CELL CARRIER, METHODS OF MAKING AND USE
    22.
    发明申请
    CELL CARRIER, METHODS OF MAKING AND USE 有权
    细胞载体,制备和使用方法

    公开(公告)号:US20120156773A1

    公开(公告)日:2012-06-21

    申请号:US13287611

    申请日:2011-11-02

    IPC分类号: C12N5/071 C12N5/077

    摘要: A method of culturing adherent cells is provided, wherein the method comprises providing a carrier for growing the cells, comprising one or more surfaces; and one or more relief features/indentations are present on one or more of the surfaces, wherein the carrier has a length at least about 0.2 mm, a width at least about 0.2 mm, and a height in a range from about 0.012 mm to 0.5 mm; and wherein each of the relief features/indentations has a height above the surfaces in a range from about 2 to 200 μm, and width in a range from about 20 to 200 μm; seeding the cells on the carriers; and growing the cells on the carrier.

    摘要翻译: 提供培养贴壁细胞的方法,其中所述方法包括提供用于生长所述细胞的载体,包含一个或多个表面; 并且在一个或多个表面上存在一个或多个消除特征/凹陷,其中载体具有至少约0.2mm,宽度至少约0.2mm的长度,以及在约0.012mm至0.5的范围内的高度 毫米 并且其中每个所述浮雕特征/压痕的表面高度在约2至200μm的范围内,宽度在约20至200μm的范围内; 将细胞接种在载体上; 并在载体上生长细胞。

    COATED FIBER AND METHOD FOR MAKING THE COATED FIBER
    25.
    发明申请
    COATED FIBER AND METHOD FOR MAKING THE COATED FIBER 审中-公开
    涂覆纤维和制造涂层纤维的方法

    公开(公告)号:US20090075080A1

    公开(公告)日:2009-03-19

    申请号:US11857469

    申请日:2007-09-19

    IPC分类号: B32B5/02

    摘要: A coated fiber comprising an inner fiber comprising an aramid and an outer coating comprising a polyamic acid or a polyetherimide deposited on the inner fiber is provided. The outer coating comprising the polyamic acid may be subjected to curing to form a polyetherimide coating. Also provided are methods for making the coated fiber wherein the coated fiber comprises an inner fiber comprising an aramid and an outer coating comprising a polyamic acid or a polyetherimide.

    摘要翻译: 提供了包含包含芳族聚酰胺的内纤维和包含聚酰胺酸或沉积在内纤维上的聚醚酰亚胺的外涂层的涂覆纤维。 包含聚酰胺酸的外涂层可以进行固化以形成聚醚酰亚胺涂层。 还提供了制备涂覆纤维的方法,其中涂覆的纤维包含包含芳族聚酰胺的内纤维和包含聚酰胺酸或聚醚酰亚胺的外涂层。

    Cell carrier, methods of making and use
    26.
    发明授权
    Cell carrier, methods of making and use 有权
    细胞载体,制备和使用方法

    公开(公告)号:US09453196B2

    公开(公告)日:2016-09-27

    申请号:US13287611

    申请日:2011-11-02

    IPC分类号: C12N5/071 C12N5/00

    摘要: A method of culturing adherent cells is provided, wherein the method comprises providing a carrier for growing the cells, comprising one or more surfaces; and one or more relief features/indentations are present on one or more of the surfaces, wherein the carrier has a length at least about 0.2 mm, a width at least about 0.2 mm, and a height in a range from about 0.012 mm to 0.5 mm; and wherein each of the relief features/indentations has a height above the surfaces in a range from about 2 to 200 μm, and width in a range from about 20 to 200 μm; seeding the cells on the carriers; and growing the cells on the carrier.

    摘要翻译: 提供培养贴壁细胞的方法,其中所述方法包括提供用于生长所述细胞的载体,包含一个或多个表面; 并且在一个或多个表面上存在一个或多个消除特征/凹陷,其中载体具有至少约0.2mm,宽度至少约0.2mm的长度,以及在约0.012mm至0.5的范围内的高度 毫米 并且其中每个所述浮雕特征/压痕的表面高度在约2至200μm的范围内,宽度在约20至200μm的范围内; 将细胞接种在载体上; 并在载体上生长细胞。

    Methods of making cell carrier
    29.
    发明授权
    Methods of making cell carrier 有权
    细胞载体的制备方法

    公开(公告)号:US09453197B2

    公开(公告)日:2016-09-27

    申请号:US13287632

    申请日:2011-11-02

    摘要: A method of making a carrier for growing cells, including providing a polymer film; embossing a patterned surface one or more sides of the polymer film with an embossing roller; generating a pattern of structured indentations on the polymer film; and discretizing the patterned polymer film into a plurality of portions. The embossing pattern generates relief features on the carrier surface. An alternative method of making a carrier is also provided, including extruding a polymer film; embossing a patterned surface on the polymer film with a roller; generating a pattern of structured indentations on the polymer film; imparting a surface treatment to the film; and discretizing the treated polymer film into a plurality of portions.

    摘要翻译: 一种制造用于生长细胞的载体的方法,包括提供聚合物膜; 用压花辊将聚合物膜的一个或多个侧面压花图案化表面; 在聚合物膜上产生结构化凹痕的图案; 并将图案化的聚合物膜离散成多个部分。 压花图案在载体表面上产生浮雕特征。 还提供制备载体的替代方法,包括挤出聚合物膜; 用辊子在聚合物膜上压花图案化表面; 在聚合物膜上产生结构化凹痕的图案; 对薄膜进行表面处理; 并将经处理的聚合物膜离散成多个部分。

    METHODS OF MAKING CELL CARRIER
    30.
    发明申请
    METHODS OF MAKING CELL CARRIER 有权
    制作细胞载体的方法

    公开(公告)号:US20130034669A1

    公开(公告)日:2013-02-07

    申请号:US13287632

    申请日:2011-11-02

    IPC分类号: C23C16/513 B29C59/02

    摘要: A method of making a carrier for growing cells, including providing a polymer film; embossing a patterned surface one or more sides of the polymer film with an embossing roller; generating a pattern of structured indentations on the polymer film; and discretizing the patterned polymer film into a plurality of portions. The embossing pattern generates relief features on the carrier surface. An alternative method of making a carrier is also provided, including extruding a polymer film; embossing a patterned surface on the polymer film with a roller; generating a pattern of structured indentations on the polymer film; imparting a surface treatment to the film; and discretizing the treated polymer film into a plurality of portions.

    摘要翻译: 一种制造用于生长细胞的载体的方法,包括提供聚合物膜; 用压花辊将聚合物膜的一个或多个侧面压花图案化表面; 在聚合物膜上产生结构化凹痕的图案; 并将图案化的聚合物膜离散成多个部分。 压花图案在载体表面上产生浮雕特征。 还提供制备载体的替代方法,包括挤出聚合物膜; 用辊子在聚合物膜上压花图案化表面; 在聚合物膜上产生结构化凹痕的图案; 对薄膜进行表面处理; 并将经处理的聚合物膜离散成多个部分。