公开(公告)号：US20090124876A1

公开(公告)日：2009-05-14

申请号：US11994010

申请日：2006-06-27

申请人： Hideaki Nakamura ,  Masao Gotoh ,  Isao Karube

发明人： Hideaki Nakamura ,  Masao Gotoh ,  Isao Karube

IPC分类号： A61B5/157 ,  A61B5/055

CPC分类号： A61B10/0045 ,  A61B5/150022 ,  A61B5/150099 ,  A61B5/150282 ,  A61B5/150358 ,  A61B5/150435 ,  A61B5/150503 ,  A61B5/150572 ,  A61B5/15113 ,  A61B5/15117 ,  A61B5/1519 ,  A61B5/15194 ,  A61B5/157 ,  A61B2562/0295

摘要： There is a needle integrated biosensor, including: a biosensor including at least two electrodes, and a puncture needle to collect a body fluid by piercing a skin of a person to be tested, the biosensor and the puncture needle being integrated, wherein the puncture needle is able to be kept hygienic without the need of a protective cover or the like for the puncture needle.There is a needle integrated biosensor, including: a biosensor including at least two electrodes, and a puncture needle to collect a body fluid by piercing a skin of a person to be tested, the biosensor and the puncture needle being integrated, wherein a soft material is attached to the front end of the puncture needle so that the front end of the puncture needle is protected.

摘要翻译： 针组合生物传感器包括：生物传感器，包括至少两个电极;以及穿刺针，用于通过刺穿待测试人员的皮肤来收集体液，所述生物传感器和穿刺针是一体的，其中所述穿刺针 能够保持卫生，而不需要用于穿刺针的保护罩等。 针组合式生物传感器包括：生物传感器，包括至少两个电极;以及穿刺针，用于通过刺穿待测试人的皮肤，生物传感器和穿刺针一体地收集体液，其中柔软材料 附着在穿刺针的前端，使得穿刺针的前端受到保护。

公开(公告)号：US20090053105A1

公开(公告)日：2009-02-26

申请号：US11795893

申请日：2006-01-23

申请人： Toshifumi Hosoya ,  Shingo Kaimori ,  Moriyasu Ichino ,  Hideaki Nakamura ,  Masao Gotoh ,  Fumiyo Kurusu ,  Tomoko Ishikawa ,  Isao Karube

发明人： Toshifumi Hosoya ,  Shingo Kaimori ,  Moriyasu Ichino ,  Hideaki Nakamura ,  Masao Gotoh ,  Fumiyo Kurusu ,  Tomoko Ishikawa ,  Isao Karube

IPC分类号： B01J19/00

CPC分类号： G01N33/54373 ,  B01L3/502707 ,  B01L2300/0645 ,  B01L2300/0822 ,  B01L2300/168

摘要： There is provided a sensor chip including: a base plate; a cover layer; a hollow reaction portion provided between the base plate and the cover layer, in which a reaction is performed between a sample and a reagent applied thereinto; a sensing member constructed of an electrode which is exposed to the hollow reaction portion; and a sample inlet for conducting the sample to the hollow reaction portion, wherein entire portions of the base plate and the cover layer, which are located opposite to the sensing member provided with the hollow reaction portion, are made transparent. Otherwise, in the above-described sensor chip, a plate A having a groove A to which a reagent has been applied and a plate B having a groove B are stacked on each other in such a manner that the groove A is coupled with the groove B, so that the hollow reaction portion is formed; and an opening width of the groove B is larger than an opening width of the groove A at a portion of the groove B which is located opposite to a portion with the groove A, to which the reagent has been applied.

摘要翻译： 提供了一种传感器芯片，包括：基板; 覆盖层; 设置在基板和覆盖层之间的中空反应部分，其中在样品和施加于其之间的试剂之间进行反应; 由暴露于中空反应部分的电极构成的传感部件; 以及用于将样品导引到中空反应部分的样品入口，其中与设置有中空反应部分的感测构件相对的基板和覆盖层的整个部分被制成透明的。 另外，在上述传感器芯片中，将具有涂敷有试剂的槽A的板A和具有槽B的板B彼此堆叠，使得槽A与槽 B，形成中空反应部分; 并且槽B的开口宽度大于槽B的与已经施加有试剂的部分的与槽A相对的部分处的槽A的开口宽度。

公开(公告)号：US20090020420A1

公开(公告)日：2009-01-22

申请号：US11921166

申请日：2006-10-16

申请人： Takahiko Kitamura ,  Toshifumi Hosoya ,  Shingo Kaimori ,  Moriyasu Ichino ,  Hideaki Nakamura ,  Isao Karube ,  Masao Gotoh ,  Tomoko Ishikawa

发明人： Takahiko Kitamura ,  Toshifumi Hosoya ,  Shingo Kaimori ,  Moriyasu Ichino ,  Hideaki Nakamura ,  Isao Karube ,  Masao Gotoh ,  Tomoko Ishikawa

