公开(公告)号：US20220135957A1

公开(公告)日：2022-05-05

申请号：US17349933

申请日：2021-06-17

申请人： Jing Wu ,  Lingqia Su ,  Yan Huang

发明人： Jing Wu ,  Lingqia Su ,  Yan Huang

IPC分类号： C12N9/18 ,  C12N9/92 ,  C12N9/10 ,  C12N15/75

摘要： Disclosed is a method for promoting extracellular expression of proteins in B. subtilis using cutinase, which belongs to the technical fields of genetic engineering, enzyme engineering and microbial engineering. It teaches co-expressing a cutinase mutant and a target protein in B. subtilis to promote extracellular expression of the target protein which is naturally located inside cells. The target protein includes xylose isomerase, 4,6-α-glucosyltransferase, 4-α-glucosyltransferase, trehalose synthase, branching enzyme and the like. The invention can achieve extracellular expression of intracellularly localized target protein, improve the production efficiency, reduce the production cost and simplify the subsequent extraction process.

公开(公告)号：US10508279B2

公开(公告)日：2019-12-17

申请号：US15891564

申请日：2018-02-08

申请人： Jing Wu ,  Quan Zhang ,  Liming Liu ,  Xiulai Chen ,  Jia Liu ,  Qiuling Luo

发明人： Jing Wu ,  Quan Zhang ,  Liming Liu ,  Xiulai Chen ,  Jia Liu ,  Qiuling Luo

IPC分类号： C12N1/20 ,  C12N15/70 ,  C12N9/10 ,  C12N9/90 ,  C12P19/24 ,  C12P19/26

摘要： The present invention relates to the field of biotechnology engineering. It provides a recombinant Escherichia coli that can produce fructosylated chondroitin with high yields and the method for constructing the recombinant strain. The present invention discloses a method for constructing an expression plasmid pETM6R1-RBS-glmM-GGGS-glmS that contains a glmM and a glmS gene under regulation of ribosome binding sites. The recombinant plasmid was transformed into E. coli K4 to obtain a recombinant E. coli strain ZQ33 that can produce fructosylated chondroitin up to 3.99 g·L−1 by fed-batch fermentation in a 5-L fermentor, which was increased by 108.90% compared to that of the wild type strain.

公开(公告)号：US20170005393A1

公开(公告)日：2017-01-05

申请号：US15008583

申请日：2016-01-28

申请人： Jing Wu

发明人： Jing Wu

IPC分类号： H01Q1/24 ,  H01Q23/00 ,  H01Q9/04

CPC分类号： H01Q1/243 ,  H01Q9/42

摘要： An antenna module is provided in the present disclosure. The antenna module includes a printed circuit board and a metal frame surrounding the printed circuit board. The metal frame includes a metal radiating portion, and a first feed point, a second feed point, a first ground point and a second ground point are disposed on the printed circuit board and electrically connected to the metal radiating portion. The first feed point and the second feed point are symmetrically disposed at two opposite sides of a central axis of the printed circuit board; the first ground point and the second ground point are respectively disposed corresponding to the first feed point and the second feed point. The present disclosure also provides a mobile device using the antenna module.

摘要翻译： 在本公开中提供了一种天线模块。 天线模块包括印刷电路板和围绕印刷电路板的金属框架。 金属框架包括金属辐射部分，并且第一馈电点，第二馈电点，第一接地点和第二接地点设置在印刷电路板上并电连接到金属辐射部分。 第一供电点和第二供电点对称地设置在印刷电路板的中心轴线的两个相对侧上; 第一接地点和第二接地点分别对应于第一馈电点和第二馈电点设置。 本公开还提供了使用天线模块的移动设备。

