公开(公告)号：US20060099711A1

公开(公告)日：2006-05-11

申请号：US11267005

申请日：2005-11-04

申请人： Chang-eun Yoo ,  Joon-ho Kim ,  Kyu-youn Hwang ,  Hun-joo Lee ,  Hee-kyun Lim ,  Soo-min Ma ,  Nam Huh ,  Soo-suk Lee

发明人： Chang-eun Yoo ,  Joon-ho Kim ,  Kyu-youn Hwang ,  Hun-joo Lee ,  Hee-kyun Lim ,  Soo-min Ma ,  Nam Huh ,  Soo-suk Lee

IPC分类号： G01N1/00

CPC分类号： C12Q1/6806 ,  Y10T436/25 ,  C12Q2523/308

摘要： Provided is a lysis method for cells or viruses, including: immobilizing a metal-ligand complex on a solid support; and mixing the complex immobilized on the support with a cell or virus solution. According to the lysis method, by immobilizing a chemical on a solid support to perform cell lysis, the dilution problem according to the addition of a cell lysis solution can be resolved and a separate process of removing the chemical is not required so as to reduce the steps upon LOC implementation. In addition, since a variety of solid supports, such as chips, beads, nanoparticles etc. can be used, cell lysis apparatuses of various forms can be fabricated.

摘要翻译： 提供了一种细胞或病毒的裂解方法，包括：将固定支持物上的金属 - 配体复合物固定; 并将固定在载体上的复合物与细胞或病毒溶液混合。 根据裂解方法，通过将化学物质固定在固体支持体上以进行细胞裂解，可以解决根据细胞溶解溶液的添加的稀释问题，并且不需要单独的除去化学品的方法，以减少 LOC实施步骤。 此外，由于可以使用各种固体支持体，例如芯片，珠粒，纳米粒子等，因此可以制造各种形式的细胞裂解装置。

公开(公告)号：US07807360B2

公开(公告)日：2010-10-05

申请号：US11620961

申请日：2007-01-08

申请人： Young-rok Kim ,  Jun-hong Min ,  In-ho Lee ,  Young-sun Lee ,  Chang-eun Yoo ,  Ki-woong Han

发明人： Young-rok Kim ,  Jun-hong Min ,  In-ho Lee ,  Young-sun Lee ,  Chang-eun Yoo ,  Ki-woong Han

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6806 ,  B01L3/5027 ,  B01L3/5088 ,  C12Q1/686 ,  C12Q2565/629 ,  C12Q2537/149 ,  C12Q2527/125

摘要： A method of sequentially performing concentration and amplification of nucleic acid in a single micro chamber includes: introducing a nucleic acid-containing sample and a solution including a kosmotropic salt to a micro chamber having a hydrophilic interior surface to concentrate the nucleic acid by binding the nucleic acid on the interior surface of the micro chamber; and performing a polymerase chain reaction (PCR) by adding a PCR mixture to the chamber. Since the nucleic acid is reversibly bound to the interior surface of the micro chamber, PCR yield is higher compared with a surface of aluminum oxide in which irreversible binding occurs. In addition, all processes are sequentially performed in a single micro chamber so that the number of samples, consumables, time, and labor for treatment and analysis can be reduced, detection sensitivity can be improved, and risk of sample cross contamination significantly reduced without sample loss by eliminating transporting of the sample. A complete automated system for concentration and amplification of nucleic acid is thus readily provided.

