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21.发明授权DNA, vectors and transformed hosts encoding Trichoderma reesei endoglucanase I 失效DNA，载体和编码里氏木霉内切葡聚糖酶I的转化宿主
公开(公告)号：US5393670A
公开(公告)日：1995-02-28
申请号：US95253
申请日：1993-07-23
申请人： Jonathan Knowles , Merja Penttila , Tuula Teeri , Helena M. K. Nevalainen , Paivi Lehtovaara-Helenius
发明人： Jonathan Knowles , Merja Penttila , Tuula Teeri , Helena M. K. Nevalainen , Paivi Lehtovaara-Helenius
IPC分类号： C07H20060101 , C07H21/00 , C07K20060101 , C07K7/00 , C12N20060101 , C12N1/00 , C12N1/19 , C12N1/21 , C12N9/00 , C12N9/42 , C12N15/00 , C12N15/56 , C12N15/62 , C12N15/81 , C12N15/52 , C12N15/70
CPC分类号： C12N9/2437 , C12N15/625 , C12N15/81 , C12Y302/01091 , C07K2319/00 , C07K2319/02 , C07K2319/034 , Y10S435/945
摘要： Methods and means for the construction of strains of yeast capable of producing cellulolytic enzymes, achieved by the transfer of chromosomal genes or cDNA copies of mRNAs coding for cellulolytic enzymes isolated from the fungus Trichoderma reesei to yeast cells using recombinant DNA vectors capable of replicating in yeast. The correct expression of these cellulolytic genes in yeast leads to the production of cellulolytic enzymes which are secreted from the cell. This allows the yeast to hydrolyze 3-1,4-glucan substrates such as cellulose.
摘要翻译： 用于构建能够产生纤维素分解酶的酵母菌株的方法和手段，其通过使用能够在酵母中复制的重组DNA载体转染染色体基因或将编码从真菌里氏木霉分离的纤维素分解酶的mRNA的cDNA拷贝到酵母细胞 。 酵母中这些纤维素分解基因的正确表达导致从细胞分泌的纤维素分解酶的产生。 这使得酵母能够水解3-1,4-葡聚糖底物如纤维素。
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公开(公告)号：US09512415B2
公开(公告)日：2016-12-06
申请号：US13701894
申请日：2011-05-30
申请人： Tiina Pakula , Markku Saloheimo , Mari Hakkinen , Ann Westerholm-Parvinen , Merja Penttila , Marika Vitikainen
发明人： Tiina Pakula , Markku Saloheimo , Mari Hakkinen , Ann Westerholm-Parvinen , Merja Penttila , Marika Vitikainen
IPC分类号： C12P21/02 , C12N9/48 , C07K14/37 , C12N15/80 , C12N9/00 , C12N9/16 , C12N9/24 , C12N9/42 , C12P21/00
CPC分类号： C12N9/48 , C07K14/37 , C12N9/00 , C12N9/16 , C12N9/2402 , C12N9/2437 , C12N9/244 , C12N9/2477 , C12N15/80 , C12P21/00 , C12Y302/01004 , C12Y302/01006 , C12Y302/01091 , Y02P20/52
摘要： The present invention relates to a method for genetically modifying a filamentous fungus host for improved protein production. The method comprises that a filamentous fungus host is genetically modified to overexpress or to be deficient of specific genes. The invention relates also to the modified hosts. Furthermore, the invention relates to a method for improved production or for producing an improved composition of proteins, such as cellulases, hemicellulases, other proteins involved in the degradation of lignocellulosic material, or other proteins, in a filamentous fungus host.
