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公开(公告)号：US06656740B1
公开(公告)日：2003-12-02
申请号：US09703303
申请日：2000-10-31
IPC分类号： B01L302
CPC分类号： B01L3/0268 , B01J2219/00378 , B01J2219/00529 , B01J2219/00608 , B01J2219/00662 , B01J2219/00689 , B01J2219/00722 , B01L2200/14 , B01L2400/0439 , B01L2400/0442 , C40B40/06 , C40B60/14 , Y10T436/2575
摘要： A method, apparatus and computer program product, for fabricating an array of biopolymers on a substrate. The method uses a biopolymer or biomonomer fluid and a drop dispenser having a chamber into which the fluid is loaded and an orifice communicating with the chamber from which the fluid is dispensed. The method includes, when the chamber is loaded, applying a prime pressure to the fluid which varies over a range sufficient to move fluid within the drop dispenser but insufficient to cause fluid to be dispensed from the orifice. Drops are dispensed from the dispenser to the substrate so as to form the array.
摘要翻译： 一种用于在衬底上制造生物聚合物阵列的方法，装置和计算机程序产品。 该方法使用生物聚合物或生物共聚物流体和具有腔室的液滴分配器，流体被加载到该腔室中，以及与腔室连通的孔口，流体从该腔室分配。 该方法包括，当腔室被装载时，向在液滴分配器内足以移动流体的范围内变化的流体施加主压力，但不足以使流体从孔口分配。 从分配器将液滴分配到基底以形成阵列。
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公开(公告)号：US06613893B1
公开(公告)日：2003-09-02
申请号：US09629500
申请日：2000-07-31
申请人： Peter G. Webb
发明人： Peter G. Webb
IPC分类号： C07H2102
CPC分类号： B01J19/0046 , B01J2219/00378 , B01J2219/00475 , B01J2219/00527 , B01J2219/0054 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00626 , B01J2219/00637 , B01J2219/00659 , B01J2219/00689 , B01J2219/00695 , B01J2219/00722 , B01J2219/00725 , C40B40/06 , C40B40/10 , C40B60/14 , C40B70/00
摘要： A method, apparatus, and computer program, for fabricating multiple arrays arranged successively in a first direction on a substrate and each having multiple feature sets arranged successively in the first direction within the array. The method uses a head system having multiple successive sets of dispensers. In the method, the head system is advanced in the first direction over the substrate while dispensing drop sets for each array from dispenser sets so as to form the arrays. In one aspect, drop sets are dispensed in an order the reverse of that from which the dispenser sets pass over a given location on the substrate as the head system advances in the first direction. In this case, each dispenser set deposits a drop set at a distance ahead of a drop set deposited by a preceding dispenser set which is less than the distance to the successive drop dispenser set which deposits the next drop set.
摘要翻译： 一种方法，装置和计算机程序，用于制造在基板上沿第一方向连续布置的多个阵列，并且每个具有在阵列内的第一方向上连续布置的多个特征集。 该方法使用具有多个连续的分配器组的头系统。 在该方法中，头系统在衬底上沿第一方向前进，同时从分配器组分配每个阵列的滴组，以形成阵列。 在一个方面，当头系统沿着第一方向前进时，滴套以与分配器组在基板上的给定位置通过的顺序相反地分配。 在这种情况下，每个分配器装置将一个设定在距先前分配器组所沉积的液滴组件前面的距离设定的液滴小于与沉积下一个液滴组的连续液滴分配器组的距离。
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公开(公告)号：US06323043B1
公开(公告)日：2001-11-27
申请号：US09302922
申请日：1999-04-30
IPC分类号： G01N33543
CPC分类号： B01J19/0046 , B01J2219/00378 , B01J2219/00527 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00619 , B01J2219/00626 , B01J2219/00637 , B01J2219/00659 , B01J2219/00722 , B01J2219/00725 , C40B40/06 , C40B40/10 , C40B60/14 , Y10T436/2575
摘要： A method and apparatus for fabricating an array of biopolymers on a substrate using a biopolymer or biomonomer fluid, and using a dispensing head. The head has a reservoir chamber and at least one jet which can dispense droplets onto a substrate. The jet includes a capillary delivery chamber communicating with the reservoir chamber, which delivery chamber has an orifice. The jet further includes an ejector which, when activated, causes a droplet to be ejected from the orifice. The method includes loading the head by positioning the head with the orifice adjacent and facing a biomonomer or biopolymer fluid, and providing a load pressure to the reservoir chamber. The load pressure is sufficiently negative such that the fluid is drawn into the reservoir chamber through the orifice and delivery chamber, while simultaneously being insufficient to result in ambient atmosphere entering the delivery chamber through the orifice once the head has been loaded and no further fluid is facing and adjacent the orifice. The head is positioned with the orifice facing the substrate and multiple droplets are dispensed from the head orifice so as to form an array of droplets on the substrate.
