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公开(公告)号：US08075882B2
公开(公告)日：2011-12-13
申请号：US12653090
申请日：2009-12-08
CPC分类号： C12N5/0068 , A61K2039/5154 , C12N2533/70
摘要： Adoptive immune cells obtained by a method including (a) obtaining mammalian antigen-presenting associated cells; (b) culturing the resulting cells from step (a) in a culture liquid contained in a culture vessel coated with a sugar chain-containing polymer; and (c) detaching the cells from step (b) by shaking the culture vessel without treating the cells with an enzyme and without using a cell detaching tool. A method for treating a malignant tumor, type I diabetes, an atopic allergic disease or an infection, by administering the adoptive immune cells to a patient. A pharmaceutical composition for treating a malignant tumor, type I diabetes, an atopic allergic disease or an infection, including the adoptive immune cells and a pharmaceutically acceptable carrier.
摘要翻译： 通过包括（a）获得哺乳动物抗原呈递相关细胞的方法获得的免疫免疫细胞; （b）从包含在含有糖链的聚合物的培养容器中的培养液中培养来自步骤（a）的所得细胞; 和（c）通过摇动培养容器从步骤（b）中分离细胞而不用酶处理细胞，而不使用细胞分离工具。 通过向患者施用过继免疫细胞来治疗恶性肿瘤，I型糖尿病，特应性过敏性疾病或感染的方法。 用于治疗恶性肿瘤，I型糖尿病，特应性过敏性疾病或感染的药物组合物，包括过继免疫细胞和药学上可接受的载体。
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公开(公告)号：US20110024911A1
公开(公告)日：2011-02-03
申请号：US12834807
申请日：2010-07-12
IPC分类号： H01L23/482 , H01L21/66
CPC分类号： G01R27/14 , G01R1/07307 , H01L22/32 , H01L24/06 , H01L24/45 , H01L24/48 , H01L24/49 , H01L2224/02166 , H01L2224/05553 , H01L2224/05554 , H01L2224/05624 , H01L2224/0603 , H01L2224/32225 , H01L2224/45144 , H01L2224/48091 , H01L2224/48108 , H01L2224/48227 , H01L2224/48624 , H01L2224/49 , H01L2224/73265 , H01L2924/01005 , H01L2924/01006 , H01L2924/01013 , H01L2924/01014 , H01L2924/01029 , H01L2924/01033 , H01L2924/01047 , H01L2924/0105 , H01L2924/01074 , H01L2924/01075 , H01L2924/01078 , H01L2924/01079 , H01L2924/01082 , H01L2924/014 , H01L2924/10253 , H01L2924/13091 , H01L2924/14 , H01L2924/15311 , H01L2924/181 , H01L2924/3011 , H01L2924/351 , H01L2924/00014 , H01L2924/00 , H01L2924/00012
摘要： An electrical characteristics test for a semiconductor integrated circuit using a Kelvin contact method can be conducted in a pre-process without obstructing the reduction in size of a semiconductor chip or without complicating the circuit design. A probe card in a testing apparatus includes probes for Kelvin contact, the probes for Kelvin contact including a coil probe and a POGO pin probe disposed inside the coil probe, and a probe for two-terminal measurement. Electrode pads formed in each chip area over a wafer are in a relation of A=B
摘要翻译： 使用开尔文接触方法的半导体集成电路的电特性测试可以在预处理中进行，而不会妨碍半导体芯片尺寸的减小或不使电路设计复杂化。 测试装置中的探针卡包括用于开尔文接触的探针，用于开尔文接触的探针包括线圈探针和设置在线圈探针内的POGO针探针，以及用于两端测量的探针。 考虑到用于开尔文接触的探针与其接触的电极焊盘之一的面积为B，另一个电极的面积为A = B <2A，则晶片上每个芯片区域中形成的电极焊盘的关系为A = B <2A 用于两端测量的探头接触的垫是A.
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公开(公告)号：US20100184162A1
公开(公告)日：2010-07-22
申请号：US12184637
申请日：2008-08-01
申请人： Akira Imaizumi , Larisa Gotlibovna Airikh , Vera Georgievna Doroshenko , Irina Sergeevna Tsyrenzhapova
发明人： Akira Imaizumi , Larisa Gotlibovna Airikh , Vera Georgievna Doroshenko , Irina Sergeevna Tsyrenzhapova
CPC分类号： C12N15/70 , C12N1/38 , C12P13/08 , C12P13/14 , C12P13/222 , C12P13/227
摘要： A method is provided for producing an L-amino acid by culturing a microorganism belonging to the Enterobacteriaceae family and having the ability to produce an L-amino acid, in a medium to produce and accumulate the L-amino acid in the medium. The microorganism has been modified by introduction of a DNA fragment which includes a pho regulon promoter and a structural gene encoding an L-amino acid biosynthetic enzyme, which is ligated downstream of the promoter so that the gene is expressed by the promoter, and so that the activity of the L-amino acid biosynthetic enzyme is increased by the expression of the gene by the promoter. In this way, the L-amino acid that is produced in the medium can be collected. Furthermore, the phosphorus concentration in the medium is such that the expression of the gene by the promoter is induced.