摘要翻译： 在单个微室中依次进行核酸的浓缩和扩增的方法包括：将含核酸的样品和包含可染色盐的溶液引入具有亲水性内表面的微室中，以通过结合核酸来浓缩核酸 酸在微室的内表面上; 并通过向该室加入PCR混合物进行聚合酶链式反应（PCR）。 由于核酸可逆地结合到微室的内表面，与其中发生不可逆结合的氧化铝的表面相比，PCR产率更高。 此外，所有过程都在单个微室中顺序进行，以便可以减少样品数量，消耗品，时间和处理和分析的劳动力，可以提高检测灵敏度，并且样品交叉污染的风险在没有样品时显着降低 通过消除样品的运输而损失。 因此容易提供用于浓缩和扩增核酸的完整的自动化系统。

公开(公告)号：US07648838B2

公开(公告)日：2010-01-19

申请号：US11267005

申请日：2005-11-04

申请人： Chang-eun Yoo ,  Joon-ho Kim ,  Kyu-youn Hwang ,  Hun-joo Lee ,  Hee-kyun Lim ,  Soo-min Ma ,  Nam Huh ,  Soo-suk Lee

发明人： Chang-eun Yoo ,  Joon-ho Kim ,  Kyu-youn Hwang ,  Hun-joo Lee ,  Hee-kyun Lim ,  Soo-min Ma ,  Nam Huh ,  Soo-suk Lee

IPC分类号： G01N33/48 ,  G01N33/20 ,  G01N1/00

CPC分类号： C12Q1/6806 ,  Y10T436/25 ,  C12Q2523/308

摘要： Provided is a lysis method for cells or viruses, including: immobilizing a metal-ligand complex on a solid support; and mixing the complex immobilized on the support with a cell or virus solution. According to the lysis method, by immobilizing a chemical on a solid support to perform cell lysis, the dilution problem according to the addition of a cell lysis solution can be resolved and a separate process of removing the chemical is not required so as to reduce the steps upon LOC implementation. In addition, since a variety of solid supports, such as chips, beads, nanoparticles etc. can be used, cell lysis apparatuses of various forms can be fabricated.

摘要翻译： 提供了一种细胞或病毒的裂解方法，包括：将固定支持物上的金属 - 配体复合物固定; 并将固定在载体上的复合物与细胞或病毒溶液混合。 根据裂解方法，通过将化学物质固定在固体支持体上以进行细胞裂解，可以解决根据细胞溶解溶液的添加的稀释问题，并且不需要单独的除去化学品的方法，以减少 LOC实施步骤。 此外，由于可以使用各种固体支持物，例如芯片，珠粒，纳米粒子等，因此可以制造各种形式的细胞裂解装置。

公开(公告)号：US20060154360A1

公开(公告)日：2006-07-13

申请号：US11295843

申请日：2005-12-06

申请人： Soo-suk Lee ,  Sung-ouk Jung ,  Chang-eun Yoo ,  Hun-joo Lee ,  Christopher Ko ,  Nam Huh

发明人： Soo-suk Lee ,  Sung-ouk Jung ,  Chang-eun Yoo ,  Hun-joo Lee ,  Christopher Ko ,  Nam Huh

IPC分类号： C12M1/34

CPC分类号： B01L7/00 ,  B01F13/0059 ,  B01F13/0222 ,  B01L3/5027 ,  B01L2200/0689 ,  B01L2300/0636 ,  B01L2300/14 ,  B01L2300/1822 ,  B01L2400/0487 ,  G01N2035/00544

摘要： Provided is a hybridization system for hybridizing a biochip including: a chamber device including at least a hybridization chamber including a support for a biochip and a first cover having a sample inlet; an agitation device including: two air channels connected to ends of the hybridization chamber; two valves disposed in the air channels; an integrated air channel to which the two air channels are connected; and an air pump disposed in the integrated air channel; and a washing and drying device including: a flow channel connected to one of the two air channels through a branched valve; a flow pump disposed in the flow channel; and a buffer inlet disposed opposite the flow channel.

