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公开(公告)号：US20090298075A1
公开(公告)日：2009-12-03
申请号：US12413258
申请日：2009-03-27
申请人： Kevin Travers , Geoff Otto , Stephen Turner , Cheryl Heiner , Congcong Ma
发明人： Kevin Travers , Geoff Otto , Stephen Turner , Cheryl Heiner , Congcong Ma
IPC分类号： C12Q1/68
CPC分类号： G06F19/22 , C12Q1/6806 , C12Q1/6855 , C12Q1/6869 , C12Q1/689 , C12Q2525/307 , C12Q2525/301 , C12Q2525/155 , C12Q2531/125 , C12Q2535/122
摘要： Compositions and methods for nucleic acid sequencing include template constructs that comprise double stranded portions in a partially or completely contiguous constructs, to provide for redundant sequence determination through one or both of sequencing sense and antisense strands, and iteratively sequencing the entire construct multiple times. Additional sequence components are also optionally included within such template constructs. Methods are also provided for the use and preparation of these constructs as well as sequencing compositions for their application.
摘要翻译： 用于核酸测序的组合物和方法包括在部分或完全邻接的构建体中包含双链部分的模板构建体，以通过测序有义链和反义链中的一个或两个进行冗余序列测定，并对整个构建体进行多次迭代测序。 额外的序列成分也可选地包括在这样的模板构建体内。 还提供了这些构建体的使用和制备方法以及其应用的测序组合物。
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公开(公告)号：US07593819B2
公开(公告)日：2009-09-22
申请号：US10193776
申请日：2002-07-11
CPC分类号： G01N27/44726
摘要： The present teachings relate, among other things, to polynucleotide sequencing, fragment analysis and sample/lane tracking, and to polynucleotide sequencers and analyzers that employ optical detection techniques. Embodiments of the present teachings are described which include, for example, the addition of a calibration standard to a sequencing reaction. Information such as peak spacing and peak shape can he extracted from the standard.
摘要翻译： 本教导涉及多核苷酸测序，片段分析和样品/泳道跟踪，以及使用光学检测技术的多核苷酸测序仪和分析仪。 描述了本教导的实施例，其包括例如向测序反应添加校准标准。 可以从标准中提取峰间距和峰形等信息。
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公开(公告)号：US09175338B2
公开(公告)日：2015-11-03
申请号：US12945767
申请日：2010-11-12
申请人： Benjamin Flusberg , Jonas Korlach , Jeffrey Wegener , Tyson A. Clark , Igor Vilfan , Andrey Kislyuk , Stephen Turner , Jon Sorenson , Kevin Travers , Cheryl Heiner , Austin B. Tomaney , Patrick Marks , Jessica Lee , Lei Jia , Dale Webster , John Lyle , Jeremiah Hanes , Joseph Puglisi
发明人： Benjamin Flusberg , Jonas Korlach , Jeffrey Wegener , Tyson A. Clark , Igor Vilfan , Andrey Kislyuk , Stephen Turner , Jon Sorenson , Kevin Travers , Cheryl Heiner , Austin B. Tomaney , Patrick Marks , Jessica Lee , Lei Jia , Dale Webster , John Lyle , Jeremiah Hanes , Joseph Puglisi
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C12Q1/6837 , C12Q1/6858 , C12Q1/6874 , C12Q1/689 , C12Q2600/154 , C12Q2537/164 , C12Q2527/113 , C12Q2521/101 , C12Q2525/307 , C12Q2525/117
摘要： Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.
摘要翻译： 提供了方法，组合物和系统用于表征修饰的核酸。 在某些优选实施方案中，提供单分子测序方法用于鉴定核酸序列内的修饰的核苷酸。 通过本文提供的方法可检测的修饰物包括化学修饰的碱基，酶修饰的碱基，脱碱基位点，非天然碱基，二级结构和与模板核酸结合的试剂。
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公开(公告)号：US20120003699A1
公开(公告)日：2012-01-05
申请号：US13171766
申请日：2011-06-29
申请人： Cheryl Heiner , Stephen Turner , Kevin Travers
发明人： Cheryl Heiner , Stephen Turner , Kevin Travers
CPC分类号： C12Q1/6806 , C12Q1/6869 , C12Q2563/179 , C12Q2563/155 , C12Q2525/307
摘要： Methods of separating tagged nucleic acid fragments from populations of fragments are provided. The fragments are produced from a population of tagged nucleic acids. In some cases, the population of tagged nucleic acids comprises a population of clonal nucleic acids.
摘要翻译： 提供了从片段群体分离标记的核酸片段的方法。 片段由标记核酸群体产生。 在一些情况下，标记核酸的群体包括克隆核酸群体。
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公开(公告)号：US20090105094A1
公开(公告)日：2009-04-23
申请号：US12286119
申请日：2008-09-26
申请人： Cheryl Heiner , Stephen Turner , Kevin Travers
发明人： Cheryl Heiner , Stephen Turner , Kevin Travers
IPC分类号： C40B50/06
CPC分类号： C12Q1/6806 , C12Q1/6869 , C12Q2563/179 , C12Q2563/155 , C12Q2525/307
摘要： Methods of preparing nucleic acid templates and providing the nucleic acid templates to low copy number reaction volumes are provided. Related compositions of nucleic acid templates are also provided.
