中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

155 records (took 0.038 seconds)

    Optimization of gene expression analysis using immobilized capture probes
    23.
    发明授权
    Optimization of gene expression analysis using immobilized capture probes 有权
    使用固定化捕获探针优化基因表达分析

    公开(公告)号：US08795960B2

    公开(公告)日：2014-08-05

    申请号：US12480215

    申请日：2009-06-08

    Applicant: Michael Seul ,  Sukanta Banerjee ,  Jiacheng Yang ,  Tatiana Vener

    Inventor： Michael Seul ,  Sukanta Banerjee ,  Jiacheng Yang ,  Tatiana Vener

    IPC: C12Q1/68 ,  C12P19/34 ,  C12M1/34 ,  C12M3/00 ,  C07H21/04

    CPC classification number: C12Q1/6832 ,  B01J2219/00459 ,  B01J2219/00576 ,  B01J2219/00608 ,  B01J2219/00648 ,  B01J2219/00722 ,  C12Q1/6809 ,  C12Q1/6834 ,  C12Q1/6837 ,  C12Q2537/143 ,  C12Q2565/501 ,  C12Q2565/507 ,  C12Q2533/101 ,  C12Q2565/514 ,  C12Q2565/519

    Abstract: Disclosed are methods of multiplexed analysis of oligonucleotides in a sample, including: methods of probe and target “engineering”, as well as methods of assay signal analysis relating to the modulation of the probe-target affinity constant, K by a variety of factors including the elastic properties of target strands and layers of immobilized (“grafted”) probes; and assay methodologies relating to: the tuning of assay signal intensities including dynamic range compression and on-chip signal amplification; the combination of hybridization-mediated and elongation-mediated detection for the quantitative determination of abundance of messages displaying a high degree of sequence similarity, including, for example, the simultaneous determination of the relative expression levels, and identification of the specific class of, untranslated AU-rich subsequences located near the 3′ terminus of mRNA; and a new method of subtractive differential gene expression analysis which requires only a single color label.

    Abstract translation: 公开了样品中寡核苷酸多重分析的方法，包括：探针和靶标“工程”的方法，以及通过多种因素与探针 - 靶亲和常数K的调节相关的信号分析方法，包括 目标链和固定（“接枝”）探针层的弹性性质; 以及与以下方法相关的测定方法：调整测定信号强度，包括动态范围压缩和片上信号放大; 杂交介导和延长介导检测的组合用于定量测定显示高度序列相似性的消息丰度，包括例如同时确定相对表达水平，以及鉴定特异性类别，未翻译 位于mRNA的3'末端附近的富含子序列; 以及仅需要单一颜色标签的减法差分基因表达分析的新方法。

    Method and apparatus for fulfilling requests for perishable items
    24.
    发明授权
    Method and apparatus for fulfilling requests for perishable items 有权
    满足易腐物品要求的方法和装置

    公开(公告)号：US08504388B2

    公开(公告)日：2013-08-06

    申请号：US13366702

    申请日：2012-02-06

    Applicant: Michael Seul ,  Andreas E. Gocksch

    Inventor： Michael Seul ,  Andreas E. Gocksch

    IPC: G06Q10/00 ,  G06Q50/00 ,  G06Q30/00

    CPC classification number: G06Q50/22 ,  G06Q10/06315 ,  G06Q10/087

    Abstract: Apparatuses, methods, and computer readable medium, for fulfilling a need for at least one perishable item, the method including reserving a plurality of perishable items with at least partially unknown attribute profiles from a supplier; receiving values for at least some of the reserved at least partially unknown attribute profiles, wherein the received values are determined by tests conducted after the reserving step; determining based on the received values which of the plurality of perishable items satisfy the need for the at least one perishable item; and if at least one of the plurality of perishable items does not satisfy the need for the at least one perishable item, unreserving the at least one perishable item of the plurality of perishable items determined not to satisfy the need for the at least one perishable item.

    Abstract translation: 用于满足至少一个易腐物品的需要的装置，方法和计算机可读介质，所述方法包括从供应商保留具有至少部分未知属性简档的多个易腐物品; 接收所述保留的至少部分未知属性简档中的至少一些的值，其中通过在所述保留步骤之后进行的测试来确定所接收的值; 基于所接收的值确定所述多个易腐品中的哪一个满足对所述至少一个易腐物品的需要; 并且如果所述多个易腐物品中的至少一个不满足对所述至少一个易腐物品的需要，则不保留所述多个易腐物品中的至少一个易腐物品被确定为不满足所述至少一个易腐物品的需要 。

    Creation of functionalized microparticle libraries by oligonucleotide ligation or elongation
    25.
    发明授权
    Creation of functionalized microparticle libraries by oligonucleotide ligation or elongation 有权
    通过寡核苷酸连接或延伸产生官能化微粒文库

    公开(公告)号：US08486629B2

    公开(公告)日：2013-07-16

    申请号：US11411510

    申请日：2006-04-26

    Applicant: Sukanta Banerjee ,  Jiacheng Yang ,  Michael Seul

    Inventor： Sukanta Banerjee ,  Jiacheng Yang ,  Michael Seul

    IPC: C12Q1/68 ,  C40B50/00

    CPC classification number: B01J19/0046 ,  B01J2219/005 ,  B01J2219/00545 ,  B01J2219/00576 ,  B01J2219/00648 ,  B01J2219/00722 ,  B01J2219/00725 ,  C12N15/1093 ,  C40B20/04 ,  C40B50/14