IPC分类号： G01N27/327 ,  B05D5/00

CPC分类号： C12Q1/002

摘要： It is intended to provide a biosensor chip capable of rapid and correct measurement, including a reaction chamber that enables a measurement of a very small amount of a measurement sample and has a small capacity and potassium ferricyanide having a very small crystal particle size disposed in the reaction chamber.In a biosensor chip 1, electrodes 3 and 4 are disposed on the lower substrate 2, and lower spacer 5 (7 and 8) is adhered on the electrodes 3 and 4. An upper spacer 11 (12 and 13) is adhered to an upper substrate 15, and a lower spacer 5 is attached to the upper spacer 11 by and adhesive agent 10. A lower groove 9 is formed between long and short lower spacers 7 and 8, and an upper groove 14 is formed between long and short spacers 12 and 13, so that a reaction chamber 17 is formed by the upper and lower grooves 9 and 14. A capacity of the reaction chamber is 0.3 μL, and an enzyme 18 and potassium ferricyanide are disposed in the reaction chamber 17 in such a fashion as to oppose to each other with a gap being defined therebetween. A crystal particle diameter of the potassium ferricyanide is 100 μm or less, and amount of the potassium ferricyanide is V×0.1 mg or more when the capacity of the reaction chamber 17 is V.

摘要翻译： 旨在提供能够快速且正确测量的生物传感器芯片，其包括能够测量非常少量的测量样品并且具有小容量的反应室和具有非常小的晶粒度的铁氰化钾 反应室。 在生物传感器芯片1中，电极3和4设置在下基板2上，下隔片5（7和8）粘附在电极3和4上。上间隔件11（12和13） 基板15和下隔板5通过粘合剂10附接到上间隔件11.下槽9形成在长和短下隔板7和8之间，并且上槽14形成在长间隔件12和短间隔件12之间 和13，使得反应室17由上下槽9和14形成。反应室的容量为0.3微米，酶18和铁氰化钾以如下方式设置在反应室17中： 彼此相对，在其间限定间隙。 当反应室17的容量为V时，铁氰化钾的晶粒直径为100μm以下，铁氰化钾的量为Vx0.1mg以上。

公开(公告)号：US20070160298A1

公开(公告)日：2007-07-12

申请号：US11485626

申请日：2006-07-13

申请人： Tomokazu Murakami ,  Hiroaki Ito ,  Isao Karube ,  Masashi Takahashi

发明人： Tomokazu Murakami ,  Hiroaki Ito ,  Isao Karube ,  Masashi Takahashi

IPC分类号： G06K9/36

CPC分类号： H04N19/59 ,  H04N19/137 ,  H04N19/14 ,  H04N19/147 ,  H04N19/17 ,  H04N19/176 ,  H04N19/196 ,  H04N19/30 ,  H04N19/46 ,  H04N19/463 ,  H04N19/61 ,  H04N19/80 ,  H04N19/85

摘要： The present invention provides a technique capable performing encoding with a high image quality and a low bit rate. An image encoder according to the present inventions includes: an image separation unit for separating a skeleton image and a texture image from an inputted image; a skeleton image encoding unit for encoding the skeleton image separated; a texture image encoding unit for encoding the texture image separated; a parameter adjustment unit for adjusting a parameter used in encoding processing of the skeleton image and the texture image; a parameter encoding unit for encoding the parameter; and a code multiplexing unit for multiplexing the skeleton image, the texture image, and the parameter which have been encoded.

摘要翻译： 本发明提供能够以高图像质量和低比特率进行编码的技术。 根据本发明的图像编码器包括：图像分离单元，用于从输入的图像中分离骨架图像和纹理图像; 骨架图像编码单元，用于对分离的骨骼图像进行编码; 纹理图像编码单元，用于对分离的纹理图像进行编码; 参数调整单元，用于调整在骨架图像和纹理图像的编码处理中使用的参数; 用于对所述参数进行编码的参数编码单元; 以及用于多路复用已编码的骨架图像，纹理图像和参数的代码复用单元。

公开(公告)号：US20070000776A1

公开(公告)日：2007-01-04

申请号：US10565830

申请日：2004-04-15

申请人： Isao Karube ,  Masao Gotoh ,  Hideaki Nakamura

发明人： Isao Karube ,  Masao Gotoh ,  Hideaki Nakamura

IPC分类号： G01N33/487

CPC分类号： G01N27/3272

摘要： A downsized biosensor in which an electrode is formed on one sheet of electrically-insulated substrate, and a sheet of flat substrate is worked three-dimensionally so as to dispose the electrode on the inner side of the substrate to provide a two-dimensional or three-dimensional electrode disposition, whereby a sample introduced by capillary phenomenon can be measured quantitatively in a small space. A production method of a biosensor comprising the steps of bending a plate member so as to position an electrode formed on the surface of the electrically-insulated plane member on the inner side, and disposing the electrode in a space surrounded by a substrate and a cover to thereby form the substrate and the cover from one sheet of plate member.