公开(公告)号：US09455497B2

公开(公告)日：2016-09-27

申请号：US14597362

申请日：2015-01-15

申请人： Jing Wu

发明人： Jing Wu

IPC分类号： H01Q1/38 ,  H01Q5/385 ,  H01Q19/00 ,  H01Q5/392

CPC分类号： H01Q5/385 ,  H01Q5/392 ,  H01Q19/005

摘要： A multi-band antenna includes a radiating antenna member, a first parasitic antenna member, and a second parasitic antenna member. The radiating antenna member includes a feeding unit, a high frequency (HF) radiating unit and a low frequency (LF) radiating unit, the HF radiating unit and the LF radiating unit extend from the feeding unit. The first parasitic antenna member includes an HF grounding part, and an HF parasitic unit extending from the HF grounding part and adjacent to the HF radiating unit. The second parasitic antenna member includes an LF grounding part, and an LF parasitic unit extending from the LF grounding part and electromagnetically coupled to the LF radiating unit. The feeding unit is arranged between the HF grounding part and the LF grounding part; the feeding unit and the HF radiating unit define a receiving slot, and the HF parasitic unit is arranged in the receiving slot.

摘要翻译： 多频带天线包括辐射天线构件，第一寄生天线构件和第二寄生天线构件。 辐射天线构件包括馈电单元，高频（HF）辐射单元和低频（LF）辐射单元，HF辐射单元和LF辐射单元从馈送单元延伸。 第一寄生天线构件包括HF接地部分和从HF接地部分延伸并且与HF辐射单元相邻的HF寄生单元。 第二寄生天线构件包括LF接地部分和从LF接地部分延伸并且电磁耦合到LF辐射单元的LF寄生单元。 馈电单元布置在HF接地部分和LF接地部分之间; 馈送单元和HF辐射单元限定接收槽，并且HF寄生单元布置在接收槽中。

公开(公告)号：US20140063921A1

公开(公告)日：2014-03-06

申请号：US13045528

申请日：2011-03-11

申请人： Xueti Tang ,  Jing Wu

发明人： Xueti Tang ,  Jing Wu

IPC分类号： G11C11/16 ,  H01L43/12

CPC分类号： G11C11/1675 ,  G11C11/16 ,  G11C11/161 ,  H01L43/12

摘要： A method and system for providing a magnetic junction residing on a substrate and usable in a magnetic device are described. The magnetic junction includes a first pinned layer, a first nonmagnetic spacer layer having a first thickness, a free layer, a second nonmagnetic spacer layer having a second thickness greater than the first thickness, and a second pinned layer. The first nonmagnetic spacer layer resides between the pinned layer and the free layer. The first pinned layer resides between the free layer and the substrate. The second nonmagnetic spacer layer is between the free layer and the second pinned layer. Further, the magnetic junction is configured such that the free layer is switchable between a plurality of stable magnetic states when a write current is passed through the magnetic junction.

摘要翻译： 描述了一种用于提供驻留在基板上并可用于磁性装置中的磁性结的方法和系统。 磁结包括第一被钉扎层，具有第一厚度的第一非磁性间隔层，具有大于第一厚度的第二厚度的自由层，第二非磁性间隔层，以及第二钉扎层。 第一非磁性间隔层位于被钉扎层和自由层之间。 第一被钉扎层位于自由层和基底之间。 第二非磁性间隔层位于自由层和第二被钉扎层之间。 此外，磁结被配置为使得当写入电流通过磁结时，自由层可在多个稳定磁状态之间切换。

公开(公告)号：US20140011239A1

公开(公告)日：2014-01-09

申请号：US13935519

申请日：2013-07-04

申请人： Jing Wu ,  Lingqia Su ,  Jian Chen

发明人： Jing Wu ,  Lingqia Su ,  Jian Chen

IPC分类号： C12N9/20 ,  C12P21/00

CPC分类号： C12N9/20 ,  C12N9/2408 ,  C12N9/2414 ,  C12N9/2434 ,  C12N9/248 ,  C12P21/00 ,  C12P21/02

摘要： The present invention provides a method of increasing extracellular secretion of secretory proteins by co-expressing the secretory proteins with a mature cutinase. Cutinase can improve the permeability of E. coli cell membrane without destroying the membrane, and thus facilitate cross-membrane transfer of the secretory proteins co-expressed in E. coli. Increased extracellular secretion of target proteins can shorten cell culture time, reduce the formation of inclusion bodies and increase production of target proteins.

