公开(公告)号：US11773383B2

公开(公告)日：2023-10-03

申请号：US17349933

申请日：2021-06-17

申请人： Jing Wu ,  Lingqia Su ,  Yan Huang

发明人： Jing Wu ,  Lingqia Su ,  Yan Huang

IPC分类号： C12N9/92 ,  C12N9/18 ,  C12N9/10 ,  C12N15/75

CPC分类号： C12N9/18 ,  C12N9/1051 ,  C12N9/92 ,  C12N15/75 ,  C12Y204/01245 ,  C12Y301/01074 ,  C12Y503/01005

摘要： Disclosed is a method for promoting extracellular expression of proteins in B. subtilis using cutinase, which belongs to the technical fields of genetic engineering, enzyme engineering and microbial engineering. It teaches co-expressing a cutinase mutant and a target protein in B. subtilis to promote extracellular expression of the target protein which is naturally located inside cells. The target protein includes xylose isomerase, 4,6-α-glucosyltransferase, 4-α-glucosyltransferase, trehalose synthase, branching enzyme and the like. The invention can achieve extracellular expression of intracellularly localized target protein, improve the production efficiency, reduce the production cost and simplify the subsequent extraction process.

公开(公告)号：US10988550B2

公开(公告)日：2021-04-27

申请号：US16196768

申请日：2018-11-20

申请人： Jing Wu ,  Sheng Chen ,  Jun Liu

发明人： Jing Wu ,  Sheng Chen ,  Jun Liu

IPC分类号： C08B30/18 ,  A23L29/269 ,  C12P19/04 ,  C08L3/02 ,  C08B31/00

摘要： Disclosed is a method for preparing a resistant dextrin product by using compound enzyme treatment. Starch is first subjected to high-temperature acidolysis to obtain pyrodextrin which is used as the substrate for the enzyme treatment. A compound enzyme reaction system including a starch branching enzyme and a CGTase is used for preparing the resistant dextrin product. The starch branching enzyme and the CGTase are added simultaneously or sequentially to treat the pyrodextrin to further increase the content of the resistant component in the product. The content of the resistant component of the enzyme treated product reaches up to 65.3%, a 21.3% increase from that of the pyrodextrin before the enzyme treatment.

公开(公告)号：US20190032062A1

公开(公告)日：2019-01-31

申请号：US15891564

申请日：2018-02-08

申请人： Jing Wu ,  Quan Zhang ,  Liming Liu ,  Xiulai Chen ,  Jia Liu ,  Qiuling Luo

发明人： Jing Wu ,  Quan Zhang ,  Liming Liu ,  Xiulai Chen ,  Jia Liu ,  Qiuling Luo

IPC分类号： C12N15/70 ,  C12P19/26 ,  C12N9/90 ,  C12N9/10 ,  C12P19/24

CPC分类号： C12N15/70 ,  C12N9/1096 ,  C12N9/90 ,  C12P19/24 ,  C12P19/26 ,  C12Y504/0201

摘要： The present invention relates to the field of biotechnology engineering. It provides a recombinant Escherichia coli that can produce fructosylated chondroitin with high yields and the method for constructing the recombinant strain. The present invention discloses a method for constructing an expression plasmid pETM6R1-RBS-glmM-GGGS-glmS that contains a glmM and a glmS gene under regulation of ribosome binding sites. The recombinant plasmid was transformed into E. coli K4 to obtain a recombinant E. coli strain ZQ33 that can produce fructosylated chondroitin up to 3.99 g·L−1 by fed-batch fermentation in a 5-L fermentor, which was increased by 108.90% compared to that of the wild type strain.

