公开(公告)号：US20210071151A1

公开(公告)日：2021-03-11

申请号：US17084405

申请日：2020-10-29

申请人： Codexis, Inc.

发明人： Fabien Louis Cabirol ,  Haibin Chen ,  Anupam Gohel ,  Steven J. Collier ,  Derek J. Smith ,  Birgit Kosjek ,  Jacob Janey

IPC分类号： C12N9/04 ,  C12P41/00 ,  C12P13/00 ,  C12P13/02

摘要： The present disclosure relates to engineered ketoreductase polypeptides for the preparation of hydroxyl substituted carbamate compounds, and polynucleotides, vectors, host cells, and methods of making and using the ketoreductase polypeptides.

公开(公告)号：US20210024905A1

公开(公告)日：2021-01-28

申请号：US17043339

申请日：2019-03-29

申请人： API CORPORATION

发明人： Toyokazu YOSHIDA ,  Koichi ISHIDA ,  Ryoma MIYAKE ,  Takanobu IURA ,  Hiroshi KAWABATA

IPC分类号： C12N9/18 ,  C12P41/00 ,  C12P7/62

摘要： The present invention provides a novel hydrolase that can industrially produce optically highly pure (1S,2S)-1-alkoxycarbonyl-2-vinylcyclopropane carboxylic acid with high efficiency at low costs, and a production method using the hydrolase.

公开(公告)号：US20200347365A1

公开(公告)日：2020-11-05

申请号：US16874574

申请日：2020-05-14

申请人： Codexis, Inc.

发明人： Oscar Alvizo ,  Nicholas J. Agard ,  Xinkai Xie ,  David Entwistle ,  Birgit Kosjek

IPC分类号： C12N9/04 ,  C12P41/00 ,  C12P7/02 ,  C12P17/12 ,  C12P7/40

摘要： The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.

公开(公告)号：US10822627B2

公开(公告)日：2020-11-03

申请号：US15952377

申请日：2018-04-13

申请人： EVONIK TECHNOCHEMIE GMBH

发明人： Steffen Osswald ,  Heiko Schuster ,  Jurgen Roos ,  Andreas Karau ,  Ulrich Schwaneberg ,  Ronny Martinez ,  Hemanshu Mundhada ,  Ursula Holter

IPC分类号： C12N15/55 ,  C12N9/86 ,  C12P13/22 ,  C12P13/04 ,  C12P41/00

摘要： The present invention relates to a hydantoinase having an amino acid sequence selected from (i) or (ii), with (i) amino acid sequence selected from SEQ ID NO: 6-20 and SEQ ID NO: 73-119 (ii) amino acid sequence wherein in the amino acid sequence of SEQ ID NO: 6-20 and SEQ ID NO: 73-119, 1 to 75 amino acid residues have been substituted, deleted, inserted and/or added, and wherein further the catalytic activity of the hydantoinase is higher by a factor of at least 1.2 than the catalytic activity of the hydantoinase having amino acid sequence SEQ ID NO: 1. The present invention further relates to a process for preparing amino acids, wherein said hydantoinase is used.

公开(公告)号：US10696953B2

公开(公告)日：2020-06-30

申请号：US15543535

申请日：2016-02-05

申请人： Codexis, Inc.

发明人： Oscar Alvizo ,  Nicholas J. Agard ,  Xinkai Xie ,  David Entwistle ,  Birgit Kosjek

IPC分类号： C12N9/04 ,  C12P41/00 ,  C12P7/02 ,  C12P17/12 ,  C12P7/40

摘要： The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.

公开(公告)号：US10604781B2

公开(公告)日：2020-03-31

申请号：US16221223

申请日：2018-12-14

申请人： Codexis, Inc.

发明人： Fabien Louis Cabirol ,  Steven J. Collier ,  Thomas Daussmann ,  Naga K. Modukuru

IPC分类号： C12P13/04 ,  C12P13/06 ,  C12N9/02 ,  C12P41/00 ,  C12N9/04 ,  C12N9/06 ,  C12P7/40 ,  C12N9/08

摘要： The present disclosure relates to the use of an amino acid dehydrogenase in combination with a cofactor regenerating system comprising a ketoreductase. In particular embodiments, the process can be used to prepare L-tert-leucine using a leucine dehydrogenase.

