公开(公告)号：US07297497B2

公开(公告)日：2007-11-20

申请号：US11431997

申请日：2006-05-08

Applicant: Jean I. Montagu ,  Roger Dowd ,  David Root

Inventor： Jean I. Montagu ,  Roger Dowd ,  David Root

IPC: C12Q1/68

CPC classification number: G01N33/544 ,  G01N33/54366

Abstract: An immobilizing device for biological material comprises a rigid support (12) carrying a substrate layer (20, 20′) of polymer having biological immobilizing properties, e.g. for amino and nucleic acids. Substantially solid ultra-thin substrate layers (20′) having a thickness less than about 5 micron, preferably between about 0.1 and 0.5 micron, and micro-porous, ultra-thin substrate layers (20′) having a thickness less than about 5 micron, preferably less than 3 micron, 2 or 1 micron are shown, which may be segmented by isolating moats M. The substrate layer is on a microscope slide (302), round disc (122), bio-cassette, at the bottom of a well of a multiwell plate, and as a coating inside a tube. Fluorescence or luminescence intensity and geometric calibration spots (420) are shown. Reading is enhanced by the intensity calibration spots. (420) to enable normalization of readings under uneven illumination conditions, as when reading by dark field, side illumination mode. The reference spots are shown being printed simultaneously with printing an array of biological spots or with the same equipment. Methods of forming layers of the device include controlled drawing from a bath of coating composition and drying, and spinning of C-D shaped substrates. Post-forming treatment is shown by corona treatment and radiation. Adherent metal oxides (14), silica-based materials and other materials are used to unite layers of the composite. In multi-well plates the oxide promotes joining of a bottom plate (95, 95′) and upper, well-defining structure (94) of dissimilar material. The oxides (14) also provide beneficial opacity to prevent light entering the glass support, for applying potential to the substrate, etc.

Abstract translation: 用于生物材料的固定装置包括承载具有生物固定性质的聚合物的基底层（20,20'）的刚性支撑体（12），例如。 用于氨基和核酸。 具有厚度小于约5微米，优选约0.1至0.5微米的基本上固体的超薄基底层（20'）和厚度小于约5微米的微孔超薄基底层（20'） ，优选小于3微米，2或1微米，其可以通过分离护羊M分段。基底层在显微镜载玻片（302），圆盘（122），生物盒，底部 多孔板，以及管内的涂层。 显示荧光或发光强度和几何校准点（420）。 通过强度校准点增强读数。 （420），以便在不均匀照射条件下使读数标准化，如通过暗场，侧面照明模式读取。 参考点被显示为同时印刷生物斑点阵列或用相同的设备打印。 形成装置层的方法包括从涂料组合物的浴中进行控制拉伸和干燥，以及C-D形基材的纺丝。 后处理通过电晕处理和辐射显示。 使用粘附金属氧化物（14），二氧化硅基材料和其他材料来组合复合材料层。 在多孔板中，氧化物促进底板（95,95'）和不同材料的上部，良好限定结构（94）的接合。 氧化物（14）还提供有益的不透明性，以防止光进入玻璃支撑体，以将电位施加到基底等。

公开(公告)号：US20050251376A1

公开(公告)日：2005-11-10

申请号：US10843532

申请日：2004-05-10

Applicant: Alexander Pekker ,  David Root ,  John Wood

Inventor： Alexander Pekker ,  David Root ,  John Wood

IPC: G06F17/50

CPC classification number: G06F17/5036

Abstract: Operation of an electronic circuit is simulated. A plurality of data points representing operation of the electronic circuit at a plurality of operating conditions is obtained. A boundary that encompasses the plurality of data points is constructed. The boundary defines a bounded domain of operating conditions for the electronic circuit. A model function representing operation of the electronic circuit within and without the bounded domain of operating conditions is produced. The model function is determined within the bounded domain of operating conditions. The model function is extended outside the bounded domain so that all first order partial derivatives of the model function for operating conditions located outside and not near the boundary are large and positive. The model function is utilized to simulate operation of the electronic circuit.

