公开(公告)号：US20240294991A1

公开(公告)日：2024-09-05

申请号：US18652741

申请日：2024-05-01

Applicant: Becton, Dickinson and Company

Inventor： Christina Fan ,  Stephen P.A. Fodor ,  Glenn Fu ,  Geoffrey Richard Facer ,  Julie Wilhelmy

IPC: C12Q1/6888 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q1/6881

CPC classification number: C12Q1/6888 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q1/6881 ,  C12Q2600/158 ,  C12Q2600/16

Abstract: The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).

公开(公告)号：US11702706B2

公开(公告)日：2023-07-18

申请号：US17192814

申请日：2021-03-04

Applicant: Becton, Dickinson and Company

Inventor： Christina Fan ,  Stephen P. A. Fodor ,  Glenn Fu ,  Geoffrey Richard Facer ,  Julie Wilhelmy

IPC: C12Q1/68 ,  C12Q1/6888 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q1/6881

CPC classification number: C12Q1/6888 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q1/6881 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q1/6874 ,  C12Q2563/159 ,  C12Q2563/185

Abstract: The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).

公开(公告)号：US11608497B2

公开(公告)日：2023-03-21

申请号：US15806174

申请日：2017-11-07

Applicant: Becton, Dickinson and Company

Inventor： Jing Hu ,  Christina Fan ,  David Rosenfeld ,  Jue Fan ,  Elisabeth Marie Walczak

IPC: C12N15/10 ,  G16B45/00 ,  C12Q1/6869 ,  G16B20/00

Abstract: Disclosed herein are methods and systems for classifying cell labels, for example identifying a signal cell label. In some embodiments, the method comprises: obtaining sequencing data of barcoded targets created using targets in cells barcoded using barcodes, wherein a barcode comprises a cell label and a molecular label. After ranking the cell labels, a minimum of a second derivative plot of a cumulative sum plot can be determined. Using the methods, a cell label can be classified as a signal cell label or a noise cell label based on the number of molecular labels with distinct sequences associated with the cell label and a cell label threshold.

公开(公告)号：US20220348904A1

公开(公告)日：2022-11-03

申请号：US17745806

申请日：2022-05-16

Applicant: Becton, Dickinson and Company

Inventor： Eleen Shum ,  Christina Fan

IPC: C12N15/10 ,  C12Q1/6816

Abstract: Disclosed herein include systems, methods, compositions, and kits for molecular barcoding on the 5′-end of a nucleic acid target. After barcoding a nucleic acid target using an oligonucleotide barcode comprising a target binding region and a molecular label to generate a barcoded nucleic acid molecule, an oligonucleotide comprising a complement of the target binding region can be added to generate a barcoded nucleic acid molecule comprising the target-binding region and the complement of the target-binding region. A stem loop is formed with intra-molecular hybridization of the barcoded nucleic acid molecule, which can be extended to generate an extended barcoded nucleic acid molecule comprising the molecular label and a complement of the molecular label.

公开(公告)号：US20220333185A1

公开(公告)日：2022-10-20

申请号：US17684289

申请日：2022-03-01

Applicant: Becton, Dickinson and Company

Inventor： Glenn Fu ,  Craig Betts ,  Christina Fan ,  Gretchen Yinbon Lam

IPC: C12Q1/6855 ,  C12Q1/6806

Abstract: The disclosure provides for methods, compositions, systems, devices, and kits for whole transcriptome amplification using stochastic barcodes.

公开(公告)号：US11460468B2

公开(公告)日：2022-10-04

申请号：US15715028

申请日：2017-09-25

Applicant: Cellular Research, Inc. ,  Becton, Dickinson and Company

Inventor： Christina Fan ,  Christina Chang ,  Eleen Shum ,  Olaf Zoellner ,  Nidhanjali Bansal ,  James Ghadiali ,  Gretchen Yinbon Lam ,  Eric Jensen ,  Jurg Rohrer ,  Brent Gaylord ,  Jody Martin ,  Jason G. Vidal

IPC: G01N33/566 ,  G16B99/00 ,  G01N33/50 ,  G01N33/543 ,  G01N33/68 ,  C12Q1/6804 ,  G16Z99/00 ,  C12N15/10 ,  C12Q1/6806 ,  C12Q1/6823 ,  C12Q1/6869 ,  C12Q1/68 ,  G16B25/10 ,  G16H20/13 ,  G16H10/20

Abstract: Some embodiments disclosed herein provide a plurality of compositions each comprising a protein binding reagent conjugated with an oligonucleotide. The oligonucleotide comprises a unique identifier for the protein binding reagent it is conjugated with, and the protein binding reagent is capable of specifically binding to a protein target. Further disclosed are methods and kits for quantitative analysis of a plurality of protein targets in a sample and for simultaneous quantitative analysis of protein and nucleic acid targets in a sample. Also disclosed herein are systems and methods for preparing a labeled biomolecule reagent, including a labeled biomolecule agent comprising a protein binding reagent conjugated with an oligonucleotide.