公开(公告)号：US20170117614A1

公开(公告)日：2017-04-27

申请号：US15283530

申请日：2016-10-03

申请人： Jing Wu ,  Jianchun Mai ,  Chao Wang

发明人： Jing Wu ,  Jianchun Mai ,  Chao Wang

IPC分类号： H01Q1/24 ,  H01Q1/38 ,  H01Q1/48 ,  H01Q1/22 ,  H01Q1/36

CPC分类号： H01Q1/243 ,  H01Q1/2291 ,  H01Q5/328 ,  H01Q9/42 ,  H01Q21/28 ,  H01Q21/30

摘要： An antenna system with a full metal back cover is provided in the present disclosure. The antenna system includes a metal back cover with a main body, a first sidewall and a second sidewall opposite to each other, a circuit board with a main ground point, a first antenna module and a second antenna module electrically connected to the circuit board. A first gap is formed between the first sidewall and the main body, and a second gap is formed between the second sidewall and the main body; the metal back cover is divided into a first metal portion including the first sidewall and a second metal portion including the second sidewall. The first metal portion includes a top slit penetrating through an edge of the first sidewall, and the second metal portion includes a bottom slit penetrating through an edge of the second sidewall.

公开(公告)号：US09064102B2

公开(公告)日：2015-06-23

申请号：US11853555

申请日：2007-09-11

申请人： Jing Wu ,  James Ronald Flesch

发明人： Jing Wu ,  James Ronald Flesch

IPC分类号： G06F21/34 ,  H04L9/32 ,  H04W12/04 ,  H04L29/06

CPC分类号： G06F21/34 ,  H04L9/3226 ,  H04L65/4084 ,  H04L2209/60 ,  H04L2209/80 ,  H04W12/04

摘要： A method, system and device for providing secure access to multimedia content received by a networked digital storage device, such as a set-top box. A mobile device, such as a mobile telephone, obtains appropriate security binding information and application software when coupled to the networked digital storage device at its end user location. The mobile device uploads the security binding information to a randomly located temporary hosting device at its place-shifted location when coupled thereto and, through a logical binding with the temporary hosting device, enables a secure, remote session. The secure binding and transfer of appropriate keys allow the remote hosting device to securely access premium or protected digital material/services available at the networked digital storage device. After completion of the secure, remote access session, termination occurs and the security binding information and the computing activity residue can be removed from the mobile device and the temporary hosting device.

摘要翻译： 一种用于提供对联网的数字存储设备（诸如机顶盒）所接收的多媒体内容的安全访问的方法，系统和设备。 诸如移动电话的移动设备在其最终用户位置处耦合到联网数字存储设备时获得适当的安全绑定信息和应用软件。 移动设备在耦合到其位置移动位置时将安全绑定信息上传到随机定位的临时托管设备，并且通过与临时托管设备的逻辑绑定来启用安全的远程会话。 适当密钥的安全绑定和传输允许远程托管设备安全地访问网络数字存储设备上可用的高级或受保护的数字材料/服务。 在完成安全的远程访问会话之后，终止并且可以从移动设备和临时托管设备移除安全绑定信息和计算活动残留。

公开(公告)号：US20130209490A1

公开(公告)日：2013-08-15

申请号：US13852079

申请日：2013-03-28

申请人： Alan Buckler ,  Chao-Min Chen ,  Chantale T. Guy ,  Jeffrey Hewett ,  Chris Lu ,  Jing Wu

发明人： Alan Buckler ,  Chao-Min Chen ,  Chantale T. Guy ,  Jeffrey Hewett ,  Chris Lu ,  Jing Wu

IPC分类号： A61K38/18 ,  A61K38/16 ,  A61K31/7088 ,  A61K45/06 ,  A61K39/395 ,  G01N33/50

CPC分类号： A61K38/1875 ,  A61K31/7088 ,  A61K38/16 ,  A61K39/395 ,  A61K45/06 ,  G01N33/5091 ,  G01N33/74 ,  G01N2333/495 ,  G01N2800/7052 ,  A61K2300/00

摘要： The present invention provides novel methods of inhibiting fibrosis, as well as methods of treating or inhibiting fibrotic disorders, using BMP9 and/or BMP10 antagonists. The present invention also provides methods of assessing whether a subject has or is at risk of developing a fibrotic disorder by detecting levels of BMP9 and/or BMP10. Further provided are methods of assessing the efficacy of a treatment regimen for treating a fibrotic disorder by detecting and comparing pre-treatment levels of BMP9 and BMP10 with post-treatment levels of BMP9 and BMP10.

