公开(公告)号：US08309367B2

公开(公告)日：2012-11-13

申请号：US11105460

申请日：2005-04-14

申请人： Richard Saul ,  Mark T. Martin

发明人： Richard Saul ,  Mark T. Martin

IPC分类号： G01N33/53

CPC分类号： C12N13/00 ,  B01J19/126 ,  C12N11/00 ,  G01N1/44 ,  G01N33/5436 ,  G01N33/54393 ,  Y10S436/806 ,  Y10T436/115831 ,  Y10T436/116664 ,  Y10T436/24

摘要： The present invention concerns a novel means by which liquids can be moved or mixed. Microwaves strike and heat materials that are highly susceptible to microwave heating. The susceptible materials are on, within, or near materials that melt or change shape in response to temperature increases. Upon microwave irradiation, these materials change shape (e.g., shrink or melt), causing the movement of liquids. The invention is important in many microfluidics applications, especially in biomedical analysis, where it is valuable to be able to move small volumes of liquids (e.g., on a microarray chip).

摘要翻译： 本发明涉及可移动或混合液体的新颖手段。 微波冲击和加热材料，高度易受微波加热。 敏感材料是在温度升高时熔化或变形的材料中，内部或附近。 在微波照射下，这些材料变形（例如，收缩或熔化），导致液体的移动。 本发明在许多微流体应用中是重要的，特别是在生物医学分析中，其能够移动小体积液体（例如，在微阵列芯片上）是有价值的。

公开(公告)号：US06852502B1

公开(公告)日：2005-02-08

申请号：US08467712

申请日：1995-06-06

申请人： Mark T. Martin

发明人： Mark T. Martin

IPC分类号： C12Q1/32 ,  C12Q1/00 ,  G01N21/66 ,  G01N21/76 ,  G01N21/78 ,  G01N27/327 ,  G01N27/42 ,  G01N33/535 ,  C12Q1/26

CPC分类号： G01N33/535 ,  C12Q1/005 ,  G01N21/66 ,  G01N21/76

摘要： Electrochemiluminescent enzymes, their preparation and use as biosensors are disclosed. Specifically, two appendages are covalently attached to a desired dehydrogenase enzyme; (1) a nicotinamide adenine cofactor or analog thereof, and (2) a luminescent ruthenium complex. For example, glucose concentrations is the following way. A doubly-modified glucose dehydrogenase could oxidize glucose with concomitant reduction of the attached NAD+ to NADH. Because NADH, but not NAD+, is able to interact with surface ruthenium to promote ECL, only enzyme molecules that have reacted with glucose will emit light from their ruthenium label in an ECL instrument. The relative close proximity of NADH and ruthenium on the enzyme surface enhances light emission as compared to the same concentrations in free solution. When NADH reduces ruthenium, it returns to become NAD+, permitting multiple cycles of ECL light emission from a single enzyme molecule. Such biosensors can be used in solution or bound to a solid surface. Assays employing the biosensor molecules can be performed on an IGEN OrigenR Analyzer.

摘要翻译： 公开了电化学发光酶，其制备和用作生物传感器。 具体地，两个附属物共价连接到所需的脱氢酶; （1）烟酰胺腺嘌呤辅因子或其类似物，和（2）发光钌络合物。 例如，葡萄糖浓度如下。 双重修饰的葡萄糖脱氢酶可以使附着的NAD +还原成NADH而氧化葡萄糖。 因为NADH而不是NAD +能够与表面钌相互作用以促进ECL，所以只有与葡萄糖反应的酶分子才会在ECL仪器中从钌标记物中发出光。 与游离溶液中相同的浓度相比，NADH和钌在酶表面上的相对接近增强了光发射。 当NADH还原钌时，它返回成为NAD +，允许来自单个酶分子的ECL发光的多个循环。 这种生物传感器可以在溶液中使用或与固体表面结合。 使用生物传感器分子的测定可以在IGEN Origen分析仪上进行。

