公开(公告)号：US20170067076A1

公开(公告)日：2017-03-09

申请号：US15348839

申请日：2016-11-10

Applicant: Monsanto Technology LLC

Inventor： Edwards Allen ,  William P. Donovan ,  Gregory R. Heck ,  James K. Roberts ,  Virginia Ursin ,  Yuanji Zhang

IPC: C12N15/82 ,  C12N15/113

Abstract: This invention provides plants having resistance to invertebrate pests. More specifically, this invention discloses a non-natural transgenic plant cell expressing at least one invertebrate miRNA in planta for suppression of a target gene of an invertebrate pest or of a symbiont associated with the invertebrate pest. Also provided are recombinant DNA constructs for expression of at least one invertebrate miRNA in planta, a non-natural transgenic plant containing the non-natural transgenic plant cell of this invention, a non-natural transgenic plant grown from the non-natural transgenic plant cell of this invention, and non-natural transgenic seed produced by the non-natural transgenic plants, as well as commodity products produced from a non-natural transgenic plant cell, plant, or seed of this invention. This invention further provides a method of suppressing at least one target gene of an invertebrate pest of a plant or of a symbiont associated with the invertebrate, including providing a plant including the non-natural transgenic plant cell of this invention, wherein the invertebrate is the invertebrate pest, the recombinant DNA is transcribed in the non-natural transgenic plant cell to the recombinant miRNA precursor, and when the invertebrate pest ingests the recombinant miRNA precursor, the at least one target gene is suppressed.

Abstract translation: 本发明提供具有抗无脊椎害虫的植物。 更具体地，本发明公开了一种非天然转基因植物细胞，其在植物中表达至少一种无脊椎动物miRNA，用于抑制无脊椎害虫或与无脊椎害虫相关的共生体的靶基因。 还提供了用于在植物中表达至少一种无脊椎动物miRNA的重组DNA构建体，包含本发明的非天然转基因植物细胞的非天然转基因植物，从非天然转基因植物细胞生长的非天然转基因植物 以及由非天然转基因植物产生的非天然转基因种子，以及由本发明的非天然转基因植物细胞，植物或种子产生的商品。 本发明还提供一种抑制植物无脊椎动物害虫或与无脊椎动物相关共生体的至少一种靶基因的方法，包括提供包含本发明非天然转基因植物细胞的植物，其中无脊椎动物是 无脊椎动物害虫，重组DNA在非天然转基因植物细胞中转录至重组miRNA前体，当无脊椎害虫摄入重组miRNA前体时，抑制至少一种靶基因。

公开(公告)号：US20160208283A1

公开(公告)日：2016-07-21

申请号：US14720052

申请日：2015-05-22

Applicant: Monsanto Technology LLC

Inventor： Jintai Huang ,  Sergey Ivashuta ,  Youlin Qi ,  Barbara Elizabeth Wiggins ,  Yuanji Zhang

IPC: C12N15/82 ,  A01N43/90

CPC classification number: C12N15/8289 ,  A01N37/46 ,  A01N43/90 ,  A01N65/44 ,  C12N15/8218 ,  C12N15/8275 ,  A01N57/20

Abstract: The invention provides methods and compositions for selectively suppressing the expression of a recombinant protein in a male reproductive tissue of a transgenic plant. The invention also provides methods and compositions for inducing male sterility in a transgenic plant. Plants, plant cells, plant parts, seeds, and commodity products including such compositions are aspects of the invention.

Abstract translation: 本发明提供用于选择性抑制转基因植物雄性生殖组织中重组蛋白表达的方法和组合物。 本发明还提供了用于在转基因植物中诱导雄性不育的方法和组合物。 包括这种组合物的植物，植物细胞，植物部分，种子和商品，是本发明的一个方面。

公开(公告)号：US09315819B2

公开(公告)日：2016-04-19

申请号：US14191825

申请日：2014-02-27

Applicant: Monsanto Technology LLC

Inventor： Edwards M. Allen ,  Sara E. Heisel ,  Sergey Ivashuta ,  Elysia K. Krieger ,  Jennifer Lutke ,  Robert J. Meister ,  Yuanji Zhang

IPC: C12N15/00 ,  C12N15/82

CPC classification number: C12N15/8216 ,  C12N15/8217 ,  C12N15/8218 ,  C12N15/8234 ,  C12N15/8238 ,  C12N15/8254 ,  C12N15/8265 ,  C12N15/827 ,  C12N15/8275 ,  C12N15/8279 ,  C12N15/8285 ,  C12N15/8286 ,  C12N15/8289 ,  Y02A40/162 ,  Y02A40/164

Abstract: This invention provides molecular constructs and methods for the temporally specific control of gene expression in plants or in plant pests or pathogens. More specifically, this invention provides plant miRNA genes having novel circadian expression patterns that are useful for designing recombinant DNA constructs for temporally specific expression of at least one gene. Also provided are non-natural transgenic plant cells, plants, and seeds containing in their genome a recombinant DNA construct of this invention.

