公开(公告)号：US20070170059A1

公开(公告)日：2007-07-26

申请号：US11536319

申请日：2006-09-28

申请人： Jeong Lee ,  Hye Cho ,  Nam Huh ,  Sung Kim

发明人： Jeong Lee ,  Hye Cho ,  Nam Huh ,  Sung Kim

IPC分类号： C07K1/26

CPC分类号： B01L3/502792 ,  B01L2300/089 ,  B01L2300/161 ,  B01L2400/0427 ,  F04B19/006

摘要： A method for controlling the hydrophilic droplet using the electrowetting principle is disclosed. More specifically, the present invention provides a method for increasing the change of the contact angle in the interface that is formed between the hydrophilic droplet and the nonpolar fluid by adding a constant concentration of two-element electrolyte or three-element electrolyte to the hydrophilic droplet; and an apparatus for controlling the droplet having the change of the contact angle and velocity scope increased by the method.

摘要翻译： 公开了一种使用电润湿原理控制亲水性液滴的方法。 更具体地说，本发明提供了一种通过向亲水液滴中加入恒定浓度的双元素电解质或三元素电解质来增加形成在亲水性液滴和非极性流体之间的界面中的接触角变化的方法 ; 并且通过该方法增加了具有接触角和速度范围的变化的液滴的控制装置。

公开(公告)号：US20070148763A1

公开(公告)日：2007-06-28

申请号：US11491825

申请日：2006-07-24

申请人： Nam Huh ,  Jeong-gun Lee

发明人： Nam Huh ,  Jeong-gun Lee

IPC分类号： C12M3/00

CPC分类号： B01L3/0241 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2200/143 ,  B01L2300/0819 ,  B01L2300/089 ,  B01L2400/027 ,  B01L2400/0427 ,  G01N2035/00237 ,  Y10T436/2575

摘要： Provided is a quantitative cell dispensing apparatus using liquid droplet manipulation, for quantitatively dispensing cells from hydrophilic suspension containing a plurality of cells. The quantitative cell dispensing apparatus includes hydrophilic suspension, a liquid droplet manipulation unit, a dispensing unit, a sensing unit, and a control unit. The hydrophilic suspension includes a plurality of cells and electrolyte, and the liquid droplet manipulation unit. The liquid droplet manipulation unit includes an inlet and a liquid droplet outlet portion. The dispensing unit discharges a liquid droplet received from the liquid droplet outlet portion to a target portion. The sensing unit optically senses liquid droplets distributed on a panel of the liquid droplet manipulation unit. The control unit analyses the number of cells contained in each liquid droplet using a single sensed by the sensing unit, and controls the liquid droplet manipulation unit to transfer a liquid droplet containing a predetermined number of cells to the liquid droplet outlet portion.

摘要翻译： 提供了一种使用液滴操作的定量细胞分配装置，用于从含有多个细胞的亲水性悬浮液定量分配细胞。 定量细胞分配装置包括亲水性悬浮液，液滴操作单元，分配单元，感测单元和控制单元。 亲水性悬浮液包括多个细胞和电解质，以及液滴操作单元。 液滴操作单元包括入口和液滴出口部分。 分配单元将从液滴出口部分接收的液滴排放到目标部分。 感测单元光学地感测分布在液滴操作单元的面板上的液滴。 控制单元使用感测单元检测到的单个分析每个液滴中包含的细胞的数量，并且控制液滴操作单元将含有预定数量的细胞的液滴转移到液滴出口部分。

公开(公告)号：US20070035587A1

公开(公告)日：2007-02-15

申请号：US11503721

申请日：2006-08-14

申请人： Jeong-gun Lee ,  Hye-jung Cho ,  Nam Huh ,  In-seok Kang ,  Beom-seok Lee

发明人： Jeong-gun Lee ,  Hye-jung Cho ,  Nam Huh ,  In-seok Kang ,  Beom-seok Lee

IPC分类号： B41J2/06

CPC分类号： B01L3/0262 ,  B01J19/0046 ,  B01J2219/00364 ,  B01J2219/00497 ,  B01J2219/00527 ,  B01J2219/00574 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00689 ,  B01J2219/00691 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00729 ,  B01J2219/00743 ,  B01L2300/0838 ,  B01L2400/027

摘要： A device and method for printing biomolecules on a substrate uses an electric charge concentration effect. The device overcomes limitations of the material and surface characteristics of a substrate, enables accurate dropping of a biomolecular droplet onto a target surface of the substrate, prevents electric discharge, and thus allows the manufacturing of a high density biochip by depositing numerous biomolecular droplets, which are small in size and volume, onto a substrate. The device includes: a needle-shaped electric field forming electrode; a substrate which is grounded and disposed below the electric field forming electrode, the substrate including a target surface; and an open circuit type voltage applying unit which supplies first electric charges to the electric field forming electrode to drop the biomolecular droplet onto the target surface of the substrate.

