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    Methods for amplifying nucleic acid using tag-mediated displacement
    21.
    发明授权
    Methods for amplifying nucleic acid using tag-mediated displacement 有权

    公开(公告)号：US10214760B2

    公开(公告)日：2019-02-26

    申请号：US14928796

    申请日：2015-10-30

    Applicant: Gen-Probe Incorporated

    Inventor： Norman C. Nelson ,  Margarita Batranina-Kaminsky

    IPC: C12Q1/68 ,  C12P19/34 ,  C12Q1/6855 ,  C07H21/04 ,  C12Q1/6844

    Abstract: Disclosed are methods for amplifying a nucleic acid target region using an amplification oligomer comprising a target-binding segment and a heterologous displacer tag situated 5′ to the target-binding segment. Initiation of an amplification reaction from the tagged amplification oligomer produces an amplicon comprising the displacer tag, such that once the complement of the displacer tag has been incorporated into a second amplicon, a displacer oligonucleotide having a sequence substantially corresponding to the displacer tag sequence is used to participate in subsequent rounds of amplification for displacement of an extension product primed from a site within the second amplicon 5′ to the displacer priming site. Also disclosed are related kits and reaction mixtures comprising the displacer-tagged amplification oligomer and corresponding displacer oligonucleotide.

    Methods for amplifying nucleic acid using tag-mediated displacement
    23.
    发明授权
    Methods for amplifying nucleic acid using tag-mediated displacement 有权
    使用标签介导位移扩增核酸的方法

    公开(公告)号：US09175337B2

    公开(公告)日：2015-11-03

    申请号：US14344372

    申请日：2012-09-21

    Applicant: Gen-Probe Incorporated

    Inventor： Norman C. Nelson ,  Margarita Batranina-Kaminsky

    IPC: C12Q1/68 ,  C07H21/02

    CPC classification number: C12P19/34 ,  C12Q1/6844 ,  C12Q1/6855 ,  C12Q2537/137

    Abstract: Disclosed are methods for amplifying a nucleic acid target region using an amplification oligomer comprising a target-binding segment and a heterologous displacer tag situated 5′ to the target-binding segment. Initiation of an amplification reaction from the tagged amplification oligomer produces an amplicon comprising the displacer tag, such that once the complement of the displacer tag has been incorporated into a second amplicon, a displacer oligonucleotide having a sequence substantially corresponding to the displacer tag sequence is used to participate in subsequent rounds of amplification for displacement of an extension product primed from a site within the second amplicon 5′ to the displacer priming site. Also disclosed are related kits and reaction mixtures comprising the displacer-tagged amplification oligomer and corresponding displacer oligonucleotide.

    Abstract translation: 公开了使用包含靶结合片段和位于靶结合片段5'的异源置换标签的扩增寡聚物来扩增核酸靶区的方法。 从标记的扩增寡聚物引发扩增反应产生包含置换器标签的扩增子，使得一旦置换剂标签的补体已经被并入第二扩增子中，则使用具有基本上对应于置换标签序列的序列的置换寡核苷酸 参与随后的放大扩增，以将从第二扩增子5'内的位点引发的延伸产物置换到置换剂引发位点。 还公开了包含置换剂标记的扩增寡聚物和相应置换剂寡核苷酸的相关试剂盒和反应混合物。

    Methods and compositions for nucleic acid amplification
    26.
    发明授权
    Methods and compositions for nucleic acid amplification 有权

    公开(公告)号：US10407723B2

    公开(公告)日：2019-09-10

    申请号：US15595353

    申请日：2017-05-15

    Applicant: GEN-PROBE INCORPORATED

    Inventor： Steven T. Brentano ,  Dmitry Lyakhov ,  James D. Carlson ,  Norman C. Nelson ,  Lyle J. Arnold ,  Michael M. Becker

    IPC: C12Q1/68 ,  C12Q1/6881 ,  C12Q1/6844 ,  C12Q1/6834 ,  C12Q1/6865

    Abstract: Compositions that are used in nucleic acid amplification in vitro are disclosed, which include a target specific universal (TSU) promoter primer or promoter provider oligonucleotide that includes a target specific (TS) sequence that hybridizes specifically to a target sequence that is amplified and a universal (U) sequence that is introduced into the sequence that is amplified, by using a primer for the universal sequence. Methods of nucleic acid amplification in vitro are disclosed that use one or more TSU oligonucleotides to attached a U sequence to a target nucleic acid in a target capture step and then use a primer for a U sequence in subsequent amplification steps performed in substantially isothermal conditions to make amplification products that contain a U sequence that indicates the presence of the target nucleic acid in a sample.

    SYSTEM AND METHOD OF USING MULTI-CHAMBERED RECEPTACLES
    28.
    发明申请
    SYSTEM AND METHOD OF USING MULTI-CHAMBERED RECEPTACLES 审中-公开

    公开(公告)号：US20170218431A1

    公开(公告)日：2017-08-03

    申请号：US15485330

    申请日：2017-04-12

    Applicant: GEN-PROBE INCORPORATED ,  QUALIGEN, INC.

    Inventor： Scott S. Breidenthal ,  Richard S. Lee ,  Norman C. Nelson ,  Matthew J. Scott ,  Jason A. Taylor

    IPC: C12Q1/68 ,  B01L3/00 ,  B01F3/12 ,  B01F11/00 ,  B01F15/00

    Abstract: A receptacle having a plurality of interconnected chambers arranged to permit multiple process steps or processes to be performed independently or simultaneously. The receptacles are manufactured to separate liquid from dried reagents and to maintain the stability of the dried reagents. An immiscible liquid, such as an oil, is included to control loading of process materials, facilitate mixing and reconstitution of dried reagents, limit evaporation, control heating of reaction materials, concentrate solid support materials to prevent clogging of fluid connections, provide minimum volumes for fluid transfers, and to prevent process materials from sticking to chamber surfaces. The receptacles can be adapted for use in systems having a processing instrument that includes an actuator system for selectively moving fluid substances between chambers and a detector. The actuator system can be arranged to concentrate an analyte present in a sample. The detector can be used to detect an optical signal emitted by the contents of the receptacle.

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