摘要翻译： 一种使用生物聚合物或生物共聚物流体以及使用分配头在衬底上制造生物聚合物阵列的方法和装置。 头部具有储存室和至少一个可将液滴分配到基底上的射流。 喷射器包括与储存室连通的毛细管输送室，该输送室具有孔口。 射流还包括喷射器，其在被激活时使得液滴从孔口喷出。 该方法包括通过将头部与孔相邻并且面向生物体或生物聚合物流体的位置来装载头部，并向贮存室提供负载压力。 负载压力足够负，使得流体通过孔口和输送室被吸入储存室，同时不足以在头部已经被加载并且没有另外的流体是通过孔口导致环境大气通过孔口进入输送室 面对并邻近孔口。 头部定位成孔口面向衬底，并且从头部孔口分配多个液滴，以便在衬底上形成液滴阵列。
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公开(公告)号：US20100332953A1
公开(公告)日：2010-12-30
申请号：US12492487
申请日：2009-06-26
申请人： Peter G. Webb , Jayati Ghosh , Bo Curry
发明人： Peter G. Webb , Jayati Ghosh , Bo Curry
IPC分类号： H03M13/09
CPC分类号： G06F11/3055 , G06F11/0736 , G06F11/0748 , G06F11/076 , G06F11/3013 , G06F11/3058
摘要： Methods, systems and computer readable media for controlling an instrument in communication with a host computer are provided. Operations of an instrument that must be completed on schedule are controlled via an embedded controller embedded in the instrument. A complete status packet is sent to a host computer from the embedded controller Periodically, the embedded controller repeats the sending of a complete status packet to the host computer, wherein status values in the complete status packet are updated with each iteration of sending a complete status packet.
摘要翻译： 提供了用于控制与主计算机通信的仪器的方法，系统和计算机可读介质。 必须按计划完成的仪器的操作通过嵌入仪器中的嵌入式控制器进行控制。 完整的状态包从嵌入式控制器发送到主计算机周期性地，嵌入式控制器重复向主计算机发送完整的状态包，其中在完成状态包中的状态值被更新，每次迭代发送完整状态 包。
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15.发明申请Systems and methods for sub-genomic region specific comparative genome hybridization probe selection 审中-公开亚基因组区域特异性比较基因组杂交探针选择的系统和方法公开(公告)号：US20080243396A1
公开(公告)日：2008-10-02
申请号：US11729505
申请日：2007-03-28
申请人： Peter G. Webb , Jing Gao , B. Shane Giles , Sandra Tang
发明人： Peter G. Webb , Jing Gao , B. Shane Giles , Sandra Tang
CPC分类号： G16B25/00 , C12Q1/6841 , G16B20/00
摘要： Systems and methods for using the same to select one or more comparative genome hybridization (CGH) probes specific for a sub-genomic region of interest are provided. Also provided are computer program products for executing the subject methods.
