公开(公告)号：US20070172938A1

公开(公告)日：2007-07-26

申请号：US10591288

申请日：2005-02-28

申请人： Shigeru Deguchi ,  Mikiko Tsudome ,  Susumu Ito ,  Koki Horikoshi

发明人： Shigeru Deguchi ,  Mikiko Tsudome ,  Susumu Ito ,  Koki Horikoshi

IPC分类号： C12N1/20 ,  C08B5/00

CPC分类号： C12N1/22 ,  C08L1/02

摘要： The present invention relates to a solid medium containing as a medium-solidifying component a cellulose gel, in particular a cellulose gel which is a porous cellulose gel structure containing cellulose as the skeletal part and having a cellulose concentration of 0.01% or higher and a porosity of 50% or higher, as well as a process for producing the same. The solid medium of the invention can be obtained by dispersing cellulose in a solvent, especially an aqueous thiocyanate salt solution, stirring and/or heating the dispersion to dissolve the cellulose, subsequently cooling the solution and/or removing the solvent to cause the solution to gel, and permeating nutrients into the resultant cellulose gel. The solid medium usable under a wide range of culture conditions where conventional solid media such as agar medium cannot be used, as well as a method for producing the same is provided.

摘要翻译： 本发明涉及一种固体培养基，其中含有纤维素凝胶，特别是作为纤维素作为骨架部分并且纤维素浓度为0.01％以上的多孔纤维素凝胶结构的纤维素凝胶的纤维素凝胶， 为50％以上，以及其制造方法。 本发明的固体培养基可以通过将纤维素分散在溶剂中，特别是硫氰酸盐水溶液中，搅拌和/或加热分散体以溶解纤维素，随后冷却溶液和/或除去溶剂， 凝胶，并将渗透的营养物质加入所得的纤维素凝胶中。 提供了在不能使用常规固体培养基如琼脂培养基的广泛培养条件下可使用的固体培养基及其制备方法。

公开(公告)号：US4962055A

公开(公告)日：1990-10-09

申请号：US32032

申请日：1987-03-30

申请人： Koki Horikoshi ,  Toshiaki Kudo ,  Chiaki Kato

发明人： Koki Horikoshi ,  Toshiaki Kudo ,  Chiaki Kato

IPC分类号： C12N1/21 ,  C12N1/38 ,  C12N9/24 ,  C12N9/86 ,  C12N15/70

CPC分类号： C12Y302/01032 ,  C12N1/38 ,  C12N15/70 ,  C12N9/2402 ,  C12N9/86 ,  C12Y302/01008 ,  C12Y305/02006 ,  C07K2319/02

摘要： A novel plasmid pEAP2, which was constructed from Bacillus sp. 170 chromosomal DNA carrying the gene for extracellular production of penicillinase and a vector plasmid pMB9. A novel microorganism, Escherichia coli HB101(pEAP2) carrying the plasmid pEAP2 and being capable of extracellular production of penicillinase. The method for cultivation of the microorganism is characterized by culturing it in the medium containing NaCl (or KCl) for 16-48 hours. According to this invention, useful high-molecular substances can be produced in a high yield.

摘要翻译： 一种新的质粒pEAP2，其由芽孢杆菌属 携带青霉素酶细胞外产生基因的170个染色体DNA和载体质粒pMB9。 携带质粒pEAP2并能够细胞外产生青霉素酶的新型微生物大肠杆菌HB101（pEAP2）。 培养微生物的方法的特征在于在含有NaCl（或KCl）的培养基中培养16-48小时。 根据本发明，可以高产率生产有用的高分子物质。

公开(公告)号：US4052262A

公开(公告)日：1977-10-04

申请号：US40771

申请日：1970-05-27

申请人： Koki Horikoshi ,  Yonosuke Ikeda

发明人： Koki Horikoshi ,  Yonosuke Ikeda

IPC分类号： C11D3/386 ,  C12N9/54 ,  C12D13/10 ,  C07G7/02

CPC分类号： C12N9/54 ,  C11D3/386 ,  Y10S435/832

摘要： An alkaline protease is produced by cultivation of novel microorganism strains belonging to the Bacillus genus. The alkaline protease has optimum pH of 11 to 12 and is useful as an additive for detergents.

