公开(公告)号：US20150259737A1

公开(公告)日：2015-09-17

申请号：US14729376

申请日：2015-06-03

Applicant: Illumina, Inc.

Inventor： Kay Klausing ,  Min-Jui Richard Shen ,  John Moore ,  Vincent Peter Smith ,  Kevin Hall

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12N15/1003 ,  C12Q1/6832 ,  C12Q1/6869 ,  C40B20/04 ,  C12Q2523/319 ,  C12Q2527/125 ,  C12Q2527/127

Abstract: A method of inhibiting light-induced degradation of nucleic acids includes irradiating a portion of the nucleic acids in the presence of a detection solution comprising a polyphenolic compound. A method of detecting a nucleic acid having a fluorescent tag includes irradiating at least a portion of the nucleic acid with light of a suitable wavelength to induce a fluorescence emission and detecting the fluorescence emission. Optionally, the polyphenolic compound is gallic acid, a lower alkyl ester thereof, or mixtures thereof. A kit includes one or more nucleotides, an enzyme capable of catalyzing incorporation of the nucleotides into a nucleic acid strand and a polyphenolic compound suitable for preparing a detection solution.

Abstract translation: 抑制光诱导的核酸降解的方法包括在存在包含多酚化合物的检测溶液的情况下照射一部分核酸。 检测具有荧光标签的核酸的方法包括用合适波长的光照射核酸的至少一部分以诱导荧光发射并检测荧光发射。 任选地，多酚化合物是没食子酸，其低级烷基酯或其混合物。 试剂盒包括一个或多个核苷酸，能够催化将核苷酸掺入核酸链的酶和适于制备检测溶液的多酚化合物。

公开(公告)号：US12077789B2

公开(公告)日：2024-09-03

申请号：US17829866

申请日：2022-06-01

Applicant: ILLUMINA, INC.

Inventor： Misha Golynskiy ,  Mariko Matsuura ,  Saurabh Nirantar ,  Hamed Tabatabaei Ghomi ,  Seth McDonald ,  Ryan Craig ,  Sergio Peisajovich ,  Kyrie Johnson ,  Kay Klausing ,  Eric Murtfeldt ,  Alexandra Exner

IPC: C12N9/12 ,  C12Q1/6874

CPC classification number: C12N9/1252 ,  C12Q1/6874 ,  C12Y207/07007

Abstract: Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain low pre-phasing rates when using ambiently stored polymerases, as well as methods and kits using the same.

公开(公告)号：US20240102067A1

公开(公告)日：2024-03-28

申请号：US18473971

申请日：2023-09-25

Applicant: Illumina, Inc.

Inventor： Kay Klausing ,  Jonathan Boutell ,  Trina Osothprarop ,  Oliver Miller ,  Justin Robbins

IPC: C12P19/34 ,  C12Q1/6874

CPC classification number: C12P19/34 ,  C12Q1/6874

Abstract: This disclosure relates to novel mjresynthesis kits and methods, in particular for use in pairwise sequencing.

公开(公告)号：US11426723B2

公开(公告)日：2022-08-30

申请号：US16584353

申请日：2019-09-26

Applicant: Illumina, Inc.

Inventor： Wesley A. Cox-Muranami ,  Kay Klausing ,  Bradley Kent Drews ,  Nicholas Watson ,  Jennifer Olivia Foley ,  Murphy Hitchcock ,  Paul Sangiorgio ,  Sz-Chin Steven Lin

IPC: B01L3/00

Abstract: A system includes a fluidic device, a flow control valve, a first reagent fluid reservoir fluidly connectable to the fluidic device by the flow control valve, a first fluid buffer reservoir fluidly connectable to the fluidic device by the flow control valve, and a common fluid buffer source fluidly connectable to the fluidic device by the flow control valve. The flow control valve permits flow comprising: (i) flow from the first reagent fluid reservoir to the fluidic device, (ii) flow from the common fluid buffer source to the fluidic device, (iii) flow from the fluidic device to the first fluid buffer reservoir, (iv) flow from the first reagent fluid reservoir to the fluidic device, and (v) flow from the first fluid buffer reservoir to the fluidic device.

公开(公告)号：US11001816B2

公开(公告)日：2021-05-11

申请号：US16703569

申请日：2019-12-04

Applicant: Illumina, Inc. ,  Illumina Cambridge Limited ,  Illumina Singapore Pte. Ltd.

Inventor： Kay Klausing ,  Hamed Tabatabaei Ghomi ,  Misha Golynskiy ,  Saurabh Nirantar ,  Seth McDonald ,  Sergio Peisajovich

IPC: C12N9/12

Abstract: Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain high fidelity under reduced incorporation times, as well as methods and kits using the same.

公开(公告)号：US10900077B2

公开(公告)日：2021-01-26

申请号：US16405679

申请日：2019-05-07

Applicant: Illumina, Inc.

