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    Compositions And Methods For Delivery Of Agents Into Cells
    15.
    发明申请
    Compositions And Methods For Delivery Of Agents Into Cells 审中-公开
    用于将试剂递送到细胞中的组合物和方法

    公开(公告)号：US20080207544A1

    公开(公告)日：2008-08-28

    申请号：US11814180

    申请日：2006-01-25

    申请人： Sean M. Sullivan

    发明人： Sean M. Sullivan

    IPC分类号： A61K31/7115 ,  A61K31/683

    CPC分类号： C12N15/88 ,  A61K48/0025 ,  C12N2810/855

    摘要： Disclosed herein are novel compositions for delivery of biologically active agents into cells comprising a cationic amphiphile and a complementary lipid composition. Also disclosed are methods of making and using such novel compositions.

    摘要翻译： 本文公开了用于将生物活性剂递送到包含阳离子两亲物和互补脂质组合物的细胞中的新型组合物。 还公开了制备和使用这种新型组合物的方法。

    Methods and compositions for genetically modifying primate bone marrow cells
    17.
    发明申请
    Methods and compositions for genetically modifying primate bone marrow cells 审中-公开
    用于遗传修饰灵长类动物骨髓细胞的方法和组合物

    公开(公告)号：US20030166285A1

    公开(公告)日：2003-09-04

    申请号：US10164076

    申请日：2002-06-04

    发明人： Domenico Valerio ,  Victor Willem Van Beusechem

    IPC分类号： C12N015/867 ,  C12N005/08

    CPC分类号： C12N15/86 ,  A61K48/00 ,  C07K14/005 ,  C12N9/78 ,  C12N2740/13022 ,  C12N2740/13043 ,  C12N2740/13045 ,  C12N2810/6054 ,  C12N2810/855

    摘要： A method is provided for genetically modifying primate bone marrow cells, comprising isolating bone marrow cells from a primate and, by means which enhance the local concentration of retroviral particles, contacting the isolated bone marrow cells to cells that produce a recombinant amphotropic retrovirus with a genome based on a retroviral vector that contains the genetic information to be introduced into the bone marrow cells. Recombinant amphotropic retrovirus-producing cells, suitable for use in this method also are provided, as are genetically modified primate bone marrow cells with the capacity for regeneration in vivo.

    摘要翻译： 提供了用于遗传修饰灵长类动物骨髓细胞的方法，其包括从灵长类动物分离骨髓细胞，并且通过增强逆转录病毒颗粒的局部浓度的方法，使分离的骨髓细胞与产生具有基因组的重组顺势性逆转录病毒的细胞接触 基于包含要引入骨髓细胞的遗传信息的逆转录病毒载体。 还提供了适用于该方法的重组顺铂逆转录病毒产生细胞，以及具有体内再生能力的遗传修饰的灵长类动物骨髓细胞。

    Recombinant Rhabdovirus containing a heterologous fusion protein
    18.
    发明授权
    Recombinant Rhabdovirus containing a heterologous fusion protein 失效
    含有异源融合蛋白的重组狂犬病病毒

    公开(公告)号：US06497873B1

    公开(公告)日：2002-12-24

    申请号：US09217967

    申请日：1998-12-22

    申请人： Michael A. Whitt ,  Clinton S. Robison

    发明人： Michael A. Whitt ,  Clinton S. Robison

    IPC分类号： A01N6300

    CPC分类号： C12N15/86 ,  C07K14/005 ,  C07K2319/00 ,  C12N2760/18022 ,  C12N2760/20222 ,  C12N2760/20243 ,  C12N2810/855 ,  C12N2810/859 ,  C12N2840/20

    摘要： This invention relates to a composition comprising a recombinant or genetically engineered Rhabdovirus that expresses a Fusion Protein, such as the F protein of the Paramyxovirus SV5 strain. This recombinant Rhabdovirus may express other non-Rhabdovirus attachment proteins and/or an enhancer protein. The invention also relates to methods of making recombinant Rhabdoviruses which express an F Protein. These recombinant compositions can be used for purposes of research, as well as for diagnostic and therapeutic compositions for treatment of diseases.