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公开(公告)号：US09487791B2
公开(公告)日：2016-11-08
申请号：US13701919
申请日：2011-05-30
申请人： Tiina Pakula , Markku Saloheimo , Mari Hakkinen , Ann Westerholm-Parvinen , Merja Penttila , Marika Vitikainen
发明人： Tiina Pakula , Markku Saloheimo , Mari Hakkinen , Ann Westerholm-Parvinen , Merja Penttila , Marika Vitikainen
CPC分类号： C12N15/80 , C12N9/2437 , C12N9/244 , C12N9/2445 , C12N9/2477 , C12N9/248 , C12N15/52 , C12Y302/01004 , C12Y302/01006 , C12Y302/01021 , C12Y302/01091
摘要： The present invention relates to a method for genetically modifying a filamentous fungus host for improved protein production. The method comprises that a filamentous fungus host is genetically modified to overexpress or to be deficient of specific genes. The invention relates also to the modified hosts. Furthermore, the invention relates to a method for improved production or for producing an improved composition of proteins, such as cellulases, hemicellulases, other proteins involved in the degradation of lignocellulosic material, or other proteins, in a filamentous fungus host.
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24.发明授权Yeast expressing cellulases for simultaneous saccharification and fermentation using cellulose 有权酵母表达纤维素酶用于同时糖化和发酵使用纤维素公开(公告)号：US09102955B2
公开(公告)日：2015-08-11
申请号：US13130549
申请日：2009-11-23
申请人： John McBride , Elena Brevnova , Mark Mellon , Allan Froehlich , Kristen Deleault , Vineet Rajgarhia , Riaan Den Haan , Merja Penttila , Marja Ilmen , Matti Siika-Aho , Jaana Uusitalo , Emily A. Stonehouse , Alan Gilbert , Haowen Xu , Deidre Willes , John Bardsley , Anu Koivula , Sanni Voutilainen
发明人： John McBride , Elena Brevnova , Mark Mellon , Allan Froehlich , Kristen Deleault , Vineet Rajgarhia , Riaan Den Haan , Merja Penttila , Marja Ilmen , Matti Siika-Aho , Jaana Uusitalo , Emily A. Stonehouse , Alan Gilbert , Haowen Xu , Deidre Willes , John Bardsley , Anu Koivula , Sanni Voutilainen
CPC分类号： C12P7/10 , C12N9/2437 , C12N9/2445 , C12N15/80 , C12P7/14 , C12P39/00 , C12Y302/01004 , C12Y302/01021 , C12Y302/01091 , Y02E50/16 , Y02E50/17
摘要： Host cells, comprising Kluveryomyces expressing heterologous cellulases produce ethanol from cellulose In addition, multiple host cells expressing different heterlogous cellulases can be co-cultured together and used to produce ethanol from cellulose The recombinant yeast strains and co-cultures of the yeast strains can be used to produce ethanol on their own, or can also be used in combination with externally added cellulases to increase the efficiency of sacchanfication and fermentation processes.
摘要翻译： 包含表达异源纤维素酶的克氏杆菌的宿主细胞从纤维素产生乙醇此外，可以将表达不同杂种纤维素酶的多个宿主细胞共同共培养并用于从纤维素产生乙醇可以使用酵母菌株的重组酵母菌株和共培养物 自己生产乙醇，也可以与外加纤维素酶组合使用，以提高发酵和发酵过程的效率。
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公开(公告)号：US08895273B2
公开(公告)日：2014-11-25
申请号：US13141736
申请日：2009-12-22
申请人： Harry Boer , Satu Hildich , Peter Richard , Merja Penttila
发明人： Harry Boer , Satu Hildich , Peter Richard , Merja Penttila
CPC分类号： C12P7/44 , C12N9/0006 , C12P7/58
摘要： The present invention relates to a method and microbial host strain for converting a hexuronic acid to a hexaric acid. In particular, the invention relates to the conversion of D-galacturonic acid to meso-galactaric acid (mucic acid). The invention also concerns an isolated nucleotide sequence. According to the present method a microbial host strain genetically modified to express uronate dehydrogenase enzyme (EC 1.1.1.203) is contacted with a biomaterial comprising hexuronic acid and the conversion products are recovered. By using the recombinant microorganisms of the present invention it is possible to treat biomaterials comprising hexuronic acids and thereby decrease the amount of hexuronic acids released to the environment.
