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11.发明授权Intracellular metabolic flux analysis method using substrate labeled with isotope 失效使用同位素标记的底物的细胞内代谢通量分析方法公开(公告)号：US08510054B2
公开(公告)日：2013-08-13
申请号：US11048923
申请日：2005-02-03
CPC分类号： G01N33/5091 , C12Q1/00 , G06F19/12
摘要： A method for intracellular metabolic flux analysis comprising (a) culturing cells in a medium not containing any isotope-labeled substrate to a target phase of the metabolic flux analysis, (b) adding an isotope-labeled substrate to the medium, and continuing culture and collecting samples from the medium in time course, (c) measuring isotope distribution in an intracellular metabolite contained in the samples collected in time course, (d) performing a regression analysis for measured data and calculating an isotope distribution ratio in a steady state, and (e) analyzing a metabolic flux of the cultured cells by using the calculated isotope distribution ratio.
摘要翻译： 一种用于细胞内代谢通量分析的方法，包括（a）在不含任何同位素标记的底物的培养基中将细胞培养至代谢通量分析的目标阶段，（b）将同位素标记的底物加入到培养基中， 在时间过程中从培养基中收集样品，（c）测量在时间过程中收集的样品中所含的细胞内代谢物中的同位素分布，（d）对稳定状态进行测量数据和计算同位素分布比的回归分析，以及 （e）使用计算出的同位素分布比分析培养细胞的代谢通量。
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公开(公告)号：US08076111B2
公开(公告)日：2011-12-13
申请号：US12579594
申请日：2009-10-15
IPC分类号： C12P7/46 , C12P1/04 , C12P21/06 , C12P19/34 , C12N1/20 , C12N9/00 , C07H21/02 , C07H21/04 , C07K1/00
CPC分类号： C12P7/46
摘要： An organic acid is produced by allowing a bacterium which has an ability to produce an organic acid and has been modified so that expression of yidE gene is enhanced, or a product obtained by processing the bacterium, to act on an organic raw material in a reaction mixture containing carbonate ions, bicarbonate ions, or carbon dioxide gas to produce the organic acid, and collecting the organic acid.
摘要翻译： 有机酸是通过使具有生产有机酸的能力的细菌进行改性的，以使yidE基因的表达增强，或通过加工细菌获得的产物作用于反应中的有机原料 含有碳酸根离子，碳酸氢根离子或二氧化碳气体的混合物以产生有机酸，并收集有机酸。
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公开(公告)号：US08030036B2
公开(公告)日：2011-10-04
申请号：US11877726
申请日：2007-10-24
申请人： Stephen Van Dien , Shintaro Iwatani , Yoshihiro Usuda , Kazuhiko Matsui , Yuta Nakai , Tomoko Suzuki , Mika Moriya , Yuichiro Tsuji , Takuji Ueda
发明人： Stephen Van Dien , Shintaro Iwatani , Yoshihiro Usuda , Kazuhiko Matsui , Yuta Nakai , Tomoko Suzuki , Mika Moriya , Yuichiro Tsuji , Takuji Ueda
CPC分类号： C12N9/0006 , C12P13/08
摘要： A bacterium belonging to the genus Escherichia which has an ability to produce L-lysine or L-threonine and which is modified so that a malic enzyme does not function normally in a cell, and a method for producing L-lysine or L-threonine, comprising culturing the bacterium in a medium to produce and cause accumulation of L-lysine or L-threonine, and collecting the L-lysine or L-threonine from the medium.