摘要翻译： 提供了通过在培养基中培养属于肠杆菌科的微生物并具有产生L-氨基酸能力的培养基来生产和积累L-氨基酸的方法来生产L-氨基酸的方法。 通过引入包含光调节启动子和编码L-氨基酸生物合成酶的结构基因的DNA片段修饰微生物，该结构基因连接在启动子的下游，使得该基因由启动子表达，并且使得 L-氨基酸生物合成酶的活性通过启动子的表达而增加。 以这种方式，可以收集在培养基中产生的L-氨基酸。 此外，培养基中的磷浓度使得促进基因的表达被诱导。
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公开(公告)号：US07655393B2
公开(公告)日：2010-02-02
申请号：US11579280
申请日：2004-05-11
IPC分类号： A01N1/02
CPC分类号： C12N5/0068 , A61K2039/5154 , C12N2533/70
摘要： The present invention provides a method for preparing adoptive immune cells comprising the following steps of a) obtaining mammalian antigen-presenting associated cells; b) culturing the resulting cells in a culture vessel coated with a sugar chain-containing polymer; and c) detaching the cells by shaking the culture vessel without treating the cells with enzyme and without using a cell detaching tool; adoptive immune cells obtained by this preparing method; a culture vessel coated with a sugar chain-containing polymer for use in this preparation method; a kit for preparing an adoptive immune cell vaccine; and a method and a medicine for treating a malignant tumor, type I diabetes, atopic allergic diseases or infections using the adoptive immune cells.
摘要翻译： 本发明提供一种制备过继免疫细胞的方法，包括以下步骤：a）获得哺乳动物抗原呈递相关细胞; b）将所得细胞培养在涂有含糖链的聚合物的培养容器中; 和c）通过摇动培养容器而不用酶处理细胞并且不使用细胞分离工具来分离细胞; 通过该制备方法获得的过继免疫细胞; 用该制备方法使用的含糖链聚合物的培养容器; 用于制备过继性免疫细胞疫苗的试剂盒; 以及使用过继免疫细胞治疗恶性肿瘤，I型糖尿病，特应性过敏性疾病或感染的方法和药物。
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5.发明申请公开(公告)号：US20110035156A1
公开(公告)日：2011-02-10
申请号：US12805564
申请日：2010-08-05
申请人： Akira Imaizumi , Toshihiko Ando , Takeshi Kimura , Yasushi Noguchi , Akira Gouchi , Hiroshi Yamamoto
发明人： Akira Imaizumi , Toshihiko Ando , Takeshi Kimura , Yasushi Noguchi , Akira Gouchi , Hiroshi Yamamoto
CPC分类号： G01N33/57446 , G01N33/6812 , G16B40/00
摘要： According to the method of evaluating gastric cancer of the present invention, amino acid concentration data on the concentration value of amino acid in blood collected from a subject to be evaluated is measured, and a gastric cancer state in the subject is evaluated based on the concentration value of at least one of Asn, Cys, His, Met, Orn, Phe, Trp, Pro, Lys, Leu, Glu, Arg, Ala, Thr, and Tyr contained in the measured amino acid concentration data of the subject.
摘要翻译： 根据本发明的胃癌的评价方法，测定从评价对象收集的血液中的氨基酸浓度的氨基酸浓度数据，并根据浓度来评价受试者的胃癌状态 受试者的测定的氨基酸浓度数据中包含的Asn，Cys，His，Met，Orn，Phe，Trp，Pro，Lys，Leu，Glu，Arg，Ala，Thr和Tyr中的至少一种的值。
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公开(公告)号：US20050106688A1
公开(公告)日：2005-05-19
申请号：US10862424
申请日：2004-06-08
摘要： An L-amino acid is produced by culturing a bacterium having an ability to produce an L-amino acid in a medium to allow accumulation of the L-amino acid in a culture and by collecting the L-amino acid from the culture, the bacterium being modified so that intracellular ppGpp synthesis ability is increased.
摘要翻译： 通过培养具有在培养基中产生L-氨基酸的能力的细菌产生L-氨基酸，以允许L-培养物中的L-氨基酸的积累，并从培养物中收集L-氨基酸，该细菌 被修饰以使细胞内ppGpp合成能力增加。
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公开(公告)号：US09644009B2
公开(公告)日：2017-05-09
申请号：US12055438
申请日：2008-03-26
IPC分类号： C12P13/04 , C07K14/245 , C12P13/08 , C12P13/22
CPC分类号： C07K14/245 , C12P13/04 , C12P13/08 , C12P13/22
摘要： An L-amino acid is produced by culturing a microorganism of the family Enterobacteriaceae which has the ability to produce an L-amino acid and which has been modified so as to increase the expression of the evgA gene, the gadE gene, and/or the ydeO gene. These genes encode a transcription factor involved in the EvgAS two-component system regulon. The culture takes place in a medium, and the L-amino acid is collected from the medium or cells.