摘要翻译： 在一片电绝缘基板上形成电极的小型生物传感器，三维地加工一层平面基板，以将电极设置在基板的内侧，以提供二维或三维 通过毛细管现象引入的样品可以在很小的空间内定量测量。 一种生物传感器的生产方法，包括以下步骤：弯曲板构件，以将形成在电绝缘平面构件的表面上的电极定位在内侧，并将电极设置在由基板和盖子 从而从一片板构件形成基板和盖。

公开(公告)号：US20050019922A1

公开(公告)日：2005-01-27

申请号：US10836773

申请日：2004-04-30

申请人： Isao Karube ,  Takashi Saitoh

发明人： Isao Karube ,  Takashi Saitoh

IPC分类号： C12M3/00 ,  C12N13/00 ,  C12N15/89 ,  C12N15/01 ,  C12N15/74

CPC分类号： C12N15/89 ,  C12M35/00 ,  C12N13/00

摘要： A technique for controlling membrane denaturation reactions other than physical shear force was developed. For example, the present invention provides, a method for causing membrane disruption at a specific site by reacting a stimulus such as light with a compound that is activated by the stimulus, where the reaction occurs on a membrane such as a biomembrane. It also provides a membrane structure such as cells in which a specific site has been disrupted, which are obtained by the present method. Introduction of substances such as genes also became possible by using this membrane structure. Further provided is a membrane-destroying member for disrupting a membrane at a specific site. Thus, the present invention enabled, for example, easy membrane penetration using components constituting microelectrodes, micromanipulators, and microinjectors, which were conventionally hardly usable in penetrating cell membranes.

摘要翻译： 开发了除物理剪切力之外控制膜变性反应的技术。 例如，本发明提供了通过使诸如光的刺激与由刺激激活的化合物反应而使特定部位的膜破裂的方法，其中反应发生在诸如生物膜的膜上。 还提供了通过本方法获得的膜结构，例如其中特异性位点已被破坏的细胞。 使用这种膜结构也可以引入基因等物质。 还提供了用于破坏特定部位的膜的破坏薄膜的部件。 因此，本发明能够例如使用通常几乎不能用于穿透细胞膜的微电极，显微操纵器和微注射器的构成的薄膜穿透。

公开(公告)号：US20030165953A1

公开(公告)日：2003-09-04

申请号：US10334831

申请日：2003-01-02

申请人： Isao KARUBE

发明人： Isao Karube ,  Shinya Sawata ,  Ryohei Nagata

IPC分类号： C12Q001/68 ,  C12P019/34 ,  C12M001/34

CPC分类号： C12Q1/6825 ,  C12Q1/6813 ,  Y02A50/52 ,  C12Q2565/627 ,  C12Q2525/107

摘要： An object of the present invention is to provide a method for detecting a target nucleotide sequence using the hybridization method, which has a remarkably improved sensitivity of detection. The method comprises the steps of hybridizing a target nucleotide sequence with a PNA that is complementary to the whole or a part of the target nucleotide sequence and measuring the degree of hybridization at the presence of a denaturing agent.

摘要翻译： 本发明的目的是提供一种使用杂交方法检测靶核苷酸序列的方法，其具有显着提高的检测灵敏度。 该方法包括以下步骤：将目标核苷酸序列与与靶核苷酸序列的全部或部分互补的PNA杂交并测定变性剂存在下的杂交程度。

公开(公告)号：US06500679B2

公开(公告)日：2002-12-31

申请号：US10090518

申请日：2002-03-04

申请人： Takuo Akimoto ,  Takeo Tanaami ,  Kazunori Ikebukuro ,  Kazuyoshi Yano ,  Isao Karube

发明人： Takuo Akimoto ,  Takeo Tanaami ,  Kazunori Ikebukuro ,  Kazuyoshi Yano ,  Isao Karube

IPC分类号： H01L2100

CPC分类号： G02B1/10 ,  G01N21/6452

摘要： The present invention aims to achieve a chip having a construction in which each film of metal, dielectric, and fluorescent material is stacked on a glass substrate 1. Such a construction can enhance the intensity of fluorescence emitted from a fluorescent material owing to the interaction between the fluorescent material and the light propagation mode in that construction.

