公开(公告)号：US20210236771A1

公开(公告)日：2021-08-05

申请号：US17048862

申请日：2019-04-19

Applicant: Wake Forest University Health Sciences

Inventor： James D. Turner ,  Sean Dobson

IPC: A61M25/01 ,  A61B34/20 ,  A61B8/08

Abstract: A medical device is provided that can be held and operated with a single hand to cause a catheter to be placed at a selected location in a patient's body. The user's other hand is free to perform other tasks, such as operating an ultrasound probe, for example. The medical device may also include a valve that can be operated with the same hand that holds the medical device to open a port to cause a fluid, such as a nerve block agent, for example, to be injected into the patient's body at the selected location.

公开(公告)号：US11046823B2

公开(公告)日：2021-06-29

申请号：US16322219

申请日：2017-08-02

Applicant: WAKE FOREST UNIVERSITY HEALTH SCIENCES

Inventor： Carlos Kengla

IPC: C08J3/205 ,  A61L27/46 ,  C09D11/10 ,  A61P19/08 ,  A61L27/56 ,  B29C64/118 ,  B29C64/209 ,  C08J3/12 ,  C08K3/32 ,  B33Y10/00 ,  B33Y70/00 ,  B33Y80/00 ,  B29L31/00 ,  C08J9/00

Abstract: Provided herein are improved compositions and methods of making and using the same, the composition comprising a polymer and a ceramic present at a ratio of from 3:1 to 1:3 of polymer:ceramic by weight, wherein the composition comprises or is a composite of the polymer and the ceramic having improved printability and/or having an improved elastic modulus and/or improved stress at failure (e.g., as compared to a blend of the polymer and the ceramic).

公开(公告)号：US11033430B2

公开(公告)日：2021-06-15

申请号：US16050003

申请日：2018-07-31

Applicant: Wake Forest University Health Sciences

Inventor： Keith Andrew Walter ,  Evan Scott Luxon ,  Christopher Bligh Komanski

IPC: A61F9/008 ,  A61B34/00 ,  A61B34/10

Abstract: Methods and systems for correcting presbyopia using a surgical excimer laser include activating the laser once and transmitting a pre-defined three dimensional ablation profile to treat presbyopia based on the single activating step.

公开(公告)号：US20210162017A1

公开(公告)日：2021-06-03

申请号：US17105156

申请日：2020-11-25

Applicant: Wake Forest University Health Sciences

Inventor： James YOO ,  Sang Jin Lee ,  Anthony Atala ,  Mark Van Dyke

IPC: A61K38/39 ,  A61K9/00 ,  A61K38/48 ,  A61L27/24 ,  A61L27/52 ,  A61L27/54

Abstract: The invention is directed to methods of inducing cell recruitment and tissue regeneration at a target site in a subject. It is also based, in part, on the discovery that a subject's own biologic resources and environmental conditions can be used for in situ tissue regeneration and thereby reduce or eliminate the need for donor cell procurement and ex vivo manipulation of such donor cells. Methods are disclosed for recruitment of a subject's own stem cells to a target region by inducing a sustained positive pressure at a target site, such as the kidney, thereby increasing the number of pluripotent cells capable of differentiating to regenerate the target tissue.

公开(公告)号：US11013825B2

公开(公告)日：2021-05-25

申请号：US15672885

申请日：2017-08-09

Applicant: Wake Forest University Health Sciences

Inventor： Hyun-Wook Kang ,  Sang Jin Lee ,  Anthony Atala ,  James J. Yoo

IPC: B29C64/112 ,  A61L27/38 ,  A61L27/20 ,  B29C64/393 ,  A61L27/22 ,  A61L27/52 ,  B29C64/124 ,  A61L27/26 ,  C12M3/00 ,  C12M1/00 ,  C12M1/26 ,  A61K35/12 ,  B33Y10/00 ,  B33Y30/00 ,  B33Y70/00 ,  B33Y80/00 ,  B29K105/00 ,  B29K267/00 ,  B29L31/00

Abstract: A method of making an organ or tissue comprises: (a) providing a first dispenser containing a structural support polymer and a second dispenser containing a live cell-containing composition; (b) depositing a layer on said support from said first and second dispenser, said layer comprising a structural support polymer and said cell-containing composition; and then (c) iteratively repeating said depositing step a plurality of times to form a plurality of layers one on another, with separate and discrete regions in each of said layers comprising one or the other of said support polymer or said cell-containing composition, to thereby produce provide a composite three dimensional structure containing both structural support regions and cell-containing regions. Apparatus for carrying out the method and composite products produced by the method are also described.

公开(公告)号：US20210047375A1

公开(公告)日：2021-02-18

申请号：US17051296

申请日：2019-05-01

Applicant: Wake Forest University Health Sciences

Inventor： Baisong Lu ,  Anthony Atala

IPC: C07K14/155 ,  C12N9/22 ,  C12N15/86 ,  C12N15/113

Abstract: Provided are compositions, systems, and methods useful for effecting gene editing in eukaryotic cells. Compositions include plasmids that encode one or more viral fusion proteins in which one or more viral proteins are fused with an aptamer-binding protein. Compositions also include plasmids that encode a non-viral nucleic acid sequence, wherein the non-viral nucleic acid sequence encodes a CRISPR system component. In some instances, the non-viral nucleic acid sequence also includes an aptamer sequence. The plasmids can be used to generate viral particles, including lentivirus-like particles that contain a viral fusion protein and a non-viral RNA sequence. Systems of producing such viral particles are provided. Also provided are methods of using the viral particles of the disclosure to effect gene editing in eukaryotic cells.