摘要翻译： 本发明提供了通过用成熟角质酶共表达分泌蛋白来增加分泌蛋白的细胞外分泌的方法。 角质酶可以改善大肠杆菌细胞膜的通透性，而不破坏膜，从而促进大肠杆菌中共表达的分泌蛋白的跨膜转移。 靶蛋白的细胞外分泌增加可以缩短细胞培养时间，减少包涵体的形成并增加靶蛋白的产生。

公开(公告)号：US20130127252A1

公开(公告)日：2013-05-23

申请号：US13298828

申请日：2011-11-17

申请人： William S. Yerazunis ,  Jing Wu ,  Bingnan Wang ,  Koon Hoo Teo

发明人： William S. Yerazunis ,  Jing Wu ,  Bingnan Wang ,  Koon Hoo Teo

IPC分类号： H02J17/00 ,  H01F38/14

CPC分类号： H01F38/14 ,  H01F27/006 ,  H02J5/005 ,  H02J50/12

摘要： A system that transfers energy wirelessly includes a transmitter of the energy and a receiver of the energy. A housing made of a material that approximates properties of a perfect magnetic conductor. The housing is arranged to direct a magnetic field from the transmitter to the receiver to improve an efficiency of the energy transfer from the transmitter to the receiver.

摘要翻译： 无线传输能量的系统包括能量的发射器和能量的接收器。 由材料制成的外壳，其接近完美磁导体的特性。 壳体被布置成将来自发射器的磁场引导到接收器，以提高从发射器到接收器的能量传递的效率。

公开(公告)号：US07759382B2

公开(公告)日：2010-07-20

申请号：US10559823

申请日：2004-06-04

申请人： Jay S. Tung ,  Lee H. Latimer ,  Jing Wu ,  Albert Garofalo ,  Michael A. Pleiss ,  Darren Dressen ,  Ashley Guinn ,  Scott A. Jenkins ,  Jennifer Sealy ,  John Tucker ,  David W. G. Wone

发明人： Jay S. Tung ,  Lee H. Latimer ,  Jing Wu ,  Albert Garofalo ,  Michael A. Pleiss ,  Darren Dressen ,  Ashley Guinn ,  Scott A. Jenkins ,  Jennifer Sealy ,  John Tucker ,  David W. G. Wone

IPC分类号： A61K31/415 ,  C07D231/10

CPC分类号： C07D231/40 ,  C07D401/04 ,  C07D401/06 ,  C07D401/12 ,  C07D405/04 ,  C07D405/12 ,  C07D409/12

摘要： This invention is directed to acylated amino acid amidyl pyrazoles and related compounds of Formula I. The invention is also directed to a pharmaceutical formation comprising such compound or in a pharmaceutically acceptable salt form thereof. The invention is further directed to a method for inhibiting β-amyloid peptide release and/or synthesis, a method for inhibiting γ-secretase activity, and a method for treating neurological disorders associated with β-amyloid peptide production. The method comprises administering to a host a pharmaceutical formulation comprising an effective amount of a compound of Formula I. The compounds of Formula I are useful in the prevention and treatment of Alzheimer's disease.

摘要翻译： 本发明涉及酰化氨基酸酰氨基吡唑和式I的相关化合物。本发明还涉及包含这种化合物或其药学上可接受的盐形式的药物组合物。 本发明进一步涉及一种抑制β-淀粉样蛋白肽释放和/或合成的方法，一种抑制γ-分泌酶活性的方法，以及用于治疗与β-淀粉样蛋白肽生产相关的神经障碍的方法。 该方法包括向宿主施用包含有效量的式I化合物的药物制剂。式I化合物可用于预防和治疗阿尔茨海默氏病。

公开(公告)号：US07674900B2

公开(公告)日：2010-03-09

申请号：US11578708

申请日：2005-05-11

申请人： Albert Sun-Chi Chan ,  Gang Chen ,  Rongwei Guo ,  Jing Wu

发明人： Albert Sun-Chi Chan ,  Gang Chen ,  Rongwei Guo ,  Jing Wu

IPC分类号： C07F9/6544

CPC分类号： B01J31/2409 ,  B01J2231/44 ,  B01J2531/16 ,  B01J2531/821 ,  B01J2531/822 ,  B01J2531/824 ,  B01J2531/827 ,  B01J2531/828 ,  B01J2531/847 ,  C07F9/6512 ,  C07F15/0066

