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    Method and Apparatus for Amplifying Nucleic Acid Sequences
    13.
    发明申请
    Method and Apparatus for Amplifying Nucleic Acid Sequences 审中-公开
    用于扩增核酸序列的方法和装置

    公开(公告)号：US20110195458A1

    公开(公告)日：2011-08-11

    申请号：US13021379

    申请日：2011-02-04

    申请人： Dieter Braun ,  Christof Mast

    发明人： Dieter Braun ,  Christof Mast

    IPC分类号： C12P19/34 ,  C12M1/00

    CPC分类号： B01L3/502715 ,  B01L3/5027 ,  B01L3/50273 ,  B01L7/525 ,  B01L2200/0631 ,  B01L2300/0816 ,  B01L2300/0838 ,  B01L2300/1822 ,  B01L2300/1872 ,  B01L2400/0445 ,  B01L2400/0451 ,  B01L2400/0454 ,  C12Q1/686 ,  C12Q2527/15 ,  C12Q2527/101

    摘要： A method of amplifying one or more target nucleic acid sequence(s) in a PCR buffer solution comprising one or more target nucleic acid sequence(s), a nucleic acid polymerase, deoxynucleotide or nucleotide triphosphates, at least a first primer and a second primer is provided. The PCR buffer solution is contained in an elongated vessel. The solution within the vessel is locally heated in a spatio-temporally varying manner. Fluid flows circulating within the vessel are established. At least a fraction of the PCR buffer solution cyclically travels between regions having different temperatures, giving rise to a temperature cycling.

    摘要翻译： 一种在包含一个或多个靶核酸序列，核酸聚合酶，脱氧核苷酸或核苷酸三磷酸的PCR缓冲溶液中扩增一个或多个靶核酸序列的方法，至少第一引物和第二引物 被提供。 PCR缓冲溶液包含在细长的容器中。 容器内的溶液以时空变化的方式局部加热。 建立在容器内流动的流体流动。 至少一部分PCR缓冲溶液在具有不同温度的区域之间循环行进，产生温度循环。

    THERMO-OPTICAL CHARACTERISATION OF NUCLEIC ACID MOLECULES
    14.
    发明申请
    THERMO-OPTICAL CHARACTERISATION OF NUCLEIC ACID MOLECULES 有权
    核酸分子的热光学特征

    公开(公告)号：US20100330578A1

    公开(公告)日：2010-12-30

    申请号：US12865985

    申请日：2009-02-06

    申请人： Stefan Duhr ,  Philipp Baaske

    发明人： Stefan Duhr ,  Philipp Baaske

    IPC分类号： C12Q1/68 ,  G01N21/64 ,  C12M1/34

    CPC分类号： G01N21/6428 ,  B01L3/50273 ,  B01L2300/0822 ,  B01L2300/1861 ,  B01L2300/1872 ,  B01L2400/0451 ,  C12Q1/6813 ,  G01N21/171 ,  G01N21/6408 ,  G01N21/6458 ,  C12Q2527/107 ,  C12Q2523/313

    摘要： The present invention pertains to a method and a device for the determination of thermo-optical properties, particularly the size or size distribution, of fluorescently labeled biomolecules or biomolecule complexes, particularly nucleic acids, in a reaction solution. The method comprises the steps of: (i) providing a reaction solution with fluorescently labeled biomolecules or biomolecule complexes; (ii) irradiating a laser light beam into the solution to obtain a spatial temperature distribution in the solution around the irradiated laser light beam; (iii) exciting fluorescently said fluorescently labeled biomolecules and detecting the fluorescence at two or more defined regions representing different mean temperatures in said spatial temperature distribution, wherein said detection of fluorescence is performed at least once at a predetermined time after the start of the laser irradiation; and (iv) determining the thermo-optical properties, particularly the size or size distribution, of the fluorescently labeled biomolecules or biomolecule complexes from the detected fluorescence intensity or fluorescence intensity distribution.