摘要翻译： 提供了使用该技术的系统和方法来选择对感兴趣的亚基因组区域特异性的一个或多个比较基因组杂交（CGH）探针。 还提供了用于执行主题方法的计算机程序产品。
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16.发明授权公开(公告)号：US07399844B2
公开(公告)日：2008-07-15
申请号：US09836012
申请日：2001-04-17
申请人： Jeffrey R. Sampson , Joel Myerson , Anna M. Tsalenko , Nicholas M. Sampas , Peter G. Webb , Zohar H. Yakhini
发明人： Jeffrey R. Sampson , Joel Myerson , Anna M. Tsalenko , Nicholas M. Sampas , Peter G. Webb , Zohar H. Yakhini
CPC分类号： C12Q1/6872 , B01J2219/00452 , B01J2219/00454 , B01J2219/00497 , B01J2219/00527 , B01J2219/00581 , B01J2219/00585 , B01J2219/00596 , B01J2219/00599 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00626 , B01J2219/00637 , B01J2219/00641 , B01J2219/00659 , B01J2219/00707 , B01J2219/00722 , C12Q1/6874 , C12Q2600/156 , C40B40/00 , C12Q2537/143 , C12Q2533/101 , C12Q2533/107
摘要： Methods and reagents are disclosed which provide for more sensitive, more accurate and higher through-put analyses of target nucleic acid sequences. The methods and reagents of the present invention may be generically applied to generally any target nucleic acid sequence and do not require a priori information about the presence, location or identity of mutations in the target nucleic acid sequence. The reagents of the invention are mixtures of oligonucleotide precursors having a high level of coverage and mass number complexity, and also having tags analyzable by mass spectrometry which are covalently linked to the precursors through cleavable bonds. A method is also disclosed for analyzing a target nucleic acid sequence employing the mixtures of oligonucleotide precursors having tags analyzable by mass spectrometry covalently linked to the oligonucleotide precursors through cleavable bonds, and chemical or enzymatic assays to alter the mass of the oligonucleotide precursors prior to mass spectral analysis. The enzymatic assay may be a polymerase extension assay or a ligation-based assay. The kits for carrying out the methods of the invention are also disclosed.
摘要翻译： 公开了提供对靶核酸序列的更灵敏，更准确和更高的通过分析的方法和试剂。 本发明的方法和试剂通常可以一般地应用于任何靶核酸序列，并且不需要关于靶核酸序列中突变的存在，位置或同一性的先验信息。 本发明的试剂是具有高水平的覆盖率和质量数复杂性的寡核苷酸前体的混合物，并且还具有可通过质谱法分析的标签，其通过可切割键与前体共价连接。 还公开了一种用于分析靶核酸序列的方法，该寡核苷酸前体具有通过可通过可切割键与寡核苷酸前体共价连接的质谱分析的标签的寡核苷酸前体的混合物，以及在质量之前改变寡核苷酸前体的质量的化学或酶学测定 光谱分析。 酶测定可以是聚合酶延伸测定或基于连接的测定。 还公开了用于实施本发明的方法的试剂盒。
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公开(公告)号：US07282332B2
公开(公告)日：2007-10-16
申请号：US10932886
申请日：2004-09-01
IPC分类号： C12Q1/68 , G01N33/543
CPC分类号： B01J19/0046 , B01J2219/00378 , B01J2219/00527 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00619 , B01J2219/00626 , B01J2219/00637 , B01J2219/00659 , B01J2219/00722 , B01J2219/00725 , C40B40/06 , C40B40/10 , C40B60/14 , Y10T436/2575
摘要： A method and apparatus for fabricating an array of biopolymers on a substrate using a biopolymer or biomonomer fluid, and using a dispensing head. The head has a reservoir chamber and at least one jet which can dispense droplets onto a substrate. The jet includes a capillary delivery chamber communicating with the reservoir chamber, which delivery chamber has an orifice. The jet further includes an ejector which, when activated, causes a droplet to be ejected from the orifice. The method includes loading the head by positioning the head with the orifice adjacent and facing a biomonomer or biopolymer fluid, and providing a load pressure to the reservoir chamber. The load pressure is sufficiently negative such that the fluid is drawn into the reservoir chamber through the orifice and delivery chamber, while simultaneously being insufficient to result in ambient atmosphere entering the delivery chamber through the orifice once the head has been loaded and no further fluid is facing and adjacent the orifice. The head is positioned with the orifice facing the substrate and multiple droplets are dispensed from the head orifice so as to form an array of droplets on the substrate.