Abstract translation: 本发明提供用于在植物或植物害虫或病原体中时间特异性控制基因表达的分子构建体和方法。 更具体地，本发明提供具有新颖的昼夜节律表达模式的植物miRNA基因，其可用于设计用于至少一种基因的时间特异性表达的重组DNA构建体。 还提供了在其基因组中含有本发明的重组DNA构建体的非天然转基因植物细胞，植物和种子。

公开(公告)号：US10745712B2

公开(公告)日：2020-08-18

申请号：US15660660

申请日：2017-07-26

Applicant: Monsanto Technology LLC

Inventor： Clayton T. Larue ,  Joel E. Ream ,  Aabid Shariff ,  Yuanji Zhang ,  Xuefeng Zhou

IPC: C12N15/82 ,  C07K14/415 ,  C12N9/02

Abstract: The invention provides recombinant DNA molecules useful for providing efficient expression of proteins in transgenic plants, as well as compositions and methods for using the recombinant DNA molecules. In particular embodiments, the invention provides recombinant DNA molecules and constructs comprising sequences encoding transit peptides and operably linked sequences conferring herbicide tolerance.

公开(公告)号：US10689667B2

公开(公告)日：2020-06-23

申请号：US15726363

申请日：2017-10-05

Applicant: Monsanto Technology LLC

Inventor： Jintai Huang ,  Sergey Ivashuta ,  Youlin Qi ,  Barbara Elizabeth Wiggins ,  Yuanji Zhang

IPC: C12N15/82 ,  A01H5/10

Abstract: The invention provides methods and compositions for selectively suppressing the expression of a recombinant protein in a male reproductive tissue of a transgenic plant. The invention also provides methods and compositions for inducing male sterility in a transgenic plant. Plants, plant cells, plant parts, seeds, and commodity products including such compositions are aspects of the invention.

公开(公告)号：US20180171350A1

公开(公告)日：2018-06-21

申请号：US15826546

申请日：2017-11-29

Applicant: Monsanto Technology, LLC

Inventor： Isaac Richard Banks ,  Sergey Ivashuta ,  Barbara Elizabeth Wiggins ,  Yuanji Zhang

IPC: C12N15/82

CPC classification number: C12N15/8262 ,  C12N15/8218 ,  C12N15/8261 ,  Y02A40/146

Abstract: Described herein are transgenic soybean chromosomes containing a recombinant DNA that transcribes to an RNA molecule that hybridizes to and forms a cleavage-resistant duplex with either a mature miR171 miRNA or a transcript of a target gene having a recognition site for a mature miR171 miRNA whereby the function of the miR171 miRNA is inhibited and thereby imparts enhanced agronomic traits to soybean plants such as increased pods per node, increased number of nodes, a decreased distance between nodes, and a twisted stem.

公开(公告)号：US20150247154A1

公开(公告)日：2015-09-03

申请号：US14691281

申请日：2015-04-20

Applicant: Monsanto Technology LLC

Inventor： Sergey I. Ivashuta ,  Barbara E. Wiggins ,  Yuanji Zhang

IPC: C12N15/82

CPC classification number: C12N15/8218 ,  C12N15/111 ,  C12N15/8216 ,  C12N15/8261 ,  C12N2310/141 ,  Y02A40/146

Abstract: This invention provides recombinant DNA constructs and methods for manipulating expression of a target gene that is regulated by a small RNA, by interfering with the binding of the small RNA to its target gene. More specifically, this invention discloses recombinant DNA constructs encoding cleavage blockers, 5-modified cleavage blockers, and translational inhibitors useful for modulating expression of a target gene and methods for their use. Further disclosed are miRNA targets useful for designing recombinant DNA constructs including miRNA-unresponsive transgenes, miRNA decoys, cleavage blockers, 5-modified cleavage blockers, and translational inhibitors, as well as methods for their use, and transgenic eukaryotic cells and organisms containing such constructs.