摘要翻译： 用于在基板上印刷生物分子的装置和方法使用电荷浓度效应。 该器件克服了衬底的材料和表面特性的局限性，能够将生物分子液滴精确地滴落到衬底的靶表面上，防止放电，从而允许通过沉积许多生物分子液滴来制造高密度生物芯片， 尺寸和体积小到衬底上。 该装置包括：针状电场形成电极; 基板，其接地并设置在所述电场形成电极下方，所述基板包括目标表面; 以及开路型电压施加单元，其向所述电场形成电极提供第一电荷，以将所述生物分子液滴落到所述基板的所述目标表面上。

公开(公告)号：US20050181407A1

公开(公告)日：2005-08-18

申请号：US11052345

申请日：2005-02-07

申请人： Nam Huh ,  Jong-myeon Park

发明人： Nam Huh ,  Jong-myeon Park

IPC分类号： C12Q1/68 ,  B05D3/00 ,  C12M1/00 ,  C12M1/34 ,  C12N15/09 ,  C40B60/14 ,  G01N33/53 ,  G01N33/543 ,  G01N33/547 ,  G01N37/00

CPC分类号： C12Q1/6837 ,  B01J2219/00387 ,  B01J2219/00529 ,  B01J2219/00596 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00677 ,  C40B60/14 ,  G01N33/54393

摘要： Provided is a method for noncovalently immobilizing a biomolecule on a solid substrate, including: providing a solid substrate having a first functional group attached thereto, the first functional group having a hydrogen bond donating ability; and reacting a mixture of a compound having a hydrogen bond accepting ability and a biomolecule functionalized with a second functional group, with the surface of the substrate, the second functional group having a hydrogen bond donating ability, in order to noncovalently immobilize the biomolecule on the substrate.

摘要翻译： 本发明提供一种在固体基质上非共价固定生物分子的方法，包括：提供具有第一官能团的固体基质，具有氢键赋予能力的第一官能团; 并使具有氢键接受能力的化合物和用第二官能团官能化的生物分子的混合物与底物的表面，第二官能团具有氢键供体能力反应，以将生物分子非常固定地固定在 基质。

公开(公告)号：US20050064488A1

公开(公告)日：2005-03-24

申请号：US10945245

申请日：2004-09-20

申请人： Nam Huh ,  Sung-young Jeong ,  Kui-hyun Kim

发明人： Nam Huh ,  Sung-young Jeong ,  Kui-hyun Kim

IPC分类号： C12P19/34 ,  C12Q1/68

CPC分类号： C12Q1/6853 ,  C12Q2537/143 ,  C12Q2537/125 ,  C12Q2525/161

摘要： Provided is a method for detecting a target nucleic acid, comprising amplifying a target nucleic acid from a nucleic acid sample using a set of primers comprising at least one primer having a tag sequence at its 5′ end and a target binding sequence at its 3′ end; hybridizing the resulting amplified products with detection probes labeled with a label, the detection probes being capable of specifically binding to the tag sequence; hybridizing the hybridization products with capture probes immobilized on a microarray, the capture probes being capable of specifically binding to the target nucleic acid and not to the tag sequence; and determining the results of hybridization.

摘要翻译： 提供了一种用于检测靶核酸的方法，其包括使用一组引物扩增来自核酸样品的靶核酸，所述引物包含至少一个其5'末端具有标签序列的引物和其3'端的靶结合序列 结束; 将得到的扩增产物与用标记物标记的检测探针杂交，检测探针能够特异性结合标签序列; 将杂交产物与固定在微阵列上的捕获探针杂交，所述捕获探针能够特异性结合靶核酸，而不能与标记序列结合; 并确定杂交结果。

公开(公告)号：US07767069B2

公开(公告)日：2010-08-03

申请号：US11536319

申请日：2006-09-28

申请人： Jeong Gun Lee ,  Hye Jung Cho ,  Nam Huh ,  Sung Jae Kim

发明人： Jeong Gun Lee ,  Hye Jung Cho ,  Nam Huh ,  Sung Jae Kim

IPC分类号： G01N27/447

CPC分类号： B01L3/502792 ,  B01L2300/089 ,  B01L2300/161 ,  B01L2400/0427 ,  F04B19/006

摘要： A method for controlling the hydrophilic droplet using the electrowetting principle is disclosed. More specifically, the present invention provides a method for increasing the change of the contact angle in the interface that is formed between the hydrophilic droplet and the nonpolar fluid by adding a constant concentration of two-element electrolyte or three-element electrolyte to the hydrophilic droplet; and an apparatus for controlling the droplet having the change of the contact angle and velocity scope increased by the method.

摘要翻译： 公开了一种使用电润湿原理控制亲水性液滴的方法。 更具体地说，本发明提供了一种通过向亲水液滴中加入恒定浓度的双元素电解质或三元素电解质来增加形成在亲水性液滴和非极性流体之间的界面中的接触角变化的方法 ; 并且通过该方法增加了具有接触角和速度范围的变化的液滴的控制装置。

公开(公告)号：US07611889B2

公开(公告)日：2009-11-03

申请号：US11052345

申请日：2005-02-07

申请人： Nam Huh ,  Jong-myeon Park

发明人： Nam Huh ,  Jong-myeon Park

IPC分类号： C12M1/34 ,  C12Q1/68 ,  G01N33/53 ,  C07K1/10

CPC分类号： C12Q1/6837 ,  B01J2219/00387 ,  B01J2219/00529 ,  B01J2219/00596 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00677 ,  C40B60/14 ,  G01N33/54393

摘要： Provided is a method for noncovalently immobilizing a biomolecule on a solid substrate, including: providing a solid substrate having a first functional group attached thereto, the first functional group having a hydrogen bond donating ability; and reacting a mixture of a compound having a hydrogen bond accepting ability and a biomolecule functionalized with a second functional group, with the surface of the substrate, the second functional group having a hydrogen bond donating ability, in order to noncovalently immobilize the biomolecule on the substrate.