摘要翻译： 提供了一种用于杂交生物芯片的杂交系统，包括：至少包括一个包括用于生物芯片的支撑物的杂交室的腔室装置和具有样品入口的第一盖子; 一种搅拌装置，包括：两个连接到杂交室端部的空气通道; 设置在空气通道中的两个阀; 两个空气通道连接的集成空气通道; 以及设置在所述集成空气通道中的空气泵; 以及洗涤干燥装置，包括：流路，其通过分支阀连接到所述两个空气通道中的一个; 设置在流路中的流量泵; 以及与流动通道相对设置的缓冲器入口。

公开(公告)号：US20060147960A1

公开(公告)日：2006-07-06

申请号：US11295844

申请日：2005-12-06

申请人： Sung-ouk Jung ,  Nam Huh ,  Soo-suk Lee ,  Chang-eun Yoo ,  Hun-joo Lee

发明人： Sung-ouk Jung ,  Nam Huh ,  Soo-suk Lee ,  Chang-eun Yoo ,  Hun-joo Lee

IPC分类号： C12Q1/68 ,  C12M1/34 ,  B01F13/02

CPC分类号： B01L3/502738 ,  B01F13/0059 ,  B01F13/0222 ,  B01L3/50273 ,  B01L7/52 ,  B01L2300/0636 ,  B01L2300/0822 ,  B01L2300/0877

摘要： Provided is an agitation device used to agitate a solution in a hybridization chamber, the agitation device including: the hybridization chamber; first and second air channels connected to ends of the hybridization chamber; a first valve disposed in the first air channel; a second valve disposed in the second air channel; an integrated air channel connecting the first and second air channels; and a pump disposed in the integrated air channel. The agitation device is suitable for effective diffusion of a sample when performing hybridization using a DNA chip. Therefore, a probe can be effectively hybridized with a target material.

公开(公告)号：US20060134675A1

公开(公告)日：2006-06-22

申请号：US11297783

申请日：2005-12-07

申请人： Chang-eun Yoo ,  Joon-ho Kim ,  Kyu-youn Hwang ,  Hun-joo Lee ,  Hee-kyun Lim ,  Jun-hong Min

发明人： Chang-eun Yoo ,  Joon-ho Kim ,  Kyu-youn Hwang ,  Hun-joo Lee ,  Hee-kyun Lim ,  Jun-hong Min

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C07H21/04

摘要： Provided is a method of isolating and purifying nucleic acids using an immobilized hydrogel or polyethylene glycol (PEG)-hydrogel copolymer. The method includes: immobilizing a functional group-containing hydrogel or PEG-hydrogel copolymer on a substrate; adding a mixed sample solution containing a salt and nucleic acids to the hydrogel- or PEG-hydrogel copolymer-immobilized substrate to bind the nucleic acids to the hydrogel or the PEG-hydrogel copolymer; washing the nucleic acid-bound hydrogel or PEG-hydrogel copolymer; and eluting the nucleic acids from the hydrogel or the PEG-hydrogel copolymer using an elution solvent. Therefore, binding and elution of nucleic acids can be performed even with no addition of a separate chemical substance, and an effect on a subsequent process such as PCR can be minimized. Furthermore, the amount and intensity for binding nucleic acids can be adjusted according to PEG concentration, and the presence of a hydrogel compound on a substrate enables patterning.

摘要翻译： 提供了使用固定的水凝胶或聚乙二醇（PEG） - 水凝胶共聚物来分离和纯化核酸的方法。 该方法包括：将含官能团的水凝胶或PEG-水凝胶共聚物固定在基材上; 将含有盐和核酸的混合样品溶液加入到水凝胶或PEG-水凝胶共聚物固定的底物中，以将核酸与水凝胶或PEG-水凝胶共聚物结合; 洗涤核酸结合的水凝胶或PEG-水凝胶共聚物; 并使用洗脱溶剂从水凝胶或PEG-水凝胶共聚物中洗脱核酸。 因此，即使没有添加单独的化学物质也可以进行核酸的结合和洗脱，并且可以最小化对后续过程（例如PCR）的影响。 此外，可以根据PEG浓度调节结合核酸的量和强度，并且在基材上存在水凝胶化合物能够进行图案化。