    Abstract: Disclosed are methods of for constructing a bead-displayed library of oligonucleotide probes (or sequence-modified capture moieties such as protein-nucleic acid conjugates) by ligation of a capture probe, having an analyte-specific sequence, to an anchor probe that is attached, at its 5′-end, (or possibly at the 3′ end) to an encoded carrier such as a color-coded microparticle (“bead”). Such a library can also be constructed by elongation of an anchor probe, using a second probe as the elongation template, wherein the second probe has an anchor-specific subsequence and an analyte-specific subsequence.

    Abstract translation: 公开了通过将具有分析物特异性序列的捕获探针连接到所连接的锚定探针上来构建寡核苷酸探针（或序列修饰的捕获部分，例如蛋白质 - 核酸缀合物）的珠显示文库的方法 （或可能在3'端）的编码载体，例如着色微粒（“珠粒”）。 也可以使用第二探针作为延伸模板，通过锚定探针的伸长来构建这种文库，其中第二探针具有锚特异性亚序列和分析物特异性亚序列。

    AUTOMATED ANALYSIS OF MULTIPLEXED PROBE-TARGET INTERACTION PATTERNS: PATTERN MATCHING AND ALLELE IDENTIFICATION
    26.
    发明申请
    AUTOMATED ANALYSIS OF MULTIPLEXED PROBE-TARGET INTERACTION PATTERNS: PATTERN MATCHING AND ALLELE IDENTIFICATION 有权
    多重探测目标相互作用模式的自动分析：模式匹配与识别

    公开(公告)号：US20120065099A1

    公开(公告)日：2012-03-15

    申请号：US13297487

    申请日：2011-11-16

    Applicant: Xiongwu Xia ,  Michael Seul

    Inventor： Xiongwu Xia ,  Michael Seul

    IPC: C40B30/04

    CPC classification number: C12Q1/6827 ,  G06F19/18 ,  G06F19/22

    Abstract: Methods and algorithms for automated allele assignments within an integrated software environment are provided. These methods and algorithms offer a multiplicity of functionalities including: data management; system configuration including user authorization, training set analysis and probe masking; pattern analysis including string matching and probe flipping; and interactive redaction of data. The methods and algorithms further include methods of setting thresholds, refining thresholds, and probe masking of signals produced by probes which do not contribute significantly to discriminating among alleles.

    Abstract translation: 提供了集成软件环境中自动等位基因分配的方法和算法。 这些方法和算法提供了多种功能，包括：数据管理; 系统配置包括用户授权，训练集分析和探测屏蔽; 模式分析包括字符串匹配和探针翻转; 和数据的交互式修改。 所述方法和算法还包括设置阈值，精确阈值和由探针产生的信号的探针掩蔽的方法，这些信号对于等位基因之间的区分不起重要作用。

    Number coding for identification of subtypes of coded types of solid phase carriers
    29.
    发明授权
    Number coding for identification of subtypes of coded types of solid phase carriers 有权
    用于识别编码类型的固相载体亚型的编号

    公开(公告)号：US07927796B2

    公开(公告)日：2011-04-19

    申请号：US10943760

    申请日：2004-09-17

    Applicant: Michael Seul ,  Enqing Tan ,  Chiu Chau

    Inventor： Michael Seul ,  Enqing Tan ,  Chiu Chau

    IPC: C12Q1/68 ,  C12P19/34 ,  C07H21/04 ,  G01N33/543 ,  G01N15/06 ,  G01N33/48

    CPC classification number: G01N33/6845 ,  G01N33/543 ,  G01N33/54313 ,  G01N33/564

    Abstract: Disclosed is number coding of pairs (“doublets”) or small sets (“multiplets”) of solid phase carriers which provides distinguishable subtypes of a given type of such carriers, where each carrier type is distinguishable on the basis of a different code. Such number coding is useful for augmenting a coding system, such as a color code, and thereby effectively multiplying the number of “colors” (distinguishable sub-types). It can be applied, for example, to determine whether a sample is homozygous or heterozygous at a number of different sites for one of two different alleles, where the same color code is applied for each of the two alleles, and the alleles with the same color code are distinguished by knowing how many carriers are associated with molecules which detect each different allele.

    Abstract translation: 披露了提供给定类型的这种载体的可区分子类型的固相载体的对（“双”）或小集（“多重”）的数字编码，其中每种载体类型基于不同的代码可区分。 这种数字编码对于增加诸如颜色代码的编码系统是有用的，从而有效地乘以“颜色”的数量（可区分的子类型）。 例如，它可以应用于确定样品在两个不同等位基因之一的多个不同位点是纯合的还是杂合的，其中针对两个等位基因中的每一个应用相同的颜色代码，并且具有相同的等位基因 通过知道多少载体与检测每​​个不同等位基因的分子相关联来区分颜色代码。

Patent Agency Ranking