公开(公告)号：US06524865B1

公开(公告)日：2003-02-25

申请号：US08928075

申请日：1997-09-11

申请人： Mark T. Martin ,  Rick Saul ,  Pam Liang

发明人： Mark T. Martin ,  Rick Saul ,  Pam Liang

IPC分类号： G01N2176

CPC分类号： G01N33/535 ,  C07F15/0053 ,  C07K16/44 ,  G01N33/533

摘要： Electrochemiluminescent-labels and enzyme substrates, which preferably are conjugated, are used in immunoassays and electrochemiluminescence is generated catalytically. In conventional electrochemiluminescence immunoassays, an anti-analyte antibody molecule can give rise to typically 6-8 electrochemiluminescence-active ruthenium atoms, while in the present invention, each enzyme-labeled anti-analyte molecule can give rise to thousands of electrochemiluminescence-active ruthenium atoms per second. An exemplary immunoassay is based on a catalytic process employing &bgr;-lactamase-conjugated anti-analytes which enzymatically hydrolyze electrochemiluminescent-labeled substrates, making them strongly electrochemiluminescent. The electrochemiluminescence signal generated by each anti-analyte molecule (i.e., each analyte molecule) is much greater than with the conventional method. Accordingly, greater sensitivity can be gained in the measurement of low concentrations of a given immunoassay analyte.

摘要翻译： 优选结合的电致化学发光标记和酶底物用于免疫测定，并且催化产生电化学发光。 在常规的电化学发光免疫测定中，抗分析物抗体分子可以产生典型的6-8个电化学发光 - 活性钌原子，而在本发明中，每个酶标记的抗分析物分子可产生数千个电化学发光 - 活性钌原子 每秒。 示例性的免疫测定是基于使用β-内酰胺酶缀合的抗分析物的催化方法，其中酶促水解电化学发光标记的底物，使其具有强电化学发光的功能。 由每个抗分析物分子（即每个分析物分子）产生的电化学发光信号比常规方法大得多。 因此，在给定免疫测定分析物的低浓度的测量中可以获得更高的灵敏度。

公开(公告)号：US07018802B2

公开(公告)日：2006-03-28

申请号：US10234874

申请日：2002-09-04

申请人： Mark T. Martin ,  Rick Saul ,  Pam Liang

发明人： Mark T. Martin ,  Rick Saul ,  Pam Liang

IPC分类号： G01N33/53 ,  C12Q1/34 ,  C12N9/86 ,  C07D499/00

CPC分类号： G01N33/535 ,  C07F15/0053 ,  C07K16/44 ,  G01N33/533

摘要： Electrochemiluminescent-labels and enzyme substrates, which preferably are conjugated, are used in immunoassays and electrochemiluminescence is generated catalytically. In conventional electrochemiluminescence immunoassays, an anti-analyte antibody molecule can give rise to typically 6–8 electrochemiluminescence-active ruthenium atoms, while in the present invention, each enzyme-labeled anti-analyte molecule can give rise to thousands of electrochemiluminescence-active ruthenium atoms per second. An exemplary immunoassay is based on a catalytic process employing β-lactamase-conjugated anti-analytes which enzymatically hydrolyze electrochemiluminescent-labeled substrates, making them strongly electrochemiluminescent. The electrochemiluminescence signal generated by each anti-analyte molecule (i.e., each analyte molecule) is much greater than with the conventional method. Accordingly, greater sensitivity can be gained in the measurement of low concentrations of a given immunoassay analyte.

公开(公告)号：US5643713A

公开(公告)日：1997-07-01

申请号：US485419

申请日：1995-06-07

申请人： Pam Liang ,  Mark T. Martin ,  Liwen Dong

发明人： Pam Liang ,  Mark T. Martin ,  Liwen Dong

IPC分类号： C07D499/26 ,  C12Q1/34 ,  G01N21/78 ,  G01N27/327 ,  G01N27/42 ,  C12Q1/00 ,  G01N33/53 ,  G01N33/554

CPC分类号： C12Q1/34 ,  G01N2333/986 ,  G01N2415/00 ,  G01N2458/30 ,  G01N2458/40

摘要： Detectable compounds comprising a chemically-transformable first compound covalently linked to an electrochemiluminescent compound are provided. Such compounds are useful in processes and kits that monitor the status of the first compound and derive information from such monitoring.