摘要： The present invention provides bipyrimidinyl diphosphine compounds of the formula wherein R is optionally substituted alkyl, cycloalkyl, aryl or heteroaryl; R′ and R″ are independently optionally substituted alkyl, cycloalkyl, aryl or heteroaryl; or an enantiomer thereof; or an enantiomeric mixture thereof. The compounds of the formula (I) are chiral atropisomeric bipyrimidinyl diphosphine compounds and, thus, may be employed as ligands to generate chiral transition metal catalysts which may be applied in a variety of asymmetric reactions, e.g., in palladium catalyzed asymmetric allylic substitution reactions. The compounds of the present invention are easily accessible in high enantiomeric purity according to the methods disclosed herein.

摘要翻译： 本发明提供下式的二嘧啶基二膦化合物其中R是任选取代的烷基，环烷基，芳基或杂芳基; R'和R“独立地是任选取代的烷基，环烷基，芳基或杂芳基; 或其对映体; 或其对映体混合物。 式（I）的化合物是手性的不对称双嘧啶基二膦化合物，因此可以用作配体以产生手性过渡金属催化剂，其可以用于各种不对称反应，例如钯催化的不对称烯丙基取代反应。 根据本文公开的方法，本发明的化合物可以以高对映体纯度容易地获得。

公开(公告)号：US07660805B2

公开(公告)日：2010-02-09

申请号：US11010303

申请日：2004-12-14

申请人： Sammy Chan ,  Nicole Ai Ling Lam ,  Alison Joan Lennon ,  Jing Wu

发明人： Sammy Chan ,  Nicole Ai Ling Lam ,  Alison Joan Lennon ,  Jing Wu

IPC分类号： G06F7/00 ,  G06F17/00

CPC分类号： G06F17/30292 ,  Y10S707/99942

摘要： A query system (1600) is formed by a wizard (1605) operating to form a data server (1610) arranged to serve data from a data source (1615) to a client application (1630), each of the wizard, the client application, the data server and the data source being linked by a communications network (1650). The data source has native data types and data arranged in a data structure. The wizard operates to firstly identify the data source over the network. The wizard further provides an application program for traversing and examining at least one of the data structure and data of the identified data source. The application program is operable to generate a representative schema (1614) comprising mapping data for converting the native data types to predetermined data types of a common data model. The wizard further operates to create a server application (1612) adapted to use the representative schema to convert the data from the native data types to the predetermined data types and to serve the data to the client application according to the common data model over the network. The representative schema and the server application thereby form the data server generated by the wizard. Once formed, the data server may receive and respond to queries from the client application, which may have no knowledge of the native data types, but which are directed to the data source.

摘要翻译： 由向导（1605）形成查询系统（1605），该向导（1605）操作以形成数据服务器（1610），该数据服务器被布置为从数据源（1615）向客户端应用（1630）提供数据，每个向导，客户端应用 ，数据服务器和数据源由通信网络（1650）链接。 数据源具有以数据结构排列的原生数据类型和数据。 该向导将首先通过网络识别数据源。 向导还提供了用于遍历和检查所识别的数据源的数据结构和数据中的至少一个的应用程序。 应用程序可操作以产生代表性模式（1614），其包括用于将本地数据类型转换为公共数据模型的预定数据类型的映射数据。 向导进一步操作以创建适于使用代表性模式将数据从本机数据类型转换为预定数据类型的服务器应用程序（1612），并根据网络上的公共数据模型将数据提供给客户端应用程序 。 因此，代表性模式和服务器应用程序形成了向导生成的数据服务器。 一旦形成，数据服务器可以接收并响应来自客户端应用程序的查询，客户端应用程序可能不知道本机数据类型，但是哪些是针对数据源的。