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公开(公告)号：US09599618B2
公开(公告)日：2017-03-21
申请号：US12487009
申请日：2009-06-18
申请人： Akira Imaizumi , Toshihiko Ando , Naoyuki Okamoto
发明人： Akira Imaizumi , Toshihiko Ando , Naoyuki Okamoto
IPC分类号： G01N33/48 , G01N33/574
CPC分类号： G01N33/57419 , G01N33/57488
摘要： According to the method of evaluating colorectal cancer of the present invention, amino acid concentration data on the concentration value of amino acid in blood collected from a subject to be evaluated is measured, and a colorectal cancer state in the subject is evaluated based on the concentration value of at least one of Arg, Cys, Orn, Trp, Glu, ABA, Val, Phe, Leu, Gln, Ile and His contained in the measured amino acid concentration data of the subject.
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公开(公告)号：US09465031B2
公开(公告)日：2016-10-11
申请号：US12971419
申请日：2010-12-17
申请人： Naoyuki Okamoto , Takeshi Miura , Yohei Miyagi , Akira Imaizumi , Hiroshi Yamamoto , Takayuki Tanaka , Takahiko Muramatsu , Toshihiko Ando , Noriko Takahashi
发明人： Naoyuki Okamoto , Takeshi Miura , Yohei Miyagi , Akira Imaizumi , Hiroshi Yamamoto , Takayuki Tanaka , Takahiko Muramatsu , Toshihiko Ando , Noriko Takahashi
IPC分类号： G01N33/48 , G01N33/574 , G01N33/68
CPC分类号： G01N33/57434 , G01N33/6812 , G01N2800/342
摘要： According to the method of evaluating prostatic disease of the present invention, amino acid concentration data on concentration values of amino acids in blood collected from a subject to be evaluated is measured, and the state of prostatic disease including at least one of prostatic cancer and prostatic hypertrophy in the subject is evaluated based on the concentration value of at least one of Tau, Thr, Ser, Asn, Glu, Pro, Gly, Ala, Cit, ABA, Val, Met, Ile, Leu, Phe, His, Trp, Orn, Lys, and Cys2 contained in the measured amino acid concentration data of the subject.
摘要翻译： 根据本发明的前列腺疾病的评价方法，测定从被评价对象收集的血液中的氨基酸的浓度值的氨基酸浓度数据，前列腺疾病的状态，包括前列腺癌和前列腺中的至少一种 基于Tau，Thr，Ser，Asn，Glu，Pro，Gly，Ala，Cit，ABA，Val，Met，Ile，Leu，Phe，His，Trp中的至少一种的浓度值来评价受试者的肥大， Orn，Lys和Cys2包含在受试者的测量的氨基酸浓度数据中。
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公开(公告)号：US08394612B2
公开(公告)日：2013-03-12
申请号：US12184637
申请日：2008-08-01
申请人： Akira Imaizumi , Larisa Gotlibovna Airikh , Vera Georgievna Doroshenko , Irina Sergeevna Tsyrenzhapova
发明人： Akira Imaizumi , Larisa Gotlibovna Airikh , Vera Georgievna Doroshenko , Irina Sergeevna Tsyrenzhapova
IPC分类号： C12P13/22
CPC分类号： C12N15/70 , C12N1/38 , C12P13/08 , C12P13/14 , C12P13/222 , C12P13/227
摘要： A method is provided for producing an L-amino acid by culturing a microorganism belonging to the Enterobacteriaceae family and having the ability to produce an L-amino acid, in a medium to produce and accumulate the L-amino acid in the medium. The microorganism has been modified by introduction of a DNA fragment which includes a pho regulon promoter and a structural gene encoding an L-amino acid biosynthetic enzyme, which is ligated downstream of the promoter so that the gene is expressed by the promoter, and so that the activity of the L-amino acid biosynthetic enzyme is increased by the expression of the gene by the promoter. In this way, the L-amino acid that is produced in the medium can be collected. Furthermore, the phosphorus concentration in the medium is such that the expression of the gene by the promoter is induced.
摘要翻译： 提供了通过在培养基中培养属于肠杆菌科的微生物并具有产生L-氨基酸能力的培养基来生产和积累L-氨基酸的方法来生产L-氨基酸的方法。 通过引入包含光调节启动子和编码L-氨基酸生物合成酶的结构基因的DNA片段修饰微生物，该结构基因连接在启动子的下游，使得该基因由启动子表达，并且使得 L-氨基酸生物合成酶的活性通过启动子的表达而增加。 以这种方式，可以收集在培养基中产生的L-氨基酸。 此外，培养基中的磷浓度使得促进基因的表达被诱导。
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