Abstract translation: 本发明通过干扰小RNA与其靶基因的结合来提供重组DNA构建体和操纵由小RNA调节的靶基因表达的方法。 更具体地，本发明公开了编码切割阻断剂的重组DNA构建体，5-修饰的切割阻断剂和用于调节靶基因表达的翻译抑制剂及其使用方法。 进一步公开的是可用于设计重组DNA构建体的miRNA靶标，包括miRNA-无反应转基因，miRNA诱饵，切割阻断剂，5-修饰的切割阻断剂和翻译抑制剂，以及其使用方法，以及含有这种构建体的转基因真核细胞和生物 。

公开(公告)号：US20150166993A1

公开(公告)日：2015-06-18

申请号：US14567786

申请日：2014-12-11

Applicant: Monsanto Technology LLC

Inventor： Edwards M. Allen ,  Barry S. Goldman ,  Liang Guo ,  Sara E. Heisel ,  Shihshieh Huang ,  Sergey I. Ivashuta ,  David K. Kovalic ,  Elysia K. Krieger ,  James K. Roberts ,  Yuanji Zhang

IPC: C12N15/113 ,  C12N15/82

CPC classification number: C12N15/113 ,  C12N15/8216 ,  C12N15/8218 ,  C12N15/8238 ,  C12N15/8261 ,  C12N15/8271 ,  C12N15/8273 ,  C12N15/8279 ,  C12N15/8286 ,  C12N2310/141 ,  C12N2320/50 ,  Y02A40/146 ,  Y02A40/162

Abstract: This invention discloses novel microRNAs and their precursors, and recombinant DNA constructs including such novel miRNAs, miRNA precursors, miRNA promoters, and miRNA recognition sites corresponding to the miRNAs. Included are novel miRNA and miRNA precursors that exhibit nutrient-responsive expression. Also disclosed are miRNA decoy sequences. Further provided are non-natural transgenic plant cells, plants, and seeds containing in their genome a recombinant DNA construct of this invention and methods of controlling gene expression using recombinant DNA constructs of this invention.

Abstract translation: 本发明公开了新的微小RNA及其前体，以及重组DNA构建体，包括这样的新型miRNA，miRNA前体，miRNA启动子和对应于miRNA的miRNA识别位点。 包括显示营养反应表达的新型miRNA和miRNA前体。 还公开了miRNA诱饵序列。 还提供了非天然转基因植物细胞，植物和种子，在其基因组中含有本发明的重组DNA构建体和使用本发明的重组DNA构建体控制基因表达的方法。

公开(公告)号：US20140317780A1

公开(公告)日：2014-10-23

申请号：US14191825

申请日：2014-02-27

Applicant: Monsanto Technology LLC

Inventor： Edwards M. Allen ,  Sara E. Heisel ,  Sergey Ivashuta ,  Elysia K. Krieger ,  Jennifer Lutke ,  Robert J. Meister ,  Yuanji Zhang

IPC: C12N15/82

CPC classification number: C12N15/8216 ,  C12N15/8217 ,  C12N15/8218 ,  C12N15/8234 ,  C12N15/8238 ,  C12N15/8254 ,  C12N15/8265 ,  C12N15/827 ,  C12N15/8275 ,  C12N15/8279 ,  C12N15/8285 ,  C12N15/8286 ,  C12N15/8289 ,  Y02A40/162 ,  Y02A40/164

Abstract: This invention provides molecular constructs and methods for the temporally specific control of gene expression in plants or in plant pests or pathogens. More specifically, this invention provides plant miRNA genes having novel circadian expression patterns that are useful for designing recombinant DNA constructs for temporally specific expression of at least one gene. Also provided are non-natural transgenic plant cells, plants, and seeds containing in their genome a recombinant DNA construct of this invention.

Abstract translation: 本发明提供用于在植物或植物害虫或病原体中时间特异性控制基因表达的分子构建体和方法。 更具体地，本发明提供具有新颖的昼夜节律表达模式的植物miRNA基因，其可用于设计用于至少一种基因的时间特异性表达的重组DNA构建体。 还提供了在其基因组中含有本发明的重组DNA构建体的非天然转基因植物细胞，植物和种子。

公开(公告)号：US11866721B2

公开(公告)日：2024-01-09

申请号：US16844949

申请日：2020-04-09

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Jintai Huang ,  Youlin Qi ,  Heping Yang ,  Yuanji Zhang

IPC: C12N15/82 ,  C12N9/10

CPC classification number: C12N15/8275 ,  C12N9/1092 ,  C12N15/8218 ,  C12N15/8231 ,  C12N15/8274 ,  C12N15/8289 ,  C12Y205/01019

Abstract: The invention provides novel recombinant DNA molecules, compositions, and methods for selectively regulating the expression of a transcribable polynucleotide molecule or recombinant protein in a male reproductive tissue of a transgenic plant. The invention also provides transgenic plants, plant cells, plant parts, seeds, and commodity products comprising such DNA molecules and compositions.