摘要翻译： 提供了包含与电化学发光化合物共价连接的可化学转化的第一化合物的可检测化合物。 这些化合物可用于监测第一化合物的状态并从这种监测中导出信息的方法和试剂盒。

公开(公告)号：US5631137A

公开(公告)日：1997-05-20

申请号：US377495

申请日：1995-01-24

申请人： Mark T. Martin ,  Rodger G. Smith ,  Michael J. Darsley ,  David M. Simpson ,  Gary F. Blackburn

发明人： Mark T. Martin ,  Rodger G. Smith ,  Michael J. Darsley ,  David M. Simpson ,  Gary F. Blackburn

IPC分类号： G01N33/53 ,  B01J20/281 ,  C12N5/10 ,  C12N7/02 ,  C12N15/10 ,  C12Q1/02 ,  C12Q1/68 ,  C12Q1/70 ,  C40B40/02 ,  G01N30/88 ,  G01N33/569 ,  C12Q1/25 ,  C12N9/00

CPC分类号： C40B40/02 ,  C12N15/1037 ,  C12Q1/02 ,  C12Q1/6811 ,  C12Q1/70 ,  G01N33/56983

摘要： Disclosed and claimed are methods for selecting a recombinant virus, phage or cell expressing a catalytic antibody or catalytic portion thereof, or for selecting catalytic activity by a moiety. The method employs reaction-based selection for catalytic activity. The method can also be used to concentrate (increase the proportion of catalytic to non-catalytic moieties) a sample containing a catalytic moiety or viruses, phages or cells expressing a catalytic moiety. The selection or concentrating can be by employing a mechanism-based inhibitor, catalysis-accelerated movement, surface binding, changes in enthalpic component of binding as a function of temperature, or changes in binding by competition, or combinations thereof. The invention also comprehends a method for producing a recombinant virus or a cell-line expressing a catalytic moiety such as a catalytic antibody or catalytic portion thereof; and, this method can include infecting a suitable host with viruses which are screened for the expression. In addition, recombinant viruses and cell-lines so expressing a catalytic moiety such as a catalytic antibody or catalytic portion thereof are also disclosed and claimed.

摘要翻译： 公开并要求的是选择重组病毒，表达催化抗体或其催化部分的噬菌体或细胞，或用于通过部分选择催化活性的方法。 该方法采用基于反应的催化活性选择。 该方法还可用于浓缩（增加非催化部分的催化剂比例）含有催化部分或表达催化部分的病毒，噬菌体或细胞的样品。 选择或浓缩可以通过采用基于机理的抑制剂，催化加速运动，表面结合，作为温度的函数的结合焓分量的变化，或通过竞争的结合变化，或其组合。 本发明还包括生产重组病毒或表达催化部分如催化抗体或其催化部分的细胞系的方法; 并且该方法可以包括用筛选表达的病毒感染合适的宿主。 此外，还公开和要求保护了重组病毒和表达催化部分如催化抗体或其催化部分的细胞系。

公开(公告)号：US20120165209A1

公开(公告)日：2012-06-28

申请号：US13341109

申请日：2011-12-30

申请人： Mark T. Martin

发明人： Mark T. Martin

IPC分类号： G01N21/76 ,  G01N21/64 ,  C40B30/04

CPC分类号： C12N11/00 ,  C12N13/00 ,  G01N33/5436 ,  G01N33/54393

摘要： The present invention concerns a novel means by which chemical preparations can be made. Reactions can be accelerated on special cartridges using microwave energy. The chips contain materials that efficiently absorb microwave energy causing chemical reaction rate increases. The invention is important in many chemical transformations including those used in protein chemistry, in nucleic acid chemistry, in analytical chemistry, and in the polymerase chain reaction.

摘要翻译： 本发明涉及可以制备化学制剂的新型方法。 使用微波能量的特殊墨盒可以加速反应。 这些芯片含有有效吸收微波能量的材料，导致化学反应速率增加。 本发明在许多化学转化中是重要的，包括在蛋白质化学，核酸化学，分析化学和聚合酶链反应中使用的化学转化。

公开(公告)号：US07169894B2

公开(公告)日：2007-01-30

申请号：US10286796

申请日：2002-11-04

申请人： Mark T. Martin

发明人： Mark T. Martin

IPC分类号： C07K1/00 ,  C07K14/00 ,  C07K17/00 ,  G01N33/53 ,  C07H21/04

CPC分类号： G01N33/6803

摘要： A process is disclosed by which a polynucleotide is directly synthesized from the peptide or protein that it encodes without the need for sequencing (or sequence analysis) of the peptide or protein. Information contained in the sequence of the peptide or protein is directly coupled, by the process of reverse translation, to the synthesis of the polynucleotide. The usefulness of reverse translation is that it facilitates the amplification of information held in the amino acid sequence (the primary structure) of an unknown protein or peptide. Amplification is useful for, among other things, the identification and/or scientific investigation of the peptide or protein.