公开(公告)号：US07964077B2

公开(公告)日：2011-06-21

申请号：US11510838

申请日：2006-08-28

申请人： Yuji Maruo ,  Katsuyoshi Takahashi ,  Yutaka Unuma ,  Keisuke Usui ,  Hideki Kinoshita ,  Kisho Shiseki ,  Yoshio Suzuki ,  Atsunori Hiratsuka ,  Hiroyuki Fukui ,  Kenji Yokoyama ,  Ichiji Namatame ,  Kouhei Yodoya ,  Yasuhiro Ogawa ,  Koji Sakairi ,  Chie Hayashida ,  Satonari Akutsu ,  Kazuyoshi Yano ,  Isao Karube

发明人： Yuji Maruo ,  Katsuyoshi Takahashi ,  Yutaka Unuma ,  Keisuke Usui ,  Hideki Kinoshita ,  Kisho Shiseki ,  Yoshio Suzuki ,  Atsunori Hiratsuka ,  Hiroyuki Fukui ,  Kenji Yokoyama ,  Ichiji Namatame ,  Kouhei Yodoya ,  Yasuhiro Ogawa ,  Koji Sakairi ,  Chie Hayashida ,  Satonari Akutsu ,  Kazuyoshi Yano ,  Isao Karube

IPC分类号： G01N27/26 ,  G01N27/447

CPC分类号： G01N27/44778

摘要： The present invention is to provide a sample separation instrument used in a sample separation apparatus which includes: holding means for holding a first medium supporter which supports a first medium; and driving means for moving fixing means or the holding means in a direction parallel or perpendicular to a plane whose sides extend in the first direction and in the second directions. The sample separation instrument includes an insulator for storing a second medium which allows a sample separated in the first medium in the first direction to be further separated in the second direction different from the first direction, wherein: the insulator includes a first opening and a second opening each of which defines the second direction in which the second medium is electrified, and the second opening has a shape which allows the first medium containing the separated sample to be attached to the second medium along the first direction. The present invention realizes automation of the two-dimensional electrophoresis to enhance the convenience of the two-dimensional electrophoresis, to less depend on the operator's skill, and to enhance the reproducibility of the electrophoresis result.

摘要翻译： 本发明提供一种在样品分离装置中使用的样品分离装置，其包括：保持装置，用于保持支撑第一介质的第一介质支持体; 以及用于在平行或垂直于其侧面沿第一方向和第二方向延伸的平面的方向上移动固定装置或保持装置的驱动装置。 样品分离装置包括用于存储第二介质的绝缘体，其允许在第一方向上在第一介质中分离的样品在与第一方向不同的第二方向上进一步分离，其中：绝缘体包括第一开口和第二开口 其中每个开口限定第二介质带电的第二方向，第二开口具有允许包含分离样品的第一介质沿着第一方向附着到第二介质的形状。 本发明实现了二维电泳的自动化，以增强二维电泳的便利性，较少依赖于操作者的技能，并提高电泳结果的再现性。

公开(公告)号：US20090123335A1

公开(公告)日：2009-05-14

申请号：US11885945

申请日：2006-10-25

申请人： Hideaki Nakamura ,  Masao Gotoh ,  Tomoko Ishikawa ,  Isao Karube ,  Toshifumi Hosoya ,  Shingo Kaimori ,  Moriyasu Ichino

发明人： Hideaki Nakamura ,  Masao Gotoh ,  Tomoko Ishikawa ,  Isao Karube ,  Toshifumi Hosoya ,  Shingo Kaimori ,  Moriyasu Ichino

IPC分类号： G01N31/22

CPC分类号： C12Q1/001 ,  G01N27/3272 ,  G01N33/5438

摘要： It is intended to provide a sensor chip that enables detection of a deterioration state of a reagent applied on a reaction portion when measuring a concentration of a measurement object substance and a sensor system that enables a correct measurement by using the sensor chip for detecting the reagent deterioration state. A sensor chip including two substrates opposed to each other, a spacer layer sandwiched between the substrates, a multiple reaction portions provided in the spacer layer, and detection electrode units disposed on surfaces of the substrates facing to the spacer layer and exposed to the reaction portions, wherein an identical reagent A is applied on two or more of the reaction portions, and another reagent B that reacts with the reagent A is applied on at least one of the two reaction portions and a sensor system including the sensor chip and a measurement unit for comparing current values from the two or more reaction portions on which the identical reagent A is applied.

摘要翻译： 本发明旨在提供一种传感器芯片，其能够检测在测量对象物质的浓度的反应部分上施加的试剂的劣化状态，以及能够通过使用用于检测试剂的传感器芯片进行正确测量的传感器系统 劣化状态。 一种传感器芯片，包括彼此相对的两个基板，夹在基板之间的间隔层，设置在间隔层中的多个反作用部分和设置在面向间隔层的基板的表面上并暴露于反应部分的检测电极单元 其中相同的试剂A施加在两个或更多个反应部分上，另一个与试剂A反应的试剂B施加在两个反应部分中的至少一个上，以及包括传感器芯片和测量单元的传感器系统 用于比较来自施加相同试剂A的两个或更多个反应部分的电流值。