公开(公告)号：US3956064A

公开(公告)日：1976-05-11

申请号：US512058

申请日：1974-10-04

申请人： Koki Horikoshi ,  Tadahiko Ando ,  Koichi Yoshida

发明人： Koki Horikoshi ,  Tadahiko Ando ,  Koichi Yoshida

IPC分类号： C12N9/16 ,  C12N9/22 ,  C12D13/06 ,  C07G7/02 ,  C12D13/10

CPC分类号： C12N9/22 ,  Y10S435/832 ,  Y10S435/835

摘要： A new deoxyribonuclease (DNase) having preservability and high temperature stability is provided by cultivating a new strain M-26 of the genus Bacillus in an alkaline culture medium. The DNase has such a specificity that it very selectively cleaves phosphodiester bonds between deoxyguanosine and deoxyguanosine in a molecule of deoxyribonucleic acid (DNA) while leaving phosphate groups at the 5'-position.

摘要翻译： 通过在碱性培养基中培养芽孢杆菌属菌株M-26，提供具有保存性和高温稳定性的新脱氧核糖核酸酶（DNase）。 DNase具有这样的特异性，即它在脱氧核糖核酸（DNA）的分子中非常有选择地切割脱氧鸟苷与脱氧鸟苷之间的磷酸二酯键，同时在5'-位上留下磷酸基。

公开(公告)号：US20050202409A1

公开(公告)日：2005-09-15

申请号：US11062833

申请日：2005-02-23

申请人： Hideto Takami ,  Koki Horikoshi ,  Yoshihiro Takaki ,  Gab-Joo Chee ,  Shinro Nishi ,  Shigeru Shimamura ,  Hiroko Suzuki ,  Ikuo Uchiyama ,  Zhijun Li

发明人： Hideto Takami ,  Koki Horikoshi ,  Yoshihiro Takaki ,  Gab-Joo Chee ,  Shinro Nishi ,  Shigeru Shimamura ,  Hiroko Suzuki ,  Ikuo Uchiyama ,  Zhijun Li

IPC分类号： C12Q1/00 ,  G01N33/48 ,  G01N33/50 ,  G06F19/00

CPC分类号： G06F19/24

摘要： The invention relates to a method of judging the thermostability of a protein, comprising the steps of calculating an analytical value specific to a test protein by a principal component analysis based on the amino acid composition of the protein calculated from the data of the amino acid sequence of the protein or the nucleotide sequence of the gene and comparing the analytical value with an analytical value of a protein which is retained by a thermostable organism and corresponds to the test protein, and further relates to a program for allowing a computer to execute processing for judging the thermostability of a protein by the method, and a computer readable recording medium having recorded the program thereon.

摘要翻译： 本发明涉及一种判断蛋白质的热稳定性的方法，包括以下步骤：基于由氨基酸序列的数据计算的蛋白质的氨基酸组成，通过主成分分析计算测试蛋白质特异性的分析值 的蛋白质或该基因的核苷酸序列，并将分析值与由热稳定性生物体保留并对应于测试蛋白质的蛋白质的分析值进行比较，并且还涉及允许计算机执行处理的程序 通过该方法判断蛋白质的热稳定性，以及在其上记录有程序的计算机可读记录介质。

公开(公告)号：US5705378A

公开(公告)日：1998-01-06

申请号：US528923

申请日：1995-09-15

申请人： Masahiro Yoshida ,  Nobuyuki Nakamura ,  Koki Horikoshi

发明人： Masahiro Yoshida ,  Nobuyuki Nakamura ,  Koki Horikoshi

IPC分类号： C12N9/10 ,  C12P19/12 ,  C12N9/12 ,  C12N1/12 ,  C12N9/24

CPC分类号： C12N9/1051 ,  C12P19/12 ,  C12R1/01

摘要： Disclosed are a novel microorganism (FERM BP-5144) belonging to the genus Plesiomonas and having ability to produce maltose phosphorylase and trehalose phosphorylase required for the enzymatic production of trehalose and novel maltose phosphorylase and trehalose phosphorylase obtainable from the microorganism as well as a process for producing the enzymes. A novel process for enzymatically producing trehalose (O-.alpha.-D-glucopyranosyl-(1.fwdarw.1)-D-glucopyranoside) is also disclosed.