公开(公告)号：US07914660B2

公开(公告)日：2011-03-29

申请号：US11303673

申请日：2005-12-16

申请人： Hun-joo Lee ,  Joon-ho Kim ,  Chang-eun Yoo ,  Kyu-youn Hwang ,  Hee-kyun Lim

发明人： Hun-joo Lee ,  Joon-ho Kim ,  Chang-eun Yoo ,  Kyu-youn Hwang ,  Hee-kyun Lim

IPC分类号： C25B1/10

CPC分类号： G05D21/02 ,  B01D61/422 ,  B01L3/50273 ,  B01L2200/14 ,  B01L2300/0867 ,  B01L2400/0415 ,  C02F1/4618 ,  C02F2103/02 ,  C02F2201/4611 ,  C02F2201/46115 ,  C02F2201/4618 ,  C02F2209/06 ,  Y02E60/366

摘要： Provided is a method of controlling the pH of a solution using electrolysis in a microfluidic device comprising an electrolysis device including an anode chamber, a cathode chamber, and a partition membrane between the anode chamber and the cathode chamber, wherein the anode chamber includes an inlet and an outlet through which an anode chamber solution enters and is discharged from the anode chamber, respectively, and an electrode, and the cathode chamber includes an inlet and an outlet through which a cathode chamber solution enters and is discharged from the cathode chamber, respectively, and an electrode. The method includes: flowing the anode chamber solution containing a compound having a lower standard oxidation potential than water into the anode chamber through the inlet of the anode chamber; flowing the cathode chamber solution containing a compound having a lower standard reduction potential than water into the cathode chamber through the inlet of the cathode chamber; applying voltage between the electrode in the anode chamber and the electrode in the cathode chamber to induce electrolysis in the anode chamber and the cathode chamber; and mixing equal volumes of the acidic anode-electrolyzed solution and the basic cathode-electrolyzed solution, wherein the volumes of the cathode chamber and the anode chamber are controlled in a predetermined ratio according to a target pH of a solution obtained by mixing the acidic anode-electrolyzed solution and the basic cathode-electrolyzed solution.

摘要翻译： 提供了一种在微流体装置中使用电解来控制溶液的pH的方法，该微流体装置包括在阳极室和阴极室之间包括阳极室，阴极室和分隔膜的电解装置，其中阳极室包括入口 以及阳极室溶液分别从阳极室进入和排出的出口以及电极，阴极室包括入口和出口，阴极室溶液分别从阴极室溶液进入并从阴极室排出 ，和电极。 该方法包括：将含有比水低的标准氧化电位的化合物的阳极室溶液通过阳极室的入口流入阳极室; 将含有比水低的标准还原电位的化合物的阴极室溶液通过阴极室的入口流入阴极室; 在阳极室中的电极和阴极室中的电极之间施加电压以在阳极室和阴极室中引起电解; 并混合等体积的酸性阳极电解溶液和碱性阴极电解溶液，其中阴极室和阳极室的体积根据通过将酸性阳极混合获得的溶液的目标pH控制在预定比例 电解液和碱性阴极电解溶液。

公开(公告)号：US07641858B2

公开(公告)日：2010-01-05

申请号：US11846794

申请日：2007-08-29

申请人： Young-rok Kim ,  Jun-hong Min ,  Kak Namkoong ,  Kwang-ho Cheong ,  Chang-eun Yoo ,  Ki-woong Han ,  Ki-eun Kim

发明人： Young-rok Kim ,  Jun-hong Min ,  Kak Namkoong ,  Kwang-ho Cheong ,  Chang-eun Yoo ,  Ki-woong Han ,  Ki-eun Kim