公开(公告)号：USRE48913E1

公开(公告)日：2022-02-01

申请号：US16535080

申请日：2019-08-08

Applicant: Becton, Dickinson and Company

Inventor： Stephen P. A. Fodor ,  Christina Fan ,  Glenn Fu ,  Geoffrey Facer

IPC: G06K19/06 ,  C12Q1/6813 ,  C12Q1/6816

Abstract: The disclosure provides for methods, compositions, systems, devices, and kits for determining the number of distinct targets in distinct spatial locations within a sample. In some examples, the methods include: stochastically barcoding the plurality of targets in the sample using a plurality of stochastic barcodes, wherein each of the plurality of stochastic barcodes comprises a spatial label and a molecular label; estimating the number of each of the plurality of targets using the molecular label; and identifying the spatial location of each of the plurality of targets using the spatial label. The method can be multiplexed.

公开(公告)号：US20200255888A1

公开(公告)日：2020-08-13

申请号：US16788221

申请日：2020-02-11

Applicant: Becton, Dickinson and Company

Inventor： Devon Jensen ,  David Rosenfeld ,  Christina Fan ,  Jue Fan

IPC: C12Q1/6809 ,  C12Q1/686 ,  G16B30/00 ,  G16B50/30

Abstract: Disclosed herein include systems, methods, compositions, and kits for determining numbers of occurrences of variants (e.g., transcript variants) of targets (e.g., gene targets) in cells and/or samples. In some embodiments, modification target sites (e.g., polyadenylation sites) and usage thereof are determined. Whole transcriptome amplification analysis can be performed and the sequencing reads obtained can be analyzed to identify polyadenylation sites (and usage thereof) for the design of customized primer panels for targeted scRNAseq experiments.

公开(公告)号：US20160289669A1

公开(公告)日：2016-10-06

申请号：US15004618

申请日：2016-01-22

Applicant: Becton, Dickinson and Company

Inventor： Christina Fan ,  Stephen P.A. Fodor ,  Glenn Fu ,  Geoff Facer ,  David Stern ,  Janice Lai ,  Ari Chaney ,  Phillip Spuhler ,  Sixing Li ,  Xiaohua Chen Huang ,  Kimberly R. Metzler ,  Elisabeth Marie Walczak

IPC: C12N15/10 ,  C12Q1/68 ,  B01L3/00

CPC classification number: C12N15/1065 ,  B01L3/502715 ,  B01L3/502738 ,  B01L3/502746 ,  B01L3/502761 ,  B01L2200/026 ,  B01L2200/0647 ,  B01L2300/06 ,  B01L2300/0654 ,  B01L2300/0819 ,  B01L2300/0877 ,  B01L2300/12 ,  B01L2300/18 ,  B01L2400/082 ,  C12Q1/6846 ,  C12Q1/6874 ,  G01N35/0098 ,  C12Q2521/107 ,  C12Q2525/173 ,  C12Q2525/185 ,  C12Q2531/113 ,  C12Q2535/122 ,  C12Q2563/143 ,  C12Q2563/149

Abstract: Disclosed herein are devices and systems comprising a) a substrate comprising at least 100 microwells and a plurality of beads, wherein a plurality of the at least 100 microwells each contain a single bead, and wherein the ratio of the average diameter of the microwells to the diameter of the beads ranges from about 1.2 to about 1.8; b) a flow cell in fluid communication with the substrate; and c) at least one inlet port and at least one outlet port, wherein the at least one inlet port and at least one outlet port are capable of directing a flow of a fluid through the flow cell, thereby contacting the microwells with the fluid.

Abstract translation: 本文公开的装置和系统包括a）包含至少100个微孔和多个珠的基底，其中多个至少100个微孔各自包含单个珠，并且其中微孔的平均直径与 珠的直径范围为约1.2至约1.8; b）与衬底流体连通的流动池; 以及c）至少一个入口端口和至少一个出口端口，其中所述至少一个入口端口和至少一个出口端口能够引导流体流过所述流动池，从而使所述微孔与所述流体接触。