摘要翻译： 属于埃希氏菌属的细菌，其具有产生L-赖氨酸或L-苏氨酸的能力，并且其被修饰以使得苹果酸在细胞中不起作用;以及L-赖氨酸或L-苏氨酸的制备方法， 包括在培养基中培养细菌以产生并引起L-赖氨酸或L-苏氨酸的积累，并从培养基中收集L-赖氨酸或L-苏氨酸。
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公开(公告)号：US20090239269A1
公开(公告)日：2009-09-24
申请号：US12420934
申请日：2009-04-09
CPC分类号： C12N9/88 , C12P13/08 , C12P13/227
摘要： A bacterium which belongs to the Enterobacteriaceae family and has an ability to produce an L-amino acid such as L-lysine, L-threonine and L-tryptophan and is modified to enhance glutamic acid decarboxylase activity is cultured in a medium to produce and accumulate the L-amino acid in the medium or cells of the bacterium. Then, the L-amino acid is collected from the medium or the cells.
摘要翻译： 属于肠杆菌科的具有生产L-赖氨酸，L-苏氨酸和L-色氨酸的L-氨基酸的能力并经过修饰以增强谷氨酸脱羧酶活性的细菌在培养基中培养以产生和积累 细菌的培养基或细胞中的L-氨基酸。 然后，从培养基或细胞收集L-氨基酸。
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公开(公告)号：US20090093029A1
公开(公告)日：2009-04-09
申请号：US12202484
申请日：2008-09-02
申请人： Yoshihiro Usuda , Kazuhiko Matsui
发明人： Yoshihiro Usuda , Kazuhiko Matsui
CPC分类号： C12P13/08 , C12P13/04 , C12P13/14 , C12P13/227
摘要： An L-amino acid is produced by culturing an Enterobacteriaceae which is able to produce an L-amino acid in a medium containing glycerol, especially crude glycerol, as the carbon source to produce and accumulate the L-amino acid in the culture, and collecting the L-amino acid from the culture.
摘要翻译： 通过培养能够在含有甘油，特别是粗甘油的培养基中产生L-氨基酸的肠杆菌科生产L-氨基酸作为碳源，以在培养物中产生和积累L-氨基酸，并收集 来自培养物的L-氨基酸。
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公开(公告)号：US07306933B2
公开(公告)日：2007-12-11
申请号：US11275437
申请日：2006-01-03
申请人： Stephen Van Dien , Shintaro Iwatani , Yoshihiro Usuda , Kazuhiko Matsui , Yuta Nakai , Tomoko Suzuki , Mika Moriya , Yuichiro Tsuji , Takuji Ueda
发明人： Stephen Van Dien , Shintaro Iwatani , Yoshihiro Usuda , Kazuhiko Matsui , Yuta Nakai , Tomoko Suzuki , Mika Moriya , Yuichiro Tsuji , Takuji Ueda
CPC分类号： C12N9/0006 , C12P13/08
摘要： A bacterium belonging to the genus Escherichia which has an ability to produce L-lysine or L-threonine and which is modified so that a malic enzyme does not function normally in a cell, and a method for producing L-lysine or L-threonine, comprising culturing the bacterium in a medium to produce and cause accumulation of L-lysine or L-threonine, and collecting the L-lysine or L-threonine from the medium.
摘要翻译： 属于埃希氏菌属的细菌，其具有产生L-赖氨酸或L-苏氨酸的能力，并且其被修饰以使得苹果酸在细胞中不起作用;以及L-赖氨酸或L-苏氨酸的制备方法， 包括在培养基中培养细菌以产生并引起L-赖氨酸或L-苏氨酸的积累，并从培养基中收集L-赖氨酸或L-苏氨酸。
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公开(公告)号：US20070038419A1
公开(公告)日：2007-02-15
申请号：US11501232
申请日：2006-08-09
IPC分类号： G06F17/10
CPC分类号： G16B5/00
摘要： A method for simulating a substance production process using cells based on a set of differential equations that represent intracellular metabolites and gene expression comprising including a specific growth rate of cells, expressed as a differential equation, in the set of differential equations; assigning values for parameters in the set of differential equations, wherein at least one of said parameters is represented as a growth rate factor; incorporating in the set of differential equations a formation rate for formation of a cell component, wherein said formation rate is represented as a growth rate factor; incorporating in the set of differential equations an inflow rate of a metabolite taken from outside of the cells and/or an outflow rate of a metabolite excreted out of the cells, wherein said inflow/outflow rates are represented as a growth rate factor; solving the set of differential equations; and generating data representative of the substance-production process.