摘要翻译： 本发明涉及一种将己糖醛酸转化为六酸的方法和微生物宿主菌株。 特别地，本发明涉及D-半乳糖醛酸转化为内消旋半乳糖酸（粘酸）。 本发明还涉及分离的核苷酸序列。 根据本方法，将遗传修饰以表达糖醛酸脱氢酶（EC 1.1.1.203）的微生物宿主菌与包含己糖醛酸的生物材料接触，并回收转化产物。 通过使用本发明的重组微生物，可以处理包含己糖醛酸的生物材料，从而减少释放到环境中的己糖醛酸的量。
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公开(公告)号：US20130084604A1
公开(公告)日：2013-04-04
申请号：US13701919
申请日：2011-05-30
申请人： Tiina Pakula , Markku Saloheimo , Mari Hakkinen , Ann Westerholm-Parvinen , Merja Penttila , Marika Vitikainen
发明人： Tiina Pakula , Markku Saloheimo , Mari Hakkinen , Ann Westerholm-Parvinen , Merja Penttila , Marika Vitikainen
IPC分类号： C12N15/52
CPC分类号： C12N15/80 , C12N9/2437 , C12N9/244 , C12N9/2445 , C12N9/2477 , C12N9/248 , C12N15/52 , C12Y302/01004 , C12Y302/01006 , C12Y302/01021 , C12Y302/01091
摘要： The present invention relates to a method for genetically modifying a filamentous fungus host for improved protein production. The method comprises that a filamentous fungus host is genetically modified to overexpress or to be deficient of specific genes. The invention relates also to the modified hosts. Furthermore, the invention relates to a method for improved production or for producing an improved composition of proteins, such as cellulases, hemicellulases, other proteins involved in the degradation of lignocellulosic material, or other proteins, in a filamentous fungus host.
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公开(公告)号：US20080176300A1
公开(公告)日：2008-07-24
申请号：US11947023
申请日：2007-11-29
申请人： Satu Hilditch , Merja Penttila , Peter Richard
发明人： Satu Hilditch , Merja Penttila , Peter Richard
摘要： A DNA molecule comprising a fungal gene encoding an enzyme protein capable of converting L-galactonic acid into L-threo-3-deoxy-hexulosonic acid has been cloned and heterologously expressed. The enzyme is involved in the metabolic conversion of sugar acids, which are present in biological waste material such as sugar beet pulp and other pectin comprising material. A microorganism genetically modified to effectively express said enzyme may be used in fermenting biomaterial to desired end products such as ethanol. Alternatively, microorganisms in which the gene has been inactivated may be used to produce L-galactonic acid, which accumulates when the expression of the gene is prevented.
摘要翻译： 包含编码能够将L-半乳糖转化为L-苏式-3-脱氧 - 六酮酸的酶蛋白的真菌基因的DNA分子已被克隆并异源表达。 该酶参与糖酸的代谢转化，其存在于生物废物如糖用甜菜浆和其它果胶包含材料中。 遗传修饰以有效表达所述酶的微生物可用于将生物材料发酵至期望的最终产物如乙醇。 或者，其中基因已经失活的微生物可以用于产生L-半乳糖酸，当预防基因的表达时它们积聚。
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公开(公告)号：US09499826B2
公开(公告)日：2016-11-22
申请号：US13701933
申请日：2011-05-30
申请人： Tiina Pakula , Markku Saloheimo , Mari Hakkinen , Ann Westerholm-Parvinen , Merja Penttila , Marika Vitikainen
发明人： Tiina Pakula , Markku Saloheimo , Mari Hakkinen , Ann Westerholm-Parvinen , Merja Penttila , Marika Vitikainen
CPC分类号： C12N15/80 , C07K14/37 , C12N9/16 , C12N9/2437 , C12N9/2477 , C12P1/02 , C12P21/00 , Y02P20/52
摘要： The present invention relates to a method for genetically modifying a filamentous fungus host for improved protein production. The method comprises that a filamentous fungus host is genetically modified to be deficient or to overexpress specific genes. The invention relates also to the modified hosts. Furthermore, the invention relates to a method for improved production or for producing an improved composition of proteins, such as cellulases, hemicellulases, other proteins involved in the degradation of lignocellulosic material, or other proteins, in a filamentous fungus host.