Inventor： Robert C. Kain ,  Xiaohai Liu ,  Wenyi Feng ,  Bernard Hirschbein ,  Helmy A. Eltoukhy ,  Xiaolin Wu ,  Geoffrey Paul Smith ,  Jonathan Mark Boutell ,  Thomas Joseph ,  Randall Smith ,  Min-Jui Richard Shen ,  Carolyn Tregidgo ,  Kay Klausing

IPC: C12Q1/68 ,  C12Q1/6874 ,  C12Q1/6869

Abstract: The present disclosure provides methods and systems for detecting multiple different nucleotides in a sample. In particular, the disclosure provides for detection of multiple different nucleotides in a sample utilizing fewer detection moieties than the number of nucleotides being detected and/or fewer imaging events than the number of nucleotides being detected.

公开(公告)号：US20200318167A1

公开(公告)日：2020-10-08

申请号：US16832309

申请日：2020-03-27

Applicant: ILLUMINA, INC.

Inventor： Boyan Boyanov ,  Liangliang Qiang ,  Kevin L. Gunderson ,  Kay Klausing ,  Lea Pickering ,  Cyril Delattre ,  Tarun Khurana

IPC: C12Q1/6818 ,  C12Q1/6874 ,  C12Q1/6816

Abstract: Under one aspect, a composition includes a substrate; a first polynucleotide coupled to the substrate; a second polynucleotide hybridized to the first polynucleotide; and a catalyst coupled to a first nucleotide of the second polynucleotide, the catalyst being operable to cause a chemiluminogenic molecule to emit a photon. Under another aspect, a method includes providing a catalyst operable to cause a first chemiluminogenic molecule to emit a photon; providing a substrate; providing a first polynucleotide coupled to the substrate; hybridizing a second polynucleotide to the first polynucleotide; coupling a first quencher to a first nucleotide of the second polynucleotide; and inhibiting, by the first quencher, photon emission by the first chemiluminogenic molecule.

公开(公告)号：US20200181587A1

公开(公告)日：2020-06-11

申请号：US16703569

申请日：2019-12-04

Applicant: Illumina, Inc. ,  Illumina Cambridge Limited ,  Illumina Singapore Pte. Ltd.

Inventor： Kay Klausing ,  Hamed Tabatabael Ghomi ,  Misha Golynskiy ,  Saurabh Nirantar ,  Seth McDonald ,  Sergio Peisajovich

IPC: C12N9/12

Abstract: Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain high fidelity under reduced incorporation times, as well as methods and kits using the same.

公开(公告)号：US09856529B2

公开(公告)日：2018-01-02

申请号：US14729376

申请日：2015-06-03

Applicant: Illumina, Inc.

Inventor： Kay Klausing ,  Min-Jui Richard Shen ,  John Moore ,  Vincent Peter Smith ,  Kevin Hall

IPC: C12Q1/68 ,  C40B20/04 ,  C12N15/10 ,  C12Q1/48

CPC classification number: C12Q1/6874 ,  C12N15/1003 ,  C12Q1/6832 ,  C12Q1/6869 ,  C40B20/04 ,  C12Q2523/319 ,  C12Q2527/125 ,  C12Q2527/127

Abstract: A method of inhibiting light-induced degradation of nucleic acids includes irradiating a portion of the nucleic acids in the presence of a detection solution comprising a polyphenolic compound. A method of detecting a nucleic acid having a fluorescent tag includes irradiating at least a portion of the nucleic acid with light of a suitable wavelength to induce a fluorescence emission and detecting the fluorescence emission. Optionally, the polyphenolic compound is gallic acid, a lower alkyl ester thereof, or mixtures thereof. A kit includes one or more nucleotides, an enzyme capable of catalyzing incorporation of the nucleotides into a nucleic acid strand and a polyphenolic compound suitable for preparing a detection solution.

公开(公告)号：US20170356030A1

公开(公告)日：2017-12-14

申请号：US15507465

申请日：2015-09-10

Applicant: ILLUMINA, INC.

Inventor： Boyan Boyanov ,  Liangliang Qiang ,  Kevin L. Gunderson ,  Kay Klausing ,  Lea Pickering ,  Cyril De Lattre ,  Tarun Khurana

IPC: C12Q1/68

CPC classification number: C12Q1/6818 ,  C12Q1/6816 ,  C12Q1/6874 ,  C12Q2533/101 ,  C12Q2535/113 ,  C12Q2535/122 ,  C12Q2563/103 ,  C12Q2563/125

Abstract: Under one aspect, a composition includes a substrate; a first polynucleotide coupled to the substrate; a second polynucleotide hybridized to the first polynucleotide; and a catalyst coupled to a first nucleotide of the second polynucleotide, the catalyst being operable to cause a chemiluminogenic molecule to emit a photon. Under another aspect, a method includes providing a catalyst operable to cause a first chemiluminogenic molecule to emit a photon; providing a substrate; providing a first polynucleotide coupled to the substrate; hybridizing a second polynucleotide to the first polynucleotide; coupling a first quencher to a first nucleotide of the second polynucleotide; and inhibiting, by the first quencher, photon emission by the first chemiluminogenic molecule.