Abstract translation: 模拟电子电路的运行。 获得表示多个操作条件下的电子电路的动作的多个数据点。 构成包含多个数据点的边界。 该边界定义了用于电子电路的有界区域的操作条件。 产生表示操作条件的有界区域内的电子电路的运行的模型函数。 模型函数在操作条件的有界域内确定。 模型函数在有界域外扩展，使得位于外部且不在边界附近的操作条件的模型函数的所有一阶偏导数都是大的和正的。 该模型函数用于模拟电子电路的运行。

公开(公告)号：US20050102124A1

公开(公告)日：2005-05-12

申请号：US10712706

申请日：2003-11-12

Applicant: David Root ,  Nicholas Tuffilaro ,  John Wood ,  Jan Verspecht

Inventor： David Root ,  Nicholas Tuffilaro ,  John Wood ,  Jan Verspecht

IPC: G06F17/50

CPC classification number: G06F17/5036

Abstract: The present invention includes a method for generating a model of a circuit having an input port and an output port. The method determines an amplitude for current leaving the output port at a frequency ωk when a signal that includes a carrier at ωj modulated by a signal Vj(t) is input to the input port, wherein ωk is a harmonic of ωj. The determined amplitude is used to determine values for a set of constants, ak, such that a function fk(V,ak) provides an estimate of the current, Ik(t), leaving the output port at a frequency ωk when a signal having the form V ⁡ ( t ) = Re ⁢ ∑ k = 1 , H ⁢ V k ⁡ ( t ) ⁢ exp ⁡ ( jω k ⁢ t ) is input to the input port. Here Vk(t) is a component of a set of values V. The fk(V,ak) are used to provide a simulator component adapted for use in a circuit simulator.

公开(公告)号：US06350618B1

公开(公告)日：2002-02-26

申请号：US09300121

申请日：1999-04-27

Applicant: Nicholas F. Borrelli ,  Alain R. E. Carre ,  Thierry L. A. Dannoux ,  Bernard Eid ,  David Root ,  Raja Rao Wusirika

Inventor： Nicholas F. Borrelli ,  Alain R. E. Carre ,  Thierry L. A. Dannoux ,  Bernard Eid ,  David Root ,  Raja Rao Wusirika

IPC: G01N100

CPC classification number: G01N1/00 ,  B01J19/0046 ,  B01J2219/00274 ,  B01J2219/0031 ,  B01J2219/00315 ,  B01J2219/00317 ,  B01J2219/00369 ,  B01J2219/00378 ,  B01J2219/005 ,  B01J2219/00511 ,  B01J2219/00547 ,  B01J2219/00549 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00673 ,  B01J2219/00722 ,  B01L3/0244 ,  B01L3/0262 ,  B01L3/50857 ,  B01L3/563 ,  B01L2200/021 ,  B01L2300/0838 ,  C03B19/06 ,  C03B23/047 ,  C03B23/06 ,  C03B37/028 ,  C40B40/06 ,  C40B60/14 ,  C40B70/00 ,  G01N33/54306 ,  G01N35/1065 ,  G01N35/1067 ,  G01N2035/00237 ,  G01N2035/1037 ,  G01N2035/1069 ,  Y10T436/25 ,  Y10T436/2575

Abstract: Methods and apparatus for depositing a high density biological or chemical array onto a solid support. Specifically, the apparatus is made up of a plurality of open ended channels collectively forming a matrix. The matrix has been redrawn and cut such that the pitch of the channels on the loading end is larger than the pitch of the channels on the liquid delivery end. The upper portion of each channel serves as a reservoir, while the opposing end, which has been formed by the redrawing process, is diametrically sized such that liquid in the reservoir is retained by capillary pressure at the delivery end. At any point along the height of the capillary reservoir device, all cross-sectional dimensions and areas are uniformly reduced. In other words, the on-center orientation of any two channels, also referred to as the pitch between 2 channels, measured as a function of the diameter of any cross section, is constant throughout the structure. The liquid within the channels is either printed directly from the tool onto a substrate or transferred to a substrate by a typographical pin plate. In another embodiment, the device may be used in transferring sample between multiwell plates of different well density.

Abstract translation: 将高密度生物或化学阵列沉积在固体支持物上的方法和装置。 具体地说，该装置由多个共同形成矩阵的开放式通道构成。 已经重新绘制和切割基质，使得加载端上的通道的间距大于液体输送端上的通道的间距。 每个通道的上部用作储存器，而通过重画过程形成的相对端的直径尺寸使得储存器中的液体在输送端处被毛细管压力保持。 在毛细管储存装置的高度的任何一点上，所有横截面尺寸和面积均匀地减小。 换句话说，任何两个通道的中心定向，也称为两个通道之间的间距，作为任何横截面直径的函数而测量，在整个结构中是恒定的。 通道内的液体直接从工具印刷到基底上，或者通过印刷销板转印到基底上。 在另一个实施方案中，该装置可用于在不同孔密度的多孔板之间转移样品。