公开(公告)号：US10550370B2

公开(公告)日：2020-02-04

申请号：US16399488

申请日：2019-04-30

申请人： Codexis Inc.

发明人： Ish K. Dhawan ,  Gregory Miller ,  Xiyun Zhang

IPC分类号： C12N9/10 ,  C12P13/00 ,  C12P41/00

摘要： The present disclosure provides engineered transaminase enzymes having improved properties as compared to a naturally occurring wild-type transaminase enzyme. Also provided are polynucleotides encoding the engineered transaminase enzymes, host cells capable of expressing the engineered transaminase enzymes, and methods of using the engineered transaminase enzymes to synthesize a variety of chiral compounds.

公开(公告)号：US20200024621A1

公开(公告)日：2020-01-23

申请号：US16435000

申请日：2019-06-07

申请人： AbbVie Inc.

发明人： Michael J. Abrahamson ,  Angelica B. Kielbus ,  William T. Riordan ,  David R. Hill ,  Sanjay R. Chemburkar ,  Rajarathnam E. Reddy ,  Timothy B. Towne ,  Jianzhang Mei ,  Stefan Mix ,  Gareth J. Brown

IPC分类号： C12P7/62 ,  C12P41/00

摘要： Disclosed are methods of synthesizing enantioenriched difluoroalkylcyclopropyl amino esters and their salts, such as the dicyclohexylamine salt of (1S,2R)-2-(difluoromethyl)-1-(propoxycarbonyl)cyclopropane carboxylic acid. These compounds are useful intermediates in the synthesis of viral protease inhibitors.

公开(公告)号：US10457648B2

公开(公告)日：2019-10-29

申请号：US15514188

申请日：2015-09-25

申请人： F. HOFFMANN-LA ROCHE AG ,  GENENTECH, INC.

发明人： Francis Gosselin ,  Chong Han ,  Hans Iding ,  Reinhard Reents ,  Scott Savage ,  Beat Wirz

IPC分类号： C07D239/70 ,  C07D239/54 ,  C07D239/42 ,  C07C253/30 ,  C07C255/22 ,  C07B57/00 ,  C12P41/00 ,  C07C253/34 ,  C12N9/78

摘要： The present disclosure relates to processes for preparing (cyclopentyl[d]pyrimidin-4-yl)piperazine compounds, and more particularly relates to processes for preparing (R)-4-(5-methyl-7-oxo-6,7-dihydro-5H-cyclopenta[d] pyrimidin-4-yl)piperazine and N-protected derivatives thereof, which may be used as an intermediate in the synthesis of Ipatasertib (i.e., (S)-2-(4-chlorophenyl)-1-(4-((5R,7R)-7-hydroxy-5-methyl-6,7-dihydro-5H-cyclopenta[d]pyrimidin-4-yl)piperazin-1-yl)-3-(isopropylamino)-propan-1-one). The present disclosure additionally relates to various compounds that are intermediates employed in these processes.

公开(公告)号：US20190323051A1

公开(公告)日：2019-10-24

申请号：US16451838

申请日：2019-06-25

申请人： ANNIKKI GMBH

发明人： Ortwin Ertl ,  Nicole Staunig ,  Marta Sut ,  Bernd Mayer

IPC分类号： C12P19/36 ,  C12P33/00 ,  C12P19/02 ,  C12P41/00

摘要： A process for the enzymatic regeneration of the redox cofactors NAD+/NADH and NADP+/NADPH in a one-pot reaction, wherein, as a result of at least two further enzymatically catalyzed redox reactions proceeding in the same reaction batch (product-forming reactions), one of the two redox cofactors accumulates in its reduced form and, respectively, the other one in its oxidized form, characterized in that a) in the regeneration reaction which reconverts the reduced cofactor into its original oxidized form, oxygen or a compound of general formula R1C(O)COOH is reduced, and b) in the regeneration reaction which reconverts the oxidized cofactor into its original reduced form, a compound of general formula R2CH(OH)R3 is oxidized and wherein R1, R2 and R3 in the compounds have different meanings.