摘要翻译： 一种使用生物聚合物或生物共聚物流体以及使用分配头在衬底上制造生物聚合物阵列的方法和装置。 头部具有储存室和至少一个可将液滴分配到基底上的射流。 喷射器包括与储存室连通的毛细管输送室，该输送室具有孔口。 射流还包括喷射器，其在被激活时使得液滴从孔口喷出。 该方法包括通过将头部与孔相邻并且面向生物体或生物聚合物流体的位置来装载头部，并向贮存室提供负载压力。 负载压力足够负，使得流体通过孔口和输送室被吸入储存室，同时不足以在头部已经被加载并且没有另外的流体是通过孔口导致环境大气通过孔口进入输送室 面对并邻近孔口。 头部定位成孔口面向衬底，并且从头部孔口分配多个液滴，以便在衬底上形成液滴阵列。
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公开(公告)号：US07141368B2
公开(公告)日：2006-11-28
申请号：US10066518
申请日：2002-01-30
CPC分类号： B82Y30/00 , B01J19/0046 , B01J2219/00378 , B01J2219/00497 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00637 , B01J2219/00659 , B01J2219/00662 , B01J2219/00675 , B01J2219/00677 , B01J2219/00689 , B01J2219/00693 , B01J2219/00707 , B01J2219/00722 , B01J2219/00725 , B01J2219/00731 , B01L3/0241 , C40B40/06 , C40B40/10 , C40B40/12 , C40B50/14 , C40B60/14 , G01N35/109 , G01N2035/1041
摘要： A method, apparatus, and computer program products for fabricating multiple chemical arrays on a substrate, each array having multiple rows of feature locations with arrays of different sets being arranged in a sideways orientation with respect to the rows. The method includes dispensing drops from a drop dispensing head onto the substrate while maintaining a gap between the head and substrate and moving them relative to one another along a path so as to fabricate the arrays. The path for the relative moving includes moving the head in a direction along the rows of a first array set then moving the head in an opposite direction along the rows of a second array set. This pattern is repeated with the second array set of an earlier cycle being the first array set of a later cycle.
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19.发明授权Methods designing multiple mRNA transcript nucleic acid probe sequences for use in nucleic acid arrays 失效设计用于核酸阵列的多个mRNA转录核酸探针序列的方法公开(公告)号：US07029854B2
公开(公告)日：2006-04-18
申请号：US10303151
申请日：2002-11-22
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6876 , G06F19/22
摘要： Methods of identifying a sequence of a nucleic acid that is suitable for use as a surface immobilized probe for two or more mRNA transcripts encoded by the same gene are provided. In practicing the subject methods, a consensus region for the two or more transcripts is first identified, and this identified consensus region is then employed to identify the suitable nucleic acid sequence, e.g., by using a probe design protocol. The subject invention also includes algorithms for performing the subject methods recorded on a computer readable medium, as well as computational analysis systems that include the same. Also provided are nucleic acid arrays produced with probes having sequences identified by the subject methods, as well as methods for using the same.
摘要翻译： 提供了鉴定适合用作由相同基因编码的两个或更多个mRNA转录物的表面固定化探针的核酸序列的方法。 在实施本发明方法时，首先鉴定两个或多个转录物的共有区域，然后使用该鉴定的共有区域来鉴定合适的核酸序列，例如通过使用探针设计方案。 本发明还包括用于执行记录在计算机可读介质上的主题方法的算法，以及包括其的计算分析系统。 还提供了由具有由本发明方法鉴定的序列的探针产生的核酸阵列，以及使用该序列的方法。
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公开(公告)号：US06251588B1
公开(公告)日：2001-06-26
申请号：US09021701
申请日：1998-02-10
IPC分类号： C12Q168
摘要： Methods are disclosed for predicting the potential of an oligonucleotide to hybridize to a target nucleotide sequence. A predetermined number of unique oligonucleotides is identified. The unique oligonucleotides are chosen to sample the entire length of a nucleotide sequence that is hybridizable with the target nucleotide sequence. At least one parameter that is independently predictive of the ability of each of the oligonucleotides of the set to hybridize to the target nucleotide sequence is determined and evaluated for each of the above oligonucleotides. A subset of oligonucleotides within the predetermined number of unique oligonucleotides is identified based on the evaluation of the parameter. Oligonucleotides in the subset are identified that are clustered along a region of the nucleotide sequence that is hybridizable to the target nucleotide sequence. The method may be carried out with the aid of a computer.
摘要翻译： 公开了用于预测寡核苷酸与靶核苷酸序列杂交的潜力的方法。 鉴定出预定数量的唯一寡核苷酸。 选择独特的寡核苷酸来对可与靶核苷酸序列杂交的核苷酸序列的整个长度进行采样。 确定并评估每种上述寡核苷酸的至少一个独立地预测该组寡核苷酸与靶核苷酸序列杂交的能力的参数。 基于参数的评估来鉴定预定数量的唯一寡核苷酸内的寡核苷酸的子集。 鉴定了亚群中的寡核苷酸，其沿着与靶核苷酸序列可杂交的核苷酸序列的区域聚集。 该方法可以借助于计算机进行。
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