IPC分类号： B01L3/14 ,  B65B3/04

CPC分类号： B01L9/527 ,  B01L3/5021 ,  B01L3/5027 ,  B01L2200/027 ,  B01L2300/0832 ,  B01L2400/0409 ,  C12M23/16

摘要： An apparatus introducing a fluid using a centrifugal force includes an introduction member including a chip receiver and a fluid introduction reservoir, the chip receiver receiving a first part of a microfluidic chip, the first part including an inlet, the fluid introduction reservoir storing a fluid to be introduced to the microfluidic chip, the fluid introduction reservoir having an exit formed to correspond to the inlet of the microfluidic chip received in the chip receiver, and a support member supporting a second part of the microfluidic chip, wherein the microfluidic chip is disposed between the introduction member and the support member, the apparatus is rotatable in a state where the introduction member is closer to a center of rotation than the microfluidic chip, and the fluid is introducible from the fluid introduction reservoir through the inlet into the microfluidic chip due to a centrifugal force generated by rotation.

摘要翻译： 使用离心力引入流体的装置包括引入构件，其包括芯片接收器和流体引入储存器，所述芯片接收器接收微流体芯片的第一部分，所述第一部分包括入口，所述流体引入储存器将流体存储到 引入到微流体芯片中，流体引入储存器具有形成为对应于接收在芯片接收器中的微流体芯片的入口的出口，以及支撑微流体芯片的第二部分的支撑构件，其中微流体芯片设置在 引导构件和支撑构件，该装置可以在导入构件比微流体芯片更靠近旋转中心的状态下旋转，并且流体可以从流体引入储存器通过入口引入到微流体芯片中，这是由于 由旋转产生的离心力。

公开(公告)号：US07598064B2

公开(公告)日：2009-10-06

申请号：US12349273

申请日：2009-01-06

申请人： Hun-joo Lee ,  Joon-ho Kim ,  Chang-eun Yoo ,  Hee-kyun Lim ,  Kyu-youn Hwang ,  Soo-min Ma ,  Jun-hong Min

发明人： Hun-joo Lee ,  Joon-ho Kim ,  Chang-eun Yoo ,  Hee-kyun Lim ,  Kyu-youn Hwang ,  Soo-min Ma ,  Jun-hong Min

IPC分类号： C12N13/00 ,  C12N1/06 ,  C12M1/33 ,  C12M1/34 ,  C12Q1/70

CPC分类号： C12N1/066 ,  C12M47/06

摘要： Provided are a microfluidic device including an electrolysis device for cell lysis which includes an anode chamber, a cathode chamber and a separator, in which the separator is installed between the anode chamber and the cathode chamber, the anode chamber includes an inlet and an outlet for an anode chamber solution and an electrode, and the cathode chamber includes an inlet and an outlet for a cathode chamber solution and an electrode, and a method of electrochemically lysing cells using the same.

公开(公告)号：US20070235760A1

公开(公告)日：2007-10-11

申请号：US11733539

申请日：2007-04-10

申请人： Jeo-young Shim ,  Kyu-tae Yoo ,  Kyu-sang Lee ,  Won-seok Chung ,  Yeon-ja Cho ,  Chang-eun Yoo

发明人： Jeo-young Shim ,  Kyu-tae Yoo ,  Kyu-sang Lee ,  Won-seok Chung ,  Yeon-ja Cho ,  Chang-eun Yoo

IPC分类号： H01L31/00

CPC分类号： H01L29/78 ,  G01N27/4145

摘要： A field effect transistor for detecting an analyte having a thiol group includes a substrate, a source region and a drain region formed apart from each other on the substrate, the source region and the drain region being doped such that a polarity of the source and drain region is opposite to a polarity of the substrate, a channel region disposed between the source region and the drain region, an insulating layer formed of an electrically insulating material and disposed on the channel region, a gold layer disposed on the insulating layer and a reference electrode disposed apart from the gold layer.

摘要翻译： 用于检测具有硫醇基的分析物的场效应晶体管包括在衬底上彼此分开形成的衬底，源极区和漏极区，源极区和漏极区被掺杂，使得源极和漏极的极性 区域与基板的极性相反，设置在源极区域和漏极区域之间的沟道区域，由电绝缘材料形成并设置在沟道区域上的绝缘层，设置在绝缘层上的金层和参考 电极与金层分开设置。