公开(公告)号：US20200376489A1

公开(公告)日：2020-12-03

申请号：US16635703

申请日：2018-08-01

Applicant: Wake Forest University Health Sciences

Inventor： Christopher Daniel PORADA ,  Aleksander SKARDAL ,  Maria Graca ALMEIDA-PORADA

IPC: B01L3/00 ,  C12M3/00 ,  C12M1/00

Abstract: Provided herein according to some embodiments is an in vitro construct useful as a model for a hematopoietic microenvironment, which may include: a microfluidic device having multiple chambers; and two or more populations of cells (e.g., 3 or 4 populations of cells) (or “niches”) selected from: 1) mesenchymal cells (e.g., Stro-1+; MSC); 2) osteoblasts (OB; optionally said osteoblasts provided by differentiating mesenchymal cells to differentiated osteoblasts); 3) arterial endothelium (e.g., CD146+NG2+; AEC); and 4) sinusoidal endothelium (CD146+NG2−; SEC), wherein each of said two or more populations of cells are provided in a separate chamber of the microfluidic device. Methods of making and using the construct are also provided.

公开(公告)号：US10821211B2

公开(公告)日：2020-11-03

申请号：US16227556

申请日：2018-12-20

Applicant: Wake Forest University Health Sciences

Inventor： Mark E. Van Dyke

IPC: A61L33/12 ,  A61L15/32 ,  A61L26/00 ,  A61L27/22 ,  A61L27/34 ,  A61L29/04 ,  A61L29/08 ,  A61L31/04 ,  A61L31/10 ,  A61K38/17 ,  A61L27/54 ,  A61L29/16 ,  A61L31/16 ,  A61L33/00 ,  C07K1/36 ,  C07K14/47

Abstract: Methods are provided to produce optimal fractionations of charged keratins that have superior biomedical activity. Also provided are medical implants coated with these keratin preparations. Further provided are methods of treating blood coagulation in a patient in need thereof.

公开(公告)号：US10751448B2

公开(公告)日：2020-08-25

申请号：US15542095

申请日：2016-01-11

Applicant: Wake Forest University Health Sciences

Inventor： Anthony Atala ,  Gayoung Jeong ,  James J. Yoo ,  Sang jin Lee ,  Young-Joon Seol

IPC: A61L27/60 ,  A61F2/10 ,  B33Y80/00 ,  B33Y70/00 ,  C08L5/08 ,  A61L27/20 ,  C08L89/06 ,  C12N5/071 ,  B33Y10/00 ,  B29C64/106 ,  A61L27/18 ,  A61L27/38 ,  A61L27/52 ,  G01N33/50 ,  B29K75/00 ,  B29K105/00

Abstract: Provided are live, artificial, skin substitute products and methods of making and using the same, such as for wound treatment and compound testing, including compound testing for efficacy, toxicity, penetration, irritation and/or metabolism testing of drug candidates or compositions such as cosmetics. Described herein is an artificial mammalian skin substitute product, comprising: (a) optionally, but in some embodiments preferably, a first (“hypodermis-like”) layer comprising live mammalian adipocytes (e.g., induced pre-adipocytes) in a first hydrogel carrier; (b) a second (“dermis-like”) layer contacting or directly contacting the first layer and comprising live mammalian fibroblast cells and' live mammalian follicle dermal papilla cells in combination in a second hydrogel carrier; (c) a third (“epidermis-like”) layer contacting or directly contacting the second layer (i.e., on the opposite side thereof as the first layer, so that the second layer is sandwiched between the first and third layers when the first layer is present), the third layer comprising live mammalian keratinocytes and live mammalian melanocytes in combination in a third hydrogel carrier.

公开(公告)号：US20200085579A1

公开(公告)日：2020-03-19

申请号：US16618926

申请日：2018-06-12

Applicant: WAKE FOREST UNIVERSITY HEALTH SCIENCES

Inventor： Phillip Kim ,  Anthony Atala ,  David James Mccoul

IPC: A61F2/24 ,  G16H50/50 ,  G16H30/40 ,  A61B34/10

Abstract: Provided herein is a method of forming a ventricular support device for a diseased heart, including providing imaging data of the diseased heart, forming a three-dimensional (3D) heart model based on the imaging data, providing strain data including a plurality of strain estimates for at least one segment of the diseased heart, mapping the plurality of strain estimates onto corresponding portions of the 3D heart model to form a 3D diseased heart model, based on the 3D diseased heart model, forming a model of the ventricular support device configured to surround at least a portion of the diseased heart and provide support based upon said strain estimates, and converting the model of the ventricular support device to a digital file useful for directing a 3D printer device to print said ventricular support device for said diseased heart. Also provided is a customized ventricular support device so produced.