摘要翻译： 提供了制备核酸模板并将核酸模板提供给低拷贝数反应体积的方法。 还提供了核酸模板的相关组合物。
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公开(公告)号：US20120289408A1
公开(公告)日：2012-11-15
申请号：US13403789
申请日：2012-02-23
申请人： Kevin Travers , Geoff Otto , Stephen Turner , Cheryl Heiner , Congcong Ma
发明人： Kevin Travers , Geoff Otto , Stephen Turner , Cheryl Heiner , Congcong Ma
CPC分类号： G06F19/22 , C12Q1/6806 , C12Q1/6855 , C12Q1/6869 , C12Q1/689 , C12Q2525/307 , C12Q2525/301 , C12Q2525/155 , C12Q2531/125 , C12Q2535/122
摘要： Compositions and methods for nucleic acid sequencing include template constructs that comprise double stranded portions in a partially or completely contiguous constructs, to provide for redundant sequence determination through one or both of sequencing sense and antisense strands, and iteratively sequencing the entire construct multiple times. Additional sequence components are also optionally included within such template constructs. Methods are also provided for the use and preparation of these constructs as well as sequencing compositions for their application.
摘要翻译： 用于核酸测序的组合物和方法包括在部分或完全邻接的构建体中包含双链部分的模板构建体，以通过测序有义链和反义链中的一个或两个进行冗余序列测定，并对整个构建体进行多次迭代测序。 额外的序列成分也可选地包括在这样的模板构建体内。 还提供了这些构建体的使用和制备方法以及其应用的测序组合物。
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公开(公告)号：US08236499B2
公开(公告)日：2012-08-07
申请号：US12383855
申请日：2009-03-27
申请人： Pranav Patel , Keith Bjornson , Kevin Travers , Cheryl Heiner
发明人： Pranav Patel , Keith Bjornson , Kevin Travers , Cheryl Heiner
摘要： Provided are methods and compositions for the production of linear single-stranded nucleic acids, which can be used as templates in high-throughput sequencing systems. Also provided are methods and compositions for the production of closed single-stranded nucleic acid loops, which can be used as templates in high-throughput sequencing systems.
摘要翻译： 提供用于生产线性单链核酸的方法和组合物，其可以在高通量测序系统中用作模板。 还提供了用于生产封闭的单链核酸环的方法和组合物，其可以在高通量测序系统中用作模板。
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公开(公告)号：US20090280538A1
公开(公告)日：2009-11-12
申请号：US12383855
申请日：2009-03-27
申请人： Pranav Patel , Keith Bjornson , Kevin Travers , Cheryl Heiner
发明人： Pranav Patel , Keith Bjornson , Kevin Travers , Cheryl Heiner
IPC分类号： C12P19/34
摘要： Provided are methods and compositions for the production of linear single-stranded nucleic acids, which can be used as templates in high-throughput sequencing systems. Also provided are methods and compositions for the production of closed single-stranded nucleic acid loops, which can be used as templates in high-throughput sequencing systems.
摘要翻译： 提供用于生产线性单链核酸的方法和组合物，其可以在高通量测序系统中用作模板。 还提供了用于生产封闭的单链核酸环的方法和组合物，其可以在高通量测序系统中用作模板。
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公开(公告)号：US08535886B2
公开(公告)日：2013-09-17
申请号：US13494729
申请日：2012-06-12
申请人： Pranav Patel , Keith Bjornson , Kevin Travers , Cheryl Heiner
发明人： Pranav Patel , Keith Bjornson , Kevin Travers , Cheryl Heiner
摘要： Provided are methods and compositions for the production of linear single-stranded nucleic acids, which can be used as templates in high-throughput sequencing systems. Also provided are methods and compositions for the production of closed single-stranded nucleic acid loops, which can be used as templates in high-throughput sequencing systems.
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公开(公告)号：US08455193B2
公开(公告)日：2013-06-04
申请号：US13403789
申请日：2012-02-23
申请人： Kevin Travers , Geoff Otto , Stephen Turner , Cheryl Heiner , Congcong Ma
发明人： Kevin Travers , Geoff Otto , Stephen Turner , Cheryl Heiner , Congcong Ma
CPC分类号： G06F19/22 , C12Q1/6806 , C12Q1/6855 , C12Q1/6869 , C12Q1/689 , C12Q2525/307 , C12Q2525/301 , C12Q2525/155 , C12Q2531/125 , C12Q2535/122
摘要： Compositions and methods for nucleic acid sequencing include template constructs that comprise double stranded portions in a partially or completely contiguous constructs, to provide for redundant sequence determination through one or both of sequencing sense and antisense strands, and iteratively sequencing the entire construct multiple times. Additional sequence components are also optionally included within such template constructs. Methods are also provided for the use and preparation of these constructs as well as sequencing compositions for their application.
摘要翻译： 用于核酸测序的组合物和方法包括在部分或完全邻接的构建体中包含双链部分的模板构建体，以通过测序有义链和反义链中的一个或两个进行冗余序列测定，并对整个构建体进行多次迭代测序。 额外的序列成分也可选地包括在这样的模板构建体内。 还提供了这些构建体的使用和制备方法以及其应用的测序组合物。
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