    摘要翻译： 本发明涉及用于确定反应溶液中荧光标记的生物分子或生物分子复合物，特别是核酸的热光学性质，特别是尺寸或尺寸分布的方法和装置。 该方法包括以下步骤：（i）提供具有荧光标记的生物分子或生物分子复合物的反应溶液; （ii）将激光束照射到溶液中以获得围绕照射的激光束的溶液中的空间温度分布; （iii）激发荧光标记的荧光标记的生物分子，并检测在所述空间温度分布中表示不同平均温度的两个或多个限定区域处的荧光，其中所述荧光检测在激光照射开始之后的预定时间至少进行一次 ; 和（iv）根据检测到的荧光强度或荧光强度分布确定荧光标记的生物分子或生物分子复合物的热 - 光学性质，特别是尺寸或尺寸分布。

    Surface for reversible wetting-dewetting
    17.
    发明授权
    Surface for reversible wetting-dewetting 有权
    表面可逆湿润润湿

    公开(公告)号：US08287808B2

    公开(公告)日：2012-10-16

    申请号：US11227663

    申请日：2005-09-15

    申请人： Thomas Nikita Krupenkin ,  Joseph Ashley Taylor

    发明人： Thomas Nikita Krupenkin ,  Joseph Ashley Taylor

    IPC分类号： G01N15/06

    CPC分类号： B01L3/502792 ,  B01L2300/161 ,  B01L2400/0427 ,  B01L2400/0451 ,  Y10T428/249953

    摘要： An apparatus comprising a plurality of closed-cells on a substrate surface. Each of the closed-cells comprise one or more internal walls that divide an interior of each of the closed-cells into a single first zone and a plurality of second zones. The first zone occupies a larger area of the closed-cell than any one of said second zones and the first and second zones are interconnected to form a common volume.

    摘要翻译： 一种在衬底表面上包括多个闭孔的设备。 每个闭孔包括一个或多个将每个闭孔的内部分成单个第一区和多个第二区的内壁。 所述第一区占所述第二区中任何一个的所述闭孔的较大面积，并且所述第一和第二区被互连以形成公共体积。

    Polymer chain reaction apparatus using marangoni convection and polymer chain reaction method using the same
    20.
    发明申请
    Polymer chain reaction apparatus using marangoni convection and polymer chain reaction method using the same 审中-公开
    聚合物链反应装置采用马龙加对流和聚合物链反应方法

    公开(公告)号：US20060216725A1

    公开(公告)日：2006-09-28

    申请号：US11219182

    申请日：2005-09-02

    申请人： You-seop Lee ,  Keon Kuk ,  Yong-soo Oh ,  Su-ho Shin ,  Min-soo Kim

    发明人： You-seop Lee ,  Keon Kuk ,  Yong-soo Oh ,  Su-ho Shin ,  Min-soo Kim

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C12M1/34

    CPC分类号： B01L3/50273 ,  B01L3/0268 ,  B01L7/525 ,  B01L2200/0673 ,  B01L2300/0861 ,  B01L2300/087 ,  B01L2300/165 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2400/0442 ,  B01L2400/0448 ,  B01L2400/0451

    摘要： A polymerase chain reaction apparatus includes: a substrate; a high-temperature sidewall erected on the substrate; a low-temperature sidewall erected on the substrate and facing the high-temperature sidewall; and a reaction chamber consisting of the substrate, the high-temperature sidewall, and the low-temperature sidewall, wherein a sample contained in the reaction chamber is repetitively thermal-circulated between the high-temperature sidewall and the low-temperature sidewall using Marangoni convection generated by a surface tension gradient resulting from a temperature difference in an interface between the sample and air. The PCR amplification can be automatically accomplished by surface tension flow generated by Marangoni convection resulting from a temperature difference in an interface between the sample and air when a temperature difference between the sidewalls of the chamber is maintained constant. As a result, it is possible to reduce power consumption, simplify the configuration of a temperature control circuit, and reduce the time for a cycle of amplification.

    摘要翻译： 聚合酶链反应装置包括：基材; 竖立在基板上的高温侧壁; 竖立在基板上并面向高温侧壁的低温侧壁; 以及由衬底，高温侧壁和低温侧壁组成的反应室，其中包含在反应室中的样品使用Marangoni对流在高温侧壁和低温侧壁之间重复热循环 由样品和空气之间的界面的温度差引起的表面张力梯度产生。 当腔室的侧壁之间的温差保持不变时，可以通过由样品和空气之间的界面的温度差导致的Marangoni对流产生的表面张力流自动完成PCR扩增。 结果，可以降低功耗，简化温度控制电路的配置，并减少放大循环的时间。