摘要翻译： 一种基于代表细胞内代谢物和基因表达的微分方程组，使用细胞模拟物质生产过程的方法，其包括在微分方程组中包含表示为微分方程的细胞的比生长速率; 为所述微分方程组中的参数分配值，其中所述参数中的至少一个被表示为增长率因子; 在微分方程组中并入形成细胞成分的形成速率，其中所述形成速率表示为生长速率因子; 在微分方程组中引入从细胞外部获取的代谢物的流入速率和/或从细胞排出的代谢物的流出速率，其中所述流入/流出速率表示为生长速率因子; 求解微分方程组; 并产生代表物质生产过程的数据。
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18.发明申请NOVEL POLYNUCLEOTIDES ENCODING USEFUL POLYPEPTIDES IN CORYNEBACTERIUM GLUTAMICUM SSP. LACTOFERMENTUM 失效新型多核苷酸编码有用的多糖在玉米淀粉SSP中。 LACTOFERMENTUM公开(公告)号：US20060234356A1
公开(公告)日：2006-10-19
申请号：US11422963
申请日：2006-06-08
CPC分类号： C12N9/0016 , C07H21/04 , C07K14/34 , C12N9/1007 , C12N9/22 , C12P13/04 , C12P21/00 , C12P21/02
摘要： The present invention provides novel polypeptides and polynucleotides useful as biotechnological tools, specifically identified in a coryneform bacterium Corynebacterium glutamicum ssp. lactofermentum and methods of producing substances in organisms having enhanced or attenuated expression of these polypeptides and/or polynucleotides.
摘要翻译： 本发明提供了用作生物技术工具的新型多肽和多核苷酸，其特异性在棒状杆菌型谷氨酸棒杆菌（Corynebacterium glutamicum） 乳发酵和在这些多肽和/或多核苷酸具有增强或减弱的表达的生物体中产生物质的方法。
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公开(公告)号：US09644009B2
公开(公告)日：2017-05-09
申请号：US12055438
申请日：2008-03-26
IPC分类号： C12P13/04 , C07K14/245 , C12P13/08 , C12P13/22
CPC分类号： C07K14/245 , C12P13/04 , C12P13/08 , C12P13/22
摘要： An L-amino acid is produced by culturing a microorganism of the family Enterobacteriaceae which has the ability to produce an L-amino acid and which has been modified so as to increase the expression of the evgA gene, the gadE gene, and/or the ydeO gene. These genes encode a transcription factor involved in the EvgAS two-component system regulon. The culture takes place in a medium, and the L-amino acid is collected from the medium or cells.
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公开(公告)号：US08512987B2
公开(公告)日：2013-08-20
申请号：US12545966
申请日：2009-08-24
申请人： Yuri Nagai , Kazuyuki Hayashi , Takuji Ueda , Yoshihiro Usuda , Kazuhiko Matsui
发明人： Yuri Nagai , Kazuyuki Hayashi , Takuji Ueda , Yoshihiro Usuda , Kazuhiko Matsui
IPC分类号： C12P13/24
摘要： An L-amino acid is produced by culturing a microorganism belonging to the family Enterobacteriaceae having an L-amino acid-producing ability and modified so that glycerol dehydrogenase and dihydroxyacetone kinase activities are increased, in a medium containing glycerol as a carbon source to produce and accumulate an L-amino acid in the medium or cells, and collecting the L-amino acid from the medium or the cells.
摘要翻译： 通过在含有甘油作为碳源的培养基中培养属于具有L-氨基酸生产能力的肠杆菌科的微生物并进行修饰以使甘油脱氢酶和二羟基丙酮激酶活性增加的L-氨基酸产生， 在培养基或细胞中积累L-氨基酸，并从培养基或细胞中收集L-氨基酸。
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