公开(公告)号：US20240011021A1

公开(公告)日：2024-01-11

申请号：US18173735

申请日：2023-02-23

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Rustem F. ISMAGILOV ,  Matthew S. CURTIS ,  Mary ARRASTIA ,  David A. SELCK ,  Mitchell GUTTMAN

IPC: C12N15/10 ,  B01L3/00 ,  C12Q1/6855 ,  G01N33/58 ,  G01N33/68 ,  C12Q1/6806

CPC classification number: C12N15/1065 ,  B01L3/502715 ,  C12Q1/6855 ,  G01N33/58 ,  G01N33/68 ,  C12Q1/6806 ,  B01L3/502738 ,  B01L2200/16 ,  B01L2300/161 ,  B01L2300/0861 ,  B01L2200/027 ,  B01L2200/141 ,  B01L2300/0816 ,  B01L2400/065 ,  C40B30/04

Abstract: Methods, systems and related compositions are provided to perform single-cell marking of a nucleic acid and/or protein in a sample based on in-cell or in-organelle barcoding of nucleic acid and/or protein complexes of the cell or organelle; the methods and systems herein described are configured to provide in-cell or in-organelle single-cell marked nucleic acid and/or protein complexes comprising a single-cell, cell-specific, or a single-cell organelle-specific marker.

公开(公告)号：US20210079447A1

公开(公告)日：2021-03-18

申请号：US16927496

申请日：2020-07-13

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Rustem F. ISMAGILOV ,  Jacob T. BARLOW ,  Said R. BOGATYREV

IPC: C12Q1/686 ,  C12Q1/6888

Abstract: Provided herein are methods and systems for absolute quantification of a target 16S rRNA and/or of a target prokaryotic taxon, based on amplifying and sequencing a same 16S rRNA recognition segment in which target 16S rRNA conserved regions flank 16S rRNA variable regions, conserved and variable among a plurality of sample 16S rRNAs and/or of a sample prokaryotic taxon of higher taxonomic rank with respect to the target taxon. In the methods and systems, absolute abundance of the a plurality of sample 16S rRNAs and/or of the sample prokaryotic taxon detected by the amplifying, is multiplied by the relative abundance of the target 16S rRNA and/or of a target prokaryotic taxon detected by the sequencing to provide the absolute quantification in accordance with method and systems of the disclosure.

公开(公告)号：US20190144854A1

公开(公告)日：2019-05-16

申请号：US16141901

申请日：2018-09-25

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Rustem F. ISMAGILOV ,  Matthew S. CURTIS ,  Mary ARRASTIA ,  David A. SELCK ,  Mitchell GUTTMAN

IPC: C12N15/10

Abstract: Methods, systems and related compositions are provided to perform single-cell marking of a nucleic acid and/or protein in a sample based on in-cell or in-organelle barcoding of nucleic acid and/or protein complexes of the cell or organelle; the methods and systems herein described are configured to provide in-cell or in-organelle single-cell marked nucleic acid and/or protein complexes comprising a single-cell, cell-specific, or a single-cell organelle-specific marker.

公开(公告)号：US20190118177A1

公开(公告)日：2019-04-25

申请号：US16141707

申请日：2018-09-25

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Rustem F. ISMAGILOV ,  Eugenia KHOROSHEVA ,  Dmitriy V. ZHUKOV

IPC: B01L3/00 ,  C12Q1/6855

Abstract: A device for allowing compartmentalized reactions with minimized cross-contamination between the compartments, utilizing a delivery of material by loading wells to pooling wells, such that the pooling wells can be additively provided with reactants while maintaining isolation between the pooling wells. The use of geometric properties is used to facilitate transmission of fluids/droplets without the need for hydrophilic surfaces.

公开(公告)号：US20190111423A1

公开(公告)日：2019-04-18

申请号：US15539551

申请日：2015-12-23

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Rustem F. ISMAGILOV ,  Stefano BEGOLO ,  Jesus Rodriguez MANZANO

IPC: B01L3/00 ,  C12Q1/686 ,  B01L7/00

Abstract: Disclosed herein are devices and methods for storing, processing, preparing and/or analyzing samples. The inventions herein also relate to strategies and methods for automating device operations and for combining multiple devices in an integrated platform.

公开(公告)号：US20180321137A1

公开(公告)日：2018-11-08

申请号：US15774004

申请日：2016-11-04

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Rustem F. ISMAGILOV ,  Jesus Rodriguez MANZANO ,  Mikhail KARYMOV ,  David A. SELCK ,  Stefano BEGOLO ,  Erik B. JUE ,  Dmitriy V. ZHUKOV

IPC: G01N21/25 ,  G06K9/00 ,  G06T7/90 ,  G01J3/50 ,  G01J3/02 ,  C12Q1/6844

CPC classification number: G01N21/253 ,  C12Q1/6844 ,  G01J3/0272 ,  G01J3/46 ,  G01J3/50 ,  G01N2201/0221 ,  G06K9/00134 ,  G06T7/00 ,  G06T7/90 ,  G06T2207/10024 ,  G06T2207/30024 ,  C12Q2527/101 ,  C12Q2537/165 ,  C12Q2563/159

Abstract: The present invention relates to methods, computer readable medium and systems for detecting and counting single nucleic acid molecules confined in nanoliter volumes using an unmodified camera, such as a cell phone camera. In particular, it identifies colorimetric amplification-indicator dyes that are compatible with the spectral sensitivity of standard mobile phones. The invention further provides an optimal ratiometric image-process for a selected dye to achieve a readout that is robust to lighting conditions and camera hardware and provides unambiguous quantitative results, even for colorblind users.

公开(公告)号：US20160114322A1

公开(公告)日：2016-04-28

申请号：US14785480

申请日：2014-04-18

Applicant: Qichao PAN ,  Mikhail KARYMOV ,  Toan HUYNH ,  George SAWICKI ,  Stefano BEGOLO ,  Wenbin DU ,  CALIFORNIA INSTITUTE OF TECHNOLOGY ,  UNIVERSITY OF CHICAGO

Inventor： Rustem F. ISMAGILOV ,  Liang MA ,  Qichao PAN ,  Mikhail KARYMOV ,  Toan HUYNH ,  George SAWICKI ,  Stefano BEGOLO ,  Wenbin DU

IPC: B01L3/00 ,  C12Q1/68 ,  C12Q1/02

CPC classification number: B01L3/502715 ,  B01F13/0071 ,  B01F13/0083 ,  B01L3/502738 ,  B01L9/527 ,  B01L2200/025 ,  B01L2200/026 ,  B01L2200/0605 ,  B01L2200/0642 ,  B01L2300/045 ,  B01L2300/047 ,  B01L2300/048 ,  B01L2300/0627 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/0864 ,  B01L2400/0487 ,  B01L2400/065 ,  C12M23/44 ,  C12M23/46 ,  C12M25/06 ,  C12Q1/02 ,  C12Q1/025 ,  C12Q1/686 ,  G01N2035/00158

Abstract: Provided herein are methods, compositions, and devices for the parallel handling of samples, such as cells or other biological samples. The methods, compositions, and devices are suited for multiple levels of analysis, including genetic and functional assays, of samples.

Abstract translation: 本文提供了用于平行处理样品（例如细胞或其他生物样品）的方法，组合物和装置。 方法，组合物和装置适合于多种水平的分析，包括样品的遗传和功能测定。