meristem tissue produced by such methods, and apparati for tissue preparation.
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公开(公告)号：US11091767B2
公开(公告)日：2021-08-17
申请号：US16516025
申请日：2019-07-18
Applicant: MONSANTO TECHNOLOGY LLC
Inventor： Whitney R. Adams, Jr. , Brian J. Martinell , Jyoti R. Rout , Edward J. Williams
IPC: C12N15/82
Abstract: The present invention provides methods for improving competency of plant cells, such as corn plant immature embryo for bacterial-mediated transformation comprising contacting the plant cells, such as corn plant immature embryo with an effective amount of polyethylene glycol (PEG) for a period of time prior to transformation. The ability to store and maintain competent plant cells, such as corn plant immature embryo for transformation and tissue culture allows more efficient planning and execution of large-scale experiments by providing flexibility of peak production hours, or during unplanned disruptions in the production process. These methods are useful in preserving the viability of plant cells, such as corn plant immature embryo in various storage conditions, thus improving their competency for transformation and tissue culture.
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公开(公告)号：US10907167B2
公开(公告)日：2021-02-02
申请号：US16393833
申请日：2019-04-24
Applicant: MONSANTO TECHNOLOGY LLC
Inventor： Brian J. Martinell , Michael W. Petersen , David A. Somers , Yuechun Wan , Edward Williams , Xudong Ye
IPC: C12N15/82 , C12N15/84 , C12N15/62 , A01H1/04 , C12N9/10 , A01H4/00 , A01H6/20 , A01H6/46 , A01H6/54 , A01H6/60 , C12N5/00 , C12R1/41
Abstract: The present invention relates to methods and compositions for transforming soybean, corn, cotton, or canola explants using spectinomycin as a selective agent for transformation of the explants. The method may further comprise treatment of the explants with cytokinin during the transformation and regeneration process.
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