摘要翻译： 公开了一种方法，通过该方法，多核苷酸从其编码的肽或蛋白质直接合成，而不需要肽或蛋白质的测序（或序列分析）。 肽或蛋白质序列中包含的信息通过反向翻译的过程与多核苷酸的合成直接偶联。 反向翻译的有用性是它有助于扩增未知蛋白质或肽的氨基酸序列（一级结构）中保存的信息。 除了别的以外，扩增可用于肽或蛋白质的鉴定和/或科学研究。

公开(公告)号：US06702705B1

公开(公告)日：2004-03-09

申请号：US08392407

申请日：1995-02-22

申请人： Reid von Borstel ,  Jan M. Casadei ,  Balreddy Kamireddy ,  John Kenten ,  Mark T. Martin ,  Richard J. Massey ,  Andrew D. Napper ,  David M. Simpson ,  Rodger Smith ,  Richard C. Titmas ,  Richard O. Williams

发明人： Reid von Borstel ,  Jan M. Casadei ,  Balreddy Kamireddy ,  John Kenten ,  Mark T. Martin ,  Richard J. Massey ,  Andrew D. Napper ,  David M. Simpson ,  Rodger Smith ,  Richard C. Titmas ,  Richard O. Williams

IPC分类号： C12N900

CPC分类号： C07D207/404 ,  C07C229/46 ,  C07C229/48 ,  C07C235/74 ,  C07C271/24 ,  C07C311/06 ,  C07C311/19 ,  C07C317/44 ,  C07C2601/04 ,  C07C2602/42 ,  C07D205/085 ,  C07F9/2408 ,  C07F9/2458 ,  C07F9/301 ,  C07F9/3211 ,  C07F9/4006 ,  C07F9/4075 ,  C07F9/5537 ,  C07F9/650952 ,  C07F9/657181 ,  C07H15/252 ,  C07H19/04 ,  C07K14/55 ,  C07K16/00 ,  C07K16/1063 ,  C07K16/26 ,  C07K16/44 ,  C07K16/468 ,  C07K2319/00 ,  C12N9/0002 ,  C12Q1/25 ,  G01N33/5008 ,  G01N33/531 ,  G01N33/577 ,  G01N2333/16 ,  G01N2333/96472 ,  G01N2500/00

摘要： Catalytic antibodies are disclosed. The catalytic antibodies are specific for prodrug compounds. The catalytic antibodies enhance cleavage of an active drug moiety from a prodrug residue, thereby activating the drug.

摘要翻译： 公开了催化抗体。 催化抗体对前药化合物是特异性的。 催化抗体增强了活性药物部分与前药残留物的切割，从而激活了药物。

公开(公告)号：US06316180B1

公开(公告)日：2001-11-13

申请号：US08880353

申请日：1997-06-23

申请人： Mark T. Martin

发明人： Mark T. Martin

IPC分类号： C12Q100

CPC分类号： C07D277/06 ,  C12Q1/34 ,  G01N2333/986 ,  G01N2415/00

摘要： Detectable compounds comprising a chemically-transformable first compound covalently linked to an electrochemiluminescent compound are provided. Such compounds are useful in processes and kits that monitor the status of the first compound and derive information from such monitoring. A rapid single step assay suitable for the detection or quantification of &bgr;-lactam antibiotics and &bgr;-lactamases. The assay can be performed directly on samples of food, such as milk and meat, blood or serum and is useful in determining the suitability of a particular antibiotic in treating a particular bacterial infection and in diagnosis of a bacterial infection. The assay is also useful in determining and quantifying &bgr;-lactam antibiotic resistance. The assay can be performed on an IGEN OrigenR Analyzer.

摘要翻译： 提供了包含与电化学发光化合物共价连接的可化学转化的第一化合物的可检测化合物。 这些化合物可用于监测第一化合物的状态并从这种监测中获得信息的方法和试剂盒。适用于检测或定量β-内酰胺抗生素和β-内酰胺酶的快速单步测定法。 该测定可以直接对食物样品（例如牛奶和肉，血液或血清）进行，并且可用于确定特定抗生素在治疗特定细菌感染和细菌感染诊断中的适用性。 该测定法也可用于测定和定量β-内酰胺抗生素抗性。 该测定可以在IGEN OrigenR分析仪上进行。