公开(公告)号：US5518726A

公开(公告)日：1996-05-21

申请号：US242853

申请日：1994-05-16

申请人： Kazuhito Moriya ,  Koki Horikoshi

发明人： Kazuhito Moriya ,  Koki Horikoshi

IPC分类号： C02F1/40 ,  C02F3/00 ,  C02F3/34 ,  C12N1/20 ,  C12R1/20 ,  E02B15/10 ,  A01N63/00 ,  C12N1/00 ,  C12N1/26

CPC分类号： C12R1/20 ,  C02F3/34 ,  Y10S435/85

摘要： A microorganism belonging to the genus Flavobacterium posessing the capacity to decompose hydrocarbons, tolerance to sulfurous acids, tolerance to salinity, tolerance to organic solvents, and tolerance to pressure. The microorganism is of a strain of the genus Flavobacterium DS-711 (FERM BP-4010). A hydrocarbon emulsifier or solubilizer having as an active component thereof a water soluble and acetone insoluble fraction obtained by culturing DS-711 strain. In addition the emulsifier or solubilizer obtained by culturing the strain DS-711 contains 18.4% protein, 18.8% carbohydrates and 28.6% lipids. A separation method for an organic-solvent tolerant microorganism, wherein a sample is mixed with water and an organic solvent, shaking culturing is conducted, a cultured mixture is allowed to stand, separation into an aqueous phase and an organic solvent phase is conducted, an appropriate amount of said organic solvent phase is added to a culture medium and cultured, and microorganisms which grow therein are isolated.

摘要翻译： 属于属于分解碳氢化合物的能力的微生物的微生物，对亚硫酸的耐受性，对盐度的耐受性，对有机溶剂的耐受性和耐受性。 该微生物是属于属于黄杆菌属DS-711（FERM BP-4010）的菌株。 具有作为其活性成分的烃乳化剂或增溶剂是通过培养DS-711菌株获得的水溶性和丙酮不溶性级分。 此外，通过培养菌株DS-711获得的乳化剂或增溶剂含有18.4％的蛋白质，18.8％的碳水化合物和28.6％的脂质。 一种有机溶剂耐受微生物的分离方法，其中样品与水和有机溶剂混合，进行振荡培养，使培养的混合物静置，分离成水相，并进行有机溶剂相， 将适量的所述有机溶剂相加入到培养基中并进行培养，分离生长在其中的微生物。

公开(公告)号：US5190874A

公开(公告)日：1993-03-02

申请号：US401507

申请日：1989-08-30

申请人： Koki Horikoshi

发明人： Koki Horikoshi

IPC分类号： C12N1/21 ,  C12N9/24 ,  C12N9/86 ,  C12N15/55 ,  C12N15/70

CPC分类号： C12Y302/01032 ,  C12N15/70 ,  C12N9/248 ,  C12N9/86 ,  C12Y302/01008 ,  C12Y305/02006 ,  C07K2319/02

摘要： A novel plasmid pXP102-3 which is constructed from Aeromonas sp. No. 212 chromosomal DNA fragment coding for production of xylanase and a vector plasmid pEAP2 carrying a DNA fragment coding for production and secretion of penicillinase. A novel microorganism, Escherichia coli HB101 (pXP102-3), containing the plasmid pXP102-3 and being capable of producing and secreting penicillinase and xylanase. A method for cultivation of the microorganism characterized in that it is cultured in a broth containing NaCl for 12 to 48 hours.

摘要翻译： 一种新型质粒pXP102-3，其由气单胞菌属 编码木聚糖酶的212号染色体DNA片段和携带编码青霉素酶生产和分泌的DNA片段的载体质粒pEAP2。 一种新型微生物，大肠杆菌HB101（pXP102-3），其含有质粒pXP102-3，能够产生和分泌青霉素酶和木聚糖酶。 一种培养微生物的方法，其特征在于将其在含有NaCl的肉汤中培养12至48小时。

公开(公告)号：US4945049A

公开(公告)日：1990-07-31

申请号：US343263

申请日：1989-04-14

申请人： Toru Hamaya ,  Koki Horikoshi

发明人： Toru Hamaya ,  Koki Horikoshi

IPC分类号： B03C1/01 ,  C01G49/06 ,  C01G49/08 ,  C12P3/00 ,  C22B3/18 ,  G11B5/706 ,  G11B5/714 ,  H01F1/44

CPC分类号： B82Y30/00 ,  B03C1/01 ,  C01G49/06 ,  C01G49/08 ,  C12P3/00 ,  C22B3/18 ,  G11B5/70642 ,  G11B5/714 ,  H01F1/445 ,  C01P2002/72 ,  C01P2004/03 ,  C01P2004/52 ,  C01P2004/62 ,  C01P2004/64 ,  C01P2004/82 ,  Y02P10/234