摘要翻译： 本发明提供使用BMP9和/或BMP10拮抗剂抑制纤维化的新方法，以及治疗或抑制纤维化病症的方法。 本发明还提供了通过检测BMP9和/或BMP10的水平来评估受试者是否具有发展为纤维化疾病的风险的方法。 还提供了通过检测和比较BMP9和BMP10的预处理水平与BMP9和BMP10的后处理水平来评估治疗方案用于治疗纤维化病症的功效的方法。

公开(公告)号：US20120147157A1

公开(公告)日：2012-06-14

申请号：US12964452

申请日：2010-12-09

申请人： Jing Wu ,  Ajay K. Luthra ,  Paul A. Clancy ,  James R. Flesch

发明人： Jing Wu ,  Ajay K. Luthra ,  Paul A. Clancy ,  James R. Flesch

IPC分类号： H04N13/04

CPC分类号： H04N13/341 ,  H04N13/398

摘要： In a method of managing 3D video stream, 3D video stream is output to a connected display. The connected display displays the 3D video stream after a delay associated with the connected display. A timing offset is determined based on the delay. The timing offset is operable to be used to synchronize shutter operations of at least one shutter glasses with the presentation of the 3D video stream. A shutter control signal based on a frame start indication from the 3D video stream is transmitted. The frame start indication is operable to be used to determine a frame rate of the 3D video stream, and the shutter control signal is operable to be used to maintain synchronization of the shutter operations of the at least one shutter glasses with the presentation of the 3D video stream at the connected display.

摘要翻译： 在管理3D视频流的方法中，将3D视频流输出到连接的显示器。 所连接的显示器在与连接的显示器相关联的延迟之后显示3D视频流。 基于延迟确定定时偏移。 定时偏移可操作地用于将至少一个快门眼镜的快门操作与3D视频流的呈现同步。 发送基于来自3D视频流的帧开始指示的快门控制信号。 帧开始指示可操作以用于确定3D视频流的帧速率，并且快门控制信号可操作以用于保持至少一个快门眼镜的快门操作与3D的呈现的同步 连接显示器上的视频流。

公开(公告)号：US20110034691A1

公开(公告)日：2011-02-10

申请号：US12731053

申请日：2010-03-24

申请人： Michael S. Dappen ,  Jacek J. Jagodzinski ,  Lee H. Latimer ,  Jing Wu ,  Jollie Duaine Godfrey, JR. ,  Robert Michael Matunas ,  Thomas W. Drabb ,  Gary Probst

发明人： Michael S. Dappen ,  Jacek J. Jagodzinski ,  Lee H. Latimer ,  Jing Wu ,  Jollie Duaine Godfrey, JR. ,  Robert Michael Matunas ,  Thomas W. Drabb ,  Gary Probst

IPC分类号： C07D471/04 ,  C07D231/12 ,  C07D401/12 ,  C07D231/16

CPC分类号： C07D231/12 ,  C07D471/04

摘要： The present invention provides methods, i.e., scalable or large-scale processes for the production of fused, tricyclic sulfonamido analogs, such as substituted or unsubstituted 5-(aryl-sulfonyl)-4,5-dihydro-1H-pyrazolo[4,3-c]quinolines and 5-(heteroaryl-sulfonyl)-4,5-dihydro-1H-pyrazolo[4,3-c]quinolines. Exemplary methods of the invention include an intra-molecular cyclization step, in which a carbon-nitrogen bond is formed, and which employs a copper-based catalyst that contains at least one organic ligand, such as DMEDA. The invention further provides compounds, which are useful as intermediates in the methods of the invention.