    摘要翻译： 本发明涉及包含表达融合蛋白的重组或遗传工程化的Rhabdovirus的组合物，例如副粘病毒SV5株的F蛋白。 该重组Rhabdovirus可以表达其他非Rhabdovirus附着蛋白和/或增强子蛋白。 本发明还涉及制备表达F蛋白的重组杆状病毒的方法。 这些重组组合物可用于研究目的，以及用于治疗疾病的诊断和治疗组合物。

    Methods and compositions for genetically modifying primate bone marrow cells
    19.
    发明授权
    Methods and compositions for genetically modifying primate bone marrow cells 失效
    用于遗传修饰灵长类动物骨髓细胞的方法和组合物

    公开(公告)号：US06472212B1

    公开(公告)日：2002-10-29

    申请号：US08820479

    申请日：1997-03-18

    申请人： Domenico Valerio ,  Victor Willem Van Beusechem

    发明人： Domenico Valerio ,  Victor Willem Van Beusechem

    IPC分类号： C12N1586

    CPC分类号： C12N15/86 ,  A61K48/00 ,  C07K14/005 ,  C12N9/78 ,  C12N2740/13022 ,  C12N2740/13043 ,  C12N2740/13045 ,  C12N2810/6054 ,  C12N2810/855

    摘要： A method is provided for genetically modifying primate bone marrow cells, comprising isolating bone marrow cells from a primate and, by means which enhance the local concentration of retroviral contacting the isolated bone marrow cells to cells that produce a recombinant amphotropic retrovirus with a genome based on a retroviral vector that contains the genetic information to be introduced into the bone marrow cells. Recombinant amphotropic retrovirus-producing cells, suitable for use in this method also are provided, as are genetically modified primate bone marrow cells with the capacity for regeneration in vivo.

    摘要翻译： 提供了用于遗传修饰灵长类动物骨髓细胞的方法，其包括从灵长类动物分离骨髓细胞，并且通过增强逆转录病毒将分离的骨髓细胞与产生具有基因组的细胞接触的细胞的局部浓度的手段，所述基因组基于 包含要引入骨髓细胞的遗传信息的逆转录病毒载体。 还提供了适用于该方法的重组顺铂逆转录病毒产生细胞，以及具有体内再生能力的遗传修饰的灵长类动物骨髓细胞。

    Methods and means for targeted gene delivery
    20.
    发明申请
    Methods and means for targeted gene delivery 失效
    靶向基因传递的方法和手段

    公开(公告)号：US20010055586A1

    公开(公告)日：2001-12-27

    申请号：US09901836

    申请日：2001-07-10

    发明人： Domenico Valerio ,  Victor Willem Van Beusechem

    IPC分类号： A61K048/00

    CPC分类号： C07K14/005 ,  A61K47/6898 ,  A61K47/6901 ,  A61K48/00 ,  B82Y5/00 ,  C07K14/70535 ,  C07K2319/32 ,  C12N7/00 ,  C12N15/86 ,  C12N15/87 ,  C12N2710/00088 ,  C12N2740/13043 ,  C12N2740/13045 ,  C12N2740/13052 ,  C12N2740/15022 ,  C12N2810/855 ,  C12N2810/859

    摘要： A method for producing viral gene delivery vehicles which can be transferred to pre-selected cell types by using targeting conjugates. The gene delivery vehicles comprise: 1) the gene of interest; and 2) a viral capsid or envelope carrying a member of a specific binding pair, the counterpart of which is not directly associated with the surface of the target cell. These vehicles can be rendered unable to bind to their natural cell receptor. The targeting conjugates include the counterpart member of the specific binding pair, linked to a targeting moiety which is a cell-type specific ligand (or fragments thereof). The number of the specific binding pair present on the viral vehicles can be, for example, an immunoglobulin binding moiety (e.g., capable of binding to a Fc fragment, protein A, protein G, FcR or an anti-Ig antibody), or biotin, avidin or streptavidin. The virus' outer membrane or capsid may contain a substance which mediates entrance of the gene delivery vehicle into the target cell. Due to the specificity of the ligand, the binding pair's high affinity, and the gene delivery vehicle's inability to be targeted when used alone, the universality of the method for gene delivery, together with its high cell type selectively can be achieved by using various targeting conjugates.

    摘要翻译： 用于产生病毒基因递送载体的方法，其可以通过使用靶向缀合物转移到预先选择的细胞类型。 基因递送载体包括：1）感兴趣的基因; 和2）携带特异性结合对的成员的病毒衣壳或信封，其对应物不与靶细胞的表面直接相关。 这些载体可能不能与其天然细胞受体结合。 靶向缀合物包括与作为细胞型特异性配体（或其片段）的靶向部分连接的特异性结合对的对应成员。 存在于病毒载体上的特异性结合对的数目可以是例如免疫球蛋白结合部分（例如，能够结合Fc片段，蛋白A，蛋白G，FcR或抗-Ig抗体）或生物素 ，抗生物素蛋白或链霉亲和素。 病毒的外膜或衣壳可以含有介导基因递送载体进入靶细胞的入口的物质。 由于配体的特异性，结合对的高亲和力和基因递送载体在单独使用时不能被靶向，所以基因递送方法的普及性及其高细胞类型选择性可以通过使用各种靶向 共轭物