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    COMPOSITIONS AND METHODS FOR REPRESENTATIONAL SELECTION OF NUCLEIC ACIDS FROM COMPLEX MIXTURES USING HYBRIDIZATION
    3.
    发明申请
    COMPOSITIONS AND METHODS FOR REPRESENTATIONAL SELECTION OF NUCLEIC ACIDS FROM COMPLEX MIXTURES USING HYBRIDIZATION 有权
    使用混合从复合混合物中代表性选择核酸的组合物和方法

    公开(公告)号：US20100093550A1

    公开(公告)日：2010-04-15

    申请号：US12445069

    申请日：2007-08-24

    申请人： John R. Stuelpnagel ,  David L. Barker ,  Jorge Velarde, JR. ,  Steven M. Barnard ,  Michael Graige

    发明人： John R. Stuelpnagel ,  David L. Barker ,  Jorge Velarde, JR. ,  Steven M. Barnard ,  Michael Graige

    IPC分类号： C40B20/02

    CPC分类号： C12N15/1072 ,  C12Q1/6837 ,  C12Q2565/515

    摘要： The invention provides a method of selecting a representational sample of nucleic acid sequences from a complex mixture. The method includes: (a) contacting a complex mixture of nucleic acids under conditions sufficient for hybridization with a population of capture probes complementary to one or more nucleic acids comprising a predetermined portion of the sequence collectively present in the complex mixture to form hybridization complexes of the one or more nucleic acids with the population of probes, the population of capture probes being attached to a solid support, and (b) removing unhybridized nucleic acids to select a representational sample of nucleic acids having a complexity of less than 10% but more than 0.001% of the complex mixture, wherein the representational sample comprises a nucleic acid copy having a proportion of each sequence in the copy relative to all other sequences in the copy substantially the same as the proportions of the sequences in the predetermined portion of one or more nucleic acids within the complex mixture. A method of selecting a representational sample of genomic sequences from a complete genome also is provided. The invention further provides a nucleic acid population that includes a representational sample having a complexity of less than 10% but more than 0.001% of a complex mixture, the representational sample comprising a nucleic acid copy having a proportion of each sequence in the copy relative to all other sequences in the copy substantially the same as the proportions of sequences in a predetermined portion of a sequence collectively present in one or more nucleic acids within the complex mixture.

    摘要翻译： 本发明提供了从复杂混合物中选择核酸序列的表示样品的方法。 该方法包括：（a）在足以与包含与复合物混合物中共同存在的序列的预定部分的一个或多个核酸互补的捕获探针的杂交群的条件下使核酸的复杂混合物接触，以形成杂合复合物 具有探针群的一个或多个核酸，捕获探针的群体连接到固体支持物上，和（b）除去未杂交的核酸以选择复杂度小于10％但更多的核酸的代表性样品 超过0.001％的复合混合物，其中代表性样品包含核酸拷贝，其拷贝中的每个序列的比例相对于拷贝中的所有其它序列基本上与在一个或更多个预定部分中的序列的比例相同 复杂混合物中更多的核酸。 还提供了从完整基因组中选择基因组序列的表示样品的方法。 本发明还提供了一种核酸群体，其包括具有小于复合物混合物的10％但大于0.001％的复杂性的代表性样品，所述代表性样品包含核酸拷贝，所述核酸拷贝具有拷贝中每个序列的比例相对于 拷贝中的所有其它序列基本上与复合物混合物中一个或多个核酸中共存的序列的预定部分中的序列比例相同。

    Method and apparatus for retaining and presenting at least one microsphere array to solutions and/or to optical imaging systems
    7.
    发明授权
    Method and apparatus for retaining and presenting at least one microsphere array to solutions and/or to optical imaging systems 有权
    用于将至少一个微球阵列保持并呈现给溶液和/或光学成像系统的方法和装置

    公开(公告)号：US06396995B1

    公开(公告)日：2002-05-28

    申请号：US09574962

    申请日：2000-05-19

    申请人： John R. Stuelpnagel ,  Mark S. Chee ,  Richard J. Pytelewski ,  Todd Alan Dickinson ,  Gan G. Wang

    发明人： John R. Stuelpnagel ,  Mark S. Chee ,  Richard J. Pytelewski ,  Todd Alan Dickinson ,  Gan G. Wang

    IPC分类号： G02B600

    CPC分类号： G01N35/028 ,  B01J2219/00317 ,  B01J2219/00585 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00707 ,  B01L2300/0893 ,  C40B60/14 ,  G01N21/253 ,  G01N21/645 ,  G01N21/6452

    摘要： A holder for an array of bundles containing multiple fiber optic strands provides a pattern of openings into which one each of each bundle is fit. The openings may (but need not) penetrate the full holder thickness to permit working with bundles from either bundle end. In one embodiment the holder thickness equals the bundle length, and the holder is sized similarly to a microscope slide. An alternative embodiment temporarily retains bundles in a desired registration and subjects the retained bundles to molten material such as wax that is allowed to harden around the bundles and become the holder. A modular holder comprises laminates that each contain parallel grooves sized to receive a bundle. A holder for a single bundle includes cooperating grip-like members, one of which is pivotably attached and biased toward the other member. A common form factor may be used for each holder type to facilitate retaining holders in a common docking station.

    摘要翻译： 用于包含多个光纤线束的束阵列的保持器提供开口的图案，每个束中的每一个都适合于其中。 开口可以（但不需要）穿透整个夹持器的厚度，以允许从任一个束末端束起束。 在一个实施例中，保持器厚度等于束长度，并且保持器的尺寸与显微镜载玻片类似。 一个替代实施例暂时将束保持在期望的配准中，并将保留的束经受允许在束附近硬化并变成保持器的熔融材料例如蜡。 模块化夹持器包括每​​个包含尺寸适于接收束的平行凹槽的层压板。 用于单个束的保持器包括配合的手柄状构件，其中一个可枢转地附接并朝向另一个构件偏置。 每个保持器类型可以使用常见的形状因子，以便于将保持器固定在公共坞站中。