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    Expression monitoring by hybridization to high density oligonucleotide arrays
    21.
    发明授权
    Expression monitoring by hybridization to high density oligonucleotide arrays 失效
    通过与高密度寡核苷酸阵列杂交进行表达监测

    公开(公告)号：US06927032B2

    公开(公告)日：2005-08-09

    申请号：US10353792

    申请日：2003-01-28

    Applicant: David J. Lockhart ,  Eugene L. Brown ,  Gordon G. Wong ,  Mark Chee ,  Thomas R. Gingeras

    Inventor： David J. Lockhart ,  Eugene L. Brown ,  Gordon G. Wong ,  Mark Chee ,  Thomas R. Gingeras

    IPC: G01N33/53 ,  C12N15/09 ,  C12Q1/68 ,  G01N15/14 ,  G01N37/00 ,  C07H21/02 ,  C07H21/04 ,  C12M1/34 ,  C12P19/34

    CPC classification number: C12Q1/6809 ,  C12Q1/6837 ,  G01N15/1475 ,  C12Q2565/507

    Abstract: This invention provides methods of monitoring the expression levels of a multiplicity of genes. The methods involve hybridizing a nucleic acid sample to a high density array of oligonucleotide probes where the high density array contains oligonucleotide probes complementary to subsequences of target nucleic acids in the nucleic acid sample. In one embodiment, the method involves providing a pool of target nucleic acids comprising RNA transcripts of one or more target genes, or nucleic acids derived from the RNA transcripts, hybridizing said pool of nucleic acids to an array of oligonucleotide probes immobilized on surface, where the array comprising more than 100 different oligonucleotides and each different oligonucleotide is localized in a predetermined region of the surface, the density of the different oligonucleotides is greater than about 60 different oligonucleotides per 1 cm2, and the olignucleotide probes are complementary to the RNA transcripts or nucleic acids derived from the RNA transcripts; and quantifying the hybridized nucleic acids in the array.

    Abstract translation: 本发明提供监测多种基因的表达水平的方法。 所述方法包括将核酸样品与寡核苷酸探针的高密度阵列杂交，其中高密度阵列含有与核酸样品中靶核酸的子序列互补的寡核苷酸探针。 在一个实施方案中，所述方法包括提供包含一个或多个靶基因的RNA转录物或衍生自RNA转录物的核酸的靶核酸库，将所述核酸库与固定在表面上的寡核苷酸探针阵列杂交，其中 包含超过100种不同寡核苷酸的阵列和每种不同的寡核苷酸被定位在表面的预定区域中，不同寡核苷酸的密度大于每1cm 2大约60个不同的寡核苷酸，并且寡核苷酸 探针与衍生自RNA转录物的RNA转录物或核酸互补; 并定量阵列中的杂交核酸。

    Methods of using an array of pooled probes in genetic analysis
    25.
    发明授权
    Methods of using an array of pooled probes in genetic analysis 有权
    在遗传分析中使用汇集探针阵列的方法

    公开(公告)号：US06306643B1

    公开(公告)日：2001-10-23

    申请号：US09138958

    申请日：1998-08-24

    Applicant: Erik Gentalen ,  Mark Chee

    Inventor： Erik Gentalen ,  Mark Chee

    IPC: C12M134

    CPC classification number: C12Q1/6837 ,  C12Q1/6827 ,  C12Q1/6874 ,  C12Q2565/543 ,  C12Q2565/513

    Abstract: The invention provides arrays of polynucleotide probes having at least one pooled position. A typical array comprises a support having at least three discrete regions. A first region bears a pool of polynucleotide probes comprising first and second probes. A second region bears the first probe without the second probe and a third region bears the second probe without the first probe. A target nucleic acid having segments complementary to both the first and second probes shows stronger normalized binding to the first region than to the aggregate of binding to the second and third regions due to cooperative binding of pooled probes in the first region. The invention provide methods of using such arrays for e.g., linkage analysis, sequence analysis, and expression monitoring.

    Abstract translation: 本发明提供具有至少一个合并位置的多核苷酸探针阵列。 典型的阵列包括具有至少三个离散区域的支撑件。 第一区域具有包含第一和第二探针的多核苷酸探针池。 第二区域承载第一探针而没有第二探针，第三区域承载第二探针而没有第一探针。 具有与第一和第二探针两者互补的区段的靶核酸由于在第一区域中的合并探针的协同结合而显示比第一区域更强的标准化结合，而不是与第二和第三区域结合的聚集体。 本发明提供了使用这种阵列进行例如连锁分析，序列分析和表达监测的方法。

    Polymorphisms in the glucose-6 phosphate dehydrogenase locus
    28.
    发明授权
    Polymorphisms in the glucose-6 phosphate dehydrogenase locus 失效
    葡萄糖-6-磷酸脱氢酶基因座的多态性

    公开(公告)号：US5856104A

    公开(公告)日：1999-01-05

    申请号：US813508

    申请日：1997-03-07

    Applicant: Mark Chee ,  Jian-Bing Fan

    Inventor： Mark Chee ,  Jian-Bing Fan

    IPC: C12Q1/68 ,  C07H21/04

    CPC classification number: C12Q1/6827 ,  C12Q1/6876 ,  C12Q2600/156

    Abstract: The invention provides nucleic acid segments of the glucose-6 phosphate dehydrogenase locus of the human genome including polymorphic sites. Allele-specific primers and probes hybridizing to regions flanking these sites are also provided. The nucleic acids, primers and probes are used in applications such as forensics, paternity testing, medicine and genetic analysis.

    Abstract translation: 本发明提供人类基因组的葡萄糖-6-磷酸脱氢酶基因座的核酸片段，包括多态位点。 还提供了与这些位点侧翼区域杂交的等位基因特异性引物和探针。 核酸，引物和探针用于取证，亲子鉴定，药物和遗传分析等应用。

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