摘要： The present invention relates to a method for preparing magnetic powder comprising homogeneous and fine particles using an alkali-producing enzyme. The object of the present invention is to provide a method suitable for preparing magnetic powder comprising relatively small particles, for instance, fine particles having a particle size ranging from 50 to 500 nm. The present invention relates to a method for preparing at least one member selected from the group consisting of iron oxides, iron hydroxides and iron oxyhydroxides which comprises the step of alkalizing a solution containing iron ions utilizing an alkali-producing enzyme and a substrate of the enzyme.According to the present invention, there can be produced magnetite (Fe.sub.3 O.sub.4) and maghemite (gamma-Fe.sub.2 O.sub.3) useful as magnetic powder as well as goethite (alpha-FeO(OH)), hematite (alpha-Fe.sub.2 O.sub.3) and lepidocrocite (gamma-FeO(OH)) useful as the starting materials thereof. The magnetic powder can be used as the materials for magnetic recording and magnetic fluid; carriers for bioreactors; those for magnetic separation of cells and biopolymers; and those for microcarriers of medicines.

摘要翻译： PCT No.PCT / JP88 / 00814 Sec。 371日期：1989年4月14日 102（e）日期1989年4月14日PCT PCT 1988年8月18日PCT公布。 出版物WO89 / 01521 日本特开1989年2月23日。本发明涉及一种使用碱生产酶制备含有均匀和细颗粒的磁粉的方法。 本发明的目的是提供一种适用于制备包含相对小的颗粒的磁粉的方法，例如粒度范围为50-500nm的细颗粒。 本发明涉及一种制备选自铁氧化物，氢氧化铁和羟基氧化铁中的至少一种的方法，该方法包括使用碱产生酶和酶底物使含有铁离子的溶液碱化的步骤 。 根据本发明，可以制造用作磁性粉末的磁铁矿（Fe 3 O 4）和磁赤铁矿（γ-Fe 2 O 3）以及针铁矿（α-FeO​​（OH）），赤铁矿（α-Fe 2 O 3）和氯铁矿（γ-FeO （OH））作为起始原料。 磁粉可用作磁记录和磁性液体的材料; 生物反应器的载体; 用于细胞和生物聚合物的磁分离的那些; 和那些用于药物微载体的药物。

公开(公告)号：US4624922A

公开(公告)日：1986-11-25

申请号：US590636

申请日：1984-03-19

申请人： Koki Horikoshi ,  Toshiaki Kudo ,  Hiroshi Honda

发明人： Koki Horikoshi ,  Toshiaki Kudo ,  Hiroshi Honda

IPC分类号： C12N1/38 ,  C12N9/24 ,  C12N9/86 ,  C12N15/67 ,  C12N15/70 ,  C12N15/00 ,  C12N1/00 ,  C12N1/20 ,  C12P19/34

CPC分类号： C12Y302/01032 ,  C12N1/38 ,  C12N15/67 ,  C12N15/70 ,  C12N9/248 ,  C12N9/86 ,  C12Y302/01008 ,  C07K2319/02

摘要： A novel plasmid pCX311, which was constructed from Bacillus sp. C125 chromosomal DNA carrying the gene for extracellular production of xylanase and a vector plasmid pBR322. A novel microorganism, Escherichia coli HB101 (pCX311) carrying the plasmid pCX311 and being capable of extracellular production of xylanase. Method for culturing the microorganism characterized by cultivation of it in the medium containing NaCl (or KCl) and bran, or NaCl (or KCl) and xylan for 12-48 hours. According to this invention, useful high-molecular substances can be produced in a high yield.

摘要翻译： 一种新型的质粒pCX311，由Bacillus sp。 携带用于细胞外产生木聚糖酶的基因的C125染色体DNA和载体质粒pBR322。 携带质粒pCX311并能够细胞外产生木聚糖酶的新型微生物大肠杆菌HB101（pCX311）。 培养微生物的方法，其特征在于在含有NaCl（或KCl）和麸，或NaCl（或KCl）和木聚糖的培养基中培养12-48小时。 根据本发明，可以高产率生产有用的高分子物质。