公开(公告)号：US5650323A

公开(公告)日：1997-07-22

申请号：US721250

申请日：1991-06-26

Applicant: David Root

Inventor： David Root

IPC: B01L3/00 ,  C12M1/20 ,  C12M1/34 ,  C12M3/00

CPC classification number: B01L3/50853 ,  B01L3/5085 ,  C12M23/12

Abstract: A laboratory research tool for conducting a variety of procedures including cell and tissue culture techniques includes a multi-well cluster plate, a filter plate that has one or more filter wells that extend into the wells of the cluster plate, a cover and a reservoir that can be used with auxiliary equipment designed for use with standard 96-well format cluster plates. The system allows multiple tissue samples to be grown and manipulated simultaneously.

Abstract translation: 用于进行各种程序（包括细胞和组织培养技术）的实验室研究工具包括多孔聚集板，具有延伸到簇板的孔中的一个或多个过滤器孔的过滤板，盖和储存器， 可用于与标准96孔格式集群板一起使用的辅助设备。 该系统允许同时生长和操作多个组织样本。

公开(公告)号：US5272083A

公开(公告)日：1993-12-21

申请号：US705767

申请日：1991-05-28

Applicant: David Butz ,  George Lyman ,  David Root ,  Gregory Mathus

Inventor： David Butz ,  George Lyman ,  David Root ,  Gregory Mathus

IPC: C12M3/00 ,  C12M3/06 ,  C12N5/00 ,  C12N5/06

CPC classification number: C12M47/02 ,  C12M23/00 ,  C12M23/12 ,  C12M25/04

Abstract: A tissue or cell growth device is described for placement within a well of a cluster dish. The device has a cell or tissue retention element detachably attached to a hanger. The hanger preferably has openings allowing access to the well without removal of the member and is capable of positioning the cell or tissue retention element a preselected distance relative to the hanger. Also described are various embodiments of the device having differently shaped hangers and the combination of the device and a cluster dish.

Abstract translation: 描述了组织或细胞生长装置用于放置在簇盘的孔内。 该装置具有可拆卸地附接到衣架的细胞或组织保持元件。 衣架优选地具有允许进入井的开口，而不移除构件，并且能够将电池或组织保持元件相对于衣架定位成预选的距离。 还描述了具有不同形状的悬挂器以及装置和簇盘的组合的装置的各种实施例。

公开(公告)号：US4895706A

公开(公告)日：1990-01-23

申请号：US187083

申请日：1988-04-28

Applicant: David Root ,  George Lyman

Inventor： David Root ,  George Lyman

IPC: G01N1/10 ,  B01D29/01 ,  B01D61/18 ,  B01D63/08 ,  B01L3/00 ,  B01L9/06 ,  G01N21/03

CPC classification number: B01L9/06 ,  B01D61/18 ,  B01L3/5025 ,  B01L3/50255 ,  B01L3/50855 ,  G01N2021/0357 ,  G01N2201/0446 ,  Y10S436/809 ,  Y10T436/255

Abstract: A filter strip and composite assemblies for filtering microliter quantities of fluids wherein a linear array of wells having open top and bottom ends are connected by frangible webs in spaced-apart relation with discrete filter membranes closing the bottom ends of each well. Tabs are provided on the ends of the filter strip for holding the same and supporting the strip in a rectangular holder having alphanumeric designations for identifying each well in a plurality of such filter strips contained within the holder. The filter strip may be used in a vacuum manifold for applying a pressure differential across the filter membrane and directing the filtrate into an aligned aperture of a closed bottom well of an array of wells held within the manifold. Alternatively, the filter strip may be used with a transfer plate for directing the filtrate from each well of the filter strip to an aligned well of a closed bottom array of wells. Another composite assembly includes a pressure manifold for applying an increased pressure above the membranes.

Abstract translation: 用于过滤微量流体的过滤条和复合组件，其中具有开口顶部和底部的井的线性阵列通过易碎的腹板以与闭合每个孔的底端的离散过滤膜隔开的关系连接。 在过滤条的端部设置有用于保持其的突片，并将条带支撑在具有字母数字标号的矩形保持器中，用于识别容纳在保持器内的多个这种过滤条中的每个孔。 过滤条可以用在真空歧管中，用于在过滤膜上施加压力差，并将滤液引导到保持在歧管内的阱阵列的封闭的底部井的对准孔。 或者，过滤条可以与转移板一起使用，用于将滤液从过滤条的每个孔导向孔的封闭底部阵列的对准井。 另一复合组件包括用于在膜上施加增加压力的压力歧管。