摘要翻译： 本发明提供了用于生产融合的三环磺酰氨基类似物的可扩展或大规模方法，例如取代或未取代的5-（芳基 - 磺酰基）-4,5-二氢-1H-吡唑并[4,3 -c]喹啉和5-（杂芳基 - 磺酰基）-4,5-二氢-1H-吡唑并[4,3-c]喹啉。 本发明的示例性方法包括其中形成碳 - 氮键的分子内环化步骤，并且其使用含有至少一种有机配体如DMEDA的铜基催化剂。 本发明还提供了可用作本发明方法中的中间体的化合物。

公开(公告)号：US07754712B2

公开(公告)日：2010-07-13

申请号：US11951097

申请日：2007-12-05

申请人： Simeon Bowers ,  Albert W. Garofalo ,  Roy K. Hom ,  Andrei W. Konradi ,  Matthew N. Mattson ,  Martin L. Neitzel ,  Christopher M. Semko ,  Anh P. Truong ,  Jing Wu ,  Ying-zi Xu

发明人： Simeon Bowers ,  Albert W. Garofalo ,  Roy K. Hom ,  Andrei W. Konradi ,  Matthew N. Mattson ,  Martin L. Neitzel ,  Christopher M. Semko ,  Anh P. Truong ,  Jing Wu ,  Ying-zi Xu

IPC分类号： A61K31/5365 ,  A61K31/437 ,  A61K31/4985 ,  A61P25/28 ,  C07D497/04 ,  C07D497/14 ,  C07D471/14

CPC分类号： C07D451/14 ,  C07D471/08 ,  C07D471/18 ,  C07D471/22 ,  C07D498/08

摘要： The invention provides N-cyclic sulfonamido compounds and salts of Formula I: wherein A is as described in the specification and R1 and R2 combine to form a [3.3.1] or a [3.2.1] ring system, where the nitrogen is attached to the two bridgehead carbons, and the [3.3.1] or [3.2.1] ring systems are optionally fused with an heteroaryl or heterocycloalkyl ring. Compounds of Formula I are useful in treating or preventing cognitive disorders, such as Alzheimer's Disease. The invention also encompasses pharmaceutical compositions comprising compounds of Formula I, methods of treating cognitive disorders, such as Alzheimer's disease, and the intermediates useful in preparing the compounds of Formula I.

摘要翻译： 本发明提供了N-环磺酰氨基化合物和式I的盐：其中A如说明书中所述，并且R 1和R 2结合形成[3.3.1]或[3.2.1]环体系，其中氮被连接 并且[3.3.1]或[3.2.1]环体系任选地与杂芳基或杂环烷基环稠合。 式I的化合物可用于治疗或预防认知障碍，例如阿尔茨海默病。 本发明还包括含有式I化合物的药物组合物，治疗认知障碍的方法，例如阿尔茨海默氏病，以及可用于制备式I化合物的中间体。

公开(公告)号：US07432379B2

公开(公告)日：2008-10-07

申请号：US10837231

申请日：2004-04-30

申请人： Jay S. Tung ,  Albert W. Garofalo ,  Michael A. Pleiss ,  Jing Wu ,  David W. G. Wone ,  Ashley C. Guinn ,  Darren B. Dressen ,  R. Jeffrey Neitz ,  Jennifer Marugg ,  Martin Neitzel

发明人： Jay S. Tung ,  Albert W. Garofalo ,  Michael A. Pleiss ,  Jing Wu ,  David W. G. Wone ,  Ashley C. Guinn ,  Darren B. Dressen ,  R. Jeffrey Neitz ,  Jennifer Marugg ,  Martin Neitzel

IPC分类号： C07D401/12

CPC分类号： C07D401/14 ,  C07D231/16 ,  C07D231/40 ,  C07D401/12 ,  C07D403/12 ,  C07D409/12 ,  C07D413/12 ,  C07D417/12 ,  C07D453/02 ,  C07D471/04 ,  C07D471/08 ,  C07D487/08

摘要： Disclosed are compounds that are bradykinin B1 receptor antagonists and are useful for treating diseases, or relieving adverse symptoms associated with disease conditions, in mammals mediated by bradykinin B1 receptor. Certain of the compounds exhibit increased potency and are also expected to exhibit increased duration of action.

摘要翻译： 公开了缓激肽B 1受体拮抗剂的化合物，并且可用于治疗由缓激肽B 1受体介导的哺乳动物中疾病或与疾病状况相关的不良症状。 某些化合物表现出增加的效力，并且还预期表现出增加的作用持续时间。