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29.发明申请Yeast Expressing Cellulases for Simultaneous Saccharification and Fermentation Using Cellulose 有权酵母表达纤维素酶用于同时糖化和发酵使用纤维素公开(公告)号：US20120129229A1
公开(公告)日：2012-05-24
申请号：US13130549
申请日：2009-11-23
申请人： John McBride , Elena Brevnova , Mark Mellon , Allan Froehlich , Kristen Deleault , Vineet Rajgarhia , Riaan Den Haan , Merja Penttila , Marja Ilmen , Matti Siika-Aho , Jaana Uusitalo , Emily A. Stonehouse , Alan Gilbert , Haowen Xu , Deidre Willes , John Bardsley , Anu Koivula , Sanni Voutilainen
发明人： John McBride , Elena Brevnova , Mark Mellon , Allan Froehlich , Kristen Deleault , Vineet Rajgarhia , Riaan Den Haan , Merja Penttila , Marja Ilmen , Matti Siika-Aho , Jaana Uusitalo , Emily A. Stonehouse , Alan Gilbert , Haowen Xu , Deidre Willes , John Bardsley , Anu Koivula , Sanni Voutilainen
CPC分类号： C12P7/10 , C12N9/2437 , C12N9/2445 , C12N15/80 , C12P7/14 , C12P39/00 , C12Y302/01004 , C12Y302/01021 , C12Y302/01091 , Y02E50/16 , Y02E50/17
摘要： Host cells, comprising Kluveryomyces expressing heterologous cellulases produce ethanol from cellulose In addition, multiple host cells expressing different heterlogous cellulases can be co-cultured together and used to produce ethanol from cellulose The recombinant yeast strains and co-cultures of the yeast strains can be used to produce ethanol on their own, or can also be used in combination with externally added cellulases to increase the efficiency of sacchanfication and fermentation processes
摘要翻译： 包含表达异源纤维素酶的克氏杆菌的宿主细胞从纤维素产生乙醇此外，可以将表达不同杂种纤维素酶的多个宿主细胞共同共培养并用于从纤维素产生乙醇可以使用酵母菌株的重组酵母菌株和共培养物 自己生产乙醇，也可以与外加的纤维素酶组合使用，以提高发酵和发酵过程的效率
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公开(公告)号：US20060084164A1
公开(公告)日：2006-04-20
申请号：US11252753
申请日：2005-10-19
申请人： Merja Penttila , Tiina Nakari-Setala , Richard Fagerstrom , Klaus Selber , Maria-Regina Kula , Markus Linder , Folke Tjerneld
发明人： Merja Penttila , Tiina Nakari-Setala , Richard Fagerstrom , Klaus Selber , Maria-Regina Kula , Markus Linder , Folke Tjerneld
CPC分类号： C07K7/06 , C07K1/14 , C07K7/08 , C07K14/37 , C07K2319/00 , C07K2319/02
摘要： The present invention relates to isolation and purification of protein in aqueous two-phase systems (ATPS). Specifically, the invention provides processes for partitioning of proteins of interest in ATPS by fusing said proteins to targeting proteins which have the ability of carrying said protein into one of the phases.
摘要翻译： 本发明涉及水相两相体系（ATPS）中蛋白质的分离和纯化。 具体地，本发明提供了通过将所述蛋白质融合到具有携带所述蛋白质到其中一个相的能力的靶向蛋白质来分配ATPS中感兴趣的蛋白质的方法。
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