摘要翻译： 本发明提供一种在固体基质上非共价固定生物分子的方法，包括：提供具有第一官能团的固体基质，具有氢键赋予能力的第一官能团; 并使具有氢键接受能力的化合物和用第二官能团官能化的生物分子的混合物与底物的表面，第二官能团具有氢键供体能力反应，以将生物分子非常固定地固定在 基质。

公开(公告)号：US20090131649A1

公开(公告)日：2009-05-21

申请号：US12110373

申请日：2008-04-28

申请人： Myo-yong LEE ,  Nam Huh ,  Joon-ho Kim

发明人： Myo-yong LEE ,  Nam Huh ,  Joon-ho Kim

IPC分类号： C07H1/00

CPC分类号： C12N15/1006

摘要： Disclosed is a method of separating small RNAs of 200 nucleotides or less from larger RNAs on a solid support, using a kosmotropic salt of different concentrations.

摘要翻译： 公开了一种使用不同浓度的可变性盐分离从固体支持物上的较大RNA分离200个核苷酸或更少的小RNA的方法。

公开(公告)号：US20090048437A1

公开(公告)日：2009-02-19

申请号：US12110391

申请日：2008-04-28

申请人： Myo-yong LEE ,  Nam Huh ,  Joon-ho Kim

发明人： Myo-yong LEE ,  Nam Huh ,  Joon-ho Kim

IPC分类号： C07H21/02

CPC分类号： C07H21/02 ,  C12N15/1003 ,  C12N15/1006

摘要： The present invention provides a method of purifying RNA, including contacting a solid support with an acidic solution having a RNA-containing sample and a kosmotropic salt having a concentration of less than 1M, thereby binding the RNA to the solid support. According to the present invention, RNA is purified efficiently due to high RNA yield and low contamination by DNA. The present invention is particularly effective in purifying RNAs of 200 nucleotides or less.

摘要翻译： 本发明提供了一种纯化RNA的方法，包括使固体支持物与具有含RNA样品的酸性溶液和浓度小于1M的感应性盐接触，从而将RNA与固体支持物结合。 根据本发明，由于高RNA产量和低DNA污染，RNA被有效地纯化。 本发明在纯化200个或更少核苷酸的RNA中特别有效。

公开(公告)号：US20070035567A1

公开(公告)日：2007-02-15

申请号：US11503786

申请日：2006-08-14

申请人： Su-hyeon Kim ,  Jeong-gun Lee ,  Jong-suk Chung ,  Hye-jung Cho ,  Nam Huh ,  In-seok Kang ,  Beom-seok Lee

发明人： Su-hyeon Kim ,  Jeong-gun Lee ,  Jong-suk Chung ,  Hye-jung Cho ,  Nam Huh ,  In-seok Kang ,  Beom-seok Lee

IPC分类号： B41J29/38

CPC分类号： B41J2/06 ,  B01J19/0046 ,  B01J2219/00364 ,  B01J2219/00497 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00689 ,  B01J2219/00691 ,  B01J2219/00693 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00729 ,  B01J2219/00743 ,  B41J2/04535 ,  B41J2/0456 ,  B41J2/04576

摘要： A device for printing a droplet onto a substrate includes: a droplet generating member which is needle-shaped and comprises a receiving portion disposed vertically to receive a solution, and a discharge hole connected to the receiving portion and formed on a bottom of the receiving portion so that the solution can be discharged from the receiving portion; a substrate disposed below the droplet generating member, the substrate includes a target portion to which the droplet discharged from the discharge hole of the droplet generating member is dropped and attached; a voltage applier applying a voltage to the droplet so that the droplet can be dropped onto the target portion of the substrate; a volume measuring unit measuring the volume of the droplet; and a droplet control unit maintaining the volume of the droplet at a predetermined level based on the measured volume of the droplet.

摘要翻译： 用于将液滴印刷到基板上的装置包括：液滴产生构件，其为针状并且包括垂直设置以接收溶液的接收部分，以及连接到接收部分并形成在接收部分的底部上的排出孔 使得溶液可以从接收部分排出; 设置在所述液滴产生部件下方的基板，所述基板包括从所述液滴产生部件的排出孔排出的液滴滴落并附着到的目标部分; 电压施加器，向所述液滴施加电压，使得所述液滴能够落到所述基板的所述目标部分上; 体积测量单元，测量液滴的体积; 以及液滴控制单元，其基于所测量